• Title/Summary/Keyword: Bio Data

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Development of Sequence-Based DNA Markers for Evaluation of Phylogenetic Relationships in Korean Watermelon Varieties

  • Lee, Hee-Jeong;Cho, Hwa-Jin;Lee, Kyung-Ah;Lee, Min-Seon;Shin, Yoon-Seob;Harn, Chee-Hark;Yang, Seung-Gyun;Nahm, Seok-Hyeon
    • Journal of Crop Science and Biotechnology
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    • v.10 no.2
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    • pp.98-105
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    • 2007
  • Phylogenetic relationships in Korean watermelons were evaluated by genetic similarity coefficients using 15 SSR(simple sequence repeat), 14 SCAR(sequence characterized amplified region) and 14 CAPS(sequence characterized amplified region) markers. The SSR markers were selected from previously reported melon and watermelon SSRs through testing polymorphisms within a set of commercial $F_1$ varieties. The SCAR and CAPS markers were developed from polymorphic AFLP(amplified fragment length polymorphism) markers between inbred lines 'BN4001' and 'BN4002'. From the AFLP analysis, 105 polymorphic fragments were identified between the inbred lines using 1,440 primer combinations of EcoRI+CNNN and XbaI+ANNN. Based on the sequencing data of these polymorphic fragments, we synthesized sequence specific primer pairs and detected clear and reliable polymorphisms in 27 primer pairs by indels(insertion/deletion) or RFLP(restriction fragment length polymorphism). A total of 43 sequence-based PCR markers were obtained and polymorphic information content(PIC) was analyzed to measure the informativeness of each marker in watermelon varieties. The average PIC value of SCAR markers was 0.41, which was similar to that of SSR markers. Genetic diversity was also estimated by using these markers to assess the phylogenetic relationships among commercial varieties of watermelon. These markers differentiated 26 Korean watermelon varieties into two major phylogenetic groups, but this grouping was not significantly correlated with their morphological and physiological characteristics. The mean genetic similarity was 66% within the complete set of 26 commercial varieties. In addition, these sequence-based PCR markers were reliable and useful to identify cultivars and genotypes of watermelon.

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Consumer Perceptions and Consumer Behavior Toward Bio-Based Products: An Empirical Study in Vietnam

  • NGUYEN, Trong Luan;TRAN, Ngoc Phu;NGUYEN, Thi Kieu Thu;HUYNH, Thi Cam Tien;NGUYEN, Thi Kim Loi;THACH, Le Phuong Nghi;THAI, Gia Nhu;TRAN, Thi Thanh Sang
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.12
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    • pp.211-222
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    • 2021
  • Green economy, also known as sustainable economy, is a current development trend in which consumers prefer products that are wholly or partly derived from materials of biological origin since they have become more concerned about their health and the environment in which they live. This study aims to examine consumer behavior toward bio-based products with three key goals in mind. First, it helps to understand the perception of consumers toward Bio-based products. Second, it properly helps consumers be aware of products derived from materials of biological origin so that the consumer can make purchasing decisions to protect their health and contribute to the protection of the environment. Third, the study on consumer behavior towards bio-based products will provide a more accurate view and assessment to companies looking to develop Bio-based products. Based on that, the research is carried out through surveying, collecting data from consumers, and then using the deductive approach, descriptive statistics, and quantitative method analysis. The results demonstrated that a positive relationship and a direct impact are established between the variables of Attitude and Social Norms and the Purchase Intention toward Bio-based products. Furthermore, the findings reveal that customers have positive feelings towards bio-based products in terms of trust, knowledge, and the environment.

Simulation study on draft force prediction of moldboard plow according to cohesive soil particle size using the discrete element method (이산요소법을 활용한 점성토 환경에서의 토양 입자 크기에 따른 몰드보드 플라우 견인력 예측 시뮬레이션)

  • Min Seung Kim;Bo Min Bae;Dae Wi Jung;Jang Hyeon An;Se O Choi;Sang Hyeon Lee;Si Won Sung;Yeon Soo Kim;Yong Joo Kim
    • Journal of Drive and Control
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    • v.21 no.3
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    • pp.46-55
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    • 2024
  • In the agricultural machinery field, load analysis is mostly done through field tests. However, field tests are time-consuming and costly. There are also limitations in test conditions due to weather conditions. To overcome these environmental limitations, research on load analysis through simulation in a virtual environment is actively being conducted. This study aimed to select the most appropriate soil particle size for modeling by analyzing the effect of soil particle size on the prediction of draft force of the implement using simulation and soil particle modeling in a virtual environment with the discrete element method (DEM) software. The accuracy was verified by simulating the draft force for the same moving speed by soil particle size. For soil particle modeling, DEM soil modeling was performed by designing soil property measurement procedure. Soil particle correction was performed with a virtual vane shear test. Average DEM simulation results showed an error of 27.39% (19.43~40.66%) compared to actual measured data. The possibility of improvement was confirmed through additional research. Results of this study provide useful information for selecting soil particle size in soil modeling using DEM from the perspective of agricultural machinery research.

