• Title/Summary/Keyword: Biochemical calculation

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Thermal Dissociation and Conformational Lock of Superoxide Dismutase

  • Hong, J.;Moosavi-Movahedi, A.A.;Ghourchian, H.;Amani, M.;Amanlou, M.;Chilaka, F.C.
    • BMB Reports
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    • v.38 no.5
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    • pp.533-538
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    • 2005
  • The kinetics of thermal dissociation of superoxide dismutase (SOD) was studied in 0.05 M Tris-HCl buffer at pH 7.4 containing $10^{-4}\;M$ EDTA. The number of conformational locks and contact areas and amino acid residues of dimers of SOD were obtained by kinetic analysis and biochemical calculation. The cleavage bonds between dimers of SOD during thermal dissociation and type of interactions between specific amino acid residues were also simulated. Two identical contact areas between two subunits were identified. Cleavage of these contact areas resulted in dissociation of the subunits, with destruction of the active centers, and thus, lost of activity. It is suggested that the contact areas interact with active centers by conformational changes involving secondary structural elements.

Construction of Greenhouse Gas Inventory of Private Industry of Chungcheongbuk-do and Analysis of Greenhouse Gas Mitigation Technology (충청북도 민간 산업체에 대한 온실가스 인벤토리 구축 및 감축기술 분석)

  • Lim, Soo Min;Ahn, Joo Young;Jung, Cho Shi;Park, Jung Hoon
    • Journal of Climate Change Research
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    • v.8 no.1
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    • pp.57-62
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    • 2017
  • Greenhouse gas (GHG) emissions of private industry of Chungcheongbuk-do were estimated. GHG emissions were classified by industry and GHG emissions ratio of each industry of Chungcheongbuk-do was found. Characteristics of GHG emissions of Chungcheongbuk-do and GHG mitigation technology were analyzed. To calculate GHG emissions, equations proposed through GHG emissions calculation guidelines published by Korean Energy Agency in 2009 were used. As a result, GHG emissions ratio of cement, semiconductor, paper and petrochemical industry was about 73%, 16%, 5%, and 2% respectively. GHG mitigation technologies of cement, semiconductor and waste were investigated. For cement, amine technology, for semiconductor, scrubber system and for waste, land fill gas utilization were analyzed.

Ethanol Production from Sago Starch Using Zymomonas mobilis Coentrapped with Amyloglucosidase (동시고정화된 Amyloglucosidase와 Zymomonas mobilis를 이용한 전분으로부터의 Ethanol 생산)

  • Kim, Chul-Ho;Lee, Gyun-Min;Han, Moon-Hi;Rhee, Sang-Ki
    • Microbiology and Biotechnology Letters
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    • v.15 no.6
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    • pp.430-435
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    • 1987
  • A chitin-immobilized enzyme amyloglucosidase(AMG) and a bacterium Zymomonas mobilis were coentrapped in alginate gel beads. Ethanol production was performed in a packed bed column reactor in a simultaneous saccharification and fermentation(SSF) mode using liquefied sago starch as a substrate. It was found that this process eliminated product inhibition and reverse reaction of glucose enhancing the rate of saccharification and ethanol production. At a low dilution rate of D = 0.11 hr$^{-1}$, the steady-state ethanol concentration was 46.0g/$m\ell$ (96.8 % of theoretical yield). The maximum ethanol productivity was 17.7g/$m\ell$, h at D = 0.83 hr$^{-1}$ when the calculation was based on the total working volume. The continuous production of ethanol was maintained stably over 40 days without problems in this reactor system.

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Design of an Absorption Heat Transformer with Methanol-Glycerine System as a Working Fluid (메탄올-글리세린 계를 작동유체로 하는 변형흡수식 열펌프 설계)

  • Chung, Chan-Kyo;Min, Byong-Hun
    • Clean Technology
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    • v.11 no.1
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    • pp.13-19
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    • 2005
  • An absorption heat transformer for energy recovery has been investigated using methanol-glycerine. The simulated calculation of theoretical thermal efficiency was performed based on the thermodynamic properties of the working fluid over various operating conditions. The thermal efficiency of higher than 0.4 was obtained by raising industrial waste heat, $70-80^{\circ}C$, by $40^{\circ}C$ in this system.

