• Bulletin of the Korean Chemical Society
• /
• v.32 no.4
• /
• pp.1415-1418
• /
• 2011

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.1015-1016
• /
• 2005

• Bulletin of the Korean Chemical Society
• /
• v.30 no.12
• /
• pp.3137-3140
• /
• 2009

• Bulletin of the Korean Chemical Society
• /
• v.29 no.1
• /
• pp.228-230
• /
• 2008

• Proceedings of the Korean Environmental Sciences Society Conference
• /
• 2017.10a
• /
• pp.212-212
• /
• 2017

• 한국생물공학회:학술대회논문집
• /
• 2005.10a
• /
• pp.1010-1010
• /
• 2005

• Journal of Microbiology and Biotechnology
• /
• v.17 no.10
• /
• pp.1607-1615
• /
• 2007

We investigated the applicability of the TEM-l ${\beta}$-lactamase fragment complementation (BFC) system to develop a strategy for the screening of protein-protein interactions in bacteria. A BFC system containing a human Fas-associated death domain (hFADD) and human Fas death domain (hFasDD) was generated. The hFADD-hFasDD interaction was verified by cell survivability in ampicillin-containing medium and the colorimetric change of nitrocefin. It was also confirmed by His pull-down assay using cell lysates obtained in selection steps. A coiled-coil helix coiled-coil domain-containing protein 5 (CHCH5) was identified as an interacting protein of human uracil DNA glycosylase (hUNG) from the bacterial BFC cDNA library strategy. The interaction between hUNG and CHCH5 was further confirmed with immunoprecipitation using a mammalian expression system. CHCH5 enhanced the DNA glycosylase activity of hUNG to remove uracil from DNA duplexes containing a U/G mismatch pair. These results suggest that the bacterial BFC cDNA library strategy can be effectively used to identify interacting protein pairs.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.7
• /
• pp.709-712
• /
• 2009

Enzymatic glucosylation with glycosyltransferases can be used to regulate the water solubility of aglycone. The drawback of this process is the demand of UDP-glucose as a sugar donor. We made an in-frame fusion of the flavonoid O-glucosyltransferase (OsUGT-3) and sucrose synthase (AtSUS) genes. The resulting fusion protein, OsUGT3-AtSUS, was expressed in E. coli and purified. When sucrose and UDP were supplied, the fusion protein was able to convert quercetin into quercetin O-glucoside without the addition of UDP-glucose. In addition, UDP-glucose was recycled when sucrose was added to the reaction mixture. This fusion protein is useful for the enzymatic production of flavonoid O-glucosides.

• Bulletin of the Korean Chemical Society
• /
• v.27 no.2
• /
• pp.281-285
• /
• 2006

Microbial fuel cells (MFC) stacked with bipolar plates have been constructed and their performance was tested. In this design, single fuel cell unit was connected in series by bipolar plates where an anode and a cathode were made in one graphite block. Two types of bipolar plate stacked MFCs were constructed. Both utilized the same glucose oxidation reaction catalyzed by Gram negative bacteria, Proteus vulgaris as a biocatalyst in an anodic compartment, but two different cathodic reactions were employed: One with ferricyanide reduction and the other with oxygen reduction reactions. In both cases, the total voltage was the mathematical sum of individual fuel cells and no degradation in performance was found. Electricity from these MFCs was stored in a supercapacitor to drive external loads such as a motor and electric bulb.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.1
• /
• pp.188-196
• /
• 2013

We have investigated the effects of formalin on the assembly of colloidal gold nanoparticles (AuNPs) prepared by laser ablation of a solid gold target in deionized water. Upon addition of formalin, the surface plasmon resonance (SPR) band at 519 nm for pure AuNPs decreases and shifts to red while a new broad SPR band appears at ~700 nm. The red-shift is prominent with increase in the incubation time. The average size of the initial AuNPs is around 12 nm but it increases to 23 nm after addition of formalin. It turns out that formalin acts as a cationic surfactant for AuNPs with negative surface charge in the colloidal solutions. Furthermore, through analysis of the Raman spectrum of formalin and the density functional theory calculations, we confirm that methanediol is the main species in formalin which is in charge of the aggregation of AuNPs.