• 식물분류학회지
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• 제46권2호
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• pp.163-174
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• 2016

Gnetum (Gnetaceae, Gnetales) is a gymnosperm genus with ca. 35 species distributed in tropical forests around the world. Due to its dioecious habit and lack of diagnostic characters from vegetative tissue, the identification of Gnetum species is not easy without seeds or reproductive structures. To identify and verify their phylogenetic positions, we applied DNA barcoding to Cambodian Gnetum collections gathered between 2010 and 2015, with previously designed cp matK gene primers. We newly sequenced partial matK sequences from 72 Gnetum collections, 43 out of 72 from Cambodia, and analyzed 115 Gnetum accessions using the neighbor-joining method. The resulting neighbor-joining tree categorized Cambodian Gnetum samples into three clades of species: G. macrostachyum, G. montanum, and G. aff. gracilipes. The recognition of G. aff. gracilipes in Cambodia is reported here for the first time. Taxonomic information for the three recognized Cambodian Gnetum species is provided and the benefits of the taxonomic reevaluation assisted by DNA barcoding are emphasized in this work.

• 한국토양동물학회지
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• 제11권1_2호
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• pp.106-109
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• 2006

Amynthas hupeiensis with 6/7/8/9 intersegmental spermathecal pores keys to the sieboldi-group in Sims and Easton (1972). Two pairs of round genital papillae are always between segments 17/18 and 18/19, and male pore regions are positioned in between the genital papillae. Korean Amynthas hupeiensis is usually collected from various agroecosystems. Description of the Amynthas hupeiensis is provided, including illustrations of ventral view and spermathecae, DNA barcoding data, and photo.

• Journal of Plant Biotechnology
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• 제49권1호
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• pp.2-2
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• 2022

• 식물분류학회지
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• 제43권4호
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• pp.252-262
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• 2013

캄보디아 캄폿주 프놈보콜국립공원에 대한 식물상 조사 중 열대성 전기생식물인 B. fungosa var. indica를 발견하였다. 이들의 숙주를 확인하기 위해 숙주의 뿌리와 더불어 주변에 위치한 목본 식물을 채집하였으며, 이들을 DNA barcoding 방법을 사용하여 동정하였다. DNA barcode 마커로는 엽록체 rbcL 및 matK 유전자 구간을 적용하였으며, 15개의 숙주 뿌리와 7개의 주변 목본식물로부터 성공적으로 PCR 증폭 및 염기서열을 확보하였다. 숙주 뿌리로부터 얻어진 숙주의 염기서열은 앵초과, 노박덩굴과, 도금양과, 그리고 물푸레나무과로 식별되었으며, 주변의 목본식물은 물푸레나무과, 도금양과, 무환자나무과, 장미과, 물레나물과, 철쭉과와 녹나무과였다. 속 수준에서 앵초과는 Myrsine, 노박덩굴과는 Euonymus, 도금양과는 Syzygium, 물푸레나무과는 Olea 등으로 각각 식별되었으나, 종 수준에서의 동정은 불가능하였다. 앵초과 Myrsine와 물푸레나무과 Olea는 본 연구를 통해 최초로 B. fungosa var. indica의 숙주로 확인되었다. 추가적인 채집 조사 및 비교 연구, DNA barcoding을 통해 해당 지역의 생물다양성과 Balanophora속 식물의 숙주 식물 및 진화에 대해 좀더 명확하게 확인이 가능할 것으로 판단된다.

• Journal of Ecology and Environment
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• 제36권4호
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• pp.449-463
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• 2013

DNA barcoding is becoming a widely applied tool to accurately discriminate red algae. We tested the effectiveness of DNA barcoding for identification and discovery of Champia species in Korea and clarified the phylogenetic relationships using the plastid rbcL gene. As results, we described four species of Champia such as C. inkyua sp. nov., C. recta Noda, C. bifida Okamura, and C. expansa Yendo. A new species, C. inkyua, is characterized by entangled thallus, terete and irregular branches, hooked apices, and longitudinal filaments running throughout the frond periphery only. Longitudinal filaments were composed of a complete cell with two half cells between diaphragms in the cavity. C. recta and C. bifida were reinstated with previously used names of C. parvula and C. compressa, respectively. C. recta is the first recorded species from Korea and is characterized by an erect thallus, terete and irregular branches, and straight apices. C. bifida is characterized by compressed thallus, pinnate or alternate branches, and bifid apices. C. expansa is characterized by flabellate thallus and dichotomous branches. Molecular analyses of COI and rbcL genes revealed sufficient sequence divergence to warrant species recognition in the genus Champia.

