• The Journal of Advanced Prosthodontics
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• v.5 no.2
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• pp.140-146
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• 2013

PURPOSE. The aim of this study was to investigate the destructive effects of biofilm formation and/or biocorrosive activity of 6 different oral microorganisms. MATERIALS AND METHODS. Three different heat polymerized acrylic resins (Ivocap Plus, Lucitone 550, QC 20) were used to prepare three different types of samples. Type "A" samples with "V" type notch was used to measure the fracture strength, "B" type to evaluate the surfaces with scanning electron microscopy and "C" type for quantitative biofilm assay. Development and calculation of biofilm covered surfaces on denture base materials were accomplished by SEM and quantitative biofilm assay. According to normality assumptions ANOVA or Kruskal-Wallis was selected for statistical analysis (${\alpha}$=0.05). RESULTS. Significant differences were obtained among the adhesion potential of 6 different microorganisms and there were significant differences among their adhesion onto 3 different denture base materials. Compared to the control groups after contamination with the microorganisms, the three point bending test values of denture base materials decreased significantly (P<.05); microorganisms diffused at least 52% of the denture base surface. The highest median quantitative biofilm value within all the denture base materials was obtained with P. aeruginosa on Lucitone 550. The type of denture base material did not alter the diffusion potential of the microorganisms significantly (P>.05). CONCLUSION. All the tested microorganisms had destructive effect over the structure and composition of the denture base materials.

• Journal of Microbiology and Biotechnology
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• v.24 no.2
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• pp.280-286
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• 2014

Four thermophilic bacterial species, including the iron-reducing bacterium Geobacillus sp. G2 and the sulfate-reducing bacterium Desulfotomaculum sp. SRB-M, were employed to integrate a bacterial consortium. A second consortium was integrated with the same bacteria, except for Geobacillus sp. G2. Carbon steel coupons were subjected to batch cultures of both consortia. The corrosion induced by the complete consortium was 10 times higher than that induced by the second consortium, and the ferrous ion concentration was consistently higher in iron-reducing consortia. Scanning electronic microscopy analysis of the carbon steel surface showed mineral films colonized by bacteria. The complete consortium caused profuse fracturing of the mineral film, whereas the non-iron-reducing consortium did not generate fractures. These data show that the iron-reducing activity of Geobacillus sp. G2 promotes fracturing of mineral films, thereby increasing steel corrosion.

• Microbiology and Biotechnology Letters
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• v.30 no.1
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• pp.86-90
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• 2002

Cooling water sampled at Pohang Steel Company, Korea, was used to study the effect of biocide (NaOCl) on biofilm formation and metal corrosion. Planktonic microorganisms were killed in the presence of biocide (0.2％ NaOCl) within 1.5 h, but not sessile microorganisms in biofilms even after one week. Black color of biofilms, possibly due to the activity of sulfate reducing bacteria, were made with the natural cooling waters, while orange color of biofilms were formed when cooling waters were autoclaved or when 0.2％ NaOCl was added to the natural cooling waters. Microbially influenced corrosion rate in black color of biofilms was 2.3 fold higher than that in orange color of biofilms.