• Title/Summary/Keyword: Biology and Life Science

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Analysis of Intragenic Ds Transpositions and Excision Events Generating Novel Allelic Variation in Rice

  • Park, Soon Ju;Piao, Hai Long;Xuan, Yuan-hu;Park, Sung Han;Je, Byoung Il;Kim, Chul Min;Lee, Eun Jin;Park, Soo Hyun;Ryu, ByeongChan;Lee, Kon Ho;Lee, Gi Hwan;Nam, Min Hee;Yeo, Un Sang;Lee, Myung Chul;Yun, Doh Won;Eun, Moo Young;Han, Chang-deok
    • Molecules and Cells
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    • v.21 no.2
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    • pp.284-293
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    • 2006

  • Even though Ac/Ds gene-tagging systems have been established in many higher plants, maize is the only major plant in which short-distance transposition of Ac/Ds has been utilized to probe gene function. This study was performed to evaluate the efficiency of obtaining new alleles and functional revertants from Ds insertion loci in rice. By analyzing 1,580 plants and the progeny of selected lines, the insertion sites and orientations of Ds elements within 16 new heritable alleles of three rice loci were identified and characterized. Intragenic transposition was detected in both directions from the original insertion sites. The closest interval was 35 bp. Three of the alleles had two Ds elements in cis configuration in the same transcription units. We also analyzed the excision footprints of intragenic and extragenic transpositions in Ds-inserted alleles at 5 loci. The 134 footprints obtained from different plants revealed predominant patterns. Ds excision at each locus left a predominant footprint at frequencies of 30-75%. Overall, 66% of the footprints were 7-bp additions. In addition, 16% of the excisions left 0-, 3-, 6-, and 9-bp additions with the potential of conserving reading frame.

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Review of Biology Articles Published In "Vacation Life" Distributed in Middle School In Korea (우리나라 중학교 "방학생활"에서 생물분야에 대한 해설(제2보(第二報)) (1980년 겨울-1981년 여름))

  • Lee, Woong-Jik
    • Journal of The Korean Association For Science Education
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    • v.8 no.2
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    • pp.53-56
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    • 1988

  • The Korean Federation of Education Association has published "Vacation Life" to promote self-teaching of middle school students during the summer and winter vacation of middle school students every year. The book include whole subjecy offered in the middle school. However subject of science(Physics, chemistry, biology and earth science) is more emphasized. This article described brief summary of the contents of biology branch published in winter "Vacation Life", 1980 and summer "Vacation Life" 1981.

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Interaction of Microtubule-associated Protein 1B Light Chain(MAP1B-LC1) and p53 Represses Transcriptional Activity of p53

  • Kim, Jung-Woong;Lee, So-Youn;Jeong, Mi-Hee;Jang, Sang-Min;Song, Ki-Hyun;Kim, Chul-Hong;Kim, You-Jin;Choi, Kyung-Hee
    • Animal cells and systems
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    • v.12 no.2
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    • pp.69-75
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    • 2008

  • The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses, and can trigger apoptosis in many cell types including neurons. In this study, we have shown that Microtubule-associated protein 1B(MAP1B) light chain interacts with tumor suppressor p53. MAP1B is one of the major cytoskeletal proteins in the developing nervous system and essential in forming axons during elongation. We also demonstrate that both p53 and MAP1B-LC1 interact in the nucleus in HEK 293 cells. Indeed, we show that the MAP1B-LC1 negatively regulates p53-dependent transcriptional activity of a reporter containing the p21 promoter. Consequently, MAP1B light chain binds with p53 and their interaction leads to the inhibition of doxorubicin-induced apoptosis in HEK 293 cells. Furthermore, these examinations might be taken into consideration when knock-down of MAP1B-LC1 is used as a cancer therapeutic strategy to enhance p53's apoptotic activity in chemotherapy.

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