• Journal of Biomedical Engineering Research
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• v.13 no.2
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• pp.155-164
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• 1992

Attachment and growth of mouse flbroblast cells on block copolypeptides were studied in the pres once or absence of serum proteins. Cells are rapidly attached to she polymer surface within 30 min regardless of substrate in the presence of serum. The number of flbroblast cells attached on the poly mer surface coated by collagen was larger than thats on the bare surface. Attachment of cells Is as a whole suppressed to a low level by the addltion of sodium azide in the absence of serum. Thls suE gests that the active attachment of cells requires the biological metabolism taking place on polymer substrates. In the presence of serum protein, flbroblast cells are more rapidly grown on the bolck co polymer consisting of poly(T-benzyl L-glutamate) (PBLG) and polyoxypropylene(POP) than on other block copolymers. These results were in agreement wish the data obatlned by an Inverted ml croscope.

• Journal of the Korean Chemical Society
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• v.55 no.2
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• pp.230-234
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• 2011

The present study describes the synthesis of 4,5-dihydro-6-(4-methoxy-3-methylphenyl)-3(2H)-pyridazinone derivatives. The synthesis of the first target compound, 4,5-dihydro-6-(4-methoxy-3-methylphenyl)-3(2H)-pyridazinone (1), was achieved by Friedel-Crafts acylation of o-cresyl methyl ether with succinic anhydride and subsequent cyclization of the intermediary g-keto acid with hydrazine hydrate. Condensation of compound 1 with aromatic aldehydes in the presence of sodium ethoxide affords the corresponding 4-substituted benzyl pyridazinones (3a-d). The dihydropyridazinone 1 underwent dehydrogenation upon treatment with bromine/acetic acid mixture to give (4). Pyridazine (5) has been synthesized upon the reaction of pyridazinone (1) with 1,3-diphenyl-2-propen-1-one under the Michael addition reaction. N-dialkylaminomethyl derivatives 6a-b have been obtained from the reaction of pyridazinone 1 with formaldehyde and secondary amine, whereas reaction of 1 with formaldehyde gives N-hydroxymethyl derivative (7). This study also includes the synthesis of the 3-chloropyridazine derivative 8 in excellent yield by heating pyridazinone 3b in phosphorus oxychloride. The behaviour of the chloro derivative toward sodium azide, benzyl amine and anthranilic acid was also studied. The proposed structures of the products were confirmed by elemental analysis, spectral data and chemical evidence.