• Journal of Life Science
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• 제11권2호
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• pp.116-119
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• 2001

Xinlei studies on the production of pullulan by Aureobasidium pullulans using batch culture in a 15L bioreactor were carried out. The mathematical models were obtained in this study, which provided a reasonable description for the biomass, the product, and the substrate variation with time. The values frets the mathematical models were satisfactorily coincided with the experimental data for the biomass of A. pullulans, the production of pullulan and the utilization of sucrose as the sole carbon source.

• 한국축산식품학회지
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• 제37권1호
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• pp.147-152
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• 2017

A bioluminescent Lactobacillus plantarum (pLuc2) strain was constructed. The luminescent signal started to increase during the early exponential phase and reached its maximum in the mid-exponential phase in a batch culture of the strain. The signal detection sensitivity of the strain was the highest in PBS (phosphate buffered saline), followed by milk and MRS broth, indicating that the sensitivity was influenced by the matrix effect. The strain was used in millet seed fermentation which has a complex matrix and native lactic acid bacteria (LAB). The luminescent signal was gradually increased until 9 h during fermentation and abolished at 24 h, indicating that the strain could be specifically tracked in the complex matrix and microflora. Therefore, the bioluminescent labeling system can be used for monitoring LAB in food and dairy sciences and industries.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2005년도 International Symposium & conference of the Plant Resources Society of Korea
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• pp.11-16
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• 2005

Utilization of herbal medicine has been an intrinsic part of Philippine culture for many centuries. In spite of tremendous development in the field of modern medicine, traditional and alternative medicine will continue to flourish and take an important role in meeting the basic health needs of the people. The National Integrated Research Program on Medicinal Plant (NIRPROMP), the lead government institution for herbal medicinal research had already validated ten medicinal plants and is currently studying new batch of medicinal plants. Five of the ten clinically-tested medicinal plants have been elevated to herbal medicinal drugs that are now being commercially manufactured in the different parts of the country. Research on medicinal plants takes longer time as the nature of medical research dictates it to be. It must adhere to the internationally accepted standards of medicinal research in the Philippines was observed making alternative medicine an effective health delivery system to the people.

• 약학회지
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• 제39권3호
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• pp.306-313
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• 1995

The aim of the present study was to produce low molecular weight cathepsin B inhibitor. A strain of Streptomyces aburabiensis isolated from soil in Korea was selected and the optimum condition for the production of the inhibitor was evaluated. Glucose and soytone were selected as best carbon and nitrogen sources, respectively. From the kinetic analysis in batch fermentation, it was found that the specific cathepsin B inhibitor production rate (q$_{p}$) was linearly related to specific growh rate ($\mu$). The inhibitor in culture filtrate was purified by adsorption on activated charcoal, butanot extraction, silica gel chromatography, ion exchange chromatography using Dowex-1 (Cl form) and Amberlite IRC-50 (H$^{+}$ form), and preparative TLC. From the UV, IR, Mass spectroscopy and $^{1}$H-NMR, the inhibitor was thought to be a new inhibitor of which molecular weight was 199.

• KSBB Journal
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• 제19권2호
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• pp.143-147
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• 2004

휘발성 황화합물 중 생물학적으로 가장 난분해성으로 알려 져 있는 DMS에 대한 분해능이 우수한 신균주를 분리. 동정 하여 Gordonia sihwaniensis PKL-1로 명명하였다. 이 균주는 D DMS 초기농도가 35 mg/L 이상일 때에도 우수한 분해능을 보였고, 회분식 배양에서 최대 비분해속도 $\upsilon_{max}$ 없는 0.0016 mg/(mg-protein).hr이 었고, 최적 의 온도와 pH는 각각 $25^{\circ}C$ 와 pH는 7이였다.

• KSBB Journal
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• 제15권6호
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• pp.644-648
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• 2000

Biological fixation of carbon dioxide using microalgae is known as an effective CO$_2$reduction technology. However, many environmental factors influence microalgal productivity. Optimal cultivation factors were determined for the green alga, Euglena gracilis Z, which offers high protein and vitamin E content for animal fodder. In batch culture in a photovioreactor, it was found that theinitial pH, temperature, CO$_2$concentration in air, and light intensity during the optimal cultivating conditions were 3.5, 27$^{\circ}C$, 5-10% and 520 ${\mu}$mol/㎡/s, respectively. When tap water and freshwater were used as cultivating media unsterilized tap water was found to be effective. A kinetic model was considered to determine the relationship between the specific growth rate and the light intensity. The half-velocity coefficient (K(sub)I) in the Monod model under photoautotrophic conditions was 978.9 ${\mu}$mol/㎡/s.

