• Title/Summary/Keyword: Bio-resources

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Use of Lycopene, an Antioxidant Carotinoid, in Laying Hens for Egg Yolk Pigmentation

  • Kang, D.-K.;Kim, S.-I.;Cho, C.-H.;Yim, Y.-H.;Kim, H.-S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.12
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    • pp.1799-1803
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    • 2003
  • The possibility of lycopene affecting egg yolk pigmentation was studied with lycopene diets containing 0, 4, 8, and $12{\mu}g/g$ meal, respectively. The addition of lycopene above $4{\mu}g/g$ meal significantly improved yolk color after four days of supplementation. The transfer of lycopene into egg yolk was confirmed by thin layer chromatography, and high-performance liquid chromatography-mass spectrometry (HPLC-MS). The deposition rate of lycopene into egg yolk was approximately 2%, which was quantitatively determined using a HPLC with a UV detector. The result indicates that lycopene is a good candidate for egg yolk pigmentation and for making functional eggs.

Cloning, Expression, and Functional Characterization of the Dunaliella salina 5-enolpyruvylshikimate-3-phosphate Synthase Gene in Escherichia coli

  • Yi, Yi;Qiao, Dairong;Bai, Linhan;Xu, Hui;Li, Ya;Wang, Xiaolin;Cao, Yi
    • Journal of Microbiology
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    • v.45 no.2
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    • pp.153-157
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    • 2007
  • 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19) is the sixth enzyme in the shikimate pathway which is essential for the synthesis of aromatic amino acids and many secondary metabolites. The enzyme is widely involved in glyphosate tolerant transgenic plants because it is the primary target of the nonselective herbicide glyphosate. In this study, the Dunaliella salina EPSP synthase gene was cloned by RT-PCR approach. It contains an open reading frame encoding a protein of 514 amino acids with a calculated molecular weight of 54.6 KDa. The derived amino acid sequence showed high homology with other EPSP synthases. The Dunaliella salina EPSP synthase gene was expressed in Escherichia coli and the recombinant EPSP synthase were identified by functional complementation assay.

First Report of Mortierella alpina (Mortierellaceae, Zygomycota) Isolated from Crop Field Soil in Korea

  • Yadav, Dil Raj;Kim, Sang Woo;Babu, Anam Giridhar;Adhikari, Mahesh;Kim, Changmu;Lee, Hyang Burm;Lee, Youn Su
    • Mycobiology
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    • v.42 no.4
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    • pp.401-404
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    • 2014
  • A new recorded species of Mortierella was recovered during the investigation of fungal communities in soil samples collected from different locations of Gangwon-do, Korea. The species was identified and described as Mortierella alpina on the basis of phylogenetic analysis of internal transcribed spacer sequences and morphological characteristics. This species has not been officially reported from Korea thus far.

Improvement of the Quality of Dried Wild Vegetables by Micro Oil Sprayed Thermal Air Technique

  • Yonghyun Kim;Yunmi Park;Chul-Woo Kim;Uk Lee;Hyun Ji Eo
    • Korean Journal of Plant Resources
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    • v.35 no.6
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    • pp.778-784
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    • 2022
  • Wild vegetables, such as Cirsium setidens, and Aster scaber, are commonly distributed as dried materials because the wild vegetables lose their freshness quickly after harvest and distribution. Dried wild vegetables require rehydration to use as a food ingredient, and the quality of rehydrated wild vegetables is affected by pre-drying and drying methods. Here, we introduce the newly developed pre-drying and drying method, termed "micro oil sprayed thermal air (MOTA) technique". The three wild vegetables processed by the MOTA technique showed improved rehydration rate and reduced time to achieve maximum rehydration rate. Color characteristics were also improved in C. setidens. These results indicate that the MOTA technique improves the overall quality of rehydrated wild vegetables. It is expected that our findings could enhance the marketability of dried wild vegetables by improving overall quality and reducing preparation time.