• 한국응용곤충학회지
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• 제55권3호
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• pp.309-312
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• 2016

그 동안 국내 Drepanepteryginae 아과(낙엽날개뱀잠자리붙이아과; 신칭)에는 오직 Neuronema albostigma만이 기록되어 있었으나, 이번 연구를 통해 국내 미기록속인 Drepanepteryx와 이 속에 포함되는 미기록종 Drepanepteryx phalaenoides (낙엽날개뱀잠자리붙이; 신칭)를 새롭게 보고한다. 이 종에 대한 간략한 기재와 속내 분류키, COI 바코드 염기서열, 그리고 성충 및 수컷 생식기의 사진을 제공한다.

• ALGAE
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• 제33권2호
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• pp.167-180
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• 2018

Benthic diatoms are ubiquitous in tidal flats and play major roles in maintaining coastal ecosystems. Spatio-temporal variations in diatom diversity have not been well-studied, mainly because of difficulties in morphological identification and the lack of appropriate genetic tools. To overcome these problems, we used the gene encoding the ribulose bisphosphate carboxylase large-subunit (rbcL) as a molecular marker, and sequenced these genes with the aid of the MiSeq platform. In this manner, we explored the genetic diversity of benthic diatoms in tidal flats of Guenso Bay on the west coast of Korea; differences in the spatial distributions of benthic diatoms were evident. The diatom communities were dominated by Nitzschia, Navicula, and Amphora; their relative distributions were affected by the sand proportion, grain size, and air exposure time. Our results suggest that meta-barcoding of the rbcL gene and next-generation sequencing can be used to explore the diversity of benthic diatoms.

• Animal Systematics, Evolution and Diversity
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• 제27권2호
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• pp.159-163
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• 2011

DNA barcoding of Bryozoa or "moss animals" has hardly advanced and lacks reference sequences for correct species identification. To date only a small number of cytochrome c oxidase subunit I (COI) sequences from 82 bryozoan species have been deposited in the National Center for Biotechnology Information (NCBI) GenBank and Barcode of Life Data Systems (BOLD). We here report COI data from 53 individual samples of 29 bryozoan species collected from Korean seawater. To our knowledge this is the single largest gathering of COI barcode data of bryozoans to date. The average genetic divergence was estimated as 23.3% among species of the same genus, 25% among genera of the same family, and 1.7% at intraspecific level with a few rare exceptions having a large difference, indicating a possibility of presence of cryptic species. Our data show that COI is a very appropriate marker for species identification of bryozoans, but does not provide enough phylogenetic information at higher taxonomic ranks. Greater effort involving larger taxon sampling for the barcode analyses is needed for bryozoan taxonomy.

• 생태와환경
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• 제48권4호
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• pp.280-286
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• 2015

점봉산의 한 소하천에 서식하는 녹색강도래 Sweltsa illiesi와 S. lepnevae의 생활환이 추정되었다. 약충은 DNA barcode로 동정되었다. 이들의 약충은 성충으로 우화하기 몇 달 전부터 머리에 종 특이적 무늬가 나타났으며, 더듬이 셋째 마디의 모양이 서로 달랐다. 두 종은 거의 동일한 semivoltine 생활환을 가지며, 산란된 알은 6월에 부화하여 약 701일의 생육기간을 보내는 것으로 나타났다. 두 종의 통합된 연평균 생물량은 회분외질량 (AFDM)으로 $96mg\;AFDM\;m^{-2}$으로 추정되었다. 통합 2차생산력은 size frequency법으로는 373 mg, increment summation법으로는 $297mg\;AFDM\;m^{-2}\;yr^{-1}$인 것으로 추정되었다.

• Molecules and Cells
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• 제23권2호
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• pp.220-227
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• 2007

The development of suitable genetic markers would be useful for defining species and delineating the species boundaries of morphologically indistinguishable sponges. In this study, genetic variation in the sequences of nuclear rDNA and the mitochondrial cytochrome c oxidase subunit 1 and 3 (CO1 and CO3) regions were compared in morphologically indistinguishable Korean Halichondriidae sponges in order to determine the most suitable species-specific molecular marker region. The maximal congeneric nucleotide divergences of Halichondriidae sponges in CO1 and CO3 are similar to those found among anthozoan cnidarians, but they are 2- to 8-fold lower than those found among genera of other triploblastic metazoans. Ribosomal internal transcribed spacer regions (ITS: ITS1 + ITS2) showed higher congeneric variation (17.28% in ITS1 and 10.29% in ITS2) than those of CO1 and CO3. Use of the guidelines for species thresholds suggested in the recent literature indicates that the mtDNA regions are not appropriate for use as species-specific DNA markers for the Halichondriidae sponges, whereas the rDNA ITS regions are suitable because ITS exhibits a low level of intraspecific variation and a relatively high level of interspecific variation. In addition, to test the reliability of the ITS regions for identifying Halichondriidae sponges by PCR, a species-specific multiplex PCR primer set was developed.

