• Title, Summary, Keyword: Biochemical information

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A Unified Object Database for Biochemical Pathways

  • Jung, T.S.;Oh, J.S.;Jang, H.K.;Ahn, M.S.;Roh, D.H.;Cho, W.S.
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • pp.383-387
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    • 2005
  • One of the most important issues in post-genome era is identifying functions of genes and understanding the interaction among them. Such interactions from complex biochemical pathways, which are very useful to understand the organism system. We present an integrated biochemical pathway database system with a set of software tools for reconstruction, visualization, and simulation of the pathways from the database. The novel features of the presented system include: (a) automatic integration of the heterogeneous biochemical pathway databases, (b) gene ontology for high quality of database in the integration and query (c) various biochemical simulations on the pathway database, (d) dynamic pathway reconstruction for the gene list or sequence data, (e) graphical tools which enable users to view the reconstructed pathways in a dynamic form, (f) importing/exporting SBML documents, a data exchange standard for systems biology.

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A Novel Strategy for Thermostability Improvement of Trypsin Based on N-Glycosylation within the Ω-Loop Region

  • Guo, Chao;Liu, Ye;Yu, Haoran;Du, Kun;Gan, Yiru;Huang, He
    • Journal of Microbiology and Biotechnology
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    • v.26 no.7
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    • pp.1163-1172
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    • 2016
  • The Ω-loop is a nonregular and flexible structure that plays an important role in molecular recognition, protein folding, and thermostability. In the present study, molecular dynamics simulation was carried out to assess the molecular stability and flexibility profile of the porcine trypsin structures. Two Ω-Loops (fragment 57-67 and fragment 78-91) were confirmed to represent the flexible region. Subsequently, glycosylation site-directed mutations (A73S, N84S, and R104S) were introduced within the Ω-loop region and its wing chain based on its potential N-glycosylation sites (Asn-Xaa-Ser/Thr consensus sequences) and structure information to improve the thermostability of trypsin. The result demonstrated that the half-life of the N84S mutant at 50℃ increased by 177.89 min when compared with that of the wild-type enzyme. Furthermore, the significant increase in the thermal stability of the N84S mutant has also been proven by an increase in the Tm values determined by circular dichroism. Additionally, the optimum temperatures of the wild-type enzyme and the N84S mutant were 75℃ and 80℃, respectively. In conclusion, we obtained the thermostability-improved enzyme N84S mutant, and the strategy used to design this mutant based on its structural information and N-linked glycosylation modification could be applied to engineer other enzymes to meet the needs of the biotechnological industry.

A Longitudinal Study on Seasonal Variation of Riboflavin Status of Rural Women : Dietary Intake, Erythrocyte Glutathione Reductase Activity Coefficient, and Urinary Riboflavin Excretion (식이섭취와 적혈구 Glutathione Reductase 활성도 및 소변배설에 근거한 일부 농촌여성들의 리보플라빈 영양상태에 관한 종단연구)

  • 임화재
    • Journal of Nutrition and Health
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    • v.29 no.5
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    • pp.507-516
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    • 1996
  • This study was undertaken to estimate seasonal variation of riboflavin status and investigate the relationship between riboflavin intake and its biochemical status in rural women. Dietary intake was measured by determining both 24hr recall method and conventient method. The riboflavin intake was also estimated by food frequency method. Ribofiavin biochemeical status was assessed by erythrocyte glutathione reductase activity coefficient(EGR AC) and urinary riboflavin excretion. All information was repeatly collected in three seasons ; farming season (June), harvest season(October), nonfarming season(February). Mean daily riboflavin intake was below RDA for Koreans in all seasons. Cereal & pulse, vegetable were the primary sources of riboflavin intake and provided above 60% of the total dietary riboflavin intake in all seasons. Riboflavin biochemical status was significantly different among seasons(EGR AC P<0.005, 24hr urinary riboflavin excretion P<0.05), and riboflavin biochemical deficiency was highest in February. The mean values of EGR AC revealed riboflavin deficiency in all seasons. However the mean values of urinary riboflavin excretion were within the normal range except 24hr urinary riboflavin excretion in February. On the basis of EGR AC criteria, 44.7% of subjects were at risk of deficiency in June, 44.7% in October, 81.6% in February. Result of individual riboflavin status assessed by EGR AC or 24hr urinary riboflavin excretion criteria was quite alike. Urinary riboflavin excretion per gram of creatinine underestimated riboflavin deficiency. Significant correlations were observed among biochemical indexes. It also appeared that riboflavin intake measured by food frequency method showed significant correlation with biochemical indexes, especially high correlation with EGR AC (P<0.001). The results indicated that the prevalence of riboflavin deficiency seems to be high among rural women in all seasons and the inadequacy of dietary riboflavin intake was reflected in the abnormalcy of riboflavin biochemical status assessed by EGR, AC. Therefore EGR AC seems to be more sensitive measure of impaired riboflavin status compared to urinary excretion.

