• Title/Summary/Keyword: Biological Resource

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A New Record of Neosartorya aureola Isolated from Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.43 no.3
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    • pp.191-195
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    • 2015
  • A new species of Neosartorya was recovered during investigation of the fungal community in soil samples collected from different locations in Korea; Neosartorya aureola KNU14-7 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region of rDNA and morphological characteristics. The species has not been officially reported from Korea and we report it here with description and figures.

Complete genome sequence of Cohnella sp. HS21 isolated from Korean fir (Abies koreana) rhizospheric soil (구상나무 근권 토양으로부터 분리된 Cohnella sp. HS21의 전체 게놈 서열)

  • Jiang, Lingmin;Kang, Se Won;Kim, Song-Gun;Jeong, Jae Cheol;Kim, Cha Young;Kim, Dae-Hyuk;Kim, Suk Weon;Lee, Jiyoung
    • Korean Journal of Microbiology
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    • v.55 no.2
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    • pp.171-173
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    • 2019
  • The genus Cohnella, which belongs to the family Paenibacillaceae, inhabits a wide range of environmental niches. Here, we report the complete genome sequence of Cohnella sp. HS21, which was isolated from the rhizospheric soil of Korean fir (Abies koreana) on the top of Halla Mountain in the Republic of Korea. Strain HS21 features a 7,059,027 bp circular chromosome with 44.8% GC-content. Its genome contains 5,939 protein-coding genes, 78 transfer RNA (tRNA) genes, 27 ribosomal RNA (rRNA) genes, 4 noncoding RNA genes (ncRNA), and 90 pseudogenes. The bacterium contains antibiotic-related gene clusters and genes encoding plant cell wall-degrading enzymes.

Bacterial Community and Diversity from the Watermelon Cultivated Soils through Next Generation Sequencing Approach

  • Adhikari, Mahesh;Kim, Sang Woo;Kim, Hyun Seung;Kim, Ki Young;Park, Hyo Bin;Kim, Ki Jung;Lee, Youn Su
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.521-532
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    • 2021
  • Knowledge and better understanding of functions of the microbial community are pivotal for crop management. This study was conducted to study bacterial structures including Acidovorax species community structures and diversity from the watermelon cultivated soils in different regions of South Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities including Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genomic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained. NGS data results revealed that, most dominant phylum in all the soil samples was Proteobacteria (37.3%). In addition, most abundant genus was Acidobacterium (1.8%) in all the samples. In order to analyze species diversity among the collected soil samples, OTUs, community diversity, and Shannon index were measured. Shannon (9.297) and inverse Simpson (0.996) were found to have the highest diversity scores in the greenhouse soil sample of Gyeonggi-do province (GG4). Results from NGS sequencing suggest that, most of the soil samples consists of similar trend of bacterial community and diversity. Environmental factors play a key role in shaping the bacterial community and diversity. In order to address this statement, further correlation analysis between soil physical and chemical parameters with dominant bacterial community will be carried out to observe their interactions.

Physical Geographical Background of Geotourism Resources in Gumho River Basin (금호분지 내 지리관광자원의 자연지리학적 배경)

  • Son, Myoung-Won
    • Journal of the Korean association of regional geographers
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    • v.12 no.2
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    • pp.202-214
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    • 2006
  • In this paper Gumho drainage basin is divided into six geomorphic units (Bohyeon, Palgong, Biseul mountainous areas and Yeongcheon, Gyeongsan, Daegu basin), and physical geographical background and distributional characteristics of geotourism resources in each geomorphic unit are examined. Most(32 sites, 78%) of geotourism resources in Gumho basin reflect geomorphological environment, rest(7 sites, 17%) of them reflect geological environment. There are three geomorphological resources in Bohyeon mountainous areas, eight geomorphological resources in Palgong mountainous areas, and five geomorphological resources and one cultural resource in Biseul mountainous areas. There are two geomorphological resources, one geological resource, one biological resource, and two cultural resources in Yeongcheon basin. Among them one biological resource and and one cultural resource reflect local geomorphological environment. In Gyeongsan basin are there one geomorphological resource, three geological resources, and one biological resource, but one biological resource reflects local geomorphological environment. There are eight geomorphological resources, two geological resources, and three biological resources in Daegu basin. But two biological resources among them reflect local geomorphological environment.

