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Ascophyllum and its symbionts. VI. Microscopic Characterization of the Ascophyllum nodosum (Phaeophyceae), Mycophycias ascophylli (Ascomycetes) Symbiotum

  • Deckert, R. J.;Garbary, D. J.
    • ALGAE
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    • v.20 no.3
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    • pp.225-232
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    • 2005
  • Optical microscopy of recently living and cleared material of the fucoid, Ascophyllum nodosum (L.) Le Jolis, revealed novel aspects of its interaction with the ascomycete Mycophycias ascophylli (Cotton) Kohlmeyer and Kohlmeyer (previously Mycosphaerella ascophylli Cotton). Most host cells are associated with hyphae by lateral attachment of cell walls. Hyphae form extensive networks throughout the host thallus and show considerable differentiation in the various host tissues. In the base of epidermal cells, hyphae form multicellular rings around each host cell to produce a continuous network. In medullary regions, long, relatively unbranched and longitudinally aligned hyphae occur, with radial branches extending into cortical regions. Scattered in the inner cortex of host tissue are numerous multicellular nodes of smaller, polygonal to irregular shaped cells with five or more radiating arms of hyphae. Individual hyphal cells show a variety of specializations including swellings and appressoria-like attachments to some host cells. These observations provide the morphological basis for the mutualistic symbiosis supported by recent experimental work. We conclude that this association is best described by the term “symbiotum.”

Application of HACCP System in the Pork Industry in Korea - Review -

  • Chung, M.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.2
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    • pp.253-260
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    • 1999
  • Occurrences of foodborne disease outbreaks are increasing in Korea. Among the outbreaks, Salmonella, Staphylococcus aureus, Vibrio parahaemolyticus are the most important organisms and meat and meat product the major sources of infection. Hazard Analysis Critical Control Point system is a process control system designed to identify and prevent microbial and other hazards in food production. It is considered to be the best process management system by the National Advisory Committee for Microbiological Criteria for Foods, the National Academy of Science, and the Codex Alimentarius Commission. The Korean Ministry of Health and Welfare established a legal basis for the implementation of the HACCP system in Article 32-2 (Hazard Analysis Critical Control Point) of the Food Sanitation Act in December 1995. The Ministry of Agriculture and Forestry has granted research funds for the development of this model system for application to traditional food products and processed fishery products as well as to raw meats. Implementation of the HACCP system is an important step and the Korean food industry and the Government are focused on ensuring food safety in Korea.

Synthesis of Machine Knowledge and Fuzzy Post-Adjustment to Design an Intelligent Stock Investment System

  • Lee, Kun-Chang;Kim, Won-Chul
    • Journal of the Korean Operations Research and Management Science Society
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    • v.17 no.2
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    • pp.145-162
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    • 1992
  • This paper proposes two design principles for expert systems to solve a stock market timing (SMART) problems : machine knowledge and fuzzy post-adjustment, Machine knowledge is derived from past SMART instances by using an inductive learning algorithm. A knowledge-based solution, which can be regarded as a prior SMART strategy, is then obtained on the basis of the machine knowledge. Fuzzy post-adjustment (FPA) refers to a Bayesian-like reasoning, allowing the prior SMART strategy to be revised by the fuzzy evaluation of environmental factors that might effect the SMART strategy. A prototype system, named K-SISS2 (Knowledge-based Stock Investment Support System 2), was implemented using the two design principles and tested for solving the SMART problem that is aimed at choosing the best time to buy or sell stocks. The prototype system worked very well in an actual stock investment situation, illustrating basic ideas and techniques underlying the suggested design principles.

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Compounds from Non-polar Fraction of the Feces of Trogopterus xanthipes (오령지 저극성 분획으로부터의 화합물 분리)

  • Kim, Yuna;Shim, Sang Hee
    • Korean Journal of Pharmacognosy
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    • v.46 no.1
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    • pp.31-37
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    • 2015
  • Thirteen compounds were isolated from n-hexane layer of the extracts of feces of Trogopterus xanthipes. Their chemical structures were elucidated as lupeol (1), lupenone (2), simiarenol (3), epitaraxerol (4), taraxerone (5), fatty acid esters of 11-oxo-${\beta}$-amyrin (6), 12-oleane-3,11-dione (7), $5{\beta}$-stigmastan-$3{\alpha}$-ol (8), $5{\beta}$-stigmastan-$3{\beta}$-ol (9), $5{\alpha}$-stigmastan-3-one (10), $5{\beta}$-stigmastan-3-one (11), $5{\beta}$-cholestan-$3{\alpha}$-ol (12), and 2-methoxyphenanthrene (13) on the basis of spectroscopic data. Even though all the isolated compounds are known, to the best of our knowledge, all the compounds (1-13) are reported from this species for the first time.

Secondary Metabolites Produced by Endophytic Fungus, Arthrinium phaeospermum (내생균 Arthrinium phaeospermum이 생산하는 이차대사산물)

  • Xia, Xuekui;Shim, Sang Hee
    • Korean Journal of Pharmacognosy
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    • v.47 no.3
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    • pp.217-221
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    • 2016
  • Endophytic fungi have yielded a variety of secondary metabolites so far. In the course of the project to find bioactive secondary metabolites from cultures of endophytic fungi, an isolate of Arthrinium phaeospermum (JS 0567) was selected for chemical investigation. A large scale culture of this strain in rice media was extracted with an organic solvent and the extract was subjected to a serious of chromatography, which led to six metabolites. Their chemical structures were elucidated as 2,3,6,8-tetrahydroxy-1-methylxanthone(1), 2,3,4,6,8-pentahydroxy-1-methylxanthone(2), 3,4,6,8-tetrahydroxy-1-methylxanthone(3), 3,6,8-trihydroxy-1-methylxanthone(4), 2,4,2',4',6'-pentahydroxy-6-methylbenzophenone(5), and 5,7-di hydroxy-3-methylphthalide(6) on the basis of spectroscopic data. To the best of our knowledge, this is the first study on the secondary metabolites from Arthrinium phaeospermum.