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A Case of Non-alcoholic Steatohepatitis Treated with Herbal Medicine

  • Son, Chang-Gue
    • The Journal of Korean Medicine
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    • v.32 no.3
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    • pp.50-54
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    • 2011
  • Objective: To understand the characteristic of non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH), and study the traditional Korean medicine (TKM)-based strategies or therapeutics for them. Methods: A male patient with NASH was treated with only Oriental therapies, and then the clinical outcome was evaluated by serum biochemical parameters and radiographs. Result: The clinical and biochemical values of the patient fluctuated over three years according to the treatments and cessation of herbal medicines. Conclusion: NASH or NAFLD are now prevalent and these disorders could be targets of TKM, and this case report would provide useful information.

A Case of Cold Medicine-Induced Hepatitis Treated with Herbal Medicine

  • Son, Chang-Gue
    • The Journal of Korean Medicine
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    • v.32 no.6
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    • pp.112-116
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    • 2011
  • Objectives: To inform the clinical features of drug-induced liver injury (DILI), and study traditional Korean medicine (TKM)-based strategies or therapeutics. Methods: A female patient with hepatitis after long term use of medication for cold symptoms was treated with Oriental therapies, after which the clinical outcome was evaluated by serum biochemical parameters and ultrasonography. Results: Clinical and biochemical levels were fluctuating during administration of cold medicine, but the patient completely recovered her health with herbal medicines. Conclusion: This case report would provide information about a typical DILI by western medicine followed by treatment with traditional Korean medicine.

Effect of Solvents on the Photochromic Properties of Spiropyran in Hard Coating Films Prepared by Sol-Gel Method (Spiropyran의 분산용매가 Sol-Gel 하드 코팅 막의 광 변색 특성에 미치는 영향)

  • Kim, Dae Hyun;Shin, Yong Tak;Lee, Ju Yeon;Hong, Wongil;Lee, Bum Suk;Song, Ki Chang
    • Korean Chemical Engineering Research
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    • v.47 no.4
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    • pp.430-435
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    • 2009
  • Spiropyran-doped organic-inorganic hybrid coatings were prepared starting from glycidoxypropyl triethoxysilane and vinyltriethoxysilane by a sol-gel method. They were applied as a thin layer to polycarbonate sheets and their photochromic properties were investigated. The effect of polarity of solvents dissolving the spiropyran was investigated on the photochromic properties. The decoloration rate of the spiropyran decreased with increasing the polarity of solvents dissolving the spiropyran because the open form of the spiropyran was easily stabilized in the polar gel matrix.

3D Bioprinting Technology in Biochemical Engineering (바이오화학공학에서 3D 바이오프린팅 기술)

  • Eom, Tae Yoon
    • Korean Chemical Engineering Research
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    • v.54 no.3
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    • pp.285-292
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    • 2016
  • Three-dimensional (3D) printing is driving major innovation in various areas including engineering, manufacturing, art, education and biosciences such as biochemical engineering, tissue engineering and regenerative medicine. Recent advances have enabled 3D printing of biocompatible materials, cells and supporting components into complex 3D functional tissues. Compared with non-biological printing, 3D bioprinting involves additional complexities which require the integration of technologies from the fields of biochemical engineering, biomaterial sciences, cell biology, physics, pharmaceutics and medical science.