• Applied Biological Chemistry
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• v.16 no.1
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• pp.12-17
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• 1973

Alliin was isolated from deep-frozen garlic bulbs and purified into crystalline form. Purity of isolated alliin was assured by melting point determination and thin-layer chromatography. Sulfoxide bond and functional groups of amino acid were confirmed with IR spectrum, and vinyl bond with NMR spectrum. Molecular weight, allyl and other bonds were confirmed with MS spectrum.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.35 no.1
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• pp.41-49
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• 1999

This paper describes to analyze the underwater ambient noise and biological noise of cultivating fishes in the fish farm cages at the seawater Tongyong-kun, KyongNam and lake of Chungju, Chech'on, ChungBuk from 10 to 19 Oct. 1997, in order to find out the characteristics of these noises. The results obtained were as follows; (1) The ambient noise around the fish farm cages at lake of Chungju was 10~200Hz frequency range, 70~105dB spectrum level. The central frequency was 50~70Hz, changing of ambient noise was getting bigger than 10~200Hz in 200Hz~2kKz frequency by wind, water current. (2) The frequency of noise source around the fish farm cage at the seawater of Tongyong-kun was 20~200Hz, spectrum level was 80~100dB while feed factory was working around the fish farm cage. When feed factory did not work, noise source was 10~600Hz frequency range, 70~90dB spectrum level. It was 10dB less than that of while feed factory was working, and then the central frequency was 70Hz. (3) The vessel noise of excursion ship had changed largely at 100dB spectrum level in 10~500Hz frequency band, and the fishing boat had 20Hz~2kHz frequency range. (4) The biological noise in the fish farm cage at lake of Chungju, which was feeding of Cyprinus carpio, 2was 10~30Hz frequency, 70~104dB spectrum level. The central frequency was 75Hz. The biological noises in the fish farm cage at the seawater of Tongyong-kun, which were feeding and swimming noise, had very different spectrum pattern by species, and the frequency band was 10~800Hz.

• Journal of Biomedical Engineering Research
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• v.4 no.1
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• pp.29-36
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• 1983

In this paper, center frequency down slift of ultrasonic echo signals which for the measurements of frequency dependent attenuation in the biological tissue are estimated. Center frequency down shift of echo-signals are estimated after signal spectrum analysis of whole echo-signals. In case of signal spectrums are simple, estimation of down shift frequency is very simple and in case of complicate spectrum, estimation of down shift frequency is depend on spectral shape. In case of unable to estimate, frequency dependence of medium is nonlinear(n) 1), in which upper shift of spectrums are presented. In case of unable to estimate, spectrum analysis are performed at local position. At consquence, we know that spectral dispersions are caused complicately by biological tissue layer.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.521-524
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• 2000

Nearinfra-red(NIR) reflectance NIR a spectra (400 to 2,100 nm) were collected on 32 beef samples to find feasibility of predicting beef tenderness. The study to predict beef tenderness was accomplished with the stepwise second differential data of the collected NIR spectra. Beef tenderness was measured by Warner-Bratzler(WB) shear force using a Universal Testing Machine(UTM). After modeling the relation between Warner-Bratzler shear force and NIR spectrum of 19 samples among the 32 beef samples, the verification was carried out through predicting the other 13 samples. The SEC and R$^2$ values in the prediction equation were 9.07(N) and 0.6463, respectively. The SEP and R$^2$ were 14.8(N) and 0.7082 (wave length 552 nm, 1988 nm) respectively. The result implied that it was possible to predict the beef tenderness using NIR spectrum and that the tenderness could be predicted non-destructively in real time.

• Applied Biological Chemistry
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• v.37 no.5
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• pp.392-396
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• 1994

The toxic substance causing blown blotching on the mushroom cap was extracted and purified from the culture broth of P.tolaasii. Purification and identification of the toxic compound was carried out with the silica gel chromatography, mass spectrum and NMR and appeared to be an aminobenzene in amylamine group. The purified toxic substance showed UV spectrum at 234nm and melting point at $76^{\circ}C$, respectively.

