• Proceedings of the Korean Society for Bioinformatics Conference
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• 2000.11a
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• pp.13-16
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• 2000

Microorganisms have been widely employed for the production of useful bioproducts including primary metabolites such as ethanol, succinic acid, acetone and butanol, secondary metabolites represented by antibiotics, proteins, polysaccharides, lipids and many others. Since these products can be obtained in small quantities under natural condition, mutation and selection processes have been employed for the improvement of strains. Recently, metabolic engineering strategies have been employed for more efficient production of these bioproducts. Metabolic engineering can be defined as purposeful modification of cellular metabolic pathways by introducing new pathways, deleting or modifying the existing pathways for the enhanced production of a desired product or modified/new product, degradation of xenobiotics, and utilization of inexpensive raw materials. Metabolic flux analysis and metabolic control analysis along with recombinant DNA techniques are three important components in designing optimized metabolic pathways, This powerful technology is being further improved by the genomics, proteomics, metabolomics and bioinformatics. Complete genome sequences are providing us with the possibility of addressing complex biological questions including metabolic control, regulation and flux. In silico analysis of microbial metabolic pathways is possible from the completed genome sequences. Transcriptome analysis by employing ONA chip allows us to examine the global pattern of gene expression at mRNA level. Two dimensional gel electrophoresis of cellular proteins can be used to examine the global proteome content, which provides us with the information on gene expression at protein level. Bioinformatics can help us to understand the results obtained with these new techniques, and further provides us with a wide range of information contained in the genome sequences. The strategies taken in our lab for the production of pharmaceutical proteins, polyhydroxyalkanoate (a family of completely biodegradable polymer), succinic acid and me chemicals by employing metabolic engineering powered by genomics, proteomics, metabolomics and bioinformatics will be presented.

• Journal of Fashion Business
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• v.12 no.6
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• pp.61-72
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• 2008

The UV-protection effect of green-tea dyed fabrics was reported in our previous studies. The chitosan was used as a natural mordant of cellulose fiber for green tea extract because chitosan is a natural bio-polymer. The increase in the UV protection property of summer cellulose fabrics, cotton and linen, upon the repetition of chitosan mordanting and green tea dyeing was observed. However, the physical property change would be followed by this repeated wet processing of the cellulose fabric. Therefore, the physical changes of the chitosan mordanted and green tea dyed cotton and linen fabrics were evaluated by KES-FB system. Tensile, shear, bending, compression, and surface characteristics were tested upon the repetition of mordanting and dyeing treatments. Linearity of tensile force increased in the treated cotton and linen samples. Tensile energy and resilience decreased in all treated fabrics. Shear stiffness increased in the treated cotton and linen in general. Shear hysteresis was increased in all cotton samples and some linen samples. In cotton, the bending rigidity in all treated cottons increased except C3G3. As the chitosan mordanting numbers increased, the bending rigidity tended to decrease. In linen, the bending rigidity and hysteresis increased in all treated samples. Compressional energy and resilience increased as the number of chitosan mordanting increased both in cotton and linen. This could be the result of the increase in thickness upon chitosan mordanting. Surface coefficient of friction increased in the treated cotton and linen in general. Surface roughness tended to increase in cotton.

• Macromolecular Research
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• v.17 no.7
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• pp.499-505
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• 2009

General, fast, and efficient stitching methods are presented for the synthesis of Fr$\acute{e}$chet-type dendrimers with linear units at a core, as a preliminary investigation for the synthesis of dendritic-linear-dendritic materials. The synthetic strategy involved an inexpensive, 1,3-dipolar, cycloaddition reaction between an alkyne and an azide in the presence of the Cu(I) species, which is known as the best example of click chemistry. The linear core building blocks, 1,7-octadiyne and 1,6-diazidohexane, were chosen to serve as the alkyne and azide functionalities for dendrimer growth via click reactions with the azide and alkyne-dendrons, respectively. These two building blocks were employed together with the azide- and alkyne-functionalized Fr$\acute{e}$chet-type dendrons in a convergent strategy to synthesize two kinds of Fr$\acute{e}$chet-type dendrimers with different linear core units. This comparative efficiency of the click methodology supports the fast and efficient synthesis of dendritic-linear-dendritic materials with the tailor made core unit.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.15 no.2
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• pp.1216-1221
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• 2014

Poly(ethylene-co-isosorbide terephthalate) (PEIT) is interest in polymer which has isosorbide monomer that is renewable resources such as corn. However, there is important drawback which is low mechnical properties as increasing isosorbide contents. In this study, polycarbonate used to make up for drawback of mechanical properties of PEIT. In addition, PEIT used to improve the tensile elongation of polycarbonate because PEIT has good sheet proccessability. The effect of polycarbonate on morphology, thermal and mechanical properties were investigated using FE-SEM, DMA, TGA, UTM, and notched izod impact strength tester. As a result of this study, PEIT/PC blends were in compatible system and polycarbonate can act as an improvement of thermal stability and mechanical properties in the blends.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.50-50
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• 2019

