• The Korean Journal of Malacology
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• v.15 no.1
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• pp.49-55
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• 1999

Mass mortality of the horned turban shell, Batillus cornutus was investigated using histological and bacteriological methods. Some pathogenic bacteria were isolated from mortal or inactive individuals. The pathogenic agents causing mortality of the horned turban shells were as Vibrio alginolyticus and V. anguillarum. Laboratory experiment indicated that optimal growth temperature of two bacteria was 25 to 30$^{\circ}C$ and 3% of NaCl. Histological examination of the horned turban shells showed that gill necrosis is one of the major symptom of infected individuals. It was believed that sudden increase of those two bacterial agents due to environmental change cause mortality of horned turban shells.

• The Korean Journal of Malacology
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• v.27 no.1
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• pp.69-76
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• 2011

Gill morphology and ultrastructure of the spiny top shell, Batillus cornutus were described using light and electron microscopy (SEM and TEM). The spiny top shell, Batillus cornutus has bipectinate gill. The epithelial layer of gill filament was simple and composed of columnar epithelium, ciliated cell, mitochondria-rich cell and secretory cell. Microvilli were well-developed on the free surface of columnar epithelial cell. The epithelial cells are connected to the neighboring cells with intercelluar junctions at the apico-lateral surface. The cilia and microvilli were commonly observed on the free surface of ciliated cell. Tubular mitochondria appeared in the apical cytoplasm, and connected ciliary rootlet. Mitochondria-rich cells contained a oval-shaped nucleus in the basal area. And majority of cytoplasm was occupied by well-developed mitochondria. Result of AB-PAS (pH 2.5) and AF-AB reaction showed that secretory cells contained mainly acidic carboxylated mucosubstances. Secretory cells are unicellular glands and can be divided into four types (A, B, C and D) depending on the cell shape and ultrastructure of secretory granules.

• The Korean Journal of Malacology
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• v.23 no.2
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• pp.227-233
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• 2007

The amount of Batillus cornutus captured in Jeju Island was about 2,000 tons/year for three years after 2000. The mean size of B. cornutus by shell height was 7.7 cm in 2001, 7.9 cm in 2002, and 8.1 cm in 2003. Local mean size of B. cornutus by shell height was 8.7 cm in eastern waters, 7.4 cm in western waters, 7.8 cm in southern waters, and 7.7 cm in northern waters of Jeju Island. To investigate the effect of the growth pattern, an experiment was conducted: the samples were tagged and released in southern coastal waters of Jeju Island on 2nd April (a release test) and 29th October (a recapture test) in 2003. The release stations were two sites, natural reef and artificial reef, where their environmental conditions were different from each other. In April, the size of B. cornutus released in the natural reef was 6.2 cm in mean shell height, and 58.9 g in mean shell weight. The size of B. cornutus released in the artificial reef was 6.6 cm in mean shell height, and 65.9 g in mean shell weight. During the release period, most of B. cornutus were not moved much (less than 10 m) from the original release sites. When B. cornutus was recaptured in October after 7 months, the size of B. cornutus released in the natural reef became 7.4 cm in mean shell height, and 89.4 g in mean shell weight. The size of B. cornutus released in the artificial reef became 7.2 cm in mean shell height, and 84.9 g in mean shell weight. This indicates that the growth rate of B. cornutus released in the natural reef was higher than that of B. cornutus in the artificial reef. These differences in the growth of B. cornutus between study sites were ascribed to the abundance of marine algae grazed by immobile B. cornutus. Namely, with relatively high growth rate of B. cornutus in the natural reef, the number of species (23 species) and biomass (26,703.4 g) of algae were more diverse and abundant than those (7 species and 17,018.4 g) of algae in the artificial reef. The growth of B. cornutus in the natural reef was also correlated to high water temperature $(15.5-25.9^{\circ}C)$.

