• Title/Summary/Keyword: Bench scale

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Reduction of Hexavalent Chromium by Leachate Microorganisms in a Continuous Suspended Growth Culture (연속배양 체제에서의 침출수 미생물에 의한 6가 크롬이온의 환원)

  • Kim, Hyoun-Young;Oh, Young-Sook;Kim, Yeong-Kwan;Choi, Sung-Chan
    • Korean Journal of Microbiology
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    • v.34 no.3
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    • pp.126-131
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    • 1998
  • Reduction of hexavalent chromium to its trivalent form by leachate microorganisms was studied in batch and bench-scale continuous stirred tank reactor. The inoculum was a culture of microorganisms in leachate and capable of providing up to 90% chromate reduction during 72 h batch assay with $20mg\;Cr(VI)\;L^{-1}$ in minimal media containing different levels of leachate (10 to 60%) and glucose (50 to 200 mM). Addition of glucose increased the efficiency of chromate reduction, but adverse effect was observed with increase of leachate probably due to the competitive inhibition between chromate and sulfate ions. The continuous culture experiment was conducted for 124 days using synthetic feed containing different levels of chromate (5 to $65mg\;L^{-1}$) at room temperature. With a hydraulic retention time of 36 h, chromate reduction efficiency was mostly 100% when Cr(VI) concentrations in the reactor were in the range of 5 to $50mg\;L^{-1}$ Specific rate of Cr(VI) removal was calculated as $3.492mg\;g^{-1}\;protein\;h^{-1}$ during the period of 101~124 days from the start-up which showed 81.2% of average reduction efficiency. The results indicate the potential application of using leachate microorganisms for detoxification of hexavalent chromium in various chromium-contaminated wastewater from landfill or tannery sites.

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Change of Chemical and Microbial Properties during Fermentation of Cotton Waste for Oyster Mushroom Cultivation (느타리 재배용 폐면 발효 중의 화학성 및 미생물 상의 변화)

  • Jhune, Chang-Sung;Jang, Kap-Yeul;Cho, Soo-Muk;Oh, Se-Jong;Park, Jung-Sik;Weon, Hang-Yeon
    • The Korean Journal of Mycology
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    • v.32 no.2
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    • pp.105-111
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    • 2004
  • The changes of microflora and chemical characteristics during fermentation process of cotton waste for oyster mushroom cultivation were investigated with 5 l bench-scale reactors placed in an incubator at different temperatures ($40,\;50\;and\;60^{\circ}C$). Cotton waste was wetted to 70% moisture, and air flow rates to the substrate were 50, 100 and 300 cc/min. In processing of composting, the mesophilic bacterial population decreased sharply but thermophilic bacterial population increased. In case of fungi, both mesophilic and thermophilic population decreased. The daily $CO_2$ evolution showed little difference in all treatments, while $NH_3$ dropped sharply after 3 days. The desirable composting temperature and air flow based on the mycelial growth of oyster mushroom were $50^{\circ}C$ and 100 cc/min, respectively.

Decline in Extractable Veterinary Antibiotics in Chicken Manure-Based Composts during Composting (계분을 원료로 한 퇴비의 퇴비화 과정 중 동물용 항생물질 농도저감)

  • Kwon, Soon-Ik;Jang, Yeon-A;Kim, Kye-Hoon;Kim, Min-Kyeong;Jung, Goo-Bok;Hong, Seung-Chang;Chae, Mi-Jin;So, Kyu-Ho;Kim, Kwon-Rae
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.4
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    • pp.628-634
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    • 2012
  • Release of veterinary antibiotics (VSs) to agricultural environment through application of animal manure and/or animal manure-based composts to soils is of concern. The current study was conducted to examine decline of VAs during composting the chicken manure. For this, antibiotics free chicken manure (20 kg) and sawdust (10 kg) were added to the bench-scale composting apparatus and then the mixed material was spiked simultaneously with three VAs (chlortetracycline, CTC; sulfamethazine, SMZ; tylosin, TYL) at two different levels (10 and $20mg\;kg^{-1}$). Then the decline of VAs was determined using Charm II system during 53 composting period. For comparison, composting only chicken manure was included at VAs concentration of $10mg\;kg^{-1}$. During composting, the concentration of all three different VAs declined below the prospective guideline values ($0.8mg\;kg^{-1}$ for CTC, $0.2mg\;kg^{-1}$ for SMZ, and $1.0mg\;kg^{-1}$ for TYL) except CTC at $20mg\;kg^{-1}$ spiking when the chicken manure was composted together with sawdust. Interestingly, CTC at $10mg\;kg^{-1}$ spiking appeared to be declined under the guideline value without sawdust while SMZ was resistant to be declined without sawdust. Unlike CTC and SMZ, TYL showed immediate decline right after spiking TYL to composting materials regardless the spiking concentration and existence of sawdust. Appropriate composting procedure of chicken manure was able to decline the residual VAs in the manure below the prospective guideline value and the importance of organic substances on this decline was perceived.

