• Title/Summary/Keyword: Bio-Conversion

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Effect of crude protein content and undegraded intake protein level on productivity, blood metabolites, carcass characteristics, and production economics of Hanwoo steers

  • Lee, Youn Hee;Ahmadi, Farhad;Lee, Myun;Oh, Young-Kyoon;Kwak, Wan Sup
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.10
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    • pp.1599-1609
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    • 2020
  • Objective: This study was designed to determine how feeding diets differing in crude protein (CP) and undegraded intake protein (UIP) levels affected productivity, blood metabolites, carcass characteristics, and the production economics of Hanwoo steers. Methods: Thirty-six Hanwoo steers (age = 8.2±0.5 mo; body weight = 254±16.1 kg) were assigned at random to one of three treatments (4 steers/pen; 3 pens/treatment): i) a low-CP diet (LP; control) containing 12.1% CP with 35.1% UIP, 12.0% CP with 36.8% UIP, and 12.9% CP with 48.8% UIP, in the growing, fattening, and finishing periods, respectively; ii) a high-CP, low-UIP diet (HPLU) containing 15.0% CP with 33.7% UIP, 14.0% CP with 35.7% UIP, and 13.1% CP with 46.7% UIP, respectively; and iii) a high-CP, high-UIP diet (HPHU) containing 15.0% CP with 45.8% UIP, 14.0% CP with 44.6% UIP, and 13.0% CP with 51.1% UIP, respectively. Results: The treatments did not affect feed intake and growth performance, except for average daily gain during the fattening period that tended to be the lowest (p = 0.08) in the HPLU-fed steers. The feed CP conversion ratio over the entire feeding period was higher with high-CP diets. The treatments did not affect most blood metabolites; however, blood cholesterol and low-density lipoprotein concentrations during the fattening and finishing periods were the lowest in steers fed a HPLU diet. The treatments had negligible effects on cold carcass weight, yield traits including longissimus muscle area, backfat thickness, yield index, and yield grade, plus quality traits including meat color, fat color, texture, and maturity. However, marbling score and frequency of carcass quality grade 1++ were greater in HPHU-fed steers. Conclusion: Feeding diets with higher CP and UIP levels did not affect growth performance but tended to improve the carcass quality of Hanwoo steers, resulting in greater economic return.

Effect of Dietary Astaxanthin Producing Bacteria (Xanthophyllomyces dendrohous) on the Growth Performance and the Meat Quality of Ducks (아스타잔틴 생성 균주(Xanthophyllomyces dendrohous)의 급여가 오리의 성장과 육질에 미치는 영향)

  • Kim, Kyeong-Su;Lee, Min-Kyoung;Lee, Woo-Jin;Choi, Yang-Il;Cho, Seong-Ku
    • Journal of Animal Science and Technology
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    • v.53 no.2
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    • pp.139-146
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    • 2011
  • This study was carried out to investigated astaxanthin producing "Xanthophyllomyces dendrohous" on growth performances and meat quality in ducks. A total of 450 ducks (cheribery) were allotted into 3 groups. The three groups were control (commercial feed), treatment 1 (0.1% feed additives), and treatment 2 (0.2% feed additives). Each group had 3 replicates. Viable cell number of "Xanthophyllomyces dendrohous" is $1.0{\times}10^8$ cfu/g. Growth performance carried out during 39 days. Average weight gain was significantly higher (p<0.05) in treatment 2 than that of control. Feed conversion was significantly lower (p<0.05) in treatment 1 and 2 than that of control. The results of nutrients composition analysis of duck meat showed that treatment 1 and 2 had significantly lower (p<0.05) fat and cholesterol levels. Water holding capacity showed significantly higher (p<0.05) value than that of control. Both treatment groups showed lower (p<0.05) value than control in drip loss and shear force. Control showed higher unsaturated fatty acids(palmitic acid, stearic acid) content than treatment 1 and 2. Treatment 1 and 2 showed significantly lower (p<0.05) saturated fatty acids (oleic acid, linoleic acid) levels than control. These results suggested that the supplementation of feed additives containing "Xanthophyllomyces dendrohous" might be used effectively for inproving productivity and meat quality of ducks.

Increase of Functional Saponin by Acidic Treatemnt and Temperature of Red Ginseng Extract (홍삼엑기스의 산(pH) 및 온도처리에 의한 기능성 사포닌 함량증대)

  • In Jun-Gyo;Lee Bum-Soo;Kim Eun-Jeong;Park Myung-Han;Yang Deok-Chun
    • Korean Journal of Plant Resources
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    • v.19 no.1
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    • pp.139-143
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    • 2006
  • To increase the contents of functional ginsenosides by conversion, especially ginsenoside-$Rg_3$ and $Rh_2$, the extracts of red ginseng were treated with high temperature and citric acid or apricot extract. When the extracts were subject to $120^{\circ}C$ for 2 hours, the content of ginsenoside-$Rg_3$ was increased 2 times than in control. When the extracts were subject to $120^{\circ}C$ and acidic conditions adjusted with citric acid, the ginsenoside-$Rg_3$, was detected 2.8 times, but other ginsenoside were decreased heavily to 65%. When the extract were treated with for 12 hours at $80^{\circ}C$, the content of ginsenoside-$Rg_3$ was increased to 3.3 times, Also, when the red ginseng extracts were treated with apricot extract, the ginsenoside-$Rg_3$ was detected to 4 times than in control, but other ginsenoside were decreased lightly to 35%, not same as at the $120^{\circ}C$ treatment.

