• 수산해양기술연구
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• 제50권4호
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• pp.447-454
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• 2014

The marine microalgae Chaetoceros calcitrans was cultured under a fluorescent lamp (CON) and light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; green, LG; white, LW); changes in growth, fucoxanthin, chlorophyll-a, amino acid and fatty acid profiles were investigated. LR-exposed cultures exhibited the highest specific growth rate (SGR) (0.34), whereas LG-exposed cultures showed the lowest SGR (0.26). After cultivation for 10 days, the maximum dry cell weight (g/L) of LR-exposed cultures was significantly higher than that of those exposed to other light conditions (LR${\geq_-}$CON>LB${\geq_-}$LW${\geq_-}$LG). Eicosapentaenoic acid (EPA) levels were significantly higher in CON-exposed cultures compared to those exposed to LW (P<0.05), with no marked difference compared to those exposed to LB, LR and LG (P>0.05). The fucoxanthin content was highest in LB-exposed cultures ($6.3{\mu}g/mL$), whereas LW showed the lowest ($3.6{\mu}g/mL$; P<0.05). Chlorophyll-a content was highest in cultures exposed to LB compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

• 한국잡초학회지
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• 제31권2호
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• pp.152-159
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• 2011

산성비에 대한 봉선화의 피해양상과 지질조성 변화 및 방어기작 등을 조사하기 위하여 2주 동안 여러 농도(pH 2.0, 3.0, 4.0, 5.6)의 인공산성비 실험을 수행하였다. 인공산성비의 pH가 낮을수록 생육피해가 심하게 나타났다. 인공산성비의 pH가 낮을수록 엽록소 함량은 감소하였으며 $H_2O_2$ 함량은 증가하였다. 인지질과 당지질 모두 인공산성비에 의해 포화지방산이 증가하고 불포화지방산이 감소하는 것으로 나타났다. 봉선화에는 주로 3종류의 polyaimne이 존재하며 이들 모두 인공산성비의 pH가 낮을수록 증가하는 것으로 나타났다. Catalase의 활성도 인공산성비의 pH가 낮을수록 증가하였다. 이상의 결과를 종합해 볼 때, 인공산성비에 의해 활성산소 생성되고, 지방산의 변화를 일어나며, polyamine 증가와 catalase 활성 증가는 산성비의 피해를 최소화하기 위한 방어기작이 작용한 것으로 사료된다.

• 미생물학회지
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• 제21권3호
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• pp.115-126
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• 1983

The results that the effect of 6 detergents on the structural changes and biochemical composition of bacterial membrane of Escherichia coli and Bacillus cereus are as follows ; 1. Population growth of the bacteria was increased in case of the treatment with palmitoyl carnitine and sodium deoxy cholate but was increased in case of the treatment with palmitoyl carnitine and sodium deoxy cholate but was decreased by sodium dodecyl sulfate and palmitoyl choline, in E.coli and was decreased by palmitoyl carnitine and palmitoyl choline at the low concentration, in B. cereus. 2. The electron micrograph showed that cell wall lysis or cell collapse were observed in the treatment of sodium dodecyl sulfate and palmitoyl choline, and also cell wall was condensed by triton X-100 and sodium deoxy cholate, in E.coli. And in B. cereus, endospore formation of the bacteria was stimulated by palmitoyl choline, and cell lysis or structural changes of the membrane were observed in the treatment of sodium dodecyl sulfate, sodium cholate, and triton X-100, respectively. 3. As to the effect of detergent on the biochemical composition of biomembrane, the content of carnitine, in E.coli, and B.cereus, the content of structural protein and phospholipid were decreased by treatment of sodium dodecyl sulfate and structural protein was denatured by palmitoyl choline. 4. The profile of membrane protein revealed that the bacterial membrane were composed of various proteins. By dint of this result, some of membrane proteins were solubilized or changed to small molecules by the treatment of sodium dodecyl sulfate and palmitoyl choline, in E.coli and membrane protein of the biomembrane by treatment of sodium dodecyl sulfate, sodium deoxy cholate, palmitoyl choline, and palmitoyl carnitine were confirmed to be different profile as compared with those of the control, in B. cereus. Therefore, it is suggested that sodium dfodecyl sulfate and palmitoyl choline soulbilized biomembranes or inhibited membrane transport and that palmitoyl carnitine and sodium deoxy cholate were used as an energy source or stimulating the membrane transport, in E.coli. And, it is suggested that all of detergents were inhibited biomembrane synthesis, expet saponin, in B.cereus.

