• Proceedings of the Korea Air Pollution Research Association Conference
• /
• 2008.10a
• /
• pp.446-448
• /
• 2008

• Asian Journal of Atmospheric Environment
• /
• v.5 no.3
• /
• pp.172-180
• /
• 2011

The transfer of microorganisms is important process for ecosystems. Microorganisms in dryland can transport itself to wetland through atmospheric diffusion, but only few papers reported about the atmospheric bioaerosol present over dryland. We carried out the direct sampling using a tethered balloon over Dunhuang City, China's northwestern dryland. Bioaerosols were collected using a tethered balloon with a bioaerosol collector at 820 m above the ground (1,960 m above the sea level) around noon on August 17, 2007. The bioaerosols were cultured after the collection at Dunhuang Meteorological observatory. Two strains of molds were isolated using the Nutrient agar medium. About 400-bp 18S rRNA partial sequences were amplified by PCR and determined afterwards. The results of a homology search by 18S rRNA sequences of isolates in DNA databases (GenBank, DDBJ, and EMBL) and an observation of the form revealed that two bioaerosols in the convective mixed layer over Dunhuang City were Cladosporium sp. and Aspergillus sp.

• Journal of Environmental Health Sciences
• /
• v.32 no.5 s.92
• /
• pp.478-484
• /
• 2006

There was no significant difference in the CADR (Clean Air Delivery Rate) between physical aerosols, NaCl and smoke, and biological aerosols, airborne MS2 virus and P. fluorescens, which implicate that the hybrid-type of air purifier, applying the unipolar ion emission and the radiant catalytic ionization, imposed identical reduction effect on both physical aerosol and bioaerosol. Ventilation decreases the efficiency of air cleaning by unipolar ionization because high ventilation diminishes the particle concentration reduction effect. The particle removal efficiency decreases with increase in the chamber volume because of the augmented ion diffusion and higher ion wall loss rate. Particle size affects the efficiency of air ionization. The efficiency is high for particles with very small diameter because reduction of charge increases with particle size. If there is no increasing supply of ions, the efficiency of air cleaning by unipolar ionization increases with respect to initial concentration of particles because of the large space charge effect at high particle concentration and amplified electric field.

• Environmental Engineering Research
• /
• v.17 no.4
• /
• pp.211-216
• /
• 2012

Quantification of the airborne microorganisms (bacteria and fungi) at a swine wastewater treatment plant was performed. Microbial samples were collected at three different phases of the treatment process over a 1-yr period. Cultivation methods based on the viable counts of mesophilic heterotrophic bacteria and fungi were performed. The concentrations of airborne bacteria ranged up to about $5{\times}10^3$ colony-forming unit (CFU)/$m^3$, and those of airborne fungi ranged up to about $9{\times}10^2CFU/m^3$. The primary treatment (e.g., screen, grit removal, and primary sedimentation) was found to be the major source of airborne microorganisms at the site studied, and higher levels of airborne bacteria and fungi were observed in summer. High levels of the respirable bioaerosol (0.65 to $4.7{\mu}m$ in size) were detected in the aeration phase. Among the environmental factors studied, temperature was strongly associated with fungal aerosol generation (with a Spearman correlation coefficient of 0.90 and p-value <0.01). Occupational biorisks are discussed based on the observed field data.

• Particle and aerosol research
• /
• v.19 no.3
• /
• pp.89-100
• /
• 2023

In our previous study, a wire-plate type electrostatic precipitator (ESP) was developed to collect bioaerosols of 100 nm size. In the study, various flow rates (40 ~ 100 L/min) and applied voltages (3 ~ 10 kV) were tested for experiment. In this study, numerical analysis was performed for the ESP of the previous study with the same flow rates and applied voltages, but with varying the size of bioaerosols to 0.04 ~ 2.5 ㎛. Overall, the numerical analysis results well predicted the experimental data. Bioaerosols of 0.1 ~ 0.5 ㎛ showed the minimum collection efficiency for all conditions because of low charge number. The effect of the ionic wind generated by the corona discharge was calculated. However, the ionic wind did not affect much the collection efficiency. The aerosol collection in the ESP of this study was due to the electrostatic force generated by particle charge in the electric field. This numerical study on the ESP can be used for the design and optimization of higher flow rate (> 100 L/min) ESP.

• Journal of environmental and Sanitary engineering
• /
• v.20 no.4 s.58
• /
• pp.21-30
• /
• 2005