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Vertical composition and character analysis of saprophytic bacteria isolated from the mudflat of Nakdong river estuary (낙동강 하구 간석지에서 분리된 세균의 층별 종조성 및 특성에 관하여)

  • Hong, Soon-Woo;Kim, Sang-Jong;Rhie, Youn;Choi, Sung-Chan
    • Korean Journal of Microbiology
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    • v.23 no.2
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    • pp.157-165
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    • 1985
  • Bacterial identification was performed with morphological, physiological and biochemical tests to the isolates from the mudflat of 30cm depth sampled in Nakdong river estuary in March and June, 1985. Flavobacterium and Cnterobacteriaceae were regarded as dominants. Pseudomonas, Bacillus, Micrococcus, Vibrio, Aerococcus, Aerononas, Acinetobacter and Staphylococcus were founded in various depth. Vertical composition of bacterial genera in March was more diversiform than that of June. Character analysis was carried out with the calculation of similarity index (S). At a level of 85% similarity, the isolates were clustered into 5 groups and ungrouped 2 strains. Classifying groups of bacterial strains with determination schemes and groups from similarity index were in good agreement.

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The Calculation of NPS Load per Unit Area in Orchard to the Nakdong River Basin (낙동강유역 과수재배지의 단위면적당 비점오염부하량 산정에 관한 연구)

  • Lee, Jae-Woon;Kwon, Heon-Gak;Yi, Youn-Jeong;Cheon, Se-Uk
    • Journal of Environmental Impact Assessment
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    • v.22 no.6
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    • pp.557-568
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    • 2013
  • In this study, Calculated the nonpoint sources(NPS) load per unit area about various rainy events in vineyard of Nakdong River basin. NPS monitoring and calculation for NPS load per unit area were estimated from 'Investigation method of precipitation discharge(National Institute of Environmental Research, 2007)'. The evaluation of applicability for NPS load per unit by compared with prior research data and Total Maximum Daily Load(TMDL) data. Five target areas were each $2000m^2$, $1800m^2$, $1943m^2$, $2484m^2$, $864m^2$ and located in Gyeongsangbukdo Gyeongju, Gyeongsangbukdo Sangju, Gyeongsangnamdo Hapcheon in Korea. Since fruits were the only crop on the target area, the characteristics of stormwater discharge at survey sites could be evaluated independently. A total of 115 rainfall events in the Orchard area during five years(2008-2012) was surveyed, and 38 of them became stormwater discharge. In the Nakdong River watershed, average of event mean concentrations(EMCs) in Orchard area for biochemical oxyzen demand(BOD), Chemical oxyzen demand(COD), total nitrogen(T-N), total phosphorus(T-P) were 2.0mg/L, 10.1mg/L, 3.195mg/L, 0.578mg/L, respectively. NPS load per unit area in Orchard area showed BOD : $2.0kg/km^2{\cdot}day$, COD : $10.2kg/km^2{\cdot}day$, T-N : $3.220kg/km^2{\cdot}day$, T-P : $0.606kg/km^2{\cdot}day$.

Manipulation System for Nutrition Counseling Based on Internet (인터넷 영양상담관리 시스템)

  • Hong, Sun-Myeong;Kim, Gon
    • Journal of the Korean Dietetic Association
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    • v.10 no.3
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    • pp.284-292
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    • 2004
  • The purpose of this study was to develop a manipulation system for nutrition counseling based on internet. This system offers convenient user interface and the synthetic counseling results with various utilities. This system consists of the general information of clients, the anthropometry data and the calculation of obesity and body index, the state of eating habits, calorie expenditure, clinical symptoms, the convenient method for analysis of nutrients, biochemical data and nutrition prescription. Having interoperability, these functions preserve the information of clients and manage the historical data. This system can insert, store, print out and generate the synthetic information of clients to provide a suitable and efficient nutrition counseling information. With accumulated client data, It does the nutrition education and counseling simultaneously. As it is developed based on internet, it provides friendly user interface. Also, Managing clients' information connected to database, it can provide a systematic and formal information. It is possible for the system to retrieve information and counsel in real time. It is expected that the nutrition counseling management system can improve the national health with animated nutrition counseling.