• 한국수산과학회지
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• 제46권3호
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• pp.259-265
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• 2013

The marine microalgae Nannochloropsis sp. was cultured under a metal halide lamp (MH) or light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; yellow, LY; green, LG; white, LW); changes in growth, total carotenoid, chlorophyll a, amino acid and fatty acid profiles were investigated. LB-exposed cultures exhibited the highest specific growth rate (SGR) (0.32), whereas LY-exposed cultures showed the lowest SGR (0.18). After cultivation for 9 days, the maximum dry cell weight (g/L) of LB-exposed cultures was significantly higher than that of those exposed to other light conditions (LB>MH>LW$${\geq_-}LG{\geq_-}$$LR>LY). The essential amino acid (EAA, %) contents of cultures exposed to LG, LB, LR and MH were higher than those exposed to LY and LW (P<0.05). Eicosapentaenoic acid and n-3 highly unsaturated fatty acid levels were significantly higher in MH-exposed cultures compared to those exposed to LY (P<0.05), with no marked difference compared to those exposed to LB, LG, LR and LW (P>0.05). The total carotenoid content was highest in LR-exposed cultures (18.0 mg/L), whereas MH showed the lowest (11.8 mg/L; P<0.05). Chlorophyll a content was highest in cultures exposed to LR compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

• 대한한의학회지
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• 제26권1호
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• pp.103-114
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• 2005

Objective; Childhood obesity has become one of the most common health problems facing children in the world. Although a number of regimens have been developed and distributed for the treatment of adult obesity, few studies have focused on therapeutic programs for obese children. The objective of this study was to develop and measure the effects of Taeumin childhood obesity by a sasang constitution­based education program. Methods ; A total of 14$(\%RBW\geq20)$ among 18 taeumin with childhood obesity from June to August 2003 participated in the constitution-based education program. It was designed to examine the effects of a constitution-based education program through anthropometric measurements, body composition indicates, biochemical indicates and serum level of taeumin childhood obesity. Results; The results of this study were as follows: 1. The anthropometric measurement of subscapular skinfold thickness was reduced after 4 weeks of the constitution-based education program. 2. The body composition indicates reduction in degree of obesity, percent body fat, and fat distribution. The other side increased in height, total body water, soft lean mass, and lean body mass. 3. For biochemical indicates, HDL-cholesterol was increased significantly after 4 weeks of the constitution-based education program. The other side decreased in AST and ALT. 4. There was a significant reduction in BMI and $\%RBW$ after the constitution-based education program. Conclusion : A constitution-based education program for weight control results in reduced degree of obesity among taeumin children.

• 한국수산과학회지
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• 제30권3호
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• pp.349-354
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• 1997

6개월, 12개월 및 20개월간 사육한 넙치를 즉살하여 암수를 구분한 후 등육을 취하여 각각의 사육기간 별로 근원섬유의 ATPase 활성, 열안정성 및 각 단백질의 subunit조성을 실험, 검토하였다. 6개월 사육한 수컷의 근원 섬유의 ATPase 활성은 암컷에 비하여 높았으며, 특히 $Mg^{2+}\;(+Ca^{2+})$-와 $Ca^{2+}-ATPase$ 활성에서 현저한 차이를 나타내었다. 12개월간 사육한 넙치에 있어서도 6개월간 사육한 것과 비슷한 경향을 보였으며, 20개월간 사육한 넙치 역시 유사한 경향을 나타내어 주었다. 또한 사육기간에 따라서 근원섬유의 ATPase 활성이 차이를 나타내었는데, 특히 $Mg^{2+}\;(+Ca^{2+})-ATPase$ 활성이 성장이 활발한 6개월째 사육한 넙치에서 가장 높았으며, 그 다음으로 12개월과 20개월간 사육한 순으로 나타나 성장 속도와 근원섬유의 ATPase 활성간에 높은 상관관계가 있음을 암시하였다. 근원섬유의 열안정성에 있어서도 수컷이 암컷에 비하여 현저히 떨어지는 경향을 보였다.