Chemical Constituents from Non-polar Fraction of the Fruiting Bodies of Hericium erinaceum (노루궁뎅이 버섯 자실체의 비극성 분획에서 분리된 성분들)

  • Li, Wei;Shim, Sang Hee;Kim, Young Ho
    • Korean Journal of Pharmacognosy
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    • v.48 no.4
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    • pp.280-284
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    • 2017
  • Lion's Mane Mushroom, Hericium erinaceum, is a traditional edible mushroom widely used in culinary applications. It has been also used as a medicine in East Asian countries due to its various biological activities. Chemical investigation of fruiting bodies of this mushroom afforded many aromatic compounds, which were mostly isolated from polar fraction of its extracts. Herein we tried to investigate non-polar compounds from the extracts of this mushroom. $CHCl_3$-soluble fraction of the extracts was subjected to chemical investigation, which resulted in isolation of four compounds. Their chemical structures were elucidated as ircicerebroside (1), cortenuamide A (2), 1-D-arabinitol-monolinoleate (3), and cinnamic acid (4) on the basis of spectroscopic data. To the best of our knowledge, this is the first report of compounds 1, 2, and 4 from Hericium erinaceum.

Prediction of High Temperature Plastic Deformation Variables on Al 6061 Alloy (Al 6061 합금의 고온 소성변형 조건의 예측)

  • 김성일;정태성;유연철;오수익
    • Transactions of Materials Processing
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    • v.8 no.6
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    • pp.576-582
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    • 1999
  • The high temperature behavior of Al 6061 alloy was characterized by the hot torsion test in the temperature ranges of 400∼550℃ and the strain rate ranges of 0.05∼5/sec. To decide optimum deformation condition, three types of deformation maps were individually made from the critical strain (εc). deformation resistance(σp) and deformation efficiency (η). The critical strain(εc) for dynamic recrystallization (DRX) which was decided from the inflection point of strain hardening rate(θ) - effective stress (σ) curve was about 0.65 times of peak strain (εp). The relationship among deformation resistance (peak stress, σp), strain rate (ε), and temperature (T) could be expressed by ε=2.9×1013[sinh(0.0256σp]7.3exp (-216,000/RT). The deformation efficiency (η)which was calculated on the basis of the dynamic materials model (DMM) showed high values at the condition of 500∼550℃, 5/sec for 100% strain. The results from three deformation maps were compared with microstructures. The best condition of plastic deformation could be determined as 500℃ and 5/sec.

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Vitellogenin mRNA Induction in Male Carp Treated with Bisphenol A (비스페놀A 처리 수컷 잉어에서 비텔로제닌 mRNA의 발현)

  • 박응로;최필선;이철우;류지성;남성숙;류홍일;전성환;나진균;박광식
    • Toxicological Research
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    • v.17 no.3
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    • pp.181-186
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    • 2001
  • The estrogenic potency of bisphenol A using reverse trancriptase-PCR response of liver vitellogenin mRNA in male carp was studied. For this, six combination of primers which were synthesized on the basis of cDNA consensus region of various species, were evaluated and one pair of primers was selected as the best to show 286 bp size-transcript. By using the selected primers, vitellogenin mRNA induction in carp treated with bisphenol A was measured and the chemical showed dose-and time-dependent Induction response. From this result, it was concluded that RT-PCR technique wing the selected primers in this study can be wed to monitor the estrogenic effects exerted In carp living in Korean freshwater.

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The Distribution Analysis of PM10 in Seoul Using Spatial Interpolation Methods (공간보간기법에 의한 서울시 미세먼지(PM10)의 분포 분석)

  • Cho, Hong-Lae;Jeong, Jong-Chul
    • Journal of Environmental Impact Assessment
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    • v.18 no.1
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    • pp.31-39
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    • 2009
  • A lot of data which are used in environment analysis of air pollution have characteristics that are distributed continuously in space. In this point, the collected data value such as precipitation, temperature, altitude, pollution density, PM10 have spatial aspect. When geostatistical data analysis are needed, acquisition of the value in every point is the best way, however, it is impossible because of the costs and time. Therefore, it is necessary to estimate the unknown values at unsampled locations based on observations. In this study, spatial interpolation method such as local trend surface model, IDW(inverse distance weighted), RBF(radial basis function), Kriging were applied to PM10 annual average concentration of Seoul in 2005 and the accuracy was evaluated. For evaluation of interpolation accuracy, range of estimated value, RMSE, average error were analyzed with observation data. The Kriging and RBF methods had the higher accuracy than others.

Simulation of Daily Reservoir Inflow using Objective Function Based on Storage Error (저수량 오차를 목적함수로 한 저수지 일 유입량 모의)

  • 노재경
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.42 no.4
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    • pp.76-86
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    • 2000
  • The objective function of reservoir storage error was suggested to simulate daily reservoir inflow. DAWAST model, UMAX, LMAX, FC,CP, CE were calibrated. Daily reservoir inflow was imulated with calibrated parameters and reservoir storage was simulated on a daily basis. The simulated results were compared with the monthly results by Gajiyama equation and ten-day results by Tank rainfall-runoff model through equal value lines and hydrographs . DAWAST model showed the best results compared with Gajiymama equation and Tank model. Especially, DAWAST model showed a good agreement in dry periods. NEW concept using objective function of storage error was believed to be satisfactory and to be applied in estimating reservoir inflow.

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