• Animal Systematics, Evolution and Diversity
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• 제28권4호
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• pp.269-278
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• 2012

Gobiids are hyperdiverse compared with other teleost groups, with about 2,000 species occurring in marine, freshwater, and blackish habitats, and they show a remarkable variety of morphologies and ecology. Testing the effectiveness of DNA barcodes on species that have emerged as a result of radiation remains a major challenge in evolutionary biology. Here, we used the cytochrome c oxidase subunit 1 (COI) sequences from 144 species of gobies and related species to evaluate the performance of distance-based DNA barcoding and to conduct a phylogenetic analysis. The average intra-genus genetic distance was considerably higher than that obtained in previous studies. Additionally, the interspecific divergence at higher taxonomic levels was not significantly different from that at the intragenus level, suggesting that congeneric gobies possess substantial interspecific sequence divergence in their COI gene. However, levels of intragenus divergence varied greatly among genera, and we do not provide sufficient evidence for using COI for cryptic species delimitation. Significantly more nucleotide changes were observed at the third codon position than that at the first and the second codons, revealing that extensive variation in COI reflects synonymous changes and little protein level variation. Despite clear signatures in several genera, the COI sequences did resolve genealogical relationships in the phylogenetic analysis well. Our results support the validity of COI barcoding for gobiid species identification, but the utilization of more gene regions will assist to offer a more robust gobiid species phylogeny.

• Fisheries and Aquatic Sciences
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• 제26권9호
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• pp.548-557
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• 2023

The purposes of this research were to identify fish species using DNA barcodes or partial sequences of cytochrome b (Cytb) and to assess the diversity of fish in the Mae Tam reservoir, Phayao province, Thailand. Fish samples were collected 3 times, during the winter, summer, and rainy seasons, from 2 sampling sites using gillnets with 3 mesh sizes (30, 50, and 70 mm). A total of 34 representative samples were classified into 12 species, 7 families and 6 orders by morphological- and DNA barcoding-based identifications. However, one cichlid species, Cichlasoma trimaculatum, could only be identified using DNA barcoding. Family Cyprinidae had the greatest diversity, 50.00%. The diversity, richness and evenness indices ranged from 0.43-0.65, 0.64-1.46, and 0.27-0.40, respectively, indicating that fish diversity at both sampling sites was relatively low. A comparison of the catch per unit effort (CPUE) with 3 different mesh sizes found that the 50 mm mesh size was the best (474.80 ± 171.56 g/100 m²/night), followed by the 70 mm (417.41 ± 176.24 g/100 m²/night) and 30 mm mesh sizes (327.88 ± 115.60 g/100 m²/night). These results indicate that DNA barcoding is a powerful tool for species identification. Our data can be used for planning the sustainable management of fisheries resources in the Mae Tam reservoir.

• Fisheries and Aquatic Sciences
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• 제16권1호
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• pp.45-48
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• 2013

A juvenile of the slender fangjaw, Sigmops gracilis G$\ddot{u}$nther, 1878 (Stomiiformes: Gonostomatidae) was collected from Jeju Island, Korea, and identified by DNA barcoding. This species is characterized by its large curved mouth and the presence of 11 dorsal fin rays and 28 anal fin rays. During the juvenile stage, the species is distinguished from other gonostomatid species by the position of the origin of the dorsal fin, which is located at the 7th-8th ray of the anal fin. The Korean name "Sol-ni-ael-tung-i" is proposed for this species.

• Animal Systematics, Evolution and Diversity
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• 제36권3호
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• pp.258-263
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• 2020

The aim of this study is to report monotypic species, Nonparahalosydna pleiolepis(Marenzeller, 1879) for the first time from Korean waters with its DNA barcoding data. We collected individuals of the species from the subtidal zone of southern coast of Korea through scuba diving. To estimate DNA barcoding gap, the pairwise genetic distances were calculated between N. pleiolepis and its congeners (Halosydna brevisetosa Kinberg, 1856 and Lepidonotus squamatus (Linnaeus, 1758)) based on the cytochrome c oxidase subunit I gene (COI). Inter-specific genetic distances ranged from 18.7% to 24.6%, while intra-specific genetic distance within N. pleiolepis ranged from 0.3% to 0.5%. The maximum intra-specific genetic distance among the three species was 1.4%. The morphological diagnosis of N. pleiolepis with a taxonomic note on the species were also provided.

• Animal Systematics, Evolution and Diversity
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• 제35권4호
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• pp.182-185
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• 2019

In this study, cytochrome c oxidase subunit I(COI) sequences of 17 individuals from six Korean diogenid species(i.e., 2 Areopaguristes japonicus, 4 A. nigroapiculus, 3 Paguristes digitalis, 4 P. ortmanni, 3 Diogenes edwardsii, and 1 Ciliopagurus kempfi) were determined and analyzed. The DNA barcoding results of this study were consistent with the morphological identification of these six species. Interspecific variations of COI sequences within six Korean diogenid species exceeded the minimum interspecific variation of diogenid hermit crabs in previous studies. Little intraspecific variation exists except for P. digitalis. This study should facilitate further molecular taxonomy of East Asian diogenids.