• 한국잠사곤충학회지
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• 제37권2호
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• pp.167-171
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• 1995

딱정벌레목 천공성 해충인 뽕나무 하늘소(Apriona germari)의 효과적인 방제를 위하여, 뽕나무 하늘소 이병충으로 부터 곤충병원 사상균을 분리하고, 위상차 현미경 및 주사전자현미경으로 관찰하였으며, PCR(polymerase chain reaction)을 이용하여 동정하였다. 뽕나무 하늘소 이병충으로 부터 분리된 곤충병원 사상균은 현미경 관찰 결과 Beauveria속의 전형적인 형태적 특성을 나타냈다. 따라서 이들의 용이한 동정을 위하여 PCR primer(5'-ACG GGC GCT C-3')를 이용한 RAPD(random amplification of polymorphic DNA) 방법으로 분석하고, B. bassiana와 B. brongniartii의 PCR 산물을 전기영동한 결과 DNA 표식자로 이용이 가능하였다. 이상의 결과로서 본 실험에서 분리·명명된 SFB-1A는 B. bassianifh, SFB-1A는 B. bassiana로, SFB-3A는 B. brongniartii로 동정되었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• 한국잠사곤충학회지
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• 제38권1호
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• pp.31-35
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• 1996

흰굳음병균(B. bassiana)을 이용하여 효과적인 진균살충제 개발을 위해, 진균의 대량배양 체계에서 가장 중요한 고체배지를 저가의 농가부산물인 밀기울을 이용하여 조제하고, 고체배지의 물성, 수분, 온도 조건, 고체배지의 형태 및 크기에 따른 B. bassiana 포자의 성장을 조사하였다. 그 결과, 고체배지는 powder 형보다 pellet 형이 포자의 성장에 효과적이었으며, 고형제가 첨가된 P-II 배지보다는 첨가되지 않은 P-I 배지가 효율적이었다. 또 수분 요구량은 배지 : 물 (W/V)의 비율이 4:2 조건 이상, 온도조건은 2$0^{\circ}C$와 $25^{\circ}C$에서 높은 성장율을 보여주었다. 그리고 고체 배지의 형태에 따른 포자의 생산성은 S형 P-I 고체배지 (직경 3mm)에서 효과적이었다. 이상의 결과, S형 P-I 고체배지는 흰굳음병균 포자의 대량배양에 경제적이고, 효율적으로 이용될 수 있을 것이다.

• Mycobiology
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• 제34권1호
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• pp.1-6
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• 2006

A Cordyceps species was found with a Beauveria anamorph state on larval insect cadavers on Obong mountains in Gangwon Provinces, Republic of Korea. Cultures from discharged ascospores formed an anamorph identifiable as Beauveria bassiana. This teleomorph-anamorph connection was also confirmed by the in vitro production of fertile ascomata from conidial cultures with morphology like that of field-collected specimen. This is the first report of in vitro production of a teleomorph for any Beauveria species. The Cordyceps species has been conspecified as Cordyceps bassiana, a species described from China with B. bassiana anamorph.

• 한국응용곤충학회지
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• 제36권1호
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• pp.37-42
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• 1997

우리나라의 곤충병원성곰팡이 분포를 조사하기 위하여 5개도와 3개 광역시에서 200개소의 토양 sample을 채집하여 꿀벌부채명나방 유충을 이용하여 분리하였다. 8종의 곤충 사체로 부터는 직접 분리하였다. 그 결과 40개소(20.0%)의 토양과 8종의 곤충 사체에서 곰팡이가 분리되었는데, 토양에서 분리된 균종별로는 Bequveria가 21개소(10.5%), Metarhizium이 19개소(9.5%)이었다. 그외 7종의 곤충에서 Beauvria가 분리되었다. 서식처별로는 산림 110개소중 25개소(22.7%), 경작지 20개소중 2개소(11.0%), 휴한지 11개소중 6개소(54.5%), 하천 제방 19개소중 7개소(36.8%)에서 곤충병원성곰팡이가 검출되었다. 한편, 곤충 사체에서는 긴수염 대벌레에서 Metarhizium이 분리된 것을 제외하고는 모두 Beauveria가 분리되었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제14권2호
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• pp.81-85
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• 2007