• The Korean Journal of Malacology
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• v.28 no.4
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• pp.321-328
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• 2012

Light and transmission electron microscopy of Batillus cornutus hemocytes revealed differences that the morphological distinctions between blast-like cell, granulocytes and hyalinocytes. Base on the morphological characteristics of the cells, we identified the eight types of hemocytes and present a categorization of the hyalinocytes into six sub-categories. The hemocytes of B. cornutus were observed basophilic cell under the light microscopy. Blast-like cells had a spherical profile with a central nucleus filling almost the whole cell. Granulocytes were characterized by presenting variable numbers of granules. This cell had spherical shape with diameter 7 ${\mu}m$ and smooth endoplasmic reticula, granules, mitochondria, glycogen granules in the cytoplasm. Hyalinocytes were the most abundant cell type. Especially, hyalinocyte VI had iirregular an amoebal shape and observed autophagosome and heterophagosome in the cytoplasm. From these results, it is concluded that there are eight types of cells in the hemolymph of B. cornutus. Further studies are now needed to identify the role of these hemocytes in the enzymological and immunological response.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.299-306
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• 2011

This paper examines relation between growth of adult top shell and their selectivity over different marine algae. For the study the top shell density and the algal species in different depth were surveyed by SCUBA. To test selectivity over different types of algae, five algal species including Ecklonia cava, Undaria pinnatifida, Ulva spp, Gelidium amansii and Sargassum spp were provided to top shells in indoor water tank condition. In the field survey, the wet weight of Ecklonia cava was significantly (r = 0.612, p < 0.05) correlated to the shell length (SL) of top shell. In the indoor water tank experiment conducted over 12 hours after sunrise, adult top shell (SL > SL 4.5 cm) tended to select Ecklonia cava and Undaria pinnatifida, which are common and abundant algal in the subtidal waters in Jeju Island. Our field survey and the indoor experiment suggest that E. cava and U. pinnatifida could be food preferred by adult top shell in its habitat.

• 한국수산학회:학술대회논문집
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• 2005.05a
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• pp.192-193
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• 2005

• 한국수산학회:학술대회논문집
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• 2005.05a
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• pp.196-197
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• 2005

• The Korean Journal of Malacology
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• v.23 no.2
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• pp.209-216
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• 2007

The ovarian structure and ultrastructural changes in germ cells during oogenesis of the spiny top shell, Batillus cornutus, have been investigated by light and electron microscopic observations. Specimens of the spiny top shell were collected from the subtidal zone of Wando, south coast of Korea. Spiny top shell was dioecious. The ovary was located on the surface of the hepatopancreas in the spiral posterior region. The ovary had greenish color in the gonadal mature season. The ovary comprised many ovarian lobules, and the ovarian lobule consisted of connective mesenchymal tissue and epithelial cells. Oogonium had a large nucleus with nucleolus of high electron density. In previtellogenic oocyte, small yolk granules of low electron density were scattered in the cytoplasm. Oocytes in the initial vitellogenic stage were connected with the ovarian lobule by egg stalk. The result of TEM observations showed that initial vitellogenic oocyte contained well-developed Golgi complex, endoplasmic reticula, tubular mitochondria and numerous yolk granules of various electron densities and sizes. The electron density, size and quantity of yolk granules which were distributed in the active vitellogenic oocyte were increased compared to the previous stage. Thickness of egg envelope in the late active vitellogenic oocyte was approximately 4.4 $[\mu}m$. Cytoplasm of ripe oocyte was filled with proteid yolk globules of high electron density and lipid yolk globules of low electron density. In this stage, the thickness of egg envelope was approximately 6.5 ${\mu}m$.

• Development and Reproduction
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• v.11 no.2
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• pp.97-104
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• 2007

This study describes the spermatogenesis and sperm ultrastructure of the spiny top shell, Batillus cornutus using light and electron microscopy. The spiny top shells were collected by divers in the coastal water of Wandogun, Cheollanamdo, Korea(N $34^{\circ}13'$, E $126^{\circ}47'$) at May 2003. Spiny top shells of $60.0{\sim}69.9\;mm$ in shell height were used in this study. The testis comprises many spermatogenic follicles which contains germ cells in different developmental stages. The primary spermatocytes in the pachytene stage were characterized by synaptonemal complexes. The early spermatids were characterized by appearance of Golgi complex, increased karyoplasmic electron density and tubular mitochondria. In early spermatid the mass of proacrosomal granules consists of numerous heterogeneous granules with high electron density. From the mid-stage of spermiogenesis the well-developed mitochondria aggregate posterior to the nucleus, and surround the proximal and distal centrioles. In this stage, proacrosomal granules are condensed and form a acrosome with thin envelope. During the late spermiogenesis, the acrosome begins to elongate and then became conical. The sperm consists of head, mid-piece and tail. The head comprises a round nucleus and a conical acrosome. Acrosomal rod of microfibrous is observed between nucleus and acrosome. Five mitochondria observed in mid-piece. And tail has the typical "9+2" microtubular system originates from the centrioles.

• 한국수산학회:학술대회논문집
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• 2005.05a
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• pp.194-195
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• 2005