• Journal of Photoscience
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• v.9 no.2
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• pp.460-462
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• 2002

Absorption spectra of biological specimens in the soft X-ray region have been presented with special reference to the XANES (X-ray absorption Near Edge Structure) of constituent elements. Absorption spectrum in this wavelength region is characterized by the absorption edges from which elemental content could be derived. In addition, XANES has a characteristic profile for chemical environment around the element such as chemical bond. Using the specific absorption peak we can assign not only the chemical bond but also molecules having such a chemical bond. In the present paper, absorption spectrum of DNA was measured in the wavelength range from 1.5nm to 5nm. Spectrum of Chinese Hamster Ovary (CHO) cells was compared with the DNA spectrum. XANES were distinct at the K absorption edges of major elements, C, N and O. In the spectrum of the cells prominent peaks at the L absorption edge of minor element Ca were also detectable. XANES profiles in small local areas in a cell could also be measured in combination with X-ray microscopy. These give information about local chemical environment in a cell. XANES at the phosphorus K absorption edge in a human HeLa cell was successfully obtained corresponding to a sharp and intensive XANES peak of DNA.

• Journal of the Korea Institute of Military Science and Technology
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• v.14 no.2
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• pp.289-298
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• 2011

TMAH-based Py-MS has been investigated to apply for a real-time classification of biological agents in the field. Acquiring reproducible data from mass spectrometry is a key to biological detection in the field. Nevertheless, it has been little studied on what factors could affect to the reproducibility of the TMAH-based Py-MS spectrum patterns. Given the TMAH-based Py-MS applied to the field system, several factors which could affect to the reproducible pattern of TMAH-based Py-MS spectra are needed to be examined, including changes in TMAH injection volume, growth temperature for microorganism, and number of cells collected in pyrolyzer, and implication of stabilizer used for lyophilization. This study showed that the reproducibility of the spectrum patterns was significantly hindered by changes in TMAH concentration and cell number, and stabilizer implication but not by growth temperature. Among those at low TMAH concentration(0.015m) was not observed the significant alterations of the spectrum pattern even when its injection volume was changed, yet was in different cell numbers and stabilizer implication.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.155.1-155.1
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• 2015

Nowadays, Plasma has been used in biological, medical such as wound healing, plant grow, killing cancer. When plasma generated, UV light and ROS(Reactive oxygen species), RNS(Reactive nitrogen species) can generated and those things effect to biological material. So we made simple plasma device using needle type of electrode and generated plasma. We used three kinds of gas and measured applied voltage and current. Also we observed optical emission spectrum. Using deuterium ramp, we can observed absorption spectrum and calculated radical density by lambert-beer's law. It is around ~1016cm3. And we can see the time-resolved absorption spectrum from monochromator, PMT(photo multiply tube), IV-converter, oscilloscope.

• Journal of Microbiology and Biotechnology
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• v.6 no.4
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• pp.265-269
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• 1996

An isolate of Streptomyces rochei synonym was found to produce antibiotics with narrow anti-microbial spectrum against Streptococcus and Xanthomonas sp. Among the antibiotic complex produced by the strain, the main active compound was isolated, and its physico-chemical properties and biological activities were investigated. Molecular weight of the compound was determined to be ${[M+H]}^+$ 797 (FAB-MS). UV, $^1H \;and\;^{13}C$ NMR, and IR spectra suggested that the compound is a kirromycin-like aurodox group antibiotic. However, the anti-microbial spectrum of the main compound was slightly different from that of kirromycin. In addition, it was newly found that kirromycin showed a selective anti-microbial activity against Streptococcus pyogenes and phytopathogenic Xanthomonas sp.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.560-568
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• 2000

The aims of this research were to investigate the preprocessing effect of spectrum data on prediction performance and to develop a robust model to predict SSC in intact apple. Spectrum data of 320 Fuji apples were measured with the on-line transmittance measurement system at the wavelength range of 550∼1100nm. Preprocess methods adopted for the tests were Savitzky Golay, MSC, SNV, first derivative and OSC. Several combinations of those methods were applied to the raw spectrum data set to investigate the relative effect of each method on the performance of the calibration model. PLS method was used to regress the preprocessed data set and the SSCs of samples, and the cross-validation was to select the optimal number of PLS factors. Smoothing and scattering corection were essential in increasing the prediction performance of PLS regression model and the OSC contributed to reduction of the number of PLS factors. The first derivative resulted in unfavorable effect on the prediction performance. MSC and SNV showed similar effect. A robust calibration model could be developed by the preprocessing combination of Savitzky Golay smoothing, MSC and OSC, which resulted in SEP= 0.507, bias=0.032 and R$^2$=0.8823.