최근 국지성 호우 및 하천 제방의 노후화로 인한 제방 붕괴 피해가 빈번히 발생하면서 제방의 안정성 및 표면 보강을 위한 다양한 연구가 시도되고 있다. 본 연구에서는 제방 붕괴에 따른 피해 최소화 및 대책을 수립하기 위한 방법으로 시멘트와 같은 지구온난화를 야기시키는 물질이 아닌 친환경 신소재 바이오폴리머를 흙과 혼합한 재료를 활용하여 제방의 내구성을 강화하기 위한 연구가 수행되고 있다. 이에 안동하천실증연구센터에서는 현장토를 사용하여 높이 1 m, 폭 3 m, 사면경사 1 : 2, 총 길이 5 m 의 중규모 제방모형을 제작하였으며, 공동연구기관인 카이스트에서 개발된 바이오폴리머와 흙을 적정 비율로 혼합한 바이오-소일을 제방 전면에 일정 두께로 피복하여 월류 발생에 따른 제방 안정성 평가 실험을 수행하였다. 1차 실험은 흙 제방 조건이며, 2 3차 실험은 제방 표면에 5 cm 두께로 신소재가 피복된 조건으로 안정된 결과 도출을 위해 반복 실험을 수행하였다. 제방 천단면 및 사면에서의 유속분포를 측정하기 위해 드론 및 비디오카메라를 활용한 LSPIV 기법을 적용하여 실험조건에 따른 표면유속과 월류 흐름이 제방 붕괴에 미치는 영향에 대해 비교분석하였다. 또한 그래픽 소프트웨어를 이용한 픽셀기반 영상분석 기법을 적용하여 시간에 따른 제방사면의 붕괴면적을 산정하여 신소재 피복에 따른 붕괴 지연효과 분석을 통한 신소재 활용 제방의 현장 적용가능성 및 안정성을 평가하였다. 본 연구결과, 흙 제방의 경우 월류 흐름 발생 직후 침식현상이 전개되어 유속분포가 집중되고 있었으며, 이후 발생하는 강한 수직흐름으로 인해 입자추적을 통한 분석이 더 이상 불가능하였다. 신소재 제방의 경우 월류 흐름 발생 직후 침식은 발생하지 않았으며 일정시간동안 유속분포가 유지되었다. 지속적인 월류 흐름으로 인해 제방 끝단에서 침식이 발생하였으며 이때 최대 유속은 3.3 m/s 로 나타났다. 또한 픽셀기반 분석을 통해 30초 단위로 표면손실률을 산정한 결과, 신소재 활용 제방(2, 3차)의 경우 같은 실험조건에도 불구하고 최종 붕괴시간은 약 3배의 차이를 보였으며, 양생 과정에서의 크랙 발생을 최소화한다면 월류 발생 시 상당한 붕괴지연효과가 있는 것으로 판단된다.

• Ecology and Resilient Infrastructure
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• v.6 no.2
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• pp.120-127
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• 2019

Medium-scale levee experiments were performed to monitor the infiltration and failure of levee body by applying fiber optic temperature sensing. In this study, bio-polymer soil was spread in the levee slope to increase the strength and intensity. Therefore, the infiltration and failure by overflows were produced in a different way compared to general soil type of levees. This was also observed in the experiment data for temperature changes monitored by fiber-optic distributed temperature sensing system. Through the analysis of temperature changes at specific location by time, the location and initiation time for physical changes and infiltration in levee body could be identified based on temperature variation. In this experiment, the time of rapid changes in temperature was ahead in the inland slope rather than the forceland slope. It was corresponding to the levee failure sequence of first inland slope failure and then the forceland slope failure.

• Journal of Microbiology and Biotechnology
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• v.29 no.5
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• pp.776-784
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• 2019

Polyhydroxybutyrate (PHB), the most well-known polyhydroxyalkanoate, is a bio-based, biodegradable polymer that has the potential to replace petroleum-based plastics. Lignocellulose hydrolysate, a non-edible resource, is a promising substrate for the sustainable, fermentative production of PHB. However, its application is limited by the generation of inhibitors during the pretreatment processes. In this study, we investigated the feasibility of PHB production in E. coli in the presence of inhibitors found in lignocellulose hydrolysates. Our results show that the introduction of PHB synthetic genes (bktB, phaB, and phaC from Ralstonia eutropha H16) improved cell growth in the presence of the inhibitors such as furfural, 4-hydroxybenzaldehyde, and vanillin, suggesting that PHB synthetic genes confer resistance to these inhibitors. In addition, increased PHB production was observed in the presence of furfural as opposed to the absence of furfural, suggesting that this compound could be used to stimulate PHB production. Our findings indicate that PHB production using lignocellulose hydrolysates in recombinant E. coli could be an innovative strategy for cost-effective PHB production, and PHB could be a good target product from lignocellulose hydrolysates, especially glucose.

