• 한국식품과학회지
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• 제29권1호
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• pp.72-76
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• 1997

압출성형기의 모터에너지는 자가발열 열에너지와 기계에너지로 전환되며 히터에너지는 열에너지로 공급되는데 각 에너지가 녹말의 호화에 미치는 영향을 분석하였다. 저온$({\leq}80^{\circ}C)$ 압출성형조건에서 자가발열 에너지는 낮은 수분함량의 분체 마찰에 의한 것과 높은 수분함량의 점성에 의한 것으로 구분할 수 있었으며 분체 마찰에 의한 열에너지가 호화에 더 효과적임을 알 수 있었다. 또한 모터에너지에서 자가발열되고 남은 순수한 기계에너지의 효과를 상대기계에너지로 평가한 결과, 높은 수분함량$({\geq}33%)$ 조건의 호화는 상대기계에너지의 변화에 크게 의존하며 낮은 수분함량$({\leq}30%)$의 경우는 거의 영향을 받지 않는 것으로 나타났다.

• 한국군사과학기술학회지
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• 제4권2호
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• pp.256-262
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• 2001

Viscous solutions of supersonic side jet nozzle and supersonic jet impinging on a flat plate are simulated using three-dimensional Navier-Stokes solver. For rapid and abrupt control of a missile in supersonic flight, side jet on a missile body is found to be a useful devise as evidenced by recent missile development at several nations. The magnitude of the side jet and the duration of it decide the level of control of such a missile system. The aerodynamic characteristics of the side jet devise itself are examined in terms of key parameters such as the side jet nozzle geometry, the chamber pressure and temperature. On the other hand, the jet impinging flow structure exhibits such complex nature as shock shell, plate shock and Mach disk depending on the flow parameters. Among others, the dominant parameters are the ratio of the nozzle exit pressure to the ambient pressure and the distance between the nozzle exit plane and the impinging plane. As the plate is placed close to the nozzle, the computed wall pressure at or near the jet center oscillates with large amplitude with respect to the mean value. The amplitude of wall pressure fluctuations subsides as the plate/nozzle distance increases, and the frequency of the wall pressure is estimated on the order of 10.0 KHz. Objectives of this paper are to show accurate simulation of nozzle flow itself and to demonstrate the jet flow structure when the jet interacts with a wall at a close range.

• 한국식품과학회지
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• 제52권4호
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• pp.357-362
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• 2020

본 연구는 조직감 및 기능성 면에서 우수한 식물성 대체육의 제조를 위하여 부원료로 버섯을 첨가한 압출성형 대체육의 품질특성에 대하여 살펴보았다. 대체육의 원료는 분리대두단백 50%, 글루텐 40%, 옥수수 전분 10%를 섞은 것을 기본배합(base)으로 하였다. 버섯 첨가함량을 기본배합 대비 0, 4, 8, 12%로 한 총 네개의 시료를 수분함량 55%, 스크루 속도 150 rpm, 바렐 온도를 170℃로 하여 냉각 사출구를 통해 20℃로 사출하였다. 압출성형 후 물리적 특성으로 경도, 탄성, 응집성, 씹힘성과 절단 강도, 조직잔사지수, 보수력을 측정하였고, 화학적 특성으로 수용성 질소지수, 단백질 소화율과 DPPH 라디칼 소거활성을 측정하였다. 버섯 첨가함량이 증가함에 따라 경도, 탄성, 응집성, 씹힘성과 절단강도가 모두 증가하였으며, 조직잔사지수 역시 증가하였다. 반면에, 버섯 첨가함량이 증가할수록 보수력과 수용성 질소지수는 감소하였으며, 단백질 소화율도 다소 감소하였다. DPPH 라디칼 소거활성은 버섯 첨가함량의 증가에 따라 유의적인 증가를 나타내었다. 압출성형 대체육에 버섯의 첨가는 대체육의 조직감을 개선하고 항산화 기능성을 부여하는 유효한 공정임을 확인하였다.

• 한국식품영양학회지
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• 제13권6호
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• pp.547-552
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• 2000

옥수수에 5 $\mu$g/g의 FB$_1$을 인위적으로 첨가한 후 co-rotating mixing twin screw extruder를 사용하여 140, 160, 및 18$0^{\circ}C$에서 각각 screw speed를 80, 100 및 120rpm으로 조정하여 extrusion 처리하였다. Extrusion 처리에 의한 FB$_1$ 감소율은 약 41~45% 수준이었으며, 처리 조건에 따른 차이는 없었다. Glucose, fructose 및 sucrose를 각각 첨가한 옥수수를 14$0^{\circ}C$, 120 rpm에서 extrusion 처리하였을 때 glucose를 첨가한 옥수수에서의 FB$_1$ 감소율이 증가하였다. Glucose 함량의 증가에 따라 FB$_1$의 감소율도 증가하였으며, glucose가 2.5%, 5% 및 7.5% 존재할 때 extrusion 처리 옥수수에 잔존하는 FB$_1$은 각각 51%, 34% 및 19%수준이었다.