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Two newly recorded echinoderms from the mesophotic zone in Korea

  • Michael Dadole Ubagan;Jinho Lee;Sook Shin;Taekjun Lee
    • Journal of Species Research
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    • v.12 no.2
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    • pp.180-188
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    • 2023
  • The region of the marine environment between shallow waters and deep-sea (30-150 m in depth) is referred to as the twilight or mesophotic zone. This zone has been scarcely examined, as these depths are too shallow for safe submersible operation. Since 2018, a survey of mesophotic echinoderms in Korea has yielded many specimens of interest. In this study, we present two newly recorded echinoderms, Henricia irregularis and Parastichopus nigripunctatus based on morphological redescriptions with high-definition photographs and DNA barcoding data for P. nigripunctatus.

Sphingomonas abietis sp. nov., an Endophytic Bacterium Isolated from Korean Fir

  • Lingmin Jiang;Hanna Choe;Yuxin Peng;Doeun Jeon;Donghyun Cho;Yue Jiang;Ju Huck Lee;Cha Young Kim;Jiyoung Lee
    • Journal of Microbiology and Biotechnology
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    • v.33 no.10
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    • pp.1292-1298
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    • 2023
  • PAMB 00755T, a bacterial strain, was isolated from Korean fir leaves. The strain exhibits yellow colonies and consists of Gram-negative, non-motile, short rods or ovoid-shaped cells. It displays optimal growth conditions at 20℃, 0% NaCl, and pH 6.0. Results of 16S rRNA gene-based phylogenetic analyses showed that strain PAMB 00755T was most closely related to Sphingomonas chungangi MAH-6T (97.7%) and Sphingomonas polyaromaticivorans B2-7T (97.4%), and ≤96.5% sequence similarity to other members of the genus Sphingomonas. The values of average nucleotide identity (79.9-81.3%), average amino acid identity (73.3-75.9%), and digital DNA-DNA hybridization (73.3-75.9%) were significantly lower than the threshold values for species boundaries; these overall genome-related indexes (OGRI) analyses indicated that the strain represents a novel species. Genomic analysis revealed that the strain has a 4.4-Mbp genome encoding 4,083 functional genes, while the DNA G+C content of the whole genome is 66.1%. The genome of strain PAMB 00755T showed a putative carotenoid biosynthetic cluster responsible for its antioxidant activity. The respiratory quinone was identified as ubiquinone 10 (Q-10), while the major fatty acids in the profile were identified as C18:1ω7c and/or C18:1ω6c (summed feature 8). The major polar lipids of strain PAMB 00755T were diphosphatidylglycerol, phosphatidylethanolamine, sphingoglycolipid, and phosphatidylcholine. Based on a comprehensive analysis of genomic, phenotypic, and chemotaxonomic characteristics, we proposed the name Sphingomonas abietis sp. nov. for this novel species, with PAMB 00755T as the type strain (= KCTC 92781T = GDMCC 1.3779T).

Characterizations of Broad Benn Wilt Virus isolated from Rehmania glutinosa Liboschitz

  • Park, In-Sook;Park, Min-Kyoung;Kim, Jong-Yeop;Lim, Ju-Rak;Lee, Kui-Jae;Lee, Wang-Hyu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11b
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    • pp.54-54
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    • 2002
  • The nucleotide sequence of a Korea isolate of broad bean wilt favavirus from Rehmannia glutinosa Lib., designated BBWV-RE, was determined. Direct amino acid sequencing of the virus coat proteins suggests that a comparison of several favaviruses in terms of nucleotide sequence and amino acid sequences showed that BBWV-2 isolates display high sequence identity. The small coat protein genes of RNA-2 were also determined for three other Japanese isolates(E, L, and 1-2) and two ATCC isolates(PV132 and PV176) of BBWV. the CP sequence suggested distinct evolution lineages. Serotype 2 favaviruses are more prevalent in Asia, Australia and North America, Wheres serotype 1 is more prevalent in europe.(중략)

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