• Ocean and Polar Research
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• 제33권1호
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• pp.35-43
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• 2011

Blubber plays different biological roles in cetaceans, including structural support, physiological demands (thermoregulation, streamlining, buoyancy, etc.), and energy storage. As such, biochemical composition, especially lipid, in the blubber may be vertically stratified in order to effectively assist with these functions. We analysed lipid content, class and fatty acid composition in the blubber of by-catch minke whale (Balaenoptera acutorostrata; n = 11), long-beaked common dolphin (Delphinus capensis; n = 3) and Pacific white-sided dolphin (Lagenorhynchus obiquidens; n = 2) from Korean waters. Lipid content for all three species ranged from 55 to 96% of wet weight, with a dominance of triacylglycerols. Vertical stratification of blubber lipid content was different between species. For minke whale and longbeaked common dolphin, it increased from inner (near the muscle) to outer (near the skin) layer, whereas it decreased for Pacific white-sided dolphin. Thirty-one fatty acids were found in all blubber samples and vertical stratification of fatty acid composition was similar among all three species. Among grouped fatty acids, monounsaturates were the most abundant, gradually increasing from inner to outer blubber layer, whereas polyunsaturates and saturates decreased. Such vertical stratification of lipid content and composition suggests that the modification and accumulation of lipid occur in the blubber itself in order to help cetaceans with their specific physiological needs.

• Journal of Nutrition and Health
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• 제16권2호
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• pp.115-124
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• 1983

The biochemical nature of the protein constituents of six year old fresh Panax ginseng root was studied. Total protein constituents were extracted with phosphate buffer of pH 7.4, ionic strength of 0.1 and fractionated by ultrafiltration using four different membranes which cut down the materials of molecular weight of 500, 1,000, 5,000 and 10,000, respectively. Each fraction was subjected to ion exchange chromatography using DEAE - cellulose to isolate component proteins. The protein fraction larger than molecular weight of 10,000 was refractionated by the method of ammonium sulfate precipitation. The electrophoresis of the refractionated protein constituents was performed. The amino acid composition of the protein constituents was determined by gas- liquid chromatography. From the results, it could be summarized that eleven different protein constituents smaller than molecular weight of 10,000 were isolated from the fresh Panax ginseng root. At least eleven different protein constituents larger than molecular weight of 10,000 were identified from the electrophoretic patterns. These protein costituents seem to be compounded of all or some of five different subunits.

• Nutritional Sciences
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• 제9권3호
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• pp.190-194
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• 2006

Silajit is a blackish brown pitch-like gummy substance. It is an important Unani and Ayurvedic medicine widely used in the treatment of many diseases and as nutritional supplement It is found in certain mountainous regions of Pakistan, Afghanistan, India, Nepal, Australia and Russia. Silajit samples of Pakistani origin were obtained from different sources and analyzed for their physical characteristics and biochemical & elemental composition. X-ray diffraction studies on Silajit showed that it is an amorphous substance and has no crystalline structure. Also, pH studies of Silajit (2 g/L of water) indicated that it was slightly acidic in nature and ranged from 3.45 to 7.23. Conductivity ranged from 157.7 to 330. Amino acid analysis revealed that Silajit contained lysine and alanine in higher amounts than all other amino acids and ranged from 1456 to 2240 and 68 to $1615{\eta}mole/g$, respectively. Mean concentration of arsenic, mercury, cadmium, lead, copper and zinc was 73.15, 104.92, 0.496, 3.89, 4.04 and 17.23 ppm, respectively. Silajit samples were also analyzed for calcium, potassium and sodium.

• 한국식품과학회지
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• 제4권1호
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• pp.13-17
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• 1972

This experiment was conducted to observe the changes of composition of atmosphere in storage room by controlled ventilation under normal and sub-atmospheric conditions, and the biochemical changes in apple texture under these conditions. The results were as follows; 1) By controlled ventilation, concentration of carbon dioxide in storage room was optionally regulated and constantly maintained. 2) Among compositions of internal atmosphere of apple, especially, concentration of ethylene was much decreased at apple stored under sub-atmospheric condition than that stored under normal atmospheric condition. 3) Acidity of apple stored under sub-atmospheric condition was much greater than that of apple stored under normal atmospheric condition. 4) Respiratory quotients of apple, which was stored under normal and sub-atmospheric conditions, were shown to be high. But decarboxylation of added pyruvate was found more active in slices prepared from apple stored under sub-atmospheric condition.