• 신재생에너지
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• 제6권4호
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• pp.6-14
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• 2010

Pretreatment of cellulosic biomass is necessary before enzymatic saccharification and fermentation. Extrusion is a well established process in food industries and it can be used as a physicochemical treatment method for cellulosic biomass. Aqueous ammonia soaking treatment at mild temperatures ranging from 60 to $80^{\circ}C$ for longer reaction times has been used to preserve most of the cellulose and hemicellulose in the biomass. The objective of this study was to evaluate the effect of extrusion treatment on aqueous ammonia soaking method. Extrusion was performed with miscanthus sample conditioned to 2mm of particle size and 20% of moisture content at $200^{\circ}C$ of barrel temperature and 175rpm of screw speed. And then aqueous ammonia soaking was performed with 15%(w/w) ammonia solution at $60^{\circ}C$ for 1, 2, 4, 8, 12 hours on the extruded and raw miscanthus samples respectively. In the combined extrusion-soaking treatment, most compositions removal occurred within 1~2 hours and on a basis of 1 hour soaking treatment values, cellulose was recovered about 85% and other compositions, including hemicellulose, are removed about 50% from extruded miscanthus sample. The combined extrusion-soaking treated and soaking only treated samples were subjected to enzymatic hydrolysis using cellulase and ${\beta}$-glucosidase. The enzymatic digestibility value of combined extrusion-2 hours soaking treated sample was comparable to 12 hours soaking only treated sample. It means that extrusion treatment can shorten the conventional long reaction time of aqueous ammonia soaking. The findings suggest that the combination of extrusion and soaking is a promising pretreatment method to solve both problems for no lignin removal of extrusion and long reaction time of aqueous ammonia soaking.

• 한국식품조리과학회지
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• 제28권6호
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• pp.813-820
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• 2012

To develop the high quality gluten-free rice products with health functionality and desirable texture with moistness, the physicochemical properties of extruded rice flours prepared from the mixture of germinated brown and white rices were investigated. The domestic organic Samgwangbyeo was used to make white and germinated brown rices. White rice (WR) was dried after soaked for 6 h at $15{\pm}3^{\circ}C$ and mixed with germinated brown rice (GBR) with different mixing ratios (100:0, 75:25, 50:50, 25:75, 0:100). The operating conditions of twin screw extruder were 250 rpm of screw speed, $120^{\circ}C$ of barrel temperature, and 25% moisture content of rice flour. The ash, crude protein and crude lipid contents were significantly different (p<0.05) and those of extruded GBR were the highest values, but those of extruded WR were the lowest. The color difference of extruded WR based on white plate showed the lowest among them. The water binding capacity (334.16%), swelling power (8.83 g/g), solubility (33.13%), and total starch (79.50%) were the lowest in extruded GBR. The viscosities of all extruded rice flours by RVA were maintained during heating. The peak and total setback viscosities of extruded rice flours ranged 127-352 and 58.0-85.5 cP, respectively. The novel food biomaterial from germinated brown rice as well as white rice was developed by twin screw extruder. The extruded rice flours control the moistness to improve the texture and also have functional materials, dietary fiber, GABA, and ferulic acid, etc to increase quality of gluten free rice products.

• 한국항공우주학회지
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• 제30권1호
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• pp.114-123
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• 2002

케로신/액체산소 추진기관을 갖는 초음속 로켓의 플룸 유동장을 9 화학종 14 반응 모델과 연계된 레이놀즈 평균 Navier-Stokes 방정식을 이용하여 해석하였다. 유한속도 화학반응이 플룸 유동장에 미치는 영향을 고찰하기 위하여 그 결과를 화학적 동결유동 해석 결과와 비교하였다. 계산은 상용 CFD 소프트웨어인 FLUENT 5를 이용하여 수행하였다. 반응 유동 해석 결과는 노즐 내부에서의 화학반응에 따른 연소가스의 온도 증가로 인해 전체적으로 동결유동에 비해 더 높은 온도장을 나타내었다. 플룸에서의 모든 화학반응은 전단류와 배럴 충격파 반사지점 후방의 고온 영역에 국한되어 일어났으며 본 해석의 경우 플룸내에서의 유한속도 화학반응이 유동에 미치는 영향은 미약한 것으로 나타났다. 그러나 본 연구에서 이루어진 유한속도 화학반응을 고려한 플룸 해석을 통하여 플룸에서의 주된 화학 반응 및 이들의 반응 메커니즘을 확인할 수 있었다.

