• 제목/요약/키워드: Biological evolution

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A Review of Chlorine Evolution Mechanism on Dimensionally Stable Anode (DSA®) (DSA 전극에서 염소 발생 메커니즘)

  • Kim, Jiye;Kim, Choonsoo;Kim, Seonghwan;Yoon, Jeyong
    • Korean Chemical Engineering Research
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    • v.53 no.5
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    • pp.531-539
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    • 2015
  • Chlor-alkali industry is one of the largest electrochemical processes which annually producing 70 million tons of sodium hydroxide and chlorine from sodium chloride solution. $DSA^{(R)}$ (Dimensionally Stable Anodes) electrodes such as $RuO_2$ and $IrO_2$, which is popular in chlor-alkali process, have been investigated to improve the chlorine generation efficiency. Although DSA electrode has been developed with various researches, understanding of the chlorine evolution mechanism is essential to the development of highly efficient DSA electrode. In this review paper, chlorine generation mechanisms are summarized and that of key factors are identified to systematically understand the chlorine generation mechanism. Rate determining step, effect of pH, reaction intermediate, and electrode crystal structure were intensively overviewed as key factors of the chlorine mechanism.

Evaluation of Luminescent P450 Analysis for Directed Evolution of Human CYP4A11

  • Choi, Seunghye;Han, Songhee;Lee, Hwayoun;Chun, Young-Jin;Kim, Donghak
    • Biomolecules & Therapeutics
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    • v.21 no.6
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    • pp.487-492
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    • 2013
  • Cytochrome P450 4A11 (CYP4A11) is a fatty acid hydroxylase enzyme expressed in human liver. It catalyzes not only the hydroxylation of saturated and unsaturated fatty acids, but the conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), a regulator of blood pressure. In this study, we performed a directed evolution analysis of CYP4A11 using the luminogenic assay system. A random mutant library of CYP4A11, in which mutations were made throughout the entire coding region, was screened with luciferase activity to detect the demethylation of luciferin-4A (2-[6-methoxyquinolin-2-yl]-4,5-dihydrothiazole-4-carboxylic acid) of CYP4A11 mutants in Escherichia coli. Consecutive rounds of random mutagenesis and screening yielded three improved CYP4A11 mutants, CP2600 (A24T/T263A), CP2601 (T263A), and CP2616 (A24T/T263A/V430E) with ~3-fold increase in whole cells and >10-fold increase in purified proteins on the luminescence assay. However, the steady state kinetic analysis for lauric acid hydroxylation showed the significant reductions in enzymatic activities in all three mutants. A mutant, CP2600, showed a 51% decrease in catalytic efficiency ($k_{cat}/K_m$) for lauric acid hydroxylation mainly due to an increase in $K_m$. CP2601 and CP2616 showed much greater reductions (>75%) in the catalytic efficiency due to both a decrease in $k_{cat}$ and an increase in Km. These decreased catalytic activities of CP2601 and CP2616 can be partially attributed to the changes in substrate affinities. These results suggest that the enzymatic activities of CYP4A11 mutants selected from directed evolution using a luminogenic P450 substrate may not demonstrate a direct correlation with the hydroxylation activities of lauric acid.

One Chrysidid Wasp, Holopyga generosa, New to Korea

  • Bang, Seong-Ho;Ha, Hyeong-Hwa;Kim, Jeong-Kyu
    • Animal Systematics, Evolution and Diversity
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    • v.27 no.3
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    • pp.271-273
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    • 2011
  • The Korean occurrence of Holopyga generosa (F$\ddot{o}$rster, 1853) is reported here for the first time. The genus is also new to Korea. This species might be easily distinguished from other Holopyga species by its relatively long hairs on the head and mesosoma. Diagnosis and digital images are provided.

A Taxonomic Review of the Genus Lycocerus (Cantharidae: Coleoptera) in Korea, with a Newly Recorded Species, Lycocerus striatus

  • Kang, Tae Hwa;Kim, Tae Woo;Seo, Hong Yul
    • Animal Systematics, Evolution and Diversity
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    • v.33 no.3
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    • pp.203-210
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    • 2017
  • A taxonomic review of the Korean Lycocerus (Cantharidae, Coleoptera) was presented. Through this study, total of four species were found from Korea including a species new to Korea, Lycocerus striatus. We newly provided a key to the Korean Lycocerus species and the distributional map on each species in this study.