• The Korean Journal of Mycology
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• 제48권4호
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• pp.407-414
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• 2020

To replace the sawdust in the growth medium of oyster mushroom to utilize its by-product spent mushroom substrates (SMS) as feed for ruminant, we performed cultivation test using cotton seed hull pellet (CSHP), corn stalk pellet (CSP), corncob (CC), and analyzed the feed chemical properties of those SMS. As a result of cultivation test, CC and CSHP treatment took 27 days for spawn run, 4 days for primordium formation, and 3 days for development fruiting bodies, resulting in a total cultivation period of 34 days. The yield per bottle was 134 g for CC treatment, similar to 130 g for control, while CSHP treatment (112 g) and CSP treatment (68 g) were lower than that of control. The highest biological efficiency (BE) was shown in CC treatment as 80.1%, which was 11.4% higher than 68.7% of control. The SMS of CC treatment had a relatively low content of neutral detergent fiber and acid detergent fiber, and in particular, lignin content was the lowest and crude protein content was the highest among other treatments. Therefore, CC as a substitute material for sawdust was capable of stable mushroom production and excellent nutritional value as a feed for its by-products.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제22권3호
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• pp.51-57
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• 2021

The seawater cooling system of naval ships is installed to remove the toxic substances generated by CBR (Chemical, Biological, and Radiological) warfare and reduce the infrared signature of naval ships from outside the hull. The dispersion range of the nozzle is determined according to the injection pressure of seawater and the nozzle type. Therefore, it is necessary to select the appropriate injection pressure and design the optimal nozzles to increase the seawater dispersion area and maximize the efficiency of the cooling system. In this study, the applying feasibility of 3D printing technology to produce an injection nozzle for the seawater cooling system was examined. To this end, the extruded plastic specimens were fabricated by 3D printing, and the physical properties of the specimens were estimated through tensile testing. After this, the strain and stress of the nozzle as a function of the pressure were simulated by applying the estimated results to the finite element analysis. The finite element analysis results showed that the nozzle remained within the elastic range at the optimal pressure. The nozzle was estimated to be structurally stable, and the possibility of this study was confirmed.

• Korean Journal of Optics and Photonics
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• 제33권4호
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• pp.177-186
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• 2022

The performance of a surface plasmon resonance sensor is evaluated based on the sensitivity (nm/RIU) and sharpness from the full width at half maximum (FWHM) and the peak depth of a resonance peak. These factors are determined by the materials and conformational properties of the sensing structure. In this paper, we investigated an optimized insulator-metal-insulator (IMI) multilayer-based surface plasmon resonance sensor structure to simultaneously achieve high sensitivity, narrow FWHM, and deep peak depth while using gold for the metallic film layer which occurs peak broadening. By adopting the optimized structure, sensitivity of 8,390 nm/RIU, FWHM of 11.92 nm, and a resonance peak depth of 93.1% were achieved for 1.45-1.46 refractive index variation of the sensing layer. With the suggested structure conformation, high sensitivity and resolution of sensing performance can be achieved.

• Journal of Biomedical Engineering Research
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• 제43권3호
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• pp.136-146
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• 2022

In this study, it is intended to provide basic data that can help develop a cardiovascular simulator for performance evaluation of pulse wave detectors by identifying the development status of domestic and overseas cardiovascular simulators. A total of 119 papers were selected by excluding duplicate literature, gray literature, and literature not related to a cardiovascular simulator. Based on the selected literature, the research trend of cardiovascular simulators was analyzed. As a result of analyzing the purpose of the study, most of the simulators were developed to evaluate the hemodynamic properties of artificial hearts and valves. In addition, it was used for simulation evaluation or hemodynamic studies such as pulse wave studies. As a result of analyzing configurations of the simulators, a heart most often consisted of only one left ventricle. For blood vessels, the Windkessel model was most often constructed using chambers and valves. In most studies, blood was reproduced by mixing glycerin and water to reproduce both density and viscosity. In addition, as a result of analysis from the perspective of medical device performance evaluation, simulators for evaluating artificial heart and artificial valves have been studied a lot, whereas simulators for blood pressure, pulse wave, and blood flow devices have been relatively insignificant. Based on the review results, we suggested considerations when developing a simulator for performance evaluations of a pulse wave detector.