Bio-ethanol Production from Alkali Prehydrolyzed Yellow Poplar (Liriodendron tulipifera L.) Using Enzymatic Saccharification and Fermentation (알칼리 전처리 백합나무(Liriodendron tulipifera L.)의 효소당화 및 발효에 의한 바이오 에탄올 생산)

  • Shin, Soo-Jeong;Cho, Dae Haeng;Han, Sim-Hee;Kim, Young Hwan;Cho, Nam-Seok
    • Journal of Korean Society of Forest Science
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    • v.98 no.3
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    • pp.305-310
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    • 2009
  • Yellow poplar was selected a promising biomass resources for bio-ethanol production through alkali prehydrolysis, enzymatic saccharification and fermentation using commercial cellulase mixtures (Celluclast 1.5L and Novozym 342 mixtures) and fermenting yeast. In alkali prehydrolysis, 51.1% of Yellow poplar biomass remained as residues, which chemical compositions were 82.2% of cellulose, 17.6% of xylan and 2.0% of lignin. In alkali prehydrolysis process, 96.9% of cellulose, 38.0% of xylan and 5.7% of lignin were remained. Enzymatic saccharification by commercial cellulases led to 87.0% of cellulose to glucose and 87.2% of xylan to xylose conversion. Produced glucose and xylose were fermented with fermenting yeast (Saccharomycess cerevisiae), which resulted in selective fermentation of glucose only to bio-ethanol. Residual monosaccharides after fermentation were consisted to 0.4-1.4% of glucose and 92.1-99.5% of xylose. Ethanol concentration was highest for 24 h fermentation as 57.2 g/L, but gradually decreased to 56.2 g/L for 48 h fermentation and 54.3 g/L for 72 h fermentation, due to the ethanol consumption by fermenting yeast.

High-Level Production of Low-Branched Levan from Pseudomonas aurantiaca S-4380 for the Production of $di-\beta-D-Fructofuranose$ Dianhydride IV

  • JANG KI-HYO;JANG EUN-KYUNG;KIM SEUNG-HWAN;KIM IN-HWAN;KANG SOON AH;KOH ISSAC;PARK YOUNG-IL;KIM YOUNG-JUN;HA SANG-DO;KIM CHUL HO
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.102-108
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    • 2006
  • The IscA gene, encoding a levansucrase of 424 amino acids (aa) residues, was cloned from the genomic DNA of Pseudomonas aurantiaca S-4380, and overexpressed in Escherichia coli. The recombinant levansucrase overexpressed in E. coli was then used to produce levan from sucrose. Levan crystals with 98% purity could be obtained from the reaction mixture with $62\%$ yield using an alcohol precipitation method. The molecular weight of the levan was $7\times10^5$ daltons. Methylation studies showed that the levan was branched: main linkage C-2,6; branched linkage C-2,1; and degree of branching $6\%$. Three bacterial levans from different strains were incubated with levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032, which produced $di-\beta-D-fructofuranose$ dianhydride IV (DFA IV); final conversion yields from the levans to DFA IV were $39\%$ in Zymomonas mobilis, $53\%$ in Serratia levanicum, and $59\%$ in P. aurantiaca S-4380 levansucrase. The levan from P. aurantiaca S-4380 levansucrase gave the highest conversion yield of levan to DFAIV so far reported.

Analysis of Biomass Energy Potential and Density in Korea (국내(國內) 바이오매스 에너지 잠재량(潛在量) 및 밀집도(密集度) 분석(分析))

  • Kook, Jin Woo;Shin, Ji Hoon;Yoo, Ho Seong;Lee, See-Hoon
    • Resources Recycling
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    • v.22 no.5
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    • pp.56-62
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    • 2013
  • The biomass resources is one of promising ways to solve energy exhaustion issues and global warming issues at the same time. To evaluate domestic biomass resources potential such as agricultural wastes, forestry wastes, livestock wastes and municipal solid wastes, statistics data from various organizations were collected and analyzed in this study. Also, space energy densities of each districts in Korea were calculated and analyzed. The results from the evaluation of biomass energy potential and space energy densities in Korea might be useful to estimate the availability of biomass energy conversion processes and to choice a appropriate process to convert domestic biomass into energy.

