• 생물환경조절학회지
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• 제6권4호
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• pp.277-283
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• 1997

본 실험은 초화류인 코레우스의 삽목시 삽수의 발근율을 높이기 위하여 배양액과 NAA 및 IBA처리 효과를 조사하였다. 모래삽목보다는 배양액을 이용한 것이 발근소요일이나 생육면에서 월등히 효과적이었다. 특히 배양액 농도는 일본 원시표준액보다는 표준액의 1/4 배양액 농도에서 재배한 것이 가장 발근이 빨리 되었으므로 삽목초기에는 낮은 양액농도가 경제적일 것으로 보인다. 생장조절제의 농도별로는 NAA나 IBA 0.01mg/$\ell$처리가 효과적이었으며 IBA처리보다는 NAA처리시의 발근소요일이 더 짧았다. 또한 NAA 및 IBA을 처리한 수돗물을 사웅하여 삽수의 발근을 유도할 경우 NAA와 IBA를 삽수에 침지처리한 후 수돗물에 재배하는 것보다 발근에 효과적이었으며, 무엽지삽보다는 유엽지삽이 효과적이었다. 또한 배양액이나 생장조절제를 단용처리한 것보다 배양액과 생장조절물질의 혼용처리가 발근소요일이 단축되었고 생육상태가 양호하였다.

• Clinical and Experimental Reproductive Medicine
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• 제25권3호
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• pp.245-249
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• 1998

White blood cells (WBCs) are present in most human ejaculates, but abnormally high concentration of seminal leukocytes may reflect an underlying pathological condition. The World Health Organization (WHO) has defined leukocytospermia as status of more than $10^6$ WBC/mL of semen. The purpose of this study was firstly, to compare the outcomes between conventional IVF and ICSI in leukocytospermia, and secondly, to investigate whether ICSI may be an alternative treatment for patients with leukocytospermia. Total 121 cycles of conventional IVF and ICSI candidates underwent IVF cycles at PL Infertility Clinic. Semen Parameters including concentration, motility, morphology of spermatozoa and concentration of leukocytes were assessed from the raw ejaculates. There was no difference in sperm concentration, motility and morphology. The rates of fertilization and good embryo development from ICSI were significantly higher than those from conventional IVF in leukocytospermia (63.9% & 48.6%, respectively for ICSI group and 33.4% & 24.1%, respectively for IVF group, p<0.001). The pregnancy rate after ICSI was also higher than that from conventional IVF (34.3% vs 21.6%, p<0.05). These results indicate that the presence of seminal leukocytes ($>1\times10^6$ WBC/mL of semen) is adversely related with fertilization, embryo development and pregnancy rate. Therefore the measurement of seminal leukocytes in routine semen analysis appears to be of prognostic value with regard to male fertilizing potential. In conclusion, it is suggested that ICSI is an alternative choice of treatment for patients with leukocytospermia.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.751-756
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• 1999

For mass production of poly(3-hydroxybutyrate) (PHB), high cell density cultures of Ralstonia eutropha were carried out in 2.5-1 and 60-1 fermentors by two fed-batch culture techniques of nitrogen and phosphate limitation. When the nitrogen limitation technique was employed using both an on-line glucose monitoring and control system, a high concentration level of PHB (121g/l) was obtained in the small-scale fermentor of 2.5 1. However, the PHB concentration obtained in a large-scale fermentor of 60 1 only turned out to be 60g/l. In contrast, when another fed-batch culture technique of the phosphate-limitation employing dissolved oxygen (DO) stat glucose feeding was used, a large amount of PHB was successfully produced in both 60-1 and 2.5-1 fermentors. In a 2.5-1 fermentor, concentrations of PHB and cells obtained in 58 h were 175 and 210 g/l, respectively, which corresponded to the PHB productivity level of 3.02 g/l/h. In a 60-1 fermentor, a final cell concentration of 221 g/l and a PHB concentration of 180 g/l with PHB productivity level of 3.75 g/l/h were obtained in 48h. PHB content and yield from glucose were 81% and 0.38g PHB/g glucose, respectively. These data suggest that the phosphate limitation technique is more effective compared to nitrogen limitation in the mass production of PHB by R. eutropha of a large scale.