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Calculation of Total Amount of Pollutant Load by Sub-Watersheds in the Anyangcheon Watershed (안양천 유역의 소유역별 오염총량 산정)

  • Lee, Kil-Seong;Kim, Kyung-Tae;Kim, Sang-Ug;Chung, Eun-Seong
    • Proceedings of the Korea Water Resources Association Conference
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    • 2008.05a
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    • pp.1981-1984
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    • 2008
  • 기존 하천에 실시되고 있던 농도 규제의 근본적인 한계를 극복하고자 우리나라 4대강에 오염 총량관리제도가 실시되었다. 그러나 1999년부터 약 8년 동안 실시된 오염총량관리제도에 기술적 제도적 문제들이 발견되고 있다. 본 연구에서는 오염총량관리제도의 많은 문제점들 중에 기준유량에 대한 문제점과 오염부하량의 할당에 대한 문제점에 중점을 두었다. 총량관리단위유역은 대표적인 도시하천 중 하나이며 한강의 지류인 안양천 유역이며, 목표수질은 1991년 환경부 하천환경수 질기준인 5등급으로 결정하였으며, 관리물질은 1차관리물질인 BOD(Biochemical Oxygen Demand)이다. 기준유량은 평균저수량과 평균갈수량을 사용하여 비교하였고, 발생부하량을 계산하였으며, 발생부하량에 의한 배출부하량을 계산하고, HSPF(Hydrologic Simulation Program - FORTRAN) 모형을 사용하여 배출부하량으로 인한 유달부하량을 산정하여 허용부하량을 계산하였다. 마지막으로 목표수질을 만족시키기 위해 삭감부하량을 계산하였고, 삭감부하량을 안양천 유역의 각 소유역별로 할당하였다.

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Estimation of CBOD Decay Rate for the Execution of Water Quality Model in the Nakdong-River Basin (낙동강에서 수질모델 실행을 위한 탈산소계수의 평가)

  • Yu, Jae-Jeong;Yoon, Young-Sam;Lee, Hae-Jin;Kim, Moon-Soo;Yang, Sang-yong;Lee, Young Joon
    • Journal of Korean Society on Water Environment
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    • v.21 no.5
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    • pp.511-515
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    • 2005
  • CBOD(carbonaceous BOD) decay rate was investigated for the execution of water quality model in Nakdong-Rive basin. Estimation of laboratory-derived CBOD decay rate, $k_l$ and CBOD decay rate in natural waters, $k_d$ were carried out. Hydraulic factors were applied for the calculation of $k_d$. Values of biochemical oxygen demand were investigated in Weagwan, Koreong, Jeokpo, Namgi and Mulgeom sites for the four times. The ranges of $k_l$ value were $0.04{\pm}0.01{\sim}0.14{\pm}0.03$. The values of $k_l$ in upstream sites were much larger than those in the downstream sites. The values of $k_d$ were 0.025, 0.036, 0.005 and 0.001 at Weagwan, Jeokpo, Namgi and Mulgeom, respectively, indicating that values of $k_d$ were generally larger than those of $k_l$.

Stage by stage design for primary, conventional activated sludge, SBR and MBBR units for residential wastewater treatment and reusing

  • Aziz, Shuokr Qarani;Omar, Imad Ali;Bashir, Mohammed J.K.;Mojiri, Amin
    • Advances in environmental research
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    • v.9 no.4
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    • pp.233-249
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    • 2020
  • To date, there is no central wastewater (WW) treatment plant in Erbil city, Kurdistan region, Iraq. Therefore, raw WW disposes to the environment and sometimes it used directly for irrigation in some areas of Erbil city. Disposal of the untreated WW to the natural environment and using for irrigation it causes problems for the people and the environment. The aims of the current work were to study the characteristics, design of primary and different secondary treatment units and reusing of produced WW. Raw WW samples from Ashty city-Erbil city were collected and analyzed for twenty three quality parameters such as Total Suspended Solids (TSS), total dissolved solids, total volatile and non-volatile solids, total acidity, total alkalinity, total hardness, five-day Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD), biodegradability ratio (BOD5/COD), turbidity, etc. Results revealed that some parameters such as BOD5 and TSS were exceeded the standards for disposal of WW. Design and calculations for primary and secondary treatment (biological treatment) processes were presented. Primary treatment units such as screening, grit chamber, and flow equalization tank were designed and detailed calculation were illustrated. While, Conventional Activated Sludge (CAS), Sequencing Batch Reactor (SBR) and Moving Bed Biofilm Reactors (MBBR) were applied for the biological treatment of WW. Results revealed that MBBR was the best and economic technique for the biological treatment of WW. Treated WW is suitable for reusing and there is no restriction on use for irrigation of green areas inside Ashty city campus.