Correlations between Cell Abundance, Bio-volume and Chlorophyll $a$ Concentration of Phytoplankton Communities in Coastal Waters of Incheon, Tongyeong and Ulsan of Korea (식물플랑크톤 군집의 개체수, 생체량, chlorophyll $a$의 상관성; 인천, 통영, 울산 해역을 중심으로)

  • Joo, Hyoung-Min;Lee, Jin-Hwan;Jung, Seung-Won
    • Korean Journal of Environmental Biology
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    • v.29 no.4
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    • pp.312-320
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    • 2011
  • In order to estimate a better methodological factor to understand phytoplankton ecology between abundance and bio-volume of phytoplankton, each 1,160 phytoplankton data, including abundance, classification and chlorophyll $a$ concentration were collected in Korean coastal waters of Incheon (Yellow sea), Tongyeong (South sea), and Ulsan (East sea). Based on these data, phytoplankton bio-volume can be calculated through a geometric model. The correlation coefficient between abundance and chlorophyll $a$ concentration was higher than the coefficient between biovolume and chlorophyll $a$ concentration, because a small size phytoplankton has relatively dense chlorophyll contents compared with the proportion of chlorophyll in a large size phytoplankton. Thus, the interpretation using abundance to understand phytoplankton ecology in Korean coastal waters may be more effective than that using bio-volume.

H19 Gene Is Epigenetically Stable in Mouse Multipotent Germline Stem Cells

  • Oh, Shin Hye;Jung, Yoon Hee;Gupta, Mukesh Kumar;Uhm, Sang Jun;Lee, Hoon Taek
    • Molecules and Cells
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    • v.27 no.6
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    • pp.635-640
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    • 2009
  • Testis-derived germline stem (GS) cells can undergo reprogramming to acquire multipotency when cultured under appropriate culture conditions. These multipotent GS (mGS) cells have been known to differ from GS cells in their DNA methylation pattern. In this study, we examined the DNA methylation status of the H19 imprinting control region (ICR) in multipotent adult germline stem (maGS) cells to elucidate how epigenetic imprints are altered by culture conditions. DNA methylation was analyzed by bisulfite sequencing PCR of established maGS cells cultured in the presence of glial cell line-derived neurotrophic factor (GDNF) alone or both GDNF and leukemia inhibitory factor (LIF). The results showed that the H19 ICR in maGS cells of both groups was hypermethylated and had an androgenetic pattern similar to that of GS cells. In line with these data, the relative abundance of the Igf2 mRNA transcript was two-fold higher and that of H19 was three fold lower than in control embryonic stem cells. The androgenetic DNA methylation pattern of the H19 ICR was maintained even after 54 passages. Furthermore, differentiating maGS cells from retinoic acid-treated embryoid bodies maintained the androgenetic imprinting pattern of the H19 ICR. Taken together these data suggest that our maGS cells are epigenetically stable for the H19 gene during in vitro modifications. Further studies on the epigenetic regulation and chromatin structure of maGS cells are therefore necessary before their full potential can be utilized in regenerative medicine.

Estimation of TOC Concentration using BOD, COD in Runoff from Paddy Fields (영농기간동안 논 유출수 BOD, COD 자료를 이용한 TOC 농도 추정)

  • Choi, Dongho;Jung, Jaewoon;Yoon, Kwangsik;Lee, Kyoungsook;Choi, Woojung;Lim, Sangsun;Park, Hana;Yim, Byungjin;Hwang, Taehee
    • Journal of Korean Society on Water Environment
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    • v.28 no.6
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    • pp.813-818
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    • 2012
  • BOD and COD are currently used for water quality indices, but adoption of TOC for TMDL (Total Maximum Daily Load) is being suggested. Estimation of TOC using existing BOD and COD data is very important to diagnosis water quality trend when TOC is used for water quality index of organic matter in the future. The relationships between BOD COD and TOC in runoff from paddy fields were investigated during 2008-2011. The observed mean concentration of EMCs (Event Mean Concentration) for BOD, COD, TOC were 3.87, 10.97, 7.26 mg/L respectively. The correlation coefficients between BOD-TOC and COD-TOC were 0.42, 0.73, respectively. The coefficient of determination of regression equation for BOD-TOC and COD-TOC were 0.18, 0.53 respectively. Even though, conversion of COD data to TOC seems to be rather reliable than that of BOD-TOC, further monitoring is recommended to ensure better interpretation of relationship among BOD, COD and TOC.