• 한국수정란이식학회지
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• 제25권4호
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• pp.251-258
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• 2010

In the present study, effects of concentration and time of culture in presence of roscovitine on nuclear maturation and meiotic spindle configuration, chromosomal alignment were examined in porcine oocytes. In experiment 1, porcine cumulus oocyte complexes (COCs) were cultured at $39^{\circ}C$ in a 5% $CO_2$ atmosphere in North Carolina State University 23 (NCSU-23) supplemented with 25, 50, 75 or $100\;{\mu}M$ roscovitine for 22 h and then were cultured for additional 22 h after removal of roscovitine. Nuclear maturation and morphology of the meiotic spindle and chromosomal alignment were examined to determine the optimal concentration of roscovitine in oocyte maturation. In experiment 2, COCs were cultured in NCSU-23 supplemented with $50\;{\mu}M$ roscovitine for 17, 20, 27 or 42 h and then an additional 22 h without roscovitine was followed to determine the optimal time of culture. The optimal concentration of roscovitine to arrest and resume meiosis of porcine oocyte was $50\;{\mu}M$ by examining nuclear status (p<0.05) and normal spindle and chromosome configuration. The optimal time of culture in presence of roscovitine to arrest meiosis of porcine oocyte was 17 h (p<0.05), although MII rates and normal morphology of the meiotic spindle and chromosomal alignment were not significantly different among various times of culture. In conclusion, the optimal concentration and time of culture in presence of roscovitine to arrest porcine oocytes are $50\;{\mu}M$ and 17 h, respectively.

• Journal of Pharmaceutical Investigation
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• 제41권3호
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• pp.153-159
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• 2011

This study was designed to investigate the effects of silibinin on the pharmacokinetics of carvedilol after oral administration of carvedilol in rats. Carvedilol was administered orally (3 mg/kg) with oral silibinin (0.3, 1.5 or 6 mg/kg) and intravenously (1 mg/kg) to rats. The effects of silibinin on P-glycoprotein (P-gp) and cytochrome P450 (CYP) 2C9 and CYP2D6 activity were also evaluated. Silibinin inhibited CYP2C9 and CYP2D6 enzyme activity with 50% inhibition concentration ($IC_{50}$) of 5.2 ${\mu}M$ and 85.4 ${\mu}M$, respectively. In addition, silibinin significantly enhanced the cellular accumulation of rhodamine-123 in MCF-7/ADR cells overexpressing P-gp. Compared with the control group, the area under the plasma concentration-time curve was significantly increased by 36.3-57.1%, and the peak concentration was significantly increased by 51.1-88.5% in the presence of silibinin after oral administration of carvedilol. Consequently, the relative bio-availability of carvedilol was increased by 1.13- to 1.57-fold and the absolute bioavailability was significantly increased by 38.6-59.7%. The time to reach peak concentration and the terminal half-life were not significant. The enhanced oral bio-availability of carvedilol may result from inhibition of CYP2C9-mediated metabolism and P-gp-mediated efflux of carvedilol rather than inhibition of CYP2D6-mediated metabolism in the intestine and/or in the liver by silibinin.

• 생물환경조절학회지
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• 제29권2호
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• pp.181-188
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• 2020

본 연구는 국내 특산식물이며 관상소재로 개발이 가능한 산꼬리풀의 효과적인 육묘조건을 구명하기 위해 수행되었다. 육묘는 토양 종류와 비료 농도, 차광정도, 추비 농도, 종자의 광원 전처리 및 채종 시기별로 각 요인을 달리하여 수행하였다. 연구 결과, 산꼬리풀은 원예상토에서 생육이 우수하였으며, 혼용토에서는 비료 첨가 시 생육이 억제되었다. 차광은 55% 차광조건에서 무차광에 비해 전체적인 생육이 향상되었다. 고농도(1000mg·L^-1)의 Hyponex는 육묘 시 생육을 촉진하였으며, 파종 전 종자에 대한 적색광 조사는 추후 초장과 생체중 향상에 효과적이었다. 채종 시기는 2018년도 종자가 2017년 종자보다 생육이 우수하였다.

