• Title/Summary/Keyword: Biochemical substances

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Effects of Light Quality of a Light-Emitting Diode (LED) on Carbohydrate, Protein, and Lipid Contents of Tetraselmis suecica and T. tetrathele (발광다이오드(LED) 파장에 따른 Tetraselmis suecica와 T. tetrathele의 탄수화물, 단백질 및 지질 함량에 미치는 영향)

  • Kyong Ha Han;Seok Jin Oh
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.29 no.1
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    • pp.36-43
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    • 2023
  • To establish a culture system with enhanced cellular nutrition, we investigated the effects of light quality (blue, 450 nm; yellow, 590 nm; and red, 630 nm) of a light-emitting diode (LED) on the biochemical composition of Tetraselmis suecica and T. tetrathele. The protein content of both species was higher (42-69%) than the content of other biochemical substances under all wavelengths. Carbohydrate, protein, and lipid contents were higher under the yellow wavelength, which showed a low growth rate, than those under other wavelengths. The contents of all biochemical substances were low under the red wavelength, which showed a high growth rate. These results indicated that protein synthesis occurs in response to decreased cell division rate, while lipid and carbohydrate synthesis occurs owing to altered chemical composition and enzymatic activity. Therefore, we suggested a two-phase LED culture system, which emitted red LED during the early-middle exponential phase and yellow LED during the late exponential and stationary phases, to increase the yield of useful biochemical substances of T. suecica and T. tetrathele.

On the Forming Processes of Soil Humic Substances and its Physiological Effects on Plants (토양(土壤) 부식물(腐植物)의 생성(生成)과 효과(効果)에 대(對)한 고찰(考察))

  • Lim, Sun-Uk
    • Korean Journal of Soil Science and Fertilizer
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    • v.6 no.1
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    • pp.67-73
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    • 1973
  • Soil humic substances are defined as a humified part of the soil organic matters and regarded to play beneficial roles for colloid chemical properties and the fertility of the soils. This paper is referred to review the present trend of the studies on the forming processes of humic substances and on the effect on plant metabolism by some organic compounds that are directly absorbed by plants. It is generally considered that the humic substances are formed organic matters in soil or plant materials through numerous organic or biochemical processes. However, the nature of the constituting "core" and of attachment of carbohydrate, nitrogen containing compounds like protein, phenolic compounds and metals to the core are unclear though various models are suggested. It is reviewed that some organic compounds, phenclic acids, derived from humic substances are effective on plant metablism in many cases, although the mechanisms are remained to be clarified.

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Critical Review on Biochemical Characteristics of Kimchi(Korean Fermented Vegetable Products) (김치의 생화학적 특성)

  • 최홍식
    • Journal of the East Asian Society of Dietary Life
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    • v.5 no.2
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    • pp.89-101
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    • 1995
  • Kimchi is a fermented Korean vegetable product prepared using mafor raw materials (oriental cabbage and radish) and other ingredients through a series of processes of grading, brining, blending, and fermentation. Kimchi fermentation is initiated by various microorganisms originally present in the raw materials, but the fermentation is gradually dominated by lactic acid bacteria. Thus, the complex biochemical activities obviously occur during, before and after kimchi fermentation and their biochemical characteristics greatly differ, depending on the raw materials and processes used. This review covers in detail the numerous biochemical characteristics of sugars, organic acids, amino acids, vitamins(B complex, carotene and ascorbic acid), pectic substances, flavor components and others during preparation and preservation of kimchi.

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THE EFFECT OF BISPHENOL A ON THE THYROID HORMONE SYSTEM FOR THE ESTABLISHMENT OF SCREENING METHOD OF ENDOCRINE DISRUPTORS

  • Cho, Mi-Young;Lim, Myung-Sin;Lee, Young-Choi;Jung, Ki-Kyung;Nam, Kyung-Tak;Kim, Tae-Gyun;Kang, Ju-Hye;Kang, Seog-Youn;Kim, Seung-Hee
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2002.11b
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    • pp.162-162
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    • 2002
  • There has been many findings of natural, environmental or manufactered nonsteroidal substances shown to have estrogenic activity. Since estrogens affect reproduction and cellular development to cause disease in people or animals, chronic exposure may have a major impact on health.(omitted)

