• Title/Summary/Keyword: Biological Resource

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Phialocephala lagerbergii: A New Record from Crop Field Soil in Korea

  • Adhikari, Mahesh;Kim, Sangwoo;Yadav, Dil Raj;Um, Yong Hyun;Kim, Hyung Seung;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.132-137
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    • 2016
  • A unrecorded hyphomycete species of Phialocephala was isolated for the first time during the investigation of fungal community in the soil samples collected from different regions of Korea. The fungal isolate was identified as Phialocephala lagerbergii, based on the morphological characteristics and phylogenetic analysis of the ribosomal DNA sequence. In addition, cultural and micro-morphological features were described in detail.

Arthrobacter subterraneus sp. nov., Isolated from Deep Subsurface Water of the South Coast of Korea

  • Chang, Ho-Won;Bae, Jin-Woo;Nam, Young-Do;Kwon, Hyuk-Yong;Park, Ja-Ryeong;Shin, Kee-Sun;Kim, Kyoung-Ho;Quan, Zhe-Xue;Rhee, Sung-Keun;An, Kwang-Guk;Park, Yong-Ha
    • Journal of Microbiology and Biotechnology
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    • v.17 no.11
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    • pp.1875-1879
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    • 2007
  • Strain $CH7^T$, a pale yellow-pigmented bacterium and new isolate from deep subsurface water of the South Coast of Korea, was subjected to a polyphasic taxonomic study. $CH7^T$ grew between 5 and $37^{\circ}C$, pH 5.3-10.5, and tolerated up to 13% NaCl. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain $CH7^T$ was associated with the genus Arthrobacter and phylogenetically closely related to the type strains Arthrobacter tumbae (99.4%) and Arthrobacter parietis (99.1%). However, DNA-DNA hybridization experiments revealed 2.1% and 12% between strain $CH7^T$ and Arthrobacter tumbae and Arthrobacter parietis, respectively. Thus, the phenotypic and phylogenetic differences suggested that $CH7^T$ should be placed in the genus Arthrobacter as a novel species, for which the name Arthrobacter subterraneus sp. novo is proposed. In addition, the type strain for the new species is $CH7^T$ ($=KCTC\;9997^T=DSM\;17585^T$).

Isolation and Characterization of Fungal Diversity from Crop Field Soils of Nigeria

  • Yadav, Dil Raj;Kim, Sang Woo;Adhikari, Mahesh;Babu, Anam Giridhar;Um, Yong Hyun;Gim, Eun Bi;Yang, Jae Seok;Lee, Hyug Goo;Lee, Youn Su
    • 한국균학회소식:학술대회논문집
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    • 2014.10a
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    • pp.49-49
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    • 2014
  • In order to find indigenous beneficial fungal species from crop field soils of Nigeria, 23 soil samples were collected from various places of Nigeria in June, 2013 and fungi were isolated through serial dilution technique. Isolated fungi were purified and differentiated according to their morphological and microscopic characteristics. In total, 38 different representative isolates were recovered and the genomic DNA of each isolates was extracted using QIAGEN$^{(R)}$ Plasmid Mini Kit (QIAGEN Sciences, USA) and the identification of fungi was carried out by sequence analysis of internal transcribed spacer (ITS) region of the 18S ribosomal DNA (18S rDNA). Recovered isolates belonged to 9 fungal genera comprising Fusarium, Aspergillus, Chaetomium, Coniothyrium, Dipodascaceae, Myrothecium, Neosartorya, Penicillium and Trichoderma. Aspergillus spp., Penicillium spp. and Trichoderma spp. were the most dominant taxa in this study. The antagonistic potentiality of species belonged to Trichoderma against 10 phytopathogenic fungi (F. oxysporum, C. gloesporoides, P. cytrophthora, A. alternata, A. solani, S. rolfsii, F. solani, R. solani, S. sclerotiorum and P. nicotiana) was assessed in vitro using dual culture assay. The dual culture assay results showed varied degree of antagonism against the tested phytopathogens. The potential Trichoderma spp. will be further evaluated for their antagonistic and plant growth promotion potentiality under in vivo conditions.

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Three New Records of Mortierella Species Isolated from Crop Field Soil in Korea

  • Yadav, Dil Raj;Kim, Sang Woo;Adhikari, Mahesh;Um, Yong Hyun;Kim, Hyun Seung;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • v.43 no.3
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    • pp.203-209
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    • 2015
  • Three new fungal species of the genus Mortierella, Mortierella zychae, Mortierella ambigua, and Mortierella indohii, have been reported in Korea. The fungi were encountered during a study on the fungal community of soil samples collected from different locations in Korea. The species were identified based on molecular and morphological analyses. This study presents detailed descriptions of the morphological observations and molecular phylogenetic analysis of these three fungi. All three species were found to be sensitive to triphenyltetrazolium chloride staining. M. zychae demonstrated the highest intensity of mycelial staining, indicating that this species has the highest potential to produce arachidonic acid of the three species. The staining results indicated that the newly recorded species could potentially be useful for arachidonic acid production.

