• International Journal of Stem Cells
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• v.17 no.2
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• pp.194-203
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• 2024

Evaluating cell metabolism is crucial during pluripotent stem cell (PSC) differentiation and somatic cell reprogramming as it affects cell fate. As cultured stem cells are heterogeneous, a comparative analysis of relative metabolism using existing metabolic analysis methods is difficult, resulting in inaccuracies. In this study, we measured human PSC basal metabolic levels using a Seahorse analyzer. We used fibroblasts, human induced PSCs, and human embryonic stem cells to monitor changes in basal metabolic levels according to cell number and determine the number of cells suitable for analysis. We evaluated normalization methods using glucose and selected the most suitable for the metabolic analysis of heterogeneous PSCs during the reprogramming stage. The response of fibroblasts to glucose increased with starvation time, with oxygen consumption rate and extracellular acidification rate responding most effectively to glucose 4 hours after starvation and declining after 5 hours of starvation. Fibroblasts and PSCs achieved appropriate responses to glucose without damaging their metabolism 2~4 and 2~3 hours after starvation, respectively. We developed a novel method for comparing basal metabolic rates of fibroblasts and PSCs, focusing on quantitative analysis of glycolysis and oxidative phosphorylation using glucose without enzyme inhibitors. This protocol enables efficient comparison of energy metabolism among cell types, including undifferentiated PSCs, differentiated cells, and cells undergoing cellular reprogramming, and addresses critical issues, such as differences in basal metabolic levels and sensitivity to normalization, providing valuable insights into cellular energetics.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.9
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• pp.933-938
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• 2008

In the present study, carbon dioxide was converted to methane, using bio-hydrogen. Here, the bio-hydrogen was produced from organic waste. The anaerobic microorganism was cultured using only carbon dioxide and hydrogen for duration of 3 months. Therefore methane was not produced with acetogenotrophs. During methane production, carbon dioxide and hydrogen are taken in different ratios; among which 1 : 5 ratio has shown the highest methane yield. Carbon dioxide and hydrogen were introduced into the reactor at the rate of 8 mL/min and 40 mL/min, respectively. In this case, 92% of carbon dioxide was reduced and 2.2 m$^3$/m$^3$ day amount of methane was produced. Thus, the process has been successful in conversion of carbon dioxide into methane by purging it into methane fermentation reactor with bio-hydrogen using batch process.

• Journal of the Korean Society of Industry Convergence
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• v.21 no.6
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• pp.337-344
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• 2018

Food irradiation is important not only in ensuring safety but also improving antioxidant activity of peaches. Our objective was to establish the best irradiation treatment for peaches by calculating dose distribution using Monte Carlo simulation. 3-D geometry and component densities of peaches, extracted from CT scan, were entered into MCNP to obtain simulated dose distribution. Radiation energies for electron beam were 1.35 MeV (low energy) and 10 MeV (high energy). Co (1.25 MeV) and the Husman irradiator, containing three sealed Cs source rods in an annular array, were used for gamma irradiation. At 1.35 MeV electron beam simulation, electrons penetrated well beyond the peach skin, enough for surface treatment for microorganisms and allergens. At 10 MeV electron beam simulation, for top-beam only treatment, doses at the core were the highest and for double beam treatment, the electron energy was absorbed by the entire sample. At Co source, the radiation doses were presented on the whole area. At Cs source, the dose uniformity ratios were 2.78 for one source and 1.48 for three ones at 120 degrees interval. Proper control of irradiation treatment is critical to establish confidence in the irradiation process.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.26-26
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• 2012

The hydrophobic nature of the wool surface give rise to difficult penetration of dye molecules. Among all the methods of modification, graft polymerization is an attractive method to impart a variety of functional groups to a polymer. Grafting has been made by irradiating the light on the polymer in the presence of a solvent containing monomer. The energy source commonly used are high-energy electrons, X-rays, UV and visible light. UV irradiation is a relatively low-energy radiation in comparison with others since it has the least possibility to change bulk properties. In the present paper, a photo-reactive dye was synthesized from quinizarin by the reaction with methacryloyl chloride. The synthesized dye was continuously grafted onto wool fabric at room temperature by UV irradiation. Several key parameters including UV energy, dye concentration and pH have been examined to understand their influence on the photoreactive coloration.

• Textile Coloration and Finishing
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• v.21 no.5
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• pp.16-20
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• 2009

Films of Poly(ethylene oxide), PEO, were modified to impart hydrophilicity via UV irradiation. The UV irradiation treatment produced new photo-oxidized groups of carbonyl and ether groups as indicated in ATR and ESCA analysis. It was found that water contact angle decreased from $15^{\circ}C$ to $10^{\circ}C$ and total surface energy of PEO increased from 54.2 mN/m to 76.6 mN/m with increasing UV energy, which was attributed to significant contribution of acid base interaction of the photo-oxidized PEO rather than nonpolar interaction originating from the dominant increase in Lewis acid parameter. The increased hydrophilicity and surface energy were also proved by the decreased water wetting time.