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Morphological and Biochemical Studies on the Effects of Cadmium on the Rat Testis (카드뮴이 고환에 미치는 영향에 관한 형태학적 및 생화학적 연구)

  • 권경석
    • Development and Reproduction
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    • v.1 no.2
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    • pp.125-132
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    • 1997
  • To investigate the cadmium (Cd) toxicity on the testis, male rats were treated with 1, 2, 4 and 8 mg/kg of Cd by IP. According to histochemical studies, Cd-treated testis tissue showed death of spermatozoa, death of Sertoli cells, death of all the spermatogenic cells, and finally disappearance of basal lamina of seminiferous tubules with increasing doses, and showed decreased ground substances and Leydig cells, increased inflammatory cells and fibroblasts, and fibroblasts, and finally disappearance of ground substances and all the cells except fibroblasts within interstitial tissues with increasing doses. According to biochemical studies, two kinds of proteins, 25 and 45 kDa, were dramatically disappeared from the total protein of rat testis treated with Cd comparing to normal testis. The result of electrophoresis of total protein suggests that actin (45 kDa), presumed on its mmolecular weight and amount, in the testis-cells is the primary target of Cd poisoning. Although its exact mechanism is not clear, the disappearance of two proteins when testis is exposed to Cd should give some clues to understnad the mechanism of necrosis of testis tissue crumbling by heavy metal pollutant such as Cd.

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A study on pearl millet (Pennisetum glaucum L.) plant Biochemical and histochemical changes inoculated with indigenous AM fungi under Barren soil

  • Pal, Ajay;Pandey, Sonali
    • Journal of Plant Biotechnology
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    • v.44 no.2
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    • pp.203-206
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    • 2017
  • The soil organisms that develop beneficial Symbiotic relationships with plants roots and contribute to plant growth are mycorrhizal (AM) fungi. Arbuscular mycorrhizal inoculations change the growth and biochemical composition of the host plant and soil. Mycorrhizal root systems do augment the absorbing area of roots from 10 to 100 times thereby greatly improving the ability of the plants to utilize the soil resources. A pot experiment was conducted during the kharif seasons at Jaipur, Rajasthan, to find out the effects of three different indigenous AM fungi i.e. Glomus mosseae, Glomus fasciculatum and Gigaspora decipiens either single and in combination inoculation on biochemical and histochemical changes of Pearl millet (Pennisetum glaucum L.) grown under barren soil conditions. The AM fungus has shown to improve the tolerance of plant to drought stress. Experimental results showed that AM fungi treated plants improved their plants growths, biochemical and histochemical changes as compared to non-mycorrhizal treatments. The AM fungi inoculated plant was found to be attaining maximum plant biochemical and histochemical substances in Glomus mosseae (alone) and also Glomus mosseae + Glomus fasciculatum treatments.

Comparison of Biochemical Components among Different Fodders-treated Antlers (각종 사료군의 투여에 따른 녹용 성분의 비교)

  • Ha, Young-Wan;Jeon, Byong-T.;Moon, Sang-H.;Kim, Yeong-Shik
    • Korean Journal of Pharmacognosy
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    • v.34 no.1 s.132
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    • pp.40-44
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    • 2003
  • Antler has been used as one of the important traditional oriental medicines for many years. It contains many biochemical components including lipids, peptides, carbohydrates, and inorganic substances. The various biological activities of antler are being considered owing to such biochemical components. The purpose of this research is to compare the biochemical components of antlers after treatment of three different kinds of fodder. They are mulberry (group A), Lycii Fructus (group B) and the complex of herbs (group C). The chemical composition of each antler was determined in three sections (top, middle, and bottom) and compared with those of the control. The contents of sialic acid, uronic acid and glycosaminoglycans increased in the top antlers of the group B. Total lipids content increased in the top antlers of all groups (A, B, and C). The concentration of inorganic ions (Ca, Mg, and P) decreased in all groups. Fattyacid composition was also analyzed by GC-MS and expressed as percentage of total fatty acid concentration. The content of palmitic acid decreased in all groups. The content of 2-hydrox-yhexadecanoic acid, which has not been reported in aster, increased in the top antlers of all groups. These results suggest that the treatment of special fodder may affect the composition of the biochemical components of antlers.

