• Journal of Environmental Science International
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• v.12 no.4
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• pp.427-437
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• 2003

An evaluation of the application of SBR and biofilm en small sewerage system was conducted. A newly developed small sewerage system, using SBR, was successfully applied to the nutrient treatment using municipal wastewater. The system was consisted of 6 compartments. Two systems, with SBR (A type) or without SBR (B type), were compared by several parameters (COD, SS, T-N, NH$_4$$\^$+/-N, NO$_3$$\^$-/-N, NO$_2$$\^$ -/-N, alkalinity, pH, DO) in all experimental periods. Also, the time variation of several parameters (DO, pH, NH$_4$$\^$+/-N, NO$_3$$\^$-/-N NO$_2$$\^$-/-N) was examined in a SBR applied sewerage system. T-N removal efficiency of B type Was higher than that Of A type by the effect of nitrification and denitrification even though the COD removal efficiencies were similar. In aeration stage, the pH was decreased from 6.4 to 6.3 within 1 h and increased to 6.65 at the end of aerobic stage, and pH was decreased to 6.2 in non-aeration stage, and these phenomena were explained. The effects of nitrification and denitrification were compared in A type and B type sewerage system, and the typical nitrification and denitrification were observed in B type sewerage system.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.23-26
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• 2004

Methyl Tertiary-Butyl Ether is one of several fuel oxygenates added to gasoline to improve fuel combustion and reduce tile resulting concentration of hydrocarbon. Thus, MTBE transfer readily to groundwater from gasoline leaking from Underground Storage Tank. Therefor, there are significant risks and costs associated with the water contamination. MTBE is far more water soluble than gasoline hydrocarbon. The purpose of the this study is to test the ability of ground tire with facultative bacteria. Bacillus brevis, to sorb MTBE. The process is consisted both batch and column experiment to determine the sorption capacity. And Biofilm is observed by SEM in the column. Finally, it is clear that ground tire represent an attractive and relatively inexpensive sorption medium for a MTBE. The authors can surmise that to determine the economic cost of ground tire utilization, tile cost to sorb a given mass of contaminant by ground tire will have to be compared to currently accepted sorption media. and Bacillus brevis strain was eliminated on MTBE, too. The biobarrier that ground tire with bacteria, has potential for use in the remediation of MTBE-contaminated environments.

• Journal of Korean Society of Water and Wastewater
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• v.12 no.2
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• pp.83-89
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• 1998

The drinking water supply in Cheju entirely depends on the ground water and recently the polluted ground water bores are increasing. In 1993 Cheju Province Health-Environment Institute reported that the ground water quality of 26 of 98 bores under the drinking water quality standard. Therefore there are many investigation in the needs of the nitrate removal in the drinking water in the regin with no alternative water resources. In this study, the following results are obtained to remove the nitrate in biofilm filtration process in which uses ethanol as external carbon source. Over 90% of nitrate is removed after 10 days of experiment. The nitrate removal rate on filtration velocity is about 100% at 50m/day and 100m/day, and about 56% at 200m/day. The removal rate is reduced in 27% at 400m/day. Using ethanol as the external carbon source, denitrification kinetic is 1st-order. Denitrification constant k is 8.004($hr^{-1}$). The amount of the denitrificated-Nitrogen is increased as the contact time increased. Deoxydation rate constant ${\gamma}$ is 11.895($hr^{-1}$). 0.968g of ethanol(as TOC) is needed to remove 1g of nitrate and 0.291g is required to remove 1g of dissolved oxygen.