• 한국환경보건학회지
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• 제31권4호
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• pp.327-331
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• 2005

Micromonosporas purpurea로부터 효율적 gentamicin 생산을 위해 protoplast fusion와 protoplast mutagenesis 방법이 검토 되었다. $CO^{60}\;irradiation\;(2.3{\times}10^5$ units, UV 3 min) 방법에 의해서 MP3-112, MP3-141, MP3-143을 분리 했다. 특히 MP3-143균주는 최대 gentamicin생산량이 얻어졌다. 개량된 MP3-143균주를 이용해서 탄소원 소비, 균체성장, 그리고 gentamicin 생산량이 batch culture에서 비교되었다. MP3-413와 parent 균주의 glucose 소비는 배양 2일과 3일 후에 각각 완전히 이루어졌다. 그러나 균체성장과 Soybean oil 소비는 비슷한 결과 얻어졌다. Gentamicin최대 생산량은 배양 5일 후 29756 U/ml였다. 이 결과는 parent 균주에 비해 생산량이 5.6배 증가했다.

• Journal of Microbiology and Biotechnology
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• 제10권5호
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• pp.628-631
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• 2000

The timing of poly(3-Hydroxybutyrate) (PHB) biosynthesis was controlled by varying the agitation speed of a stirred tank fermentor during the pH-stat fed-batch culture of recombinant Escherichia coli strain GCSC 6576 harboring pSYL107. Using a concentrated whey solution containing ca. 200 g/l lactose as the nutrient feed, the PHB content was only 57% after 35h due to volumetric limitation of the fermentor. However, by limiting the oxygen by maintaining the agitation speed at 300 rpm, the final PHB content increased to 70% after 70h with a cell concentration of 15 g/l. When the agitation speed was increased up to 500 rpm, a cell concentration of 31 g/l with 80% PHB was obtained after 52h. A further increase in the maximum agitation speed increased the cell concentration, PHB concentration, and PHB productivity, however, the PHB content decreased to 56-58%.

• 한국방재학회:학술대회논문집
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• 한국방재학회 2008년도 정기총회 및 학술발표대회
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• pp.811-813
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• 2008

To see the effect of magnesium on adhesion to natural zeolites, a series of batch tests were performed in this research. Mixed bacteria were sampled from the digestion tank at a local sewage treatment plant in Seoul. Magnesium-zeolites were synthesized by mixing natural zeolites with 0.096 M, 0.24 M, and 0.48 M of MgCl2 solution. For comparison, manganese and trivalent ferric zeolites were also prepared. Two grams of 0.2 mm $\sim$ 0.3 mm sized zeolites(non-treated, Mg, Mn and Fe(III) treated zeolites) and 20 mL of water were mixed in a Corex 25 mL tube. Five milliliters of culture solution including bacteria was added to the tube. The tubes were equilibrated in a shaking incubator at mesophilic temperature $(30{\pm}2^{\circ}C)$. The bacterial concentrations were measured with a Microluminometer (New Horizons 3550i) and total organic carbon (TOC) spectrophotometer (Multi NC-3100).

• KSBB Journal
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• 제7권4호
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• pp.247-251
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• 1992

회전식 진탕 배양기를 사용한 회분식 발효실험을 통하여 효모 S. cerevisiae ATCC 24858의 균체농도 변화가 비성장 속도, 균체수율, 에탄올수율, 에탄올 생산성 등에 미치는 영향을 규명하여 보았다. 균체의 비성장속도는 균체농도가 증가함에 따라 감소하여 균체농도가 55g/L에 이르게 되면 $Ohr^{-1}$에 도달하여 균체성장이 멈추게 되었다. 에탄올수율 $Y_{p/s}$는 균체농도 변화에 관계없이 일정한 값 0.43을 나타내었으나 균체수율$Y_{x/s}$는 균체농도 증가에 EK라 감소 경향을 나타내어 균체농도55g/L에서는 그 값이 0을 나타내었다. 에탄올 생산성은 균체농도 증가에 따라 선형적인 함수관계로 증가하는 경향을 나타내어 초기 당농도 170g/L, 초기 균체농도55g/L인 경우에는 30g/L까지 상승하였다. 또한 본 연구의 실험 결과를 Luong식에 적용하여 군체성장이 멈추는 에탄올의 농도가 95g/L임을 알 수 있었다.