• Journal of Life Science
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• 제32권1호
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• pp.44-50
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• 2022

The migration and proliferation of keratinocytes are key events in re-epithelization, itself a major phase in the wound healing process. Cuscuta japonica Choisy (CJC) is used as a traditional medicine to improve liver, heart, and intestinal function, and its extracts are reported to have various biological properties such as whitening, anti-oxidancy, and an anti-acne effect. However, it is not yet known in particular whether or not CJC essential oil (CJCEO) affects skin regeneration. In the present study, we isolated CJCEO by solvent extraction and tested its effect on wound healing responses using normal human keratinocytes, namely HaCaT cells. We found that CJCEO induced proliferation as well as migration in HaCaT cells in a dose-dependent manner. Compared with a control group, CJCEO treatment at 250 ㎍/ml increased proliferation by 239.98±5.51% in HaCaT cells in a dose and migration by 124.86±6.06%. Moreover, the oil induced sprout outgrowth and, at 250 ㎍/ml, increased collagen synthesis by 148.56±15.47% in HaCaT cells. These results demonstrate that CJCEO may promote skin regeneration and wound healing by increasing the migration, proliferation, and collagen synthesis of HaCaT cells. We therefore suggest that CJCEO could be used as a cosmetic material.

• Journal of Conservation Science
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• 제37권6호
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• pp.778-790
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• 2021

The Stone Seated Buddha Triad and Stone Standing Buddha in Bijung-ri are state-designated heritage (treasure) statues having the Buddha style of the Goryeo dynasty from the 6th century. Conservation scientific investigations were conducted to understand the preservation status of these stone Buddha statues and to establish a conservation plan. The Stone Seated Buddha Triad and Stone Standing Buddha are composed of fine-medium grained biotite granite, which is considered to be of the same origin owing to their low magnetic susceptibility distribution of less than 0.2 (×10^-3 SI unit) and similar mineral characteristics. The Stone Seated Buddha Triad has highly homogenous mineral composition and particle size, whole-rock magnetic susceptibility, and geochemical characteristics very similar to those of the nearby outcrop. It was confirmed that a combination of physical, chemical, and biological factors affects the Stone Buddha statues. In particular, both the Stone Seated Buddha Triad and Stone Standing Buddha tend to be chipped off from the front and cracked and scaled from the back. The Stone Standing Buddha located outdoors experiences granularity decomposition and black algae formation, which accelerate the weathering under unfavorable conservation environments. The result of non-destructive physical property diagnosis using ultrasonic velocity showed that both the Stone Seated Buddha Triad and Stone Standing Buddha have been completely weathered (CW), indicating very poor physical properties.

• Journal of the Korean Wood Science and Technology
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• 제30권1호
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• pp.25-33
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• 2002

Improvement of density profile and sound absorption capability of particleboard was attempted. Three types of hot pressing methods examined ; flat-platen pressing method (A-type pressing), hot pressing in forming box (B-type pressing), and hot pressing set up jagged caul in forming box (C-type pressing). The raw materials were larch(Larix leptolepis (S, et. Z.) Gorden) shavings, phenol formaldehyde resin, and the particleboard perforated with stair type. The physical and mechanical properties such as specific gravity, bending strength (MOR), internal bonding strength (IB) and sound absorption coefficients were examined. The results are summarized as follows : 1) The MOR and internal bonding strength of the board pressed in forming box were higher than those of flat-platen pressed board. 2) The minimum density to average density ratio in thickness direction which pressed in forming box showed about 923%, in the case of 35 mm commercial particleboard and 50 mm flat-platen pressed board, its values showed about 66.4% and 865% respectively. 3) Sound absorption coefficients of the particleboard perforated with stair type were higher than those of flat-plated pressed board and commercial particleboard.