• 지역연구
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• 제39권2호
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• pp.19-30
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• 2023

본 연구는 공간적 범위가 다른 지역 간 개발격차를 파악하기 위해 산업조직론의 집중지수를 활용하여 실증적으로 분석하고 이를 통한 정책적 시사점 제안한다. 연구의 범위는 2000년대부터 현재까지 지속적으로 해당 산업을 육성해 온 충청북도 11개 시·군이며, 선행연구 고찰을 통해 지역개발격차지수 이론을 기반으로 상위기업집중률과 허핀달 지수를 이용하여 분석하였다. 연구대상은 전국 지자체가 경쟁적으로 육성 의지를 표명하고 있는 바이오헬스산업으로 대상지의 시·군경쟁력강화산업 결과를 기반으로 4개의 세부분야(제약, 의료기기, 건강기능식품, 화장품)로 나누어 연구를 수행하였다. 세부분야별 매출액, 종사자수, 연구개발비를 기준으로 분석한 지역개발격차지수 결과에 따라 지역별로 강점이 다른 것을 확인할 수 있었으며, 이 결과를 통해 향후 지역별 차별화된 산업육성 전략을 수립하는 데 기초자료로 활용될 수 있는 정책적 시사점을 도출하였다.

• 한국고분자학회지:고분자과학과기술
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• 제24권3호
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• pp.254-260
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• 2013

• 상하수도학회지
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• 제26권6호
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• pp.797-805
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• 2012

Contribution of immobilized media with bacteria to the odor removal was evaluated in a lab scale bio-filter compared to that with sponge or ceramic media without the immobilized bacteria. Candida tropicalis for volatile organic compounds and ammonium oxidizing bacteria (AOB) for inorganic compounds were used as seeds in lab-scale bio-reactors. Three different type of media in the bio-reactors that immobilized bioreactor (IBR), sponge bioreactor (SBR), and ceramic bioreactor (CBR) were examined, respectively. An empty bed contact time (EBCT) of the bio-filters was fixed as 60 seconds, and the inlet concentration of toluene was changed from 20 ppm to 200 ppm to observe the removal efficiency depending on the concentrations. As a result, the maximum elimination capacities of IBR, SBR, and CBR were 166 $g/m^3/hr$, 138 $g/m^3/hr$, and 138 $g/m^3/hr$, respectively. In addition, toluene as an organic compound and ammonia as an inorganic compound were applied together with different inlet concentrations varied from 80 ppm to 250 ppm of toluene and from 2.5 ppm to 40 ppm of ammonia. The toluene maximum elimination capacities in IBR, SBR, and CBR were 97.4 $g/m^3/hr$, 59.5 $g/m^3/hr$, and 81.9 $g/m^3/hr$, respectively. The ammonia maximum elimination capacities were reached as 7.2 $g/m^3/hr$ in IBR, 6.6 $g/m^3/hr$ in SBR, and 7.0 $g/m^3/hr$ in CBR.

• 한국통신학회논문지
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• 제35권2B호
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• pp.232-243
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• 2010

본 논문에서는 다양한 생체신호들을 이용하여 심리상태와 생체정보를 판별하고, 외부환경 정보와 함께 이용자의 현재 상황을 인식하여 그에 맞는 적절한 서비스를 제공하는 생체정보기반 상황인식시스템(Bio-Signal Context aware system :BSC)을 설계하고 구현한다. 본 논문에서 구현한 생체정보기반 상황인식시스템은 센서를 통하여 측정된 뇌파(EEG), 심전도(ECG), 피부전도도(GSR) 등의 생체신호들로부터 특징들을 추출하고 분석하였으며, 분석된 결과를 입력받아 평온, 집중, 긴장, 우울의 네 가지 심리상태를 판별하였다. 판별된 심리상태의 결과와 함께 심박변이도(HRV), 피부전도도, 체온 등의 생체신호로부터 분석된 생체 상황정보, 그리고 외부 환경의 상황정보로부터 이용자의 현재 상황을 추론하고 인식하여 현재 생체 상황에 맞는 적절한 서비스를 제공하였다.