This research was performed to measure the concentration distribution of bioaerosol in apartment houses in the region of Chungnam (Chunan, Asan) for 1 month, December, 2004. The results are as follows. 1. By using SDA method, the average concentration of total microbe and fungus in the air inside and outside apartment house below 4 years are $69,42cfu/m^3\;and\;15.66cfu/m^3$, while apartment house over 4 years, $214.58cfu/m^3\;and\;216.43cfu/m^3$, respectively With gravitational sedimentation method, the average concentration of total microbe and fungus of apartmenthouse below 4 years are $100.63cfu/m^3\;and\;22.83cfu/m^3$, while apartment house over 4 years, $216.43cfu/m^3\;and\;70.000cfu/m^3$, respectively. 2. The I/O ratio of floating germ of apartment house below 4 years and over 4 years are 2.87 and 5.12 for total microbe, and 3.32 and 8.28 for fungus, respectively. The I/O ratio of falling germ of apartment house below 4 years and over 4 years are 1.55 and 2.81 for total microbe, and 2.85 and 4.08 for fungus, respectively. The apartment house below 4 years shows a low I/O ratio in all cases. 3. The difference in concentration of microbe between inside master bedroom and living room of apartment house below 4 years is $13.183cfu/m^3$, total microbe, and $4.787cfu/m^3$, fungus, while, apartment house over 4 years, $43.531cfu/m^3$, total microbe, and $21.932cfu/m^3$, fungus. The measured differences are statistically significant. 4. Air sampler was used to verify the difference in concentration of microbe with the age of apartment house. The difference in concentration of total microbe and fungus for outside apartment house below 4 years and over 4 years are $49.82cfu/m^3$ and $3.78cfu/m^3$, respectively. The difference of inside living room shows $160.23cfu/m^3$ for total microbe and $28.01cfu/m^3$ for fungus, and the difference of inside master bedroom shows $225.43cfu/m^3$ for total microbe and $56.73cfu/m^3$ for fungus. The differences are statistically significant. The difference in concentration of outside apartment house below 4 years and years 4 years are $34.66cfu/m^3$, total microbe, and $15.66cfu/m^3$, fungus, while inside apartment house below 4 years and over 4 years, $196.93cfu/m^3,\;and\;78.67cfu/m^3$, respectively. The measured differences are statistically significant.

• Proceedings of the Korea Air Pollution Research Association Conference
• /
• 2001.11a
• /
• pp.235-236
• /
• 2001

전 세계적으로 진행되고 있는 경제성장에 따른 급속한 산업발달은 인류에게 문명의 이기를 제공해 주고 있으나, 이에 수반하여 다양하고 새로운 오염물질(Bioaerosol)이 발생하고 있다. 이러한 새로운 오염 물질의 문제점은 그 피해 정도가 확실히 규명된 것이 아니라는 데 있으며, 또한 여러 경로를 통하여 오염될 수 있다는 데 있다. (중략)

• Journal of Environmental Health Sciences
• /
• v.40 no.2
• /
• pp.98-104
• /
• 2014

Objectives: The purpose of this study was to determine airborne bioaerosols such as bacteria and fungi in the kitchens of restaurants, and to assess the effects of thermal factors on the levels of the bioaerosols. Methods: Air samples were taken from kitchens of nine restaurants. An Anderson type air sampler was used for sampling and measurements. Petri dishes filled with a microbiological culture medium (trypticase soy agar for bacteria and Sabouraud dextrose agar for fungi) were used as the sampling surface. Results: The levels of bacterial aerosol measured were $10-10^3CFU/m^3$ and fungal aerosol $10-10^2CFU/m^3$, respectively The mean values of air temperature and relative humidity in the kitchens were $24.6^{\circ}C$ and 46.4%, respectively. Overall, the levels of bacterial aerosol varied by the restaurant type, and fungal aerosol by the business period (p < 0.05). The main effect of air temperature and interaction effect of air temperature and relative humidity onto the bacterial level were significant (p < 0.05), whereas the effects were not significant onto the fungal level. Conclusions: The results indicate a wide variation in the levels of bioaerosols among different kitchens. The observed differences in bioaerosol levels in the kitchens reflect different periods of use. The interactions of air temperature and relative humidity onto the bacterial level suggest that constant attention should be paid to avoid peaks of contamination during the summer season.

• Korean Journal of Microbiology
• /
• v.48 no.1
• /
• pp.8-15
• /
• 2012

Bioaerosols generated from composting facilities and landfills may create health risks for workers and nearby residents. To determine the levels of culturable airborne bacteria and fungi in bioaerosols, samples were seasonally collected at a composting facility and a landfill in Ulsan, Korea with an impaction-type sampler. Concentrations of heterotrophic bacteria averaged (in $MPN/m^3$) $6.5{\times}10^3$ (range $1.5{\times}10^2-1.5{\times}10^4$) in the composting facility and $3.9{\times}10^3$ (range $6.0{\times}10^1-9.3{\times}10^3$) at the entrance of the facility. These concentrations were 460 and 280 times higher than those of reference sites. Coliform bacteria were detected both inside and entrance of the facility. On the landfill, heterotrophic bacterial concentrations averaged (in $MPN/m^3$) $4.9{\times}10^2$ (range $1.7{\times}10^2-1.0{\times}10^3$), while they averaged $3.7{\times}10^2$ (range $4.8{\times}10^1-1.3{\times}10^3$) at the parking lot of the landfill. These concentrations were 35 and 26 times higher than those of reference sites. When we isolated and tentatively identified heterotrophic bacteria, Pseudomonas luteola was the most dominant species in bioaerosols from the composting facility, whereas the most abundant one in reference samples was Micrococcus sp. Average concentrations of airborne fungi were measured between $4.8{\times}10^2$ and $7.9{\times}10^2\;MPN/m^3$ depending on sites, which were 2.1-3.4 times higher compared to those of reference sites. While Cladosporium, Alternaria, and Penicillium were commonly identified fungal genera, genus Aspergillus was identified only in bioaerosols from the composting facility.

• Journal of Microbiology and Biotechnology
• /
• v.18 no.4
• /
• pp.795-798
• /
• 2008

As the first step to develop efficient means to control fungal spore bioaerosols, we designed, manufactured, and evaluated a fungal spore aerosol generator. We studied the physical and biological properties of the fungal spore bioaerosols on two common fungal species. The results demonstrated that the fungal spore bioaerosol generator effectively produces fungal spore bioaerosols.