• Environmental Engineering Research
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• v.23 no.2
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• pp.136-142
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• 2018

The river water source of Saidabad Surface Water Treatment Plant at Dhaka, Bangladesh, is deteriorated too much to be treated by conventional treatment process due to excessive ammonia pollution. In order to improve the raw water quality before it enters into the main treatment chain, a pilot study was conducted for pre-treatment of the raw water. The objective is to investigate the rate of reduction of ammonia using the Meteor pilot, a biological pretreatment system, which is a laboratory scale Moving Bed Biofilm Reactor with a nominal volume of hundred liters, filled with 50 L of Meteor 660 media. The reduction of ammonia was quite significant on average 73%, while the reduction of COD was in a range from 20 to 60%. The Meteor pilot was able to treat and nitrify the raw water and produce an effluent that respects the guarantee of ammonia < $4.0mg\;NH_3-N/L$ when the raw water ammonia concentration was < $15mg\;NH_3-N/L$. The study identified operating parameters necessary to achieve the desired goal of adequate ammonia removal. The study results would benefit a range of systems across the country by providing guidance on the design and operation of a biological pre-treatment system for ammonia removal.

• Environmental Engineering Research
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• v.22 no.2
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• pp.210-215
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• 2017

Produced water is the largest wastestream of oil and gas exploration. It consists of various organic and inorganic compounds that hinder its beneficial use. This study compared the treatment of produced water in a batch suspended and biofilm activated sludge process. The biofilm carrier material was made from Gardenia Carinata shell. COD, $NH_4{^+}-N$ and $NO_3-N$ removal was monitored in both the suspended (control) and floating carrier bioreactors. The results show a rapid reduction of produced water constituents in the floating carrier bioreactor. COD, $NH_4{^+}-N$ and $NO_3-N$ removal was in the range of 99%, 98% and 97% for the floating carrier bioreactor whereas it was 88%, 84% and 83% for the control bioreactor. The rapid reduction of COD, $NH_4{^+}-N$ and $NO_3-N$ clearly indicate that the floating carrier materials served as an attached growth medium for microorganisms, improved the breakdown of produced water constituents and reduced inhibition of microbial metabolic activities.

• Environmental Engineering Research
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• v.10 no.6
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• pp.283-293
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• 2005

Characteristics of sulfur-based autotrophic denitrification in a semi-continuous type reactor and the kinetic parameters were studied. Enriched autotrophic denitrifying culture was used for the reactor operation. Biomass growth on sulfur particles and in the liquid medium was monitored using the DAPI staining method. From the result of ion concentration changes and the biomass growth, maximum specific growth rate, ${\mu}_{max}$, and the half velocity constant, $K_M$, were estimated as $0.61\;d^{-1}$ and 3.66 mg/L, respectively. Growth yield coefficient, Y values for electron acceptor and donor were found as 0.49 gVSS/g N and 0.16 gVSS/g S. The biomass showed specific denitrification rate, ranging 0.86-1.13 gN/g VSS-d. A half-order equation was found to best simulate the denitrification process in the packed bed reactor operated in the semi-continuous mode.

• Journal of Korean Society on Water Environment
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• v.38 no.4
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• pp.171-176
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• 2022

Recently, an autotrophic single-stage nitrogen removal (ASSNR) process based on the anaerobic ammonium oxidation (ANAMMOX) reaction has been proven as an economical ammonia treatment. It is highly evident that double-layered gel beads are a promising alternative to the natural biofilm for ASSNR because of the high mechanical strength of poly(vinyl alcohol) (PVA)/alginate structure and efficient protection of ANAMMOX bacteria from dissolved oxygen (DO) due to the thick outer layer. However, the thick outer layer results in severe mass transport limitation and consequent lowered bacterial activity. Therefore, the effects of the thickness of the outer layer on the overall reaction rate were tested in the biofilm model using AQUASIM for ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and ANAMMOX bacteria. A thickness of 0.5~1.0 mm is preferred for the maximum total nitrogen (TN) removal. In addition, a DO of 0.5 mg/L resulted in the best total nitrogen removal. A higher DO induces NOB activity and consequent lower TN removal efficiency. The optimal density of AO B and NO B density was 1~10% for a 10% ANAMMOX bacterial in the double-layered PVA/alginate gel beads. The real effects of operating parameters of the thickness of the outer layer, DO and concentrations of biomass balance should be intensively investigated in the controlled experiments in batch and continuous modes.