Numerical Study on the Process Analysis of Biomass Fast Pyrolysis in a Circulating Fluidized Bed (순환유동층 반응기내 바이오매스의 급속열분해 공정해석에 관한 수치해석적 연구)

  • Lee, Yu Ri;Park, Hoon Chae;Choi, Myung Kyu;Choi, Hang Seok
    • Journal of Korea Society of Waste Management
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    • v.34 no.5
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    • pp.518-527
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    • 2017
  • The development of renewable energy is currently strongly required to address environmental problems such as global warming. In particular, biomass is highlighted due to its advantages. When using biomass as an energy source, the conversion process is essential. Fast pyrolysis, which is a thermochemical conversion method, is a known method of producing bio-oil. Therefore, various studies were conducted with fast pyrolysis. Most studies were conducted under a lab-scale process. Hence, scaling up is required for commercialization. However, it is difficult to find studies that address the process analysis, even though this is essential for developing a scaled-up plant. Hence, the present study carries out the process analysis of biomass pyrolysis. The fast pyrolysis system includes a biomass feeder, fast pyrolyzer, cyclone, condenser, and electrostatic precipitator (ESP). A two-stage, semi-global reaction mechanism was applied to simulate the fast pyrolysis reaction and a circulating fluidized bed reactor was selected as the fast pyrolyzer. All the equipment in the process was modeled based on heat and mass balance equations. In this study, process analysis was conducted with various reaction temperatures and residence times. The two-stage, semi-global reaction mechanism for circulating fluidized-bed reactor can be applied to simulate a scaled-up plant.

Characterization of Hyaluronic Acid Membrane Cross-linked with Lactide (락타이드로 가교시킨 히아루론산 막의 특성)

  • Kwon, Ji-Young;Cheong, Seong-Ihl
    • Polymer(Korea)
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    • v.29 no.6
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    • pp.599-604
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    • 2005
  • The hyaluronic acid (HA) with excellent biocompatibility has been combined with lactide, the ester dimer of polylactide, with good biodegradability to produce biocompatible materials which can control the period of degradation in a human body. By freeze frying method, HA and lactide were crosslinked with crosslinking agent, 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC). Degree of lactide and EDC reaction was determined by the analysis of nuclear magnetic resonance spectroscopy. Both lactyl group and EDC conversion increased as the mole ratio of lactide to HA increased from 5 to 13. The membrane swelled less and became more brittle with the more addition of lactyl group resulting from the higher mole ratio of lactide to HA. Swelling ratio decreased and tensile modulus increased due to the more addition of lactyl group as the EDC concentration increased or reaction temperature decreased. Drug release experiment from various membranes with different degree of crosslinking showed that permeability decreased with increasing degree of crosslinking. The degradation became slower with the more addition of lactyl group. Mechanical property and degradation rate of the synthesized membrane were shown to be controlled through adjusting operation parameters such as mole ratio, temperature, and crosslinking agent concentration.

Bioethanol Production from Popping Pretreated Switchgrass (팝핑전처리한 스위치그라스로부터 바이오에탄올 생산)

  • Kim, Hyun-Joo;Bae, Hyeun-Jong
    • Journal of the Korean Wood Science and Technology
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    • v.40 no.3
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    • pp.147-155
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    • 2012
  • Switchgrass was selected as a promising biomass resource for bioethanol production through popping pretreatment, enzymatic saccharification and fermentation using commercial cellulase and xylanase, and fermenting yeast. The reducing sugar yields of popping pretreated switchgrass after enzymatic saccharification were above 95% and the glucose in thesaccharificaiton solution to ethanol conversion rate after fermentation with $Saccharomyces$ $cerevisiae$ was reached to 89.6%. Chemical compositions after popping pretreatment developed in our laboratory were 40.8% glucose and 20.3% xylose, with much of glucose remaining and only xylose decreased to 4.75%. This means that the hemicelluloses area broke off during popping pretreatment. FE-SEMexamination of substrate particles after popping pretreatment was showed fiber separation, and tearing and presence of numerous micro pores. These changes help explain, enhanced enzymatic penetration resulting in improved hydrolysis of switchgrass particles after popping pretreatment.

Determination of Layer Thickness of A/B Type Multilayer Films in SIMS Depth Profiling Analysis

  • Hwang, Hyun-Hye;Jang, Jong-Shik;Kang, Hee-Jae;Kim, Kyung-Joong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.08a
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    • pp.231-231
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    • 2012
  • Correct determination of the interface locations is critical for the calibration of the depth scale and measurement of layer thickness in SIMS depth profiling analysis of multilayer films. However, the interface locations are difficult to determine due to the unwanted distortion from the real ones by the several effects due to sputtering with energetic ions. In this study, the layer thicknesses of Si/Ge and Si/Ti multilayer films were measured by SIMS depth profiling analysis using the oxygen and cesium primary ion beam. The interface locations in the multilayer films could be determined by two methods. The interfaces can be determined by the 50 at% definition where the atomic fractions of the constituent layer elements drop or rise to 50 at% at the interfaces. In this method, the raw depth profiles were converted to compositional depth profiles through the two-step conversion process using the alloy reference relative sensitivity factors (AR-RSF) determined by the alloy reference films with well-known compositions determined by Rutherford backscattering spectroscopy (RBS). The interface locations of the Si/Ge and Si/Ti multilayer films were also determined from the intensities of the interfacial composited ions (SiGe+, SiTi+). The determination of the interface locations from the composited ions was found to be difficult to apply due to the small intensity and the unclear variation at the interfaces.

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