• Mycobiology
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• 제45권3호
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• pp.139-149
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• 2017

The genus Hyaloperonospora (Peronosporaceae; Oomycota) is an obligate biotrophic group that causes downy mildew disease on the Brassicaceae and allied families of Brassicales, including many economically relevant crops, such as broccoli, cabbage, radish, rape, and wasabi. To investigate the diversity of Hyaloperonospora species in northeast Asia, we performed a morphological analysis for the dried herbarium specimens collected in Korea, along with molecular phylogenetic inferences based on internal transcribed spacer rDNA and cox2 mtDNA sequences. It was confirmed that 14 species of Hyaloperonospora exist in Korea. Of these, three species, previously classified under the genus Peronospora, were combined to Hyaloperonospora: H. arabidis-glabrae comb. nov. (ex Arabis glabra), H. nasturtii-montani comb. nov. (ex Rorippa indica), and H. nasturtii-palustris comb. nov. (ex Rorippa palustris). In addition, finding two potentially new species specific to northeast Asian plants is noteworthy in support of the view that the species abundance of Hyaloperonospora has been underestimated hitherto.

• Fisheries and Aquatic Sciences
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• 제21권3호
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• pp.8.1-8.6
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• 2018

Desmarestia japonica (Phaeophyceae, Desmarestiales) was recently established from the Japanese ligulate Desmarestia and is morphologically similar to D. ligulata. This species has been reported only from Japan. However, the taxonomic reports based on additional regional distributions are needed to clarify this taxonomic entity and its species boundaries. Because Desmarestia species have restricted distributions in Korea, we reexamined herbarium specimens of D. ligulata deposited at the National Institute of Biological Resources (South Korea). To improve the amplification efficiency of the polymerase chain reaction and avoid contamination by the DNA of other organisms, we developed taxon-specific molecular markers suitable for DNA barcoding of Desmarestia species. Nuclear ribosomal small subunit RNA (18S rDNA) and mitochondrial cytochrome c oxidase 1 (cox1) regions were selected as target DNA. As a result, both were successfully isolated from herbarium specimens of D. japonica acquired over 10 years. These molecular markers provide useful genetic information for herbarium specimens for which conventional molecular analysis is challenging.

• Mycobiology
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• 제45권4호
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• pp.263-269
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• 2017

The genus Peronospora, an obligate biotrophic group belonging to Oomycota, causes serious damage to a variety of wild and ornamental plants, as well as cultivated crops, such as beet, rose, spinach, and tobacco. To investigate the diversity of Peronospora species parasitic to Stellaria and Pseudostellaria (Caryophyllaceae) plants in Korea, we performed a morphological analysis on dried herbarium specimens and molecular phylogenetic inferences based on internal transcribed spacer rDNA and cox2 mitochondrial DNA sequences. As a result, it was confirmed that there are four species of Peronospora parasitic to specific species of Stellaria and Pseudostellaria, all of which were hitherto unrecorded in Korea: P. alsinearum (ex Stellaria media), P. stellariae-aquaticae (ex Stellaria aquatica), P. stellariae-uliginosae (ex Stellaria alsine), and P. pseudostellariae (ex Pseudostellaria palibiniana). In addition, Peronospora specimens parasitic to Pseudostellaria davidii differed morphologically from P. pseudostellariae owing to the large and ellipsoidal conidia; this morphological discrepancy was also validated by the high genetic divergence between the two species. Peronospora casparyi sp. nov. is described and illustrated here.

• ALGAE
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• 제32권1호
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• pp.29-39
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• 2017

Geographic distributions of pathogens are affected by dynamic processes involving host susceptibility, availability and abundance. An oomycete, Pythium porphyrae, is the causative agent of red rot disease, which plagues Pyropia farms in Korea and Japan almost every year and causes serious economic damage. We isolated an oomycete pathogen infecting Pyropia plicata from a natural population in Wellington, New Zealand. The pathogen was identified as Pythium porphyrae using cytochrome oxidase subunit 1 and internal transcribed spacer of the rDNA cistron molecular markers. Susceptibility test showed that this Pythium from New Zealand was able to infect several different species and genera of Bangiales including Pyropia but is not able to infect their sporophytic (conchocelis) phases. The sequences of the isolated New Zealand strain were also identical to Pythium chondricola from Korea and the type strain from the Netherlands. Genetic species delimitation analyses found no support for separating P. porphyrae from P. chondricola, nor do we find morphological characters to distinguish them. We propose that Pythium chondricola be placed in synonymy with P. porphyrae. It appears that the pathogen of Pyropia, both in aquaculture in the northern hemisphere and in natural populations in the southern hemisphere is one species.

• 한국균학회지
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• 제46권4호
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• pp.375-382
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• 2018

Members of the genus Mortierella (Mortierellales) are filamentous fungi, which are found on nearly all substrates, but more frequently in soil. Till date, 7 species of Mortierella have been reported in Korea, but being a ubiquitous group with high species diversity in temperate zones, this number is still low. During a survey of fungal biodiversity in Korea, we collected many isolates of Mortierella, and through morphological and molecular phylogenetic analyses, identified them to be 3 previously unrecorded species, namely, M. chienii, M. epicladia, and M. gamsii. A total of 10 Mortierella species in Korea, including the 3 species reported in the present study, are widely distributed in 5 out of 7 phylogenetic groups of this genus. This indicates that the diversity of Mortierella was so far underestimated in Korea. Multi-locus sequence analysis is required to provide a more reliable backbone for some uncertain phylogenetic groupings and to more clearly define a species of Mortierella, which would encourage deeper research in the diversity and ecological roles of Mortierella and allied genera.