• Environmental engineer
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• s.170
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• pp.56-62
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• 2000

현재 국내의 오폐수처리장의 수는 증가하고 있으며 그에 따른 오폐수처리장의 건설비는 물론 운전관리비용도 증가하는 추세이다. 이에 따라 우수한 처리효율과 경제적이고 안정성을 갖춘 공정이 시급히 요구되고 있다. 1998년 12월에서 2001년 11월까지 3년간 수행될 생물막공법을 이용한 소규모 오수처리시설에서의 유기물 및 영양염 제거공정의 최적화 및 상용화에 대한 본 연구에서, 1차년도 목표인 TN 10mg/l, TP 1mg/l 이하의 안정적인 유출수질을 얻

• Korean Journal of Microbiology
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• v.50 no.2
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• pp.128-136
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• 2014

Acyl homoserine lactone (AHL) lactonase has been proved to be the AHL-degrading enzyme with the highest substrate specificity for AHL molecules and has shown a considerable potential as low-cost and efficient quorum quenching (QQ) technique. However, few studies focused on its inhibitory effect on biofilm formation which is also a quorum sensing (QS)-regulated phenomenon. In this study, QQ activity of six isolates from biofouled reverse osmosis membranes was studied using Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NTL4 as biosensors under various conditions. All of the isolates belonged to the genus Bacillus and showed QQ activity regardless of the acyl chain length or substitution of AHL molecule. The isolates were capable of significantly inhibiting biofilm formation (46.7-58.3%) by Pseudomonas aeruginosa PAO1 and produced heat-sensitive extracellular QQ substances. The LC-MS analysis of the QQ activity of a selected isolate, RO1S-5, revealed the degradation of N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12 AHL) and the production of corresponding acyl homoserine (3-oxo-C12-HS), which indicated the activity of AHL lactonase. The broad AHL substrate range and high substrate specificity suggested that the isolate would be useful for the control of biofilm-related pathogenesis and biofouling in industrial processes.

• Fisheries and Aquatic Sciences
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• v.1 no.2
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• pp.242-249
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• 1998

The engineering aspect of water treatment processes in the recirculating aquaculture system was studied. To recycle the water in the aquaculture system, a wastewater treatment process was required to maintain high water quality for the growth and health of the cultured fish. In this study, three different biofilm processes were used to reduce the concentration of organic matters and ammonia from the recirculating water - two phase fluidized bed, three phase fluidized bed, and trickling filter. The objectives of this research were to evaluate the optimum treatment conditions of the biofilm processes for the recirculating aquaculture system, and thereby reduce the volume of biofilm processes, which are commonly used for the recycle water treatment processes for aquaculture. The result of this study showed that the removal efficiency of organic matters by trickling filter was found to be lower than that of the fluidized bed. In the trickling filter system, anthracite showed better organic removal efficiency than crushed stone as a media. In the two phase fluidized bed, the maximum removal efficiency of either organics or ammonia was obtained when both the packing rate of media was maintained to $40\%$ of total reactor depth excepting sediment zone and the bed expansion rate was maintained to $100\%$. When 100 tilapia (Oreochromis niloticus) of each average 200g was reared, the pollutant production rate was 0.07g $NH_4\;^+-N/kg$ fish/day and 0.06g P04-3-P/kg fish/day, and sludge production rate was 0.39 g SS/kg fish/day. In the two phase and three phase fluidized bed, the volume of water treatment tank could be calculated from an empirical equation by using the relationship between the influent COD to $NH_4\;^+-N$ ratio (C/N, -), media concentration (Cm, g/L), influent ammonia nitrogen concentration (Ni, mg/L), effluent ammonia nitrogen concentration (Ne, mg/L), bed expansion rate $(E,\;\%)$, and influent flowrate $(Q,\;m^3/hr)$. The empirical equation from this study is $$V_2\;=\;10^{3.1279}\;C/N^{3.5461}\;C_m\;^{-3.7473}\;N_i\;^{4.6477}\;E^{0.0326}\;N_e\;^{-0..8849}\;Q\;(Two\;Phase\;FB) V_3\;=\;10^{11.7507}\;C/N^{-1.2330}\;C_m\;^{-6.5715}\;N_i\;^{1.5091}\;N_e\;^{-1.8489}\;Q (Three\;Phase\;FB)$$