Biotechnology for the Mitigation of Methane Emission from Landfills (매립지의 메탄 배출 저감을 위한 생물공학기술)

  • Cho, Kyung-Suk;Ryu, Hee-Wook
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.293-305
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    • 2009
  • Methane, as a greenhouse gas, is some 21~25 times more detrimental to the environmental than carbon dioxide. Landfills generally constitute the most important anthropogenic source, and methane emission from landfill was estimated as 35~73 Tg per year. Biological approaches using biocover (open system) and biofilter (closed system) can be a promising solution for older and/or smaller landfills where the methane production is too low for energy recovery or flaring and installation of a gas extraction system is inefficient. Methanotrophic bacteria, utilizing methane as a sole carbon and energy source, are responsible for the aerobic degradation (oxidation) of methane in the biological systems. Many bench-scale studies have demonstrated a high oxidation capacity in diverse filter bed materials such as soil, compost, earthworm cast and etc. Compost had been most often employed in the biological systems, and the methane oxidation rates in compost biocovers/boifilters ranged from 50 to $700\;g-CH_4\;m^{-2}\;d^{-1}$. Some preliminary field trials have showed the suitability of biocovers/biofilters for practical application and their satisfactory performance in mitigation methane emissions. Since the reduction of landfill methane emissions has been linked to carbon credits and trading schemes, the verified quantification of mitigated emissions through biocovers/biofilters is very important. Therefore, the assessment of in situ biocovers/biofilters performance should be standardized, and the reliable quantification methods of methane reduction is necessary.

Characteristics of Gasification for a Refused Plastic Fuel (플라스틱 고형 연료의 가스화 특성)

  • Chun, Young Nam;Lim, Mun Sup;Jo, Dae Young
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.11
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    • pp.636-641
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    • 2015
  • Waste energy conversion to SRF (Solid Refuse Fuel) has the effects not alternative fossil fuel usage but also the reduction of greenhouse gas. But the direct burning of the SRF including a plastic waste generates air pollution problem like soot, dioxin, etc. so that an application of pyrolysis and gasification treatment should be needed. The purpose of this study is to supply a basic thermal data of the pyrolysis gasification characteristics in the plastic-rich SRF which are needed for developing the novel pyrolyser or gasifier. To do so, a bench-scale test rig was newly engineered, and then experiments were achieved for the production characteristics of gas, tar, and char. While SRF sample, gasification air ratio, holding time changed as 2 g, 0.691, 32 min respectively, the $H_2$ 1.36%, $CH_4$ 2.18%, CO 1.88%, $Cl_2$ 15.9 ppm, HCl 6.4 ppm were composed. Also light tar benzene $4.03g/m^3$, naphthalene $0.39g/m^3$, anthracene $0.11g/m^3$, pyrene $0.06g/m^3$, gravimetric tar $18g/m^3$, and char 0.29 g was formed.

Study on the Usefulness of the Classification in Ophthalmic Optics' Curriculum for Students and Optometrist - Focusing on Three-year Educational System in Gyeonggi Province - (안경광학과 재학생과 안경사의 안경광학과 교과과정 분야별 유용도 - 경기지역 3년제 교과과정을 중심으로 -)

  • Lee, Ok-Jin;Park, Hee-Kyoung
    • Journal of Korean Ophthalmic Optics Society
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    • v.18 no.4
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    • pp.413-428
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    • 2013
  • Purpose: The purpose of this study was to evaluate students and optometrist opinion of the usefulness of different subjects of optometry curriculum.This date will then be used as base-line data during a process of curriculum development Methods: 292 of ophthalmic optic's students and 123 of optometrists were surveyed in the usefulness of curriculum (five-point scale) followed by statistical analysis of the results. Results: Optometrists gave a higher average score for curriculum usefulness (3.89) than the students (3.71). The subject which showed the highest usefulness for the student was optometry (4.55) but clinical practice was the highest for the optometrist (4.48). There was significant differences (p<.05) on optics, ophthalmology, basic science, optometrist related subject and foreign language but optometry and contact lenses showed high usefulness in common without significant difference. The usefulness of subject for the student showed significant difference according to gender, grade, age, and desired working place and has demonstrated significant difference depend on gender, age, scholarship, total working experience as an optometrist, workplace, and position for optometrists as well. Conclusions: It is necessary to continue research for the development of standard and bench-marked curriculum for ophthalmic optics and also the curriculum, which reflecting the usefulness and needs obtained from ophthalmic optics' students and optometrists need to be developed.

