• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.909-917
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• 2016

The shiitake mushroom industry has suffered from Camptomyia (gall midges) pest, which feeds on the mycelium of shiitake mushroom during its cultivation. It has been postulated that fungal damage of shiitake bed-logs is associated with infestation by the insect pest, but this is not well understood. To understand the fungal damage associated with Camptomyia pest, various Trichoderma species were isolated, identified, and characterized. In addition to two previously known Trichoderma species, T. citrinoviride and T. deliquescens, two other Trichoderma species, T. harzianum and T. atroviride, were newly identified from the pestinfested bed-log samples obtained at three mushroom farms in Cheonan, Korea. Among these four species, T. harzianum was the most evident. The results of a chromogenic media-based assay for extracellular enzymes showed that these four species have the ability to produce amylase, carboxyl-methyl cellulase, avicelase, pectinase, and ß-glucosidase, thus indicating that they can degrade wood components. A dual culture assay on PDA indicated that T. harzianum, T. atroviride, and T. citrinoviride were antagonistic against the mycelial growth of a shiitake strain (Lentinula edodes). Inoculation tests on shiitake bed-logs revealed that all four species were able to damage the wood of bed-logs. Our results provide evidence that the four green mold species are the causal agents involved in fungal damage of shiitake bed-logs infested by Camptomyia pest.

• The Korean Journal of Mycology
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• v.46 no.4
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• pp.495-503
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• 2018

Oak mushroom is cultivated using logs and sawdust media as substrates. In this study, fungi were isolated during a monitoring of indoor air in the oak mushroom cultivation houses located in Cheongyang-gun of Chungnam, Geoje-gun of Gyeongnam, Gumi-si of Gyeongbuk, Jangheung-gun of Jeonnam and Yeoju-si of Gyeongggi-do. Identification of the fungi based on morphology and molecular analysis of the internal transcribed spacer region and 28S rDNA, translation elongation factor translation elongation factor 1 a gene, and ${\beta}-tubulin$ gene revealed that six fungi, Cenangium acuum, Neopestalotiopsis surinamensis, Metarhizium marquandii, Periconia macrospinosa, Trichoderma petersenii, and Trichoderma paratroviride that have not been recorded previously in Korea.

• The Korean Journal of Mycology
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• v.41 no.1
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• pp.28-32
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• 2013

New Shiitake (Lentinula edodes) strain "Chunbaegko" was bred by Di-mon method. Bed-log cultivation of "Chunbaegko" was performed. Fruit-body production of "Chunbaegko" was most at spring and autumn. Optimal temperature of fruit-body formation was $14{\sim}22^{\circ}C$ Baegwhako, the best quality fruit-body, is produced during spring. The diameter of pileus is ca. 52 mm. The total amount of fruit-body production during 4 years(one generation) was 140 $kg/m^3$ log.

• Journal of Mushroom
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• v.11 no.1
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• pp.9-14
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• 2013

New shiitake (Lentinula edodes) strain "soohyangko" was bred for bed-log cultivation. Fruit-body production of "soohyangko" was most at summer and autumn. The fruit-body is hemispherical shape, brown colored and diameter of pileus is ca. 56 mm. Optimum temperature of fruit-body formation was $18{\sim}28^{\circ}$, and the fruiting is concentrated. The total amount of fruit-body production during 4 years (one generation) was $140kg/m^3$ log.