• Proceedings of the Membrane Society of Korea Conference
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• 2005.11a
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• pp.119-123
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• 2005

The thermochemical splitting of water has been proposed as a clean method for hydrogen production. The IS process is one of the thermochemical water splitting processes using iodine and sulfur as reaction agents. HI decomposition procedure to obtain hydrogen is one of the key operations in the process, because equilibrium conversion of HI is low (22% at $450^{\circ}C$). The silica membranes prepared by CVD. method were applied to the decomposition reaction of HI vapor. The permeation characteristics of hydrogen and nitrogen belong to the Knudsen flow pattern.

• Journal of Biosystems Engineering
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• v.37 no.6
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• pp.429-433
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• 2012

Purpose: While rapeseed oil, soy bean oil, palm oil and waste cooking oil are being used for biodiesel, the viscosity of them should be lowered for fuel. The most widely used method of decreasing the viscosity of vegetable oil is to convert the vegetable oil into fatty acid methyl ester but is too expensive. This experiment uses ultrasonic energy, instead of converting the vegetable oil into fatty acid methyl ester, to lower the viscosity of the waste cooking oil. Methods: For irradiation treatment, the sample in a beaker was irradiated with ultrasonic energy and the viscosity and temperature were measured with a viscometer. For heating treatment, the sample in a beaker was heated and the viscosity and temperature were measured with a viscometer. Kinematic viscosity was calculated by dividing absolute viscosity with density. Results: The kinematic viscosity of waste cooking oil and cooking oil are up to ten times as high as that of light oil at room temperature. However, the difference of two types of oil decreased by four times as the temperature increased over $83^{\circ}C$. When the viscosity by the treatment of ultrasonic energy irradiation was compared to one by the heating treatment to the waste cooking oil, the viscosity by the treatment of ultrasonic energy irradiation was lower by maximum of 22% and minimum of 12%, than one by the heating treatment. Conclusions: Ultrasonic energy irradiation lowered the viscosity more than the heating treatment did, and ultrasonic energy irradiation has an enormous effect on fuel reforming.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.22 no.1
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• pp.45-56
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• 2018

The authors are using Qi-diagnosis (integrated diagnosis by bio-energy) that is a method of diagnosis and treatment. We applied Qi-diagnosis to the main study to lay the foundation and framework for research and education about the Korean Medicine. The authors try to describe systemically and specifically the Qi-diagnosis that the authors are using in clinical diagnosis ane treatment so that anyone can use it. The authors have been able to grasp the flow of human bio-energy through years of training. It has had many effects by applying the Qi-diagnosis to patients. The steps of the bio-energy flow have become objective. And the authors have been applied to acupuncture, herbal medicine, moxibustion, bruising treatment and anthrax anesthesia in clinical through the Qi-diagnosis. Also, it is applied to the life management of patients. It is applied to arts such as music therapy and art therapy. The deeper the depth of the Qi-diagnosis, the greater the opportunity to utilize the Qi-diagnosis. The Qi-diagnosis is the origin of the korean medicine. It was able to make diagnosis and treatment correct and to establish clues that the medical problems would be solved through the Qi-diagnosis. In order to do so, the diagnostician must be able to feel the auricular flow of the body accurately and objectively. In addition, he must have a comprehensive understanding of the overall framework of medicine. As a result, diagnosis and treatment of the patient as well as general problems of the patient can be identified and advised, so comprehensive treatment is possible. And it is not only a specific person can do it, but it is a diagnostic method that anyone can take if they take the basic steps step by step.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.32 no.8
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• pp.582-588
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• 2008

An experimental investigation was conducted to investigate the effect of Bio-ethanol fuel on the engine performance and exhaust emission characteristics under various engine operating conditions. To investigate the effect of bio-ethanol fuel, the commercial 1.6L SI engine equipped with 4 cylinder was tested on EC dynamometer. The engine performance including brake torque, brake specific fuel consumption, and barke specific energy consumption of bio-ethanol fuel was compared to those obtained by pure gasoline. Furthermore, the exhaust emissions were analyzed in terms of regulated exhaust emissions such as unburned hydrocarbon, oxides of nitrogen, and carbon monoxide.Result of this work shows that the effect of blending of ethanol to gasoline caused drastic decrease of emissions under various operating conditions. Also, improved engine performance such as brake torque and brake power were indicated for bio-ethanol fuel.

• Proceedings of the KIEE Conference
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• 2002.07b
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• pp.1379-1381
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• 2002

Recently, environmental pollution by livestock excretion is gathering strength as livestock industry is advanced on a large scale. Bio-gas is generated more than about 70 precent if remove impurities in the course of extracting bio-gas to use livestock excretion. In this paper, we developed a device that can use it in heating after extracting bio-gas from livestock excretion collected in livestock farmhouse and can supply electricity by using generator developed in itself. Also, because this system is epoch-making no discharge system to solve smear of existing method, it is expected that we have effects to prevent environmental pollution and get alternation energy and burden on the institution to treat livestock excretion of a pig breeder is solved, if use it.