• Title/Summary/Keyword: Biological Science

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A report on 20 unrecorded bacterial species of Korea isolated from soil in 2021

  • Ji Yeon, Han;Oung Bin, Lim;So-Yi, Chea;Hyosun, Lee;Ki-Eun, Lee;In-Tae, Cha;Won-Jae, Chi;Dong-Uk, Kim
    • Journal of Species Research
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    • v.11 no.4
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    • pp.310-320
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    • 2022
  • As a subset study to discover indigenous prokaryotic species in Korea, we isolated 20 bacterial strains and assigned them to the phyla Actinobacteria, Bacteroidota, Firmicutes, and Proteobacteria. From the high 16S rRNA gene sequence similarity (≥98.7%) and formation of a robust phylogenetic clades, we determined that each strain belonged to independent, predefined bacterial species. There are no official reports of these 20 species in Korea; therefore, 7 strains of the Actinobacteria, 2 strain of the Bacteroidota, 3 strains of the Firmicutes, and 8 strains of the Firmicutes are described in Korea for the first time. Gram reaction, colony and cell morphology, basic biochemical characteristics, and isolation sources are also described in the species description section.

Quantification of the galactose-operon mRNAs 5 bases different in their 5'-ends

  • Ji, Sang-Chun;Wang, Xun;Jeon, Heung-Jin;Yun, Sang-Hoon;Lee, Hee-Jung;Lim, Heon-M.
    • BMB Reports
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    • v.43 no.7
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    • pp.474-479
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    • 2010
  • Three assay methods for quantification of the two galactose-operon mRNAs that only differ by 5 bases in their 5'-end are presented. The 5' ends of each mRNA were extended by ligating the 3'-end of the abundant 5S rRNA. This ligation extends the 5' ends of the two gal mRNAs long enough to be distinguished by the specific PCR primers in the following quantification reactions. Quantification of the corresponding cDNAs was performed either by primer extension assay or real-time qPCR. To circumvent the problem of the RNA ligation reaction (i.e. very low ligation efficiency), we devised a new method that employs real-time qPCR directly for the quantification of the gal transcripts which differ by 5 bases in their 5'-ends.

A report of 10 unrecorded bacterial species of Korea, isolated from agricultural soil in 2022

  • Oung Bin Lim;Ji Soo Lee;Hyosun Lee;Ki Eun Lee;In Tae Cha;Won Jae Chi;Dong-Uk Kim
    • Korean Journal of Environmental Biology
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    • v.41 no.3
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    • pp.215-222
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    • 2023
  • In 2022, research for native prokaryotic species in Korea reported 10 unrecorded bacterial strains affiliated to phyla Actinomycetota, Bacillota, and Pseudomonadota. The strains formed monophyletic clades with the most closely related species (with ≥98.7% sequence similarity) in the 16S rRNA gene sequencing. Among them, four species of the phylum Actinomycetota, two species of the phylum Bacillota, and four species of the phylum Pseudomonadota have not been reported in Korea, suggesting unrecorded species in Korea. Information on strains such as Gram staining reaction, colony and cell morphology, biochemical characteristics, and isolation sources were provided in the species description.

Growth Performance and Meat Quality of Broiler Chickens Supplemented with Bacillus licheniformis in Drinking Water

  • Liu, Xiaolu;Yan, Hai;Lv, Le;Xu, Qianqian;Yin, Chunhua;Zhang, Keyi;Wang, Pei;Hu, Jiye
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.5
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    • pp.682-689
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    • 2012
  • A feeding trial was conducted to investigate effects of Bacillus licheniformis on growth performance and meat quality of broilers. Nine hundred one-d-old broiler chicks were randomly assigned to 3 experimental groups with three replicate pens of 100 broiler chicks. Three treatments were i) control, ii) basal diets supplemented with 1 ml of B. licheniformis for each in feed water per day iii) basal diets supplemented with 2 ml of B. licheniformis per chick in feed water per day. The supplementation of B. licheniformis significantly increased body weight in grower chickens (p<0.05), and significantly improved the feed conversion in 3 to 6 and 0 to 6 wk feeding period compared with the control group (p<0.05). Additionally, the supplement also resulted in increased protein and free amino acid contents, and decreased fat content in chicken breast fillet (p<0.05). Furthermore, improvement in sensory attributes was observed in broilers fed with the probiotic. In conclusion, B. licheniformis treatments resulted in a significant increase (p<0.05) in broiler productivity based on an index taking into account daily weight gain and feed conversion rate. Meanwhile, the probiotic contributed towards an improvement of the chemical, nutritional and sensorial characteristics of breast fillet. Overall, the study indicates that B. licheniformis can be used as a growth promoter and meat quality enhancer in broiler poultry.