White Muscardine is the most common fungal disease of silkworm, Bombyx mori L. caused by the pathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin. In the present investigation, an attempt has been made to screen locally available medicinal/ weed plants against Beauveria bassiana. Among the plant extracts (PE) tested, 5% aqueous crude extract of the bulb of Allium sativum (Garlic) has been found to be most effective against Beauveria bassiana. The radial growth of Beauveria bassiana in vitro was inhibited to the tune of 54.9% in aqueous extract and 54.4% in ethanolic extract of Allium sativum and correspondingly mycelial dry weight gave rise to 110.7 mg and 108.7 mg against 201.7 mg in control 15 days post treatment. Similarly, silkworm larvae topically inoculated with the Beauveria bassiana conidia ($1.8{\times}10^6/ml$) registered survival up to 53.0% against 0.0% in control after treatment with aqueous extract of Allium sativum. Simultaneously, as a preventive measure, silkworm larvae were put to rear in conidia contaminated seat paper instantly treated with aqueous extract of Allium sativum that also increased survival up to 61.0% against 4.6% in control. It is also observed that the plant extract is absolutely innocuous to silkworm.

• 한국미생물·생명공학회지
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• 제27권5호
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• pp.415-418
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• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• 한국식물병리학회지
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• 제12권2호
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• pp.245-250
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• 1996

식물병원균 Botrytis cinerea와 Fusarium oxysporum에 대한 생물학적 방제의 기초자료를 얻기 위하여 살충성 진균 Beauveria bassiana의 식물병원균에 대한 균사성장저해, 포자발아 억제, 균사와 포자의 형태변화 등의 효과를 살펴보았다. 평판배지 상에서 두 식물병원균의 균사생장이 저해되었으며, 저해효과는 배지종류에 따라 달랐는데 B. cinerea의 경우 PDA배지에서 가장 크게 저해되었으며 F. oxysporum의 경우에는 TSA배지에서 가장 크게 저해되었다. B. bassiana는 $25^{\circ}C$에서 6일간 배양했을 때 F. oxysporum에 대한 항균력이 가장 높았으며 동시에 최대의 균체량을 생산하였다. 또한 B. bassiana의 배양여액을 식물병원균에 30% 농도로 첨가하여 배양했을 때 식물병원균의 포자 발아율은 B. cinerea, F. oxysporum에서 각각 30%(control: 88.2%), 10.0%(control: 78.6%)로 낮아졌으며 발아 개시 시간도 4~8시간 지연되었다. 현미경을 통한 미세구조관찰에서는 10%의 B. bassiana배양여액을 첨가했을 때 F. oxysporum의 포자 크기가 1/2~1/3으로 줄어들었으며 균사와 격막의 형태도 비정상적으로 변하여 균사외막과 격막-격막 사이의 구분이 불명확해졌다.

• KSBB Journal
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• 제14권3호
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• pp.365-370
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• 1999

To optimize the culture conditions for Beauveria bassiana 726, the effects of culture medium, pH, and temperature on mycelium and spore production were investigated. The optimum temperature and pH for the cultivation of B. bassiana 726 were 28 $^{\circ}C$ and 5.0, respectively. The optimized medium was composed of 1.0~2.0% total sugar from molasses, 0.5% corn steep liquor and 0.05% KH$_2$PO$_4$. In the cultivation of B. bassiana 726 with the optimum medium, the specific growth rate and substrate utilization were well-fitted with the proposed kinetic model in the shake flask and stirred tank reactor. When the fed-batch cultivation using carbon suorce, nitrogen source, and mineral salt as a feeding medium was compared with batch cultivation in stirred tank reactor, mycelium (12.7 g/L) and spore production (5.4$\times$$10^8/mL$) were enhanced up to 110% and 85%, respectively.

• Entomological Research
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• 제48권5호
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• pp.339-347
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• 2018

Seven different strains of Beauveria bassiana were used in a bioassay on Pieris rapae larvae. The results showed that an B. bassiana strain showed relatively high pathogenicity towards P. rapae larvae. The adjusted mortality rate was 92.86 %, and the infection rate was 85.71 % in 10 days post inoculation. Molecular identification was performed to identify the unknown strain. Internal Transcribed Spacer sequence analysis showed that the polymerase chain reaction amplicon length of the unknown strain of Beauveria sp. was 573 bp, and sequence similarity to the known B. bassiana sequences in the NCBI database was 99 %. The B. bassiana strain was named Bb01. The changes of proteins and PPO of P. rapae larvae infected by B. bassiana Bb01 strain at different times was determined. The activity of PPO increased in 1-6 d and decreased in 7 d again after inoculation. The B. bassiana invaded into the insect body affected the balance of the proteins and PPO.