• Title/Summary/Keyword: Binary

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In vitro Propagation of Transgenic Ginsengs Introduced with Ferritin Light Heavy Chain Gene through Single Embryo Culture (Ferritin Light Heavy Chain 유전자가 도입된 인삼형질전환체의 단일배발생을 통한 식물체의 기내증식)

  • 윤영상;김종학;김무성;양덕춘
    • Korean Journal of Plant Resources
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    • v.17 no.2
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    • pp.161-168
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    • 2004
  • Optimal regeneration conditions of ginseng transformants were studied. It has been known that Ferritin Light Heavy Chain (FLHC) gene remove the several heavy metal by combination, store and transport. To obtain the ginseng tolerant to heavy metal, binary vector was introduced in Agrobacterium by tri-parental mating and then Agrobacterium tumefaciens MP90/FLHC was selected on the AB media and MS media containing kanamycin. Explants were co-cultured with Agrobacterium tumefaciens MP90/FLHC, which contained NPT II as a selectable marker, tadpole ferritin heavy chain (FLHC) gene and human ferritin light chain gene and then a number of embryos were induced. The induced embryo transferred to shooting media consisting of MS medium supplemented with GA 10 mg/L. As a result of examination that induced the normal growth of transfomants, transformants showed the equivalent growth in both root and shoot on the media containing the 1/3 MS.

Molecular Characterization of a Novel Vegetative Insecticidal Protein from Bacillus thuringiensis Effective Against Sap-Sucking Insect Pest

  • Sattar, Sampurna;Maiti, Mrinal K.
    • Journal of Microbiology and Biotechnology
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    • v.21 no.9
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    • pp.937-946
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    • 2011
  • Several isolates of Bacillus thuringiensis (Bt) were screened for the vegetative insecticidal protein (Vip) effective against sap-sucking insect pests. Screening results were based on $LC_{50}$ values against cotton aphid (Aphis gossypii), one of the dangerous pests of various crop plants including cotton. Among the isolates, the Bt#BREF24 showed promising results, and upon purification the aphidicidal protein was recognized as a binary toxin. One of the components of this binary toxin was identified by peptide sequencing to be a homolog of Vip2A that has been reported previously in other Bacillus spp. Vip2 belongs to the binary toxin group Vip1-Vip2, and is responsible for the enzymatic activity; and Vip1 is the translocation and receptor binding protein. The two genes encoding the corresponding proteins of the binary toxin, designated as vip2Ae and vip1Ae, were cloned from the Bt#BREF24, sequenced, and heterologously expressed in Escherichia coli. Aphid feeding assay with the recombinant proteins confirmed that these proteins are indeed the two components of the binary toxins, and the presence of both partners is essential for the activity. Aphid specificity of the binary toxin was further verified by ligand blotting experiment, which identified an ~50 kDa receptor in the brush border membrane vesicles of the cotton aphids only, but not in the lepidopteran insects. Our finding holds a promise of its use in future as a candidate gene for developing transgenic crop plants tolerant against sap-sucking insect pests.

The Study of Bit Split Methods and Double Binary Turbo Code for Additional Service of Advanced T-DMB (차세대 T-DMB 방송의 부가 서비스 제공을 위한 비트 분리 방법 및 Double Binary Turbo 부호 적용 연구)

  • Kim, Nam-Soo;Bae, Jong-Tae;Kim, Min-Hyuk;Jung, Ji-Won
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.10
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    • pp.1065-1074
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    • 2008
  • T-DMB(Terrestrial Digital Multimedia Broadcasting) system, is based on the Eureka-147 standard, provides various multimedia data services. However T-DMB system needs upgrades for more various services and better services. Therefore, we proposed advanced T-DMB system using unequal error protection system and hierarchical modulation in this paper while maintaing backward compatibility. And we simulated various bit split methods and double binary turbo code of DVB-RCS standard as method of LP data stream for additional service of advanced T-DMB. As the simulation results, double binary turbo code of proposed advanced T-DMB system have each coding gain of over 2 dB compared to conventional T-DMB.