• Korean Journal of Agricultural Science
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• v.48 no.3
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• pp.483-492
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• 2021

This study attempted to estimate the degree of management of oak mushroom farms in Chungcheongnam-do and to provide information to establish oak mushroom cultivation-related policies. The oak mushroom management standard diagnostic table consists of three major categories, growing condition, inoculation management, cultivation management and management administration, along with 20 subcategories. Thus, 209 households of oak mushroom farms were surveyed from 2015 to 2018 in Gongju, Cheongyang, Buyeo and Seochun in Chungcheongnam-do. The average score for the 20 subcategories was 71.5 points (representing a significant level), indicating that these areas have excellent management conditions. The analysis of the management performance indicators revealed a high number of indicators with scores of five or above. The total score was higher, and the amount per bed log and the rate of top-grade products in the total output were also higher, indicating a significant correlation. These findings will provide consulting services to oak mushroom growers as they highlight the correlation between the higher scores of indicators in the oak mushroom management standard diagnostic table and the management performance of farmers. We found that the scores of the indicators for management administration, such as management record and analysis and fund plan were relatively lower than those of other indicators. It is assumed that the owners aging has led to negligence in recording the details on incomes, expenditures, and work and lowered the willingness to make substantial profits. Therefore, it is essential to overcome these problems for profitable oak mushroom farming.

• Journal of Practical Agriculture & Fisheries Research
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• v.10 no.1
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• pp.153-167
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• 2008

High temperature and natural sun light are considered as the core conditions for high quality and large quantity of Phellinus baumii production. However still now on there has been a mistake of excessively cutting off the natural light by spreading the closing nets on the mushroom cultivating house. For an example there are many houses where the closing nets under the roofs be extended to cover the sides of the houses, which way prevents the mushrooms in the houses from receiving sufficient natural sun light and getting the temperature sufficiently to grow so that the quantity and quality of the produced mushrooms are lowered even though the mushrooms can grow in those conditions. In order to avoid this mistake, the closing nets must be placed on the roofs of the houses only without dropping them to cover the sides. Further more when the closing nets are placed triply at the beginning stage of Phellinus baumii's growth in the house, the nets restrain the internal temperature of the house going up and intercept the natural bright light flowing into the house so that the growing tardiness occur to the Phellinus baumii. Therefore the roof only must have been covered by the closing net for 65% cutting off the light until May, and then covered by double folded the net for June, triple folded the net for July and August, double folded the net for September, and the single net for October. When the ventilation in the house has been maintained until the house tightly balloon out through controling lifting force of internal air, the Phellinus baumii can grow well while the bed logs themselves aren't dried out. Marketing is also very much important as well as increasing quality and quantity of Phellinus baumii production.

• Journal of Practical Agriculture & Fisheries Research
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• v.9 no.1
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• pp.33-46
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• 2007

We conducted this research to develop the medium for some edible mushroom cultivation using shiitake waste log which is abandoned after cultivation of shiitake mushroom because those bed logs can not be recycled. The isolates of P. ostreatus(POS-012), P. eryngii(PER-005), G. frondosa(GFR-001) and F. velutipes(FVE-001) were selected and examined for mycelial growth on sawdust media prepared from shiitake waste log. Mycelial growth of selected isolate were satisfactory on the sawdust extract media using acasia(Robinia pseudo-acacia), neutinamu(Zelkova serrata) and kangchamnamu(sangsuri, Quercus acutissima) which are no shiitake-inoculated. Although the mycelial growth of the isolate were poor on the sawdust media prepared from Quercus spp., sawdust of neutinamu, (Zelkova serrata), beotnamu, (Prunus serrulata), orinamu(Alnus japonica), eunsuweonsasinamu(Populus tomentiglandulosa) and chestnut(Castanea crenata) were excellent for mycelial growth. However, shiitake logs which are infected with harmful fungi such as Hypocrea spp. were useful as recycle materials for mushroom cultivation.

• Korean Journal of Plant Resources
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• v.18 no.3
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• pp.512-517
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• 2005

To establish the economical cultivation method of Gastrodia elata Blume by controlling the shading rate and the mixing ratio of recycling spawn bed logs, a serial experiment was carried out. When G. elata was grown in the field with no shading, the yield of spawn tuber was higher than that of tuber of G. elata. The yield of G. elata was high in the rainproof green house covered with one layer of $95\%$ shading net, in the rainproof green house covered with two layers of $95\%$ shading net, and in the field with no shading, in that order. In the case of mixing ratio of recycling spawn bed logs, the yield was high in the fresh logs, in the mixing ratio of 2:1, and the ratio of 1:1, in that order. The income of the rainproof green house covered with one layer of $ 95\%$ shading net which brought the best yield was $81\%$ in the mixing ratio of 2:1 over the fresh logs.