Molecular/biochemical Biomarkers for Exposure to Hazardous Chemicals in the Water Environment and their Application to Freshwater Fish (유해물질 노출로 인한 분자.생화학적 바이오마커와 담수 어류에 대한 현장 적용성)

  • Kim, Jung-Kon;Park, Ye-Na;Kim, Woo-Keun;Kim, Ji-Won;Lee, Sung-Kyu;Choi, Kyung-Ho
    • Journal of Environmental Health Sciences
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    • v.36 no.5
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    • pp.418-434
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    • 2010
  • As concerns regarding water pollution grow, the need increases for a fast and accurate assessment of ecological risk. In this context, many studies have been conducted to identify biomarkers which can sensitively indicate exposure to and effects of various contaminants in a water environment. However, the utility of most such biomarkers in the real water environment is not yet validated. In this paper, we conducted a thorough review of publications that were related to developing or evaluating molecular and biochemical biomarkers of freshwater fish in ecological risk assessment, and evaluated whether these biomarkers of interest could link to the effects on higher biological levels, such as histopathology and above. Biomarkers of interest included those associated with metabolism, oxidative stress, reproduction and endocrine disruption, genotoxicity, and defense against heavy metal exposure. We found that, when used alone, most molecular and biochemical biomarkers are not sufficient to understand the effects of toxic substances in higher biological levels, due to defense or acclimation mechanisms of organisms. Moreover, some biomarkers respond not only to hazardous substances but also to the changes in water quality and disease outbreak. Molecular and biochemical biomarkers may be most useful in understanding the potential biological effects of toxic compounds when used in parallel with relevant endpoints of higher biological levels.

Role of Exopolymeric Substances (EPS) in the Stability of the Biofilm of Thiomonas arsenivorans Grown on a Porous Mineral Support

  • Michel, Caroline;Garrido, Francis;Roche, Emilie;Belval, Sylvain Challan;Dictor, Marie-Christine
    • Journal of Microbiology and Biotechnology
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    • v.21 no.2
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    • pp.183-186
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    • 2011
  • Biochemical methods were selected to evaluate the role of exopolymeric substances in the stability of biofilms used in bioremediation processes. Biofilms of Thiomonas arsenivorans formed on pozzolana were thus treated with pronase (protein target), lectins (Con A or PNA), calcofluor or periodic acid (polysaccharides target), DNase (DNA target), and lipase (triglycerides target). Neither protease nor DNase treatments had any effect on bacterial adhesion. Lectins and calcofluor treatments mainly affected young biofilms. Lipase treatment had a noticeable effect on biofilm stability whatever the biofilm age. Results suggest that it would be an increased resistance of mature biofilms that protects them from external attacks.

토양길항세균 Bacillus sp. KL-3의 대사산물을 이용한 벼도열병균 Pyricularia oryzae의 생물학적방제

  • 김규영;김상달
    • Microbiology and Biotechnology Letters
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    • v.25 no.4
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    • pp.396-402
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    • 1997
  • Biocontrol of plant pathogens provides an alternative means of reducing the incidence of plant diseases without the negative aspects of chemical pesticides. Nowdays, as the resistant fungi about the chemical fungicides have revealed and the concern of environment has increased, the biological control of phytopathogenic fungi by the antagonistic microorganisms is very much indispensable. For the selection of strong antagonistic bacterium for biological control agent of rice leafblast and cucumber gray mold rot, the antifungal strain KL-3 strain was selected among 120 strains isolated from the rhizosphere soils. And the strain was identified to be a species of Bacillus subtilis or closely related strain. In several biochemical and in vitro antibiosis tests, antifungal substances of Bacillus sp. KL-3 were presumed heat stable, micromolecular antibiotic substances. In vivo test and vinyl house field test, the antifungal substances of Bacillus sp. KL-3 represented excellent biocontrol ability aganist Alternaria mali, Phyricularia oryzae, and Alternaria kikuchiana as well as broad spectrum of other fungi. In particular, Bacillus sp. KL-3 strain showed more predominant activity than some chemical fungicides against fungi shown to resist chemcal fungicides.

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