Pyrolytic Gasification Characteristics of Waste Tires and Waste Synthetic Resins (폐타이어 및 폐합성수지류의 건류가스화 특성)

  • 노남선;김광호;신대현;김동찬
    • Resources Recycling
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    • v.9 no.1
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    • pp.27-35
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    • 2000
  • Characteristics of pyrolytic gasification were examined for the waste tire and 7 types of waste synthetic resin, using a bench scale experimental facility. the product gas temperature of waste tires was $150~300^{\circ}C$ and the temperature profile in the combustion zone of the lower reactor part tended to be clearly distinguished from that in the gasification zone of the upper part. However, in the case of waste synthetic resins, there were no clear distinction and temperature fluctuation was severe, depending on the reaction time. Product gas quantity, which depends on that of supplied (1st) air, was found to be 105~135% of the 1st air amount at the steady state. The concentration of noncombustible components in product gas was 80~90 vol.% and the high heating value of the product gas calculated from gas compositions was 1,500~3,000 kcal/N㎥ for waste tire, and 300~2,900 kcal/N㎥ for waste synthetic resins, respectively. Heating value of product gas and combustible gas concentration were increased in proportion to 1st air amount when 1st air amount is below $0.35N\textrm{m}^3$/min.

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Study on Removal Efficiency of Complex Wastewater from Agricultural and Industrial Plant for Advanced Treatments (고도처리를 위한 농공단지 복합폐수의 처리효율 특성에 관한 연구)

  • Seo, Tae Won;Kim, Moon Suk;Park, Young Dal;Cho, Wook Sang
    • Journal of the Korea Organic Resources Recycling Association
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    • v.20 no.2
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    • pp.53-65
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    • 2012
  • This study was focused on removal efficiency of complex (or mixed) wastewater from agricultural and industrial plant for advanced treatments by HBR-II process, that was well known to be suitable to the treatment of livestock wastewater. The main purpose of this study was intended to evaluate the applicable feasibility of the HBR-II for revamping the present activated sludge process to the advanced one. And also, the settling study including the batch typed experimental column tests was performed to evaluate the coagulation stability of organic colloidal particles in wastewater. The mid-scale plant of HBR-II process between pilot and laboratory was used for this study. As F/M ratio remains constant in the range of 0.20~0.25 $BOD_5/Kg{\cdot}MLSS/Day$, the efficiency of biological treatment increased. It has been shown that the results of biodegradation study were, for removal efficiency(%), $BOD_5$ 98.4%, $COD_{Mn}$ 92.9%, SS 97.5%, T-N 91.3%, T-P 82.3%, respectively, which were relatively higher than other processes. From this study, HBR-II process would be well applied to the biological treatment of agricultural and industrial complex wastewater.

Effects of Initial Concentration and Nutrients in Treatment of petroleum Hydrocarbon Contaminated Soils using a Slurry-Phase Bioreactor (슬러리상 생물반응기를 이용한 석유계탄화수소 오염토양의 처리에 있어서 초기농도 및 영양소의 영향)

  • 김수철;남궁완;박대원
    • Journal of Korea Soil Environment Society
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    • v.3 no.3
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    • pp.45-53
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    • 1998
  • The purpose of this study was to evaluate effects of initial concentration and nutrients in treatment of petroleum hydrocarbon contaminated soils. The reactor used in this study was slurry-phase bioreactor of in-vessel type. Performance results on treatment of diesel fuel contaminated soils and micorbial growth were generated at the bench-scale level. The fate of TPH(Total Petroleum Hydrocarbon) and the microbial growth were evaluated in combination with biodegradation rate. Effect of initial loading levels of 50,000 and 100,000mg TPH/kg soil was studied. Performance results with two reactors were showed at the total TPH removal rate of 90.5% and 90.8%, respectively. However, the reactor with the initial concentration of 50,000mg TPH/kg soil showed higher biological TPH removal efficiency except for removal by volatilization than the other Although the different amount of nutrients was applied in two reactors, there was no remarkable difference in microbial growth rate. However, considerable factor in this results was that applied different initial concentration to two reactors. Although initial concentration was two times higher than it applied to the reactor without addition of nutrients, in total and biological TPH removal rate the reactor with addition of nutrients showed a higher than the other.

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Thermophilic Biohydrogen Production from Glucose with a Long-term Operation of CSTR (CSTR의 장기운전을 통한 포도당으로부터의 고온 수소생산)

  • Ahn, Yeong-Hee;Oh, You-Kwan;Park, Sung-Hoon
    • KSBB Journal
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    • v.20 no.6
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    • pp.425-430
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    • 2005
  • Thermophilic $H_2$ was produced for 1 year using a bench-scale continuous stirred tank reactor(CSTR). The CSTR was inoculated with anaerobically digested sludge after heat treatment and fed with a glucose-based medium. The reactor showed relatively short start-up period(30 days) and high maximal $H_2$ yield(2.4 mol $H_2/mol$ glucose). Keeping pH 5.0 or less suppressed methanogenic activity. Bacteria affiliated with Thermoanaerobacterium thermosaccharolyticum kept being dominant from approximately 40 days as determined by DGGE. Environmental perturbation(pH or temperature) caused the decrease of biomass concentration in the reactor and the instability of reactor performance, $H_2$ production rate and $H_2$ yield. The unstable performance was accompanied with high concentration of lactate in the effluent. Taken together, the poor recovery of CSTR after perturbations could be partly explained by low biomass concentration and/or metabolic shift of the major population in the CSTR.