An Introduction of Management and Policy of Biological Resources (생물자원의 관리와 정책)

  • Cho, Soon-Ro;Seol, Sung-Soo;Park, Jung-Min
    • Journal of Korea Technology Innovation Society
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    • v.11 no.2
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    • pp.219-240
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    • 2008
  • This paper aims to suggest a policy for biological resource based on a comprehensive understanding on biological resources. Biological resources are different from traditionally recognized viable organisms (Biodiversity) in ecosystems. Biological resources are culturable and replicable resources of living organisms such as tissues, cells and genes. Moreover, biological resources include human-derived biological materials. Biological resources is not simply a matter of science and technology. Biological resources should be dealt with as national resources. There are many international issues regarding biological resources, such as intellectual property rights (IPRs), safety on handling and distribution, material transfer agreements (MTAs) for mutual benefits and biological standards. Ethical debates are also being raised because biological resources are related with human-derived biological materials. Every nation has tendency to adopt its government policies to strengthen its sovereignty on biological resources and international cooperation. In addition, international linkages are essential for providing enhanced worldwide accessibility to biological resources. Japan has shown several international initiatives in the field of biological resources. Korea has just begun to design appropriate policies for the use and R&D of biological resources. Therefore, this paper suggests the following needs: 1) policy at the national level beyond the interests of researchers, 2) inter-ministerial coordination across government ministries, 3) expansion of scope and size of each BRC (Biological Resource Centers), and 4) building networks and systems such as national information center, representative centers by field, and each BRC.

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Westerdykella reniformis: A New Record from Field Soils in Korea

  • Adhikari, Mahesh;Kim, Sang Woo;Gwon, Byeong Heon;Ju, Han Jun;Lee, Hyang Burm;Lee, Youn Su
    • The Korean Journal of Mycology
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    • v.48 no.1
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    • pp.47-53
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    • 2020
  • During a survey of fungal diversity in different provinces of South Korea in 2017, a new fungal isolate was discovered. This fungal isolate was identified as Westerdykella reniformis, based on its morphological characteristics and phylogenetic analysis, using internal transcribed spacer (ITS) and 28S ribosomal DNA (28S rDNA) sequence data. To our knowledge, W. reniformis has not previously been reported in South Korea. Thus, in this study, we report a new record of a species from the Dothideomycetes class in Korea, and provide a detailed description with morphological illustrations.

The Influence of Light Reduction on the Growth of Microcystis aeruginosa and Variation of Environmental and Chemical Parameters in Large-scale Cultivation System

  • Yang, Taehui;Cho, Ja-young;Kang, Ha-jin;Lee, Chang Soo;Kim, Eui-jin
    • Korean Journal of Ecology and Environment
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    • v.53 no.4
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    • pp.336-343
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    • 2020
  • Large-scale cultivation of Microcystis aeruginosa in different light conditions was conducted for verifying the cell growth in a greenhouse system. Environmental and chemical parameters of the large-scale culture medium were measured for analyzing the interaction between M. aeruginosa and its symbiotic bacteria. During cultivation, a difference in cell growth pattern was observed between control (natural light) and light-limited groups (reduction of blue, green, and blue/green light, respectively). Comparing the control group, the light reduced groups showed slow and delayed cell growth through the cultivation period. Also, there is differences in the consuming pattern of total nitrogen and total phosphorus which indicated that the possibility of interaction between M. aeruginosa and symbiotic bacteria.

Diversity of Fungi Isolated from Soil of Jeollabuk-do and Chungcheongbuk-do, Korea

  • Yadav, Dil Raj;Adhikari, Mahesh;Kim, Sang Woo;Kim, Hyun Seung;Um, Yong Hyun;Lee, Youn Su
    • 한국균학회소식:학술대회논문집
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    • 2015.11a
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    • pp.30-30
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    • 2015
  • This study was conducted aiming with the assessment of fungal diversity in soil samples collected from different locations of Jeollabuk-do and Chungcheongbuk-do, Korea. Forty soil samples were collected in 2015 and fungi were isolated through serial dilution technique. Isolated fungi were purified and differentiated according to their morphological and microscopic characteristics. In total, 150 different representative isolates were recovered and the genomic DNA of each isolate was extracted by using QIAGEN$^{(R)}$ Plasmid Mini Kit (QIAGEN Sciences, USA) and the identification of fungi was carried out by sequence analysis of internal transcribed spacer (ITS) region of the 18S ribosomal DNA (18S rDNA). Recovered isolates belonged to 37 family, 67 genera and 108 species. Aspergillus spp., Penicillium spp., Trichoderma spp., Chaetomium spp. And Fusarium spp. were the most dominant taxa in this study. Out of total species, 20 species were identified as new records for Korea.

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Pathogenesis of Oak Wilt Disease Caused by Raffaelea Species

  • Kim, Sang Woo;Yadav, Dil Raj;Adhikari, Mahesh;Um, Yong Hyun;Kim, Hyun Seung;Lee, Youn Su
    • 한국균학회소식:학술대회논문집
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    • 2015.11a
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    • pp.18-18
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    • 2015
  • Wilt disease in Oak trees occurs during summer season in Korea. Mass attack of trees by an ambrosia beetle (Platypus koryoensis) was the characteristic feature before appearance of the wilting symptoms. Raffaelea sp. caused the discoloration of xylem area called as wound heartwood. Raffaelea sp. was observed both on the body surfaces and inside the mycangia of the beetle Platypus sp. The scanning electron microscope (SEM) analysis showed that fungal spores were present within the wall of gallery and vessels that formed tyloses. The results revealed that the water movement in vessels was blocked as the fungus started to grow which caused the formation of tyloses thereby resulting wilt symptoms. We found that both female and male beetle Platypus sp. had fungi on their bodies and their large and small mycangia. This study confirmed that the fungus was transferred to oak trees by Platypus sp.

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