• 접착 및 계면
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• 제3권3호
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• pp.41-50
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• 2002

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2003년도 추계 학술발표회
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• pp.173-173
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• 2003

• 한국작물학회:학술대회논문집
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• 한국작물학회 2004년도 춘계 학술대회지
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• pp.60-61
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• 2004

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 춘계학술대회
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• pp.165-165
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• 2020

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2003년도 제2차 연례학술대회 발표논문집
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• pp.245-250
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• 2003

There are currently about 6000 bacterial species with validly published names, but scientists assume that these may be less than 1% of bacterial species present on the earth. Microbial resource is one of the most important bioresources in bioinderstry and provides us with high economic values. To find missing ones, the studies of metagenome, metabolome, and proteome about microbes have started recently in developed countries. We construct the information system that integrates information on microbial genome resources and manages the information to support efficient research of microbial genome application, and name this system 'Bio-Meta Information System (Bio-MIS)'. Bio-MIS consists of integrated microbial genome resources database, microbial genome resources input system, integrated microbial genome resources search engine, microbial resources on-line distribution system, portal service and management via internet. In the future, we will include public database connection and implement useful bioinformatics software for analyzing microbial genome resources. The web-site is accessible at http://biomis.probionic.com

• Environmental Engineering Research
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• 제25권1호
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• pp.18-28
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• 2020

In this paper the authors provide comparative evaluation of current research that used liquefaction and pyrolysis method for bio-oil production from various types of biomass. This paper review the resources of biomass, composition of biomass, properties of bio-oil from various biomass and also the utilizations of bio-oil in industry. The primary objective of this review article is to gather all recent data about production of bio-oil by using liquefaction and pyrolysis method and their yield and properties from different types of biomass from previous research. Shortage of fossil fuels as well as environmental concern has encouraged governments to focus on renewable energy resources. Biomass is regarded as an alternative to replace fossil fuels. There are several thermo-chemical conversion processes used to transform biomass into useful products, however in this review article the focus has been made on liquefaction and pyrolysis method because the liquid obtained which is known as bio-oil is the main interest in this review article. Bio-oil contains hundreds of chemical compound mainly phenol groups which make it suitable to be used as a replacement for fossil fuels.

• 생명과학회지
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• 제18권8호
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• pp.1096-1105
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• 2008

Kentucky bluegrass와 Tall fescue에서는 발아력을 극대화시킬 수 있는 priming 최적 처리 조건은 100mM의 $K_3PO_4$ 용액으로 $25^{\circ}C$에서 1일간 처리였다. Priming 처리된 종자는 무처리에 비해 높은 발아율을 보였고, $T_{50}$과 MDG가 단축되어 신속한 발아를 유도하였다. 반면 NaOH나 KOH는 처리농도가 높아지고 처리기간이 길어질수록 발아력이 급격하게 저하되어 priming 화학제로는 적당하지 않았다. Creeping bentgrass 및 Bentgrass에서는 priming 처리에 관계없이 발아율이 10% 미만으로 낮았다. 그러나 200mM의 $KNO_3$ 로 3일간 priming 처리하면 발아율을 6%까지 향상시킬 수 있었으나, 현저한 수준은 아니었다. Kentucky bluegrass와 Tall fescue에서 priming 처리 기간은 1일 처리가 발아율 향상에 가장 좋았고, 처리 기간이 6일로 길어질수록 발아율이 저하되었고 발아 속도는 지연되었다. 또한 priming 화학제의 처리 농도는 100 mM 처리가 발아력 증진에 좋았으나, 200 mM과 300 mM로 처리농도가 높아지면 발아율이 감소하였다.

• Journal of Ginseng Research
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• 제40권2호
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• pp.127-134
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• 2016

Background: Rhizospheric fungi play an essential role in the plantesoil ecosystem, affecting plant growth and health. In this study, we evaluated the fungal diversity in the rhizosphere soil of 2-yr-old healthy Panax notoginseng cultivated in Wenshan, China. Methods: Culture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng. Results: A total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequenceswas high, accounting for 12.9% and 5.5%, respectively. In total,113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus. Conclusion: Our results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.

• 한국버섯학회지
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• 제8권1호
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• pp.16-21
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• 2010

꽃송이버섯은 다른 버섯에 비해 1.3-${\beta}$-Dglucan 함량이 높은 식용버섯이다. 이 버섯은 면역, 항암효과가 높은 버섯이며, 일본과 한국에서 최근 재배방법이 개발되었다. 하지만 재배방법이 대부분 특허 출원되어있는 실정이다. 그리고 꽃송이버섯의 재배방법이 확립되어있지 않다. 본 연구는 꽃송이버섯의 봉지 단목 재배방법개발하기위해 균사배양 및 원기형성 조건을 조사하였다. 단목 재배에 사용한 수종으로는 낙엽송, 해송, 리기다소나무, 상수리나무 단목을 사용하였고, 리기다소나무 단목에서 자실체 수량이 가장 양호하였다. 그리고 리기다소나무 단목을 생이스트 5%에 8시간 동안 침지하고 생육조건은 온도 및 습도를 각각 90~95%, $23{\sim}25^{\circ}C$처리에서 자실체발생이 가장 양호하였다.

• Plant Biotechnology Reports
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• 제3권2호
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• pp.147-155
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• 2009

Oryza grandiglumis Chitinase IVa (OgChitIVa) cDNA encoding a class IV chitinase was cloned from wild rice (Oryza grandiglumis). OgChitIVa cDNA contains an open reading frame of 867 nucleotides encoding 288 amino acid residues with a predicted molecular weight of 30.4 kDa and isoelectric point of 8.48. Deduced amino acid sequences of OgChitIVa include the signal peptide and chitin-binding domain in the N-terminal domain and conserved catalytic domain. OgChitIVa showed significant similarity at the amino acid level with related monocotyledonous rice and maize chitinase, but low similarity with dicotyledoneous chitinase. Southern blot analysis showed that OgChitIVa genes are present as two copies in the wild rice genome. It was shown that RNA expression of OgChitIVa was induced by defense/stress signaling chemicals, such as jasmonic acid, salicylic acid, and ethephon or cantharidin and endothall or wounding, and yeast extract. It was demonstrated that overexpression of OgChitIVa in Arabidopsis resulted in mild resistance against the fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. RT-PCR analysis showed that PR-1 and PR-2 RNA expression was induced in the transgenic lines. Here, we suggest that a novel OgChitIVa gene may play a role in signal transduction process in defense response against B. cinerea in plants.