• Journal of Korean Society of Forest Science
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• v.80 no.4
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• pp.436-444
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• 1991

To increase both the production of mushrooms and the usages of the residues (used media) for roughage, the pinchips(PC) and sawdusts(SD) of three Korean oak species were tested as cultivating media for Lentinus edodes with varing the composition of the media. The results are as follows. 1. Organic acids(tannic acid and citric acid), which were added in the PC medium to enrich the quality of mushrooms, did not increase the growths of the mycelia and the quality. 2. The mushrooms were able to be harvested from six months after beginning the cultivation. No significant differences were found among the qualities of the mushrooms produced by various treatments. 3. The contents of crude proteins(1.82-4.55%) in the used media were higher than those in woods (c.a. 0.7%), and the total digestible nutrients (44.0-46.0%) in the used medium were not much different from those in rice straws (c.a.48.0%). 4. The contents of some essential amino acids (methionine in the used medium of the tannic acid added PC or S.D. and isoleucine, phenylalanine and lysine in the used medium of the untreated PC or SD) were higher than those in the control (raw media). The contents of a few other essential amino acids (threonine, valine and arginine) were lower in the used media. Most nonessential amino acids in the used media, particularly in the used bed-log, appeared to decrease than those in the control.

• Journal of Mushroom
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• v.11 no.4
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• pp.230-239
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• 2013

The major species of fungus gnats which caused the severe damage in shiitake farm were identified as a Bradysia difformis, B. alpicola, and Camtomyia cortocalis on oak log beds cultivation. The B. difformis occurred early in the middle of March while B. alpicola and C. cortocalis appeared since the beginning of May. The occurrence rate for adults of B. difformis showed highly at the end of July (11.9~1,774.2 in dong-myeon and 0.4~2,583.3 in pungse-myeon) in 2012 and mid-June (10.7~4,650 in dong-myeon and 36.8~4740 in pungse-myeon) in 2013. The counting numbers on the traps for B. alpicola reached highest peak in the middle of June (2.1~63.2 in dong-myeon and 1.0~21.7 in pungse-myeon) and the end of May (0.8~163.7 in dong-myeon and 0.5~280.5 in pungse-myeon) in 2012 and 2013, respectively. The number of C. cortocalis showed high record in the middle of May in 2012 (0.6~4.7) and in the middle of June (2.1~17.3) in 2013 in dong-myeon whereas showed the peaks in the middle of May (0.6~4.7) in 2012 and in the late of May (1.3~17.6) in 2013 in pungse-myeon. The fruiting bodies of shiitake mushroom by fungus gnats were severely damaged from mid-June to late-July and the damage rate were 0.625.5% (2012) and 0.7~30.5% (2013) in dong-myeon and 1.5%~21.6% (2012) and 1.9~36.8%(2012) in pungse-myeon. To investigate the control effect for fungus gnats by Hypoaspis aculeifer, H. aculeifer (30 mixutre of nymph and adult per $m^2$) were treated to oak log beds shiitake cultivation for six times (May 2 and 28, June 25, July 10 and 25 and August 28). The occurrence rate of adults and damage rate of fruiting bodies of 3 major species reduced 79.3% (adult numbers) and 74.8% (fruiting bodies) in dong-myeon and 64.1% (adult numbers) and 65.5% (fruiting bodies) in pungse-myeon, respectively, compared to non-treatment. Accordingly, H. aculeifer effectively controlled the fungus gnats on shiitake mushroom and can be used as good control agent.