Biometrics System Technology Trends Based on Biosignal (생체신호 기반 바이오인식 시스템 기술 동향)

  • Choi, Gyu-Ho;Moon, Hae-Min;Pan, Sung-Bum
    • Journal of Digital Convergence
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    • v.15 no.1
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    • pp.381-391
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    • 2017
  • Biometric technology is a technology for authenticating a user using the physical or behavioral features of the inherent characteristics of the individual. With the necessity and efficiency of the technology in the fields of finance, security, access control, medical welfare, inspection, and entertainment, the service range has been expanding. Biometrics using biometric information such as fingerprints and faces have been exposed to counterfeit and disguised threats and become a social problem. Recent studies using a bio-signal from the inside of the body other than the bio-information of the external body are being developed. This paper analyzes the recent research and technology of biometric systems using bio-signals, ECG, heart sounds, EEG, and EMG to present the skills needed for the development direction. In the future, utilizing the deep learning to build and analyze database to manage bio-signal based big data for the complex condition of individuals, biometrics technologies suitable for real time environment are expected to be researched.

Analysis of National R&D Patent Performance Network in Bio-Healthcare Sector (바이오 헬스케어 분야 국가연구개발 특허성과 네트워크 분석)

  • Kwon, Young-Eun;Kim, Jaesoo
    • Journal of the Korea Convergence Society
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    • v.9 no.12
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    • pp.17-24
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    • 2018
  • This study attempted to analyze technology convergence structure and key technology research sectors in bio-health. For this, network analysis was performed based on the patent outcomes achieved through national R&Ds. Then, a patent network was analyzed to derive problems and collect data from the National Science & Technology Information Service. With the five groups obtained through the analysis of IPC network and national R&D patents in bio-health based on a research frame network, topics were chosen based on the bio-healthcare technology system. Then, the technology with the greatest ripple effects was derived and compared to other sectors, suggesting a direction for national R&D investments. It is anticipated that this study would make a contribution to a search for R&D investment direction by additionally analyzing overseas patent data and improving correlation analysis between technology convergence and government-led R&D expenses.

Safety Evaluation of Individual Intersection Considering the Bio-Response (Electroencephalography) and the Cognitive Characteristics (생체반응(뇌파)과 인지평가 특성에 의한 개별 교차로 안전성 평가에 관한 연구)

  • Namgung, Moon;Lee, Byung Joo;Seo, Im Ki
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.3D
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    • pp.231-240
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    • 2010
  • As majority of the traffic accidents in intersections is caused by human factor, a close examination is required on its contributing factors through measuring the psychological and physiological response according to the driving characteristics of the drivers and the road conditions. In this study, for the safety evaluation of individual intersection considering human factors of the drivers, electroencephalography reaction was measured utilizing cutting-edge measuring equipment and survey on drivers' cognitive characteristics in ordinary times and while driving test was conducted. The relationship between the electroencephalography response when approaching the intersection and cognitive evaluation survey data in driving test was clarified, and individual intersection safety evaluation model was built considering cognitive evaluation factor and the reaction of a bio-response electroencephalography data. As a result, I could find out that cognitive evaluation was made through the reaction of a bio-response (Electroencephalography) process because electroencephalography reaction of a bio-response showed differently by the physical characteristics of the intersection and cognitive evaluation had a difference.

Chlorophyll Fluorescence and Growth Response of Three South Korea Native Fern Species under In-door Light Intensity

  • Kyungtae Park;Bo Kook Jang;Cheol Hee Lee;Sang Yeob Lee;Ju Sung Cho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.63-63
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    • 2020
  • This study investigated the growth of native ferns under indoor light intensities to identify the introduction possibility as in-door ornamental plants. Three evergreen perennial fern species used in this experiment were Coniogramme japonica (Thunb.) Diels, Woodwardia japonica (L. f.) Sm., and Cyclosorus acuminatus (Houtt.) Nakai ex H. Itô. The light intensities were adjusted to 10, 50, 100 and 200 PPFD (µmol·m-2·s-1) based on the measurement of the various indoor light quantities. The experiment was conducted for a total of 8 weeks, and the light period (12/12h), temperature (25±1℃), and humidity (55±3%) were maintained during the experiment. The control plant group was grown in glass greenhouse for the same period. As the result of the study, in door C. japonica showed better growth under light intensities of 100, 200 PPFD. However, withering of the plants were observed under all light intensities except the control, which causes an ornamental value decrease. This seems to be related to the increase of DIo/RC value in chlorophyll fluorescence parameters. In the W. japonica growth data, the plant height was not significantly different from the control but, the leaf number decreased more than a two-fold. Also, the formed leaves turned brown and showed a poor growth and SPAD value at 200 PPFD had decreased significantly. Growth data of C. acuminatus was not significantly different with the control at all light intensities however, withering was observed at 100 and 200 PPFD. In chlorophyll fluorescence parameters, significant decrease in Pi_Abs and increase in DIo/RC value at 200 PPFD impose that stress caused by the intense light might be the reason of the withering of the plants.

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