• 한국식품과학회지
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• 제41권4호
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• pp.369-373
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• 2009

전분액화에 호화찰옥수수전분가 쌍축형 압출성형의 원료로 직접 사용되었다. 그 액화 효과와 체류시간분포를 분석하였다. 내열성 ${\alpha}$-amylase(Bacillus licheniformis로부터 분리)를 함께 첨가하여 사용하였다. 전분액화는 환원당측정, gel permeation chromatography(GPC), 주사전자현미경을 통하여 분석하였다. 배럴온도와 수분함량이 높을수록 환원당 함량은 증가하였고, 호화전분의 경우 생전분보다 더 많은 환원당이 생성되었다. GPC에서 호화전분의 사용에 의한 저분자화 효과는 뚜렷하지 않았으나, 효소 첨가없이 압출성형하는 경우에는 일부 효과가 있는 것으로 나타났다. 주사전자현미경의 미세구조에서는 호화전분을 쓸 경우 그 표면이 더 불규칙하고 침식되는 것을 관찰할 수 있었다. 종합적으로 호화전분의 사용이 전분액화에 효과가 있음을 알 수 있었다. 체류시간분포에서는 호화전분을 원료로 할 경우 그 분산도가 커지는 것으로 나타났다. 이는 호화전분은 압출성형의 흐름이 원만하지 않은 것을 의미하였으나, 배럴온도와 수분함량을 증가시킴에 따라 그 분산도를 감소시킬 수 있음을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제29권2호
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• pp.244-255
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• 2019

Xylose isomerase (XI; E.C. 5.3.1.5) catalyzes the isomerization of xylose to xylulose, which can be used to produce bioethanol through fermentation. Therefore, XI has recently gained attention as a key catalyst in the bioenergy industry. Here, we identified, purified, and characterized a XI (PbXI) from the psychrophilic soil microorganism, Paenibacillus sp. R4. Surprisingly, activity assay results showed that PbXI is not a cold-active enzyme, but displays optimal activity at $60^{\circ}C$. We solved the crystal structure of PbXI at $1.94-{\AA}$ resolution to investigate the origin of its thermostability. The PbXI structure shows a $({\beta}/{\alpha})_8$-barrel fold with tight tetrameric interactions and it has three divalent metal ions (CaI, CaII, and CaIII). Two metal ions (CaI and CaII) located in the active site are known to be involved in the enzymatic reaction. The third metal ion (CaIII), located near the ${\beta}4-{\alpha}6$ loop region, was newly identified and is thought to be important for the stability of PbXI. Compared with previously determined thermostable and mesophilic XI structures, the ${\beta}1-{\alpha}2$ loop structures near the substrate binding pocket of PbXI were remarkably different. Site-directed mutagenesis studies suggested that the flexible ${\beta}1-{\alpha}2$ loop region is essential for PbXI activity. Our findings provide valuable insights that can be applied in protein engineering to generate low-temperature purpose-specific XI enzymes.

• Fisheries and Aquatic Sciences
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• 제25권3호
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• pp.175-186
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• 2022

Sea mustard (Undaria pinnatifida), an important edible seaweed belonging to the brown algal family of Alariaceae, contains copious physiologically active substances. It has long been popular in Korea as a food and is frequently consumed in the form of soup. It is also commercially available as a home meal replacement. In this study, we developed a seasoning key base with a high degree of sensory preference from sea mustard using the extrusion cooking process. Extrusion cooking conditions were optimized through response surface methodology. Barrel temperature (X₁, 140℃-160℃) and screw speed (X₂, 158-315 rpm) were set as independent variables, and overall preference was determined as the dependent variable (Y, points). An optimal condition was obtained at X₁ = 148.5℃ and X₂ = 315 rpm, and the dependent variable (Y, overall acceptance) was 7.95 points, similar to the experimental value of 7.81. Umami taste had a relationship with the overall acceptance of sea mustard seasoning. In the electronic nose and tongue, increased sourness and umami intensities were associated with the highest sensory score. The samples were separated well by each characteristic via principal component analysis. Collectively, our study provides imperative preliminary data for the development of various seasonings using sea mustard.