• Journal of Veterinary Science
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• 제21권5호
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• pp.64.1-64.14
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• 2020

Background: Canine distemper virus (CDV) infection results in high morbidity and mortality in dogs. There has been no report about Isolation of Korean CDV since 1980 in Korea. Objectives: To investigate the biological properties and the genetic characterization of Korean CDV. Methods: Vero cells expressing dog signaling lymphocyte activation molecule (dSLAM) gene named as Vero/dSLAM were used to isolate CDV using 17 samples. Diagnostic methods such as cytopathic effects, immunofluorescence assay, peroxidase linked assay, electron microscopy, rapid immunodiagnostic assay, and reverse transcription polymerase chain reaction were used to confirm the Korean CDV isolate as a CDV. The genetic analysis was performed through cloning and sequencing of hemagglutinin gene of CDV isolate. Results: A virus propagated in Vero/dSLAM cell was confirmed as CDV (CD1901 strain) based on the above methods. The CD1901 strain showed the highest viral titer (10^5.5 50% tissue culture infectious dose [TCID₅₀]/mL) in the Vero/dSLAM cells at 4 days post inoculation, but did not form a fork on chorioallantoic membrane of 7-day-old egg. Ribavirin, a nucleotide analogue anti-viral agent, inhibits moderately the Korean CDV propagation in the Vero/dSLAM cells. The nucleotide and amino acid sequences of the H gene of CD1901 strain were compared with those of other CDV strains. The CD1901 strain belonged to Asia 1 group and had the highest similarity (99.9%) with the BA134 strain, which was isolated in China in 2008. Conclusions: We constructed successfully Vero/dSLAM and isolated one Korean CDV isolate (CD1901 strain) from a naturally infected dog. The CD1901 strain belonged to Asia 1 genotype.

• Journal of Life Science
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• 제32권2호
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• pp.94-100
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• 2022

Chronic infection by hepatitis B virus (HBV) greatly increases the risk for liver cirrhosis and hepatocellular carcinoma (HCC). The outcome of HBV infection is shaped by the complex interplay of the mode of transmission, host genetic factors, viral genotype, adaptive mutations, and environmental factors. The pregenomic RNA transcription of HBV for their replication is regulated by the core promoter activation. Core promoter mutations have been the reason for acute liver failure and are associated with HCC development. We obtained HBV genes from a patient in Myanmar who was infected with HBV and identified gene variations in the core promoter region. For measuring the relative transactivation activity of the core promoter, we prepared the core-promoter reporter construct. Among the gene variations of the core promoter, the mutations of C1731T and G1806A were associated with increase in the transactivation of the HBV core promoter. Through computer analysis for searching for a tentative transcription factor binding site, we showed that the mutations of C1713T and G1806A newly created C/EBPβ and XBP1-responsive elements of the core promoter, respectively. The ectopic expression of C/EBPβ largely increased the HBV core promoter containing the C1713T mutation and that of XBP1 activated the M95 promoter containing the G1806A mutation. Our efforts to treat and prevent HBV infections are hampered by the emergence of drug-resistant mutations and vaccine-escape mutations. Our results provide the biological properties and clinical significance of specific HBV core promoter mutations.

• Korean Journal of Heritage: History & Science
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• 제55권3호
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• pp.60-72
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• 2022

A number of wooden built heritage remain in Korea, and most have been damaged by various biological factors including termite. Owing to the irreversible damage caused by termites, wooden built heritage are losing their authenticity and structural stability. In this study, Area-Wide Integrated Termite Management(AW-ITM) was proposed to prevent termite damage. First, to understand the locational characteristics of these sites, the distance from adjacent forests and surrounding forest areas was analyzed for 182 national designated wooden built heritage(national treasures, treasures) using the Geographic Information System(GIS). By analyzing existing pest control projects(2003-2020) and the components of the ITM, the characteristics of termite control for cultural heritages were determined. Based on these results, the cultural heritage sites and their surrounding spaces were divided into three areas, and the types of cultural properties were divided into six types according to the location and number of buildings. Along with this, termite control measures were proposed for each area and type. The concept of AW-ITM has been partially applied to the "Comprehensive Control of Termites in wooden built heritages Sites" by the Cultural Heritage Administration. Caution must be taken with regard to the establishment of a cultural heritage management policy; AW-ITM should be applied on a trial basis with the results then being carefully analyzed and reflected in the establishment of policies pertaining to the conservation management of cultural heritage.