Enhancement of HCB Tree for Improving Retrieval Performance and Dynamic Environments (검색 성능 향상과 동적 환경을 위한 HCB 트리의 개선)

  • Kim, Sung Wan
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.2
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    • pp.365-371
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    • 2015
  • CB tree represents the binary trie by a compact binary sequence. However, retrieval time grows fast since the more keys stored in the trie, longer the binary sequences are. In addition it is inefficient for frequent key insertion/deletion. HCB tree is a hierarchical CB tree consisting of small binary tries. However it can not avoid shift operations and have to scan an additional table to refer child or parent trie. In order to improve retrieval performance and avoid shift operations when keys are inserted or deleted, we in this paper represent each separated trie by a full binary trie and then assign the unique identifier to it. Finally the theoretical evaluations show that both the proposed approach and HCB tree provides better than CB tree for key retrieval. The proposed approach shows the highest performance in case of key insertion/deletion and moreover requires only 71%~89% of storage as compared with CB tree.

Measurement of distribution stability of binary nanofluids by zeta-potential (Zeta-potential을 이용한 이성분 나노유체의 분산안정도 측정)

  • Lee, Kang-Il;Jung, Chung-Woo;Kim, Hyun-Jun;Joeng, Jin-Hee;Kang, Yong-Tae
    • Proceedings of the SAREK Conference
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    • 2006.06a
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    • pp.52-57
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    • 2006
  • This study investigates the distribution stability of binary nanofluids where binary mixtures such as $NH_3/H_2O$ and $H_2O/LiBr$ solution are used as a base fluid. When a little amount of certain nanosized particles is added into a basefluid, the thermal conductivity of that mixture increases greatly. Such mixtures are named 'nanofluids' where nano-particles should be distributed stably and uniformly so the distribution stability of nanoparticles in nanofluids is one of the most important factors for nanofluid application. Therefore, binary nanofluids in which binary mixtures are applied as the basefluids are considered as working fluids. The kind and the concentration of nanoparticles, and the concentration of ammonia are considered as the key parameters. The objectives of this paper are to visualize the dispersed status of particles in binary nanofluids and to find the effect of key parameters on the distribution stability in the ammonia absorption system.

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Secure AKA(Authentication and Key Agreement) Protocol for Binary CDMA Network (Binary CDMA 망을 위한 안전한 AKA 프로토콜)

  • Kim, Yong-Hee;Park, Mi-Ae;Cho, Jin-Woong;Lee, Hyeon-Seok;Lee, Jang-Yeon;Yi, Ok-Yeon
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.20 no.1
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    • pp.51-61
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    • 2010
  • Koinonia system is designed to fully utilize the advantage of Binary CDMA so as to guarantee QoS in wireless networks. In this paper, we propose the new network structure based on this system and refer to it as BLAN(Binary CDMA LAN). Although BLAN is similar structure to IEEE 802.11 WLAN, it will ensure the fast handover and QoS. We also propose the AKA(Authentication and Key Agreement) protocol and Reauthentication protocol to be used for communication in BLAN. These protocols are securely and efficiently designed using the user identity module to support the more powerful authentication. Hence, BLAN, including the proposed protocols, will support the high mobility and security. In conclusion, we expect that BLAN can be applied to future infrastructure on special environment, and it can be helpful showing the new network model which alternate WLAN.

Design and Implementation of a Dynamic Instrumentation Framework based on Light-weight Dynamic Binary Translation (경량 동적 코드 변환 기법을 이용한 동적 인스트루멘테이션 기법 설계 및 구현)