• Membrane Journal
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• v.31 no.4
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• pp.241-252
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• 2021

Rapid industrialization with growing population leads to environmental water pollution. Demand in generation of clean water from waste water is ever increasing by scarcity of rain water due to change in weather pattern. Colorimetric detection of heavy metal present in clean water is very simple and effective technique. In this review membrane based colorimetric detection of mercury (II) ions are discussed in details. Membrane such as cellulose, polycaprolactone, chitosan, polysulfone etc., are used as support for metal ion detection. Nanofiber based materials have wide range of applications in energy, environment and biomedical research. Membranes made up of nanofiber consist up plenty of functional groups available in the polymer along with large surface area and high porosity. As a result, it is easy for surface modification and grafting of ligand on the fiber surface enhanced nanoparticles attachment.

• Membrane Journal
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• v.31 no.6
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• pp.393-403
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• 2021

Enzymes are important class of catalyst for biotransformation. Stability and reusability of enzymes during the catalysis process is a key issue. Activity of enzyme can be enhanced by its immobilization on a suitable substrate by creation of specific microenvironment. A variety of membranes has been used as substrate due to the biocompatibility and simpler method to tune hydrophilicity/hydrophobicity property of the membrane surface. In this review, polymer membranes including cellulose, polyacrylonitrile (PAN), polydimethylsiloxane (PDMS), polyvinylidene fluoride (PVDF), polyethersulfone (PES) are introduced and discussed in detail. Biodegradation of organic contaminants by immobilized enzyme is an environmental friendly process to reduce the contamination of environment in pharmaceutical company and textile industries. The controlled hydrolysis of oil can be performed in enzyme immobilized membrane bioreactor (EMBR), resulting in reducing carbon emission and reduced environmental pollution. Bioethanol and biodiesel are considered alternative fossil fuels that can be prepared in EMBR.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.220-220
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• 2021

2020년 장마는 6월 중순부터 8월 중순까지 전국적으로 평균 687 mm의 강수가 내려, 1973년 이후 역대 2위 강수량을 기록하였으며, 연이은 태풍으로 큰 인명 및 재산 피해가 발생하였다. 특히, 섬진강 및 한탄천 등에서 계획홍수위를 초과하는 홍수로 인해 상당수의 제방이 월류로 인해 붕괴된 것으로 나타났다. 따라서, 향후 기후변화에 따른 연평균 강수량이 증가할 것으로 전망되는 가운데 집중호우로 인한 제방 붕괴 피해를 최소화하기 위한 고도화된 기술 개발을 통한 선제적 재발 방지대책이 필요한 시점이다. 한국건설기술연구원은 바이오폴리머라는 새로운 친환경 신소재를 이용하여 제방의 안정성 평가 기술 개발 연구를 수행하고 있다. 이에 안동하천연구센터에서는 실규모에 준하는 제방모형(높이 3 m, 사면경사 1:2, 길이 10 m 이상)을 제작하고, 제방 표면에 바이오폴리머 신소재를 처리하여 전방 월류 흐름 유도에 따른 실규모 제방붕괴실험을 수행하였다. 또한, 신소재 보강 및 무보강 조건에 따른 영상분석 기반 붕괴지연효과를 정량적으로 분석하여 신소재의 성능을 평가하였다. 하지만, 기존에 수행된 실험은 댐 붕괴 흐름과 같이 홍수파가 발생하여 제내지로 퍼져 나가는 형태로 진행되어, 보강공법의 검증에 있어 실제 하천에서 발생하는 횡월류 흐름을 재현하지 못한다는 한계를 가지고 있다. 본 연구에서는 횡월류 흐름(0.6 m³/s 이상)을 발생시켜 수리실험에 따른 축척효과(scale effect)를 최소화하고, 현장에 대한 충분한 자연성을 재현하는 것을 목표로 하여 실험을 수행하였다. 실험 조건은 1) 신소재가 처리된 식생 제방, 2) 신소재가 처리되지 않은 식생 제방으로 각각의 조건에 따른 횡월류 흐름 및 제방 붕괴를 유도하여 영상분석 기법(이미지 픽셀분석 및 3D 포인트 클라우드 모델링)을 통한 침식 저항에 관한 분석결과를 제시하였다.