• 한국해양바이오학회지
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• 제1권2호
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• pp.59-62
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• 2006

Seaweed biotechnology is a multidisciplinary subject to produce food, pharmaceuticals, chemicals, and environmental remediation materials from seaweed resources. It uses various techniques of cell culture, enzyme reaction and genetic manipulation to increase the production efficiency of useful seaweeds or their products. Firstly, an overview of key topics will be introduced in the fields of seaweed tissue culture, strain improvement, genetic analysis briefly as basic techniques. Secondly, some biologically active substances such as anti-inflammatory and antifouling substances that have been screened in my laboratory will be focused.

• Journal of Microbiology and Biotechnology
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• 제16권5호
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• pp.651-660
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• 2006

Natural products are a rich source of biologically active small molecules and a fertile area for lead discovery of new drugs [10, 52]. For instance, 5% of the 1,031 new chemical entities approved as drugs by the US Food and Drug Administration (FDA) were natural products between 1981 and 2002, and another 23% were natural product-derived molecules [53]. These molecules have evolved through millions of years of natural selection to interact with biomolecules in the cells or organisms and offer unrivaled chemical and structural diversity [14, 37]. Nonetheless, a large percentage of nature remains unexplored, in particular, in the marine and microbial environments. Therefore, natural products are still major valuable sources of innovative therapeutic agents for human diseases. However, even when a natural product is found to exhibit biological activity, the cellular target and mode of action of the compound are mostly mysterious. This is also true of many natural products that are currently under clinical trials or have already been approved as clinical drugs [11]. The lack of information on a definitive cellular target for a biologically active natural product prevents the rational design and development of more potent therapeutics. Therefore, there is a great need for new techniques to expedite the rapid identification and validation of cellular targets for biologically active natural products. Chemical genomics is a new integrated research engine toward functional studies of genome and drug discovery [40, 69]. The identification and validation of cellular receptors of biologically active small molecules is one of the key goals of the discipline. This eventually facilitates subsequent rational drug design, and provides valuable information on the receptors in cellular processes. Indeed, several biologically crucial proteins have already been identified as targets for natural products using chemical genomics approach (Table 1). Herein, the representative case studies of chemical genomics using natural products derived from microbes, marine sources, and plants will be introduced.

• 한국식품영양과학회지
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• 제27권1호
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• pp.121-125
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• 1998

The effects of various cooking methods (blanching, microwave heating, and deep-fat frying) on biologically active components of Angelica keiskei were determined by HPLC. Cynharoside, the biologically active component of Angelica keiskei leaves was 4.82%, which was rapidly decreased by blanching, showing 3.79%, 2.59% and 1.74% at 1 min, 2min and 3min, respectively. Microwave heating also decreased the cynaroside contents slowly by 2 min and rapidly by 3min, respectively. Microwave heating also decreased the cynaroside contents slowly by 2min and rapidly by 3 min, showing 4.25% at 1 min, 3.38% at 2 min, and 1.49% at 3 min. Among the cooking methods tested, deep-fat frying was shown to preserve the cynaroside most. Only 3.90% of cynaroside was lost by 5 min frying. The decrease in cynaroside in each cooking method was supposed to be due to the conversion of cynarside, a glycoside of flavonoid, into luteolin through lysis of glucose at C-7 position on cynaroside.

• 셀메드
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• 제6권4호
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• pp.22.1-22.19
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• 2016

The biologically active compounds derived from different natural organisms such as animals, plants, and microorganisms like algae, fungi, bacteria and merine organisms. These natural compounds possess diverse biological activities like anthelmintic, antibacterial, antifungal, antimalarial, antiprotozoal, antituberculosis, and antiviral activities. These biological active compounds were acted by variety of molecular targets and thus may potentially contribute to several pharmacological classes. The synthesis of natural products and their analogues provides effect of structural modifications on the parent compounds which may be useful in the discovery of potential new drug molecules with different biological activities. Natural organisms have developed complex chemical defense systems by repelling or killing predators, such as insects, microorganisms, animals etc. These defense systems have the ability to produce large numbers of diverse compounds which can be used as new drugs. Thus, research on natural products for novel therapeutic agents with broad spectrum activities and will continue to provide important new drug molecules.

• KSBB Journal
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• 제8권3호
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• pp.287-294
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• 1993

사람의 뇨로부터 유용한 단백질을 동시에 분리하 기 위한 목적으로 효과적인 통합 분리정제 공정이 고안되었다. Ultrafiltration 방법을 이용한 농축과정 과 pH침전법을 전처리 단계로 사용하였고, 이어서 g gel filtration과 흡착, 이온교환, 친화, 그리고 역상 C column을 선택적으로 혼합한 chromatography를 수행하였다. 이 공정으로 정제한 urokinase, epider­m mal growth factor, albumin은 각각 SDS-poly­a acrylamide gel 전기이동상에서 단일의 단백질 띠로 이동하였고, 자신의 단백질 활성을 유지하고 있었다. 이들 세 가지 목적 단백질의 전체 수율은 각각 48 % %, 17%, 46%로 나타났다.

• 기술사
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• 제26권4호
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• pp.40-45
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• 1993

• 기술사
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• 제27권4호
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• pp.95-97
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• 1994

• 대한약학회:학술대회논문집
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• 대한약학회 2001년도 Proceedings of the Pharmaceutical Society of Korea
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• pp.84-85
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• 2001

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1995년도 The 5th International Symposium on the Development of Anti Cancer Resources From Plants 및 1995년도 동양자원식물학회 정기학술 발표회
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• pp.5-5
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• 1995

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1996년도 International Symposium on the Development of Anti Cancer Resources From Plants및 1996년도 한국자원식물학회 정기학술 발표회
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• pp.3-7
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• 1996