  • Kim, Jeehong;Lee, Dongwoo;Kim, Inhyeok;Eom, Young Ik
    • Journal of KIISE
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    • v.41 no.11
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    • pp.892-899
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    • 2014
  • Dynamic binary instrumentation is a code insertion technique for debugging a program without scattering its execution flow, while the program is running. Most dynamic instrumentations are implemented using dynamic binary translation techniques. Existing studies translated program codes dynamically by parsing the machine code stream to intermediate representation (IR) and then applying compilation techniques for IRs. However, they have high overhead during translation, which is a major cause of difficulty in applying the dynamic binary translation technique to the program which requires high responsiveness. In this paper, we introduce a light-weight dynamic binary instrumentation framework based on a novel dynamic binary translation technique which has low overhead while translating the program code. In order to reduce the translation overhead, our approach adopts a tabular-based address translation and exploits a translation bypassing scheme, which stores the translated address of a frequently called library function in advance. It then accesses the translated address and executes function codes without code translation when calling the function. Our experiment results demonstrated that the proposed approach outperforms the prior dynamic binary translation techniques from 2% up to 65%.

Sintering and grain growth in binary forsterite(Mg$_2$SiO$_4$)/spinel (MgAl$_2$O$_4$) system

  • Kim, Sungjin;Han, Young-Hwan;Cho, Kyeong-Sik
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.11 no.2
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    • pp.78-84
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    • 2001
  • The binary forsterite($Mg_2SiO_4$)/spinel ($MgAl_2O_4$) system, a possible refractory for industrial applications, is investigated for their density and grain growth the same firing conditions as the each component material between $1400^{\circ}C$ and $1700^{\circ}C$ ($1650^{\circ}C$). The forsterite grain growth exponent is established to be equal to 5 for all compositions within this binary system. Generally; the spinel addition to forsterite inhibited the forsterite grain growth. The activation energies for the forsterite grain growth of the eight compositions(weight ratio of forsterite/spinel) within the binary system are determined to be: 952$\pm$79(95/5), 363$\pm$37(90/10), 219$\pm$21(80/20), 220$\pm$44(70/30), 112$\pm$16(50/50), 112$\pm$23(30/70), 198$\pm$26(10/90), and 121$\pm$12(5/95) KJ/mol. The more forsterite is contained within the binary system, the higher value the activation energy for forsterite grain growth. It is considered that the forsterite grain growth at the higher forsterite compositions are more inhibited by spinel than that of the lower forsterite compositions.

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Eigenvoice Adaptation of Classification Model for Binary Mask Estimation (Eigenvoice를 이용한 이진 마스크 분류 모델 적응 방법)

  • Kim, Gibak
    • Journal of Broadcast Engineering
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    • v.20 no.1
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    • pp.164-170
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    • 2015
  • This paper deals with the adaptation of classification model in the binary mask approach to suppress noise in the noisy environment. The binary mask estimation approach is known to improve speech intelligibility of noisy speech. However, the same type of noisy data for the test data should be included in the training data for building the classification model of binary mask estimation. The eigenvoice adaptation is applied to the noise-independent classification model and the adapted model is used as noise-dependent model. The results are reported in Hit rates and False alarm rates. The experimental results confirmed that the accuracy of classification is improved as the number of adaptation sentences increases.

Experimental investigation of effective atomic numbers for some binary alloys

  • Sharma, Renu;Sharma, J.K.;Kaur, Taranjot;Singh, Tejbir;Sharma, Jeewan;Singh, Parjit S.
    • Nuclear Engineering and Technology
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    • v.49 no.7
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    • pp.1571-1574
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    • 2017
  • In the present work, the gamma ray backscattering technique was used to determine the effective atomic numbers for certain binary alloys. With the help of a muffle furnace, the binary alloys were synthesized using the melt quenching technique with different compositions of $_{82}Pb$, $_{50}Sn$, and $_{30}Zn$. The intensity distribution of backscattered photons from radioactive isotope $^{22}Na$ (511 keV) was recorded with the help of GAMMARAD5 [$76mm{\times}76mm$ NaI(Tl) scintillator detector] and analyzed as a function of both atomic number and thickness of the target material. The effective atomic numbers for the same binary alloys were also computed theoretically using the atomic to electronic cross-section method with the help of the mass attenuation coefficient database of WinXCom (2001). Good agreement was observed between theoretical and experimental results for the effective atomic numbers of all the selected alloys.