• Title/Summary/Keyword: BioResource

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Disease and insect damage, growth and yield of sorghum, foxtail millet between rotational upland and continuing upland field

  • Yu, Je Bin;Yoon, Seong Tak;Yang, jing;Han, Tae Kyu;Jeong, In Ho;Kim, Young-Jung;Ye, Min Hee;Lee, Gil Jun;Cho, Soo Been;Lee, Young Kyung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.349-349
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    • 2017
  • This study was performed in order to investigate disease, insect damage, growth and yield characteristics of green maize by organic paddy-upland rotation system. This experiment also was to select optimum variety for organic paddy-upland rotation cultivation. This experiment was conducted at Anseong-si Gyeonggi province of Korea in 2016. The varieties used in this study are green maize of total 8 varieties. Green maize was planted at rotational upland field and continuing upland field and tested for comparison. In case of average occurrence of 4 major diseases for green maize, rotational upland field was higher than that of continuing upland field. Heukjinjuchal and Daehakchal were the lowest occurrence by less than 2% among 8 varieties. Average damage of 8 varieties by Ostrinia furnacalis larva, which is the main pest in green maize was higher in rotational upland field than that of continuing upland field. Chalok 4 and Heugjeom 2 were judged to be resistant varieties to 4 major diseases among 8 varieties. The average yield of green maize per 10a in rotational upland field decreased to 85% level of continuing upland field and Chalok 4 showed the highest yield by 789.0 kg/10a among 8 varieties. The most suitable varieties in organic paddy-upland rotation system were judged to be Chalok 4, Heukjinjuchal and Heukjeom 2.

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Antagonistic and Plant Growth-Promoting Effects of Bacillus velezensis BS1 Isolated from Rhizosphere Soil in a Pepper Field

  • Shin, Jong-Hwan;Park, Byung-Seoung;Kim, Hee-Yeong;Lee, Kwang-Ho;Kim, Kyoung Su
    • The Plant Pathology Journal
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    • v.37 no.3
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    • pp.307-314
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    • 2021
  • Pepper (Capsicum annuum L.) is an important agricultural crop worldwide. Recently, Colletotrichum scovillei, a member of the C. acutatum species complex, was reported to be the dominant pathogen causing pepper anthracnose disease in South Korea. In the present study, we isolated bacterial strains from rhizosphere soil in a pepper field in Gangwon Province, Korea, and assessed their antifungal ability against C. scovillei strain KC05. Among these strains, a strain named BS1 significantly inhibited mycelial growth, appressorium formation, and disease development of C. scovillei. By combined sequence analysis using 16S rRNA and partial gyrA sequences, strain BS1 was identified as Bacillus velezensis, a member of the B. subtilis species complex. BS1 produced hydrolytic enzymes (cellulase and protease) and iron-chelating siderophores. It also promoted chili pepper (cv. Nockwang) seedling growth compared with untreated plants. The study concluded that B. velezensis BS1 has good potential as a biocontrol agent of anthracnose disease in chili pepper caused by C. scovillei.

A New Record and Characterization of Asparagus Purple Spot Caused by Stemphylium vesicarium in Korea

  • Han, Joon-Hee;Shin, Jong-Hwan;Fu, Teng;Kim, Kyoung Su
    • Mycobiology
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    • v.47 no.1
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    • pp.120-125
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    • 2019
  • In 2017, small, elliptical, brownish purple spots on spears and ferns of asparagus were found in fields of Gangwon-do. The isolated fungal species was identified as an ascomycete Stemphylium vesicarium based on morphological characteristics and molecular phylogenic analyses including nucleotide sequences of the internal transcribed spacer (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and cytochrome b (cytb). A pathogenicity test revealed that S. vesicarium was the causal agent of purple spot disease on asparagus. The occurrence of purple spots caused by S. vesicarium on asparagus is the first report in Korea.

Dissemination of Advanced Mouse Resources and Technologies at RIKEN BioResource Center

  • Yoshiki, Atsushi
    • Interdisciplinary Bio Central
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    • v.2 no.4
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    • pp.15.1-15.5
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    • 2010
  • RIKEN BioResource Center (BRC) has collected, preserved, conducted quality control of, and distributed mouse resources since 2002 as the core facility of the National BioResource Project by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Our mouse resources include over 5,000 strains such as humanized disease models, fluorescent reporters, and knockout mice. We have developed novel mouse strains such as tissue-specific Cre-drivers and optogenetic strains that are in high demand by the research community. We have removed all our specified pathogens from the deposited mice and used our quality control tests to examine their genetic modifications and backgrounds. RIKEN BRC is a founding member of the Federation of International Mouse Resources and the Asian Mouse Mutagenesis and Resource Association, and provides mouse resources to the one-stop International Mouse Strain Resource database. RIKEN BRC also participates in the International Gene Trap Consortium, having registered 713 gene-trap clones and their sequences in a public library, and is an advisory member of the CREATE (Coordination of resources for conditional expression of mutated mouse alleles) consortium which represents major European and international mouse database holders for the integration and dissemination of Cre-driver strains. RIKEN BRC provides training courses in the use of advanced technologies for the quality control and cryopreservation of mouse strains to promote the effective use of mouse resources worldwide.

MoRBP9 Encoding a Ran-Binding Protein Microtubule-Organizing Center Is Required for Asexual Reproduction and Infection in the Rice Blast Pathogen Magnaporthe oryzae

  • Fu, Teng;Park, Gi-Chang;Han, Joon Hee;Shin, Jong-Hwan;Park, Hyun-Hoo;Kim, Kyoung Su
    • The Plant Pathology Journal
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    • v.35 no.6
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    • pp.564-574
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    • 2019
  • Like many fungal pathogens, the conidium and appressorium play key roles during polycyclic dissemination and infection of Magnaporthe oryzae. Ran-binding protein microtubule-organizing center (RanBPM) is a highly conserved nucleocytoplasmic protein. In animalia, RanBPM has been implicated in apoptosis, cell morphology, and transcription. However, the functional roles of RanBPM, encoded by MGG_00753 (named MoRBP9) in M. oryzae, have not been elucidated. Here, the deletion mutant ΔMorbp9 for MoRBP9 was generated via homologous recombination to investigate the functions of this gene. The ΔMorbp9 exhibited normal conidial germination and vegetative growth but dramatically reduced conidiation compared with the wild type, suggesting that MoRBP9 is involved in conidial production. ΔMorbp9 conidia failed to produce appressoria on hydrophobic surfaces, whereas ΔMorbp9 still developed aberrantly shaped appressorium-like structures at hyphal tips on the same surface, suggesting that MoRBP9 is involved in the morphology of appressorium-like structures from hyphal tips and is critical for development of appressorium from germ tubes. Taken together, our results indicated that MoRBP9 played a pleiotropic role in polycyclic dissemination and infection-related morphogenesis of M. oryzae.