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Dermatophytosis of the Four-toed Hedgehog Caused by Trichophyton erinacei

  • Yoon, Ji-Seon;Lee, Jong-Hyun;Yu, Do-Hyeon;Li, Ying-Hua;Lee, Mi-Jin;Iwasaki, T.;Park, Jin-Ho
    • Journal of Veterinary Clinics
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    • v.25 no.3
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    • pp.207-210
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    • 2008
  • Trichophyton erinacei is a dermatophyte pathogen that infects both humans and hedgehogs. A two-month old female four-toed hedgehog presented to the Chonbuk Animal Medical Center with pruritus, excoriation and crust on her face for ten days. The owner of the hedgehog also exhibited the clinical signs of scaly erythema with fine vesicles on her neck. A presumptive diagnosis of dermatophytosis was made based on the results of an acetate tape preparation in which hyphae and chains of arthroconidia were observed. The crusts from the lesions were then cultured on Sabouraud Dextrose Agar for identification. After 10 days of incubation, downy colored colonies that had a central umbo with a white granular surface and a yellow pigment ring in the reverse were observed. Microscopic analysis revealed the presence of numerous teardrop shaped microconidia singly attached to the sides of the hyphae. In addition, 2-6 roomed macroconidia that were somewhat irregular in shape and size were present, and abundant intermediate sized spores were observed between the micro and macro conidia. To confirm that the culture was T. erinacei, the internal transcribed spacer region of the 5.8S phase of the ribosomal RNA gene (ITS1-5.8S-ITS2 rDNA) was amplified by PCR and then sequenced. A 679-base pair fragment of DNA was then compared with sequences in GenBank and found to be 99% homologous with sequences of T. erinacei (Z97997 and Z97996. The clinical signs were resolved after four weeks of treatment with oral and topical ketoconazole and chlorhexidine. To the best of our knowledge, this represents the first case of T. erinacei isolated from a four-toed hedgehog in Korea.

Sedimentology of Inclined Heterolithic Stratification in Sukmo Channel, Kyonggi Bay, Korea - Application to Oil Sand Exploration (경기만 석모수도 수로제방 조간대층에 발달하는 경사이질암상층리의 퇴적학적 연구 - 오일샌드 탐사 적용가능성)

  • Choi, Kyung-Sik;Dalrymple, R.W.;Chun, Seung-Soo;Kim, Sung-Pil;Park, Se-Jin
    • The Korean Journal of Petroleum Geology
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    • v.11 no.1 s.12
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    • pp.18-26
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    • 2005
  • An occurrence of inclined heterolithic stratification (IHS) is described from a tidal point bar in a 40-m-deep distributary of the macrotidai, Han River delta, Korea. The channel bank demonstrates a convex-upward profile with intermittent presence or wave-formed scarps and terraces near the low-water level. The vertical succession of IHS is approximately 25 m thick and dips into the channel with angles reaching up to $14^{\circ}C$. The IHS overlies 15 m of trough cross-bedded sand deposited in the channel bottom. Even though the channel as a whole is ebb dominated, the preserved cross bedding is predominantly flood directed because the mutually evasive nature of the ebb and flood currents causes the point bar surface to be flood dominated. The IHS itself consists of inter-stratified fine sand, sandy silt, and silt with an fining-upward textural trend. Seasonal discharge variations of the Han River are not obvious in the deposits, because tile large size, distal location, and energetic tidal environment of the studied channel reduces the impart of river-stage fluctuations.

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An Analysis on the Economic Impact of ICT Based Innovation within Creative Industries in South Korea (창조산업 내 ICT기반 혁신의 경제적 파급효과 분석)

  • Lee, Youngjoo;Kim, Byungchae;Lee, Yeonwoo
    • Journal of Technology Innovation
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    • v.23 no.3
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    • pp.341-372
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    • 2015
  • While creativity and innovation is the key to drive the creative economy in the South Korea, the development of analysis framework to evaluate the size and performance is limited. The present study suggests a framework and a method to assess economic impact of the creative economy using inter-industry analysis which employs input-output efficiencies populated by the Korean Bank and empirical data from the national informatization survey conducted by the National Information-society Agency(NIA). The results indicated that, as of 2013, despite of economy downturn, the creative innovation based on the information communication technology(ICT) had been significantly led the production, value-added, and employment inducement. The effect is predominant in the creative industry in an broad sense, that is, technology intensive manufacturing industry. Theoretical and policy implications are discussed.

Stem Rot on Ligularia fischeri Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 곰취 흰비단병)

  • Moon, Youn-Gi;Kim, Se-Won;Choi, Jun-Keun;Kwon, Soon-Bae;Shim, Hong-Sik;Ju, Ho-Jong;Choi, In-Young
    • Research in Plant Disease
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    • v.21 no.1
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    • pp.36-39
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    • 2015
  • In June 2012 and 2013, a destructive stem rot symptoms of Ligularia fischeri occurred sporadically in Hoengseong-gun and Pyeongchang-gun Gangwon-do, Korea. The typical symptom included water-soaking on the main stem, rotting, wilting and blighting, which eventually leads to death of the plant. White mycelial mats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotia were white to brown, spherical or irregular, 1-3 mm in size on potato dextrose agar (PDA), The optimum temperature range of hyphal growth was $25-30^{\circ}C$ and the hyphal diameter was $4-10{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. The resulting sequence of 695 bp was deposited in GenBank. A BLAST search revealed that sequences of the this isolates showed >99% identity with those of Sclerotium rolfsii. On the basis of the morphological characteristics and phylogenetic analyses of molecular markers ITS rDNA, the fungi were identified as S. rolfsii. A pathogenicity test was carried out to fulfill Koch's postulates. To our knowledge, this is the first report of S. rolfsii on Ligularia fischeri in Korea.

Characterization of Streptococcus parauberis isolated from cultured Olive flounder, Paralichthys olivaceus in the Jeju Island (제주도 양식넙치 (Paralichthys olivaceus)로부터 분리한 비 용혈성 연쇄구균의 동정)

  • Kang, Chul-Young;Kang, Bong-Jo;Moon, Young-Gun;Kim, Ki-Young;Heo, Moon-Soo
    • Journal of fish pathology
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    • v.20 no.2
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    • pp.109-117
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    • 2007
  • This study was performed to identity non hemolytic streptococcus from cultured flounder (Paralichthys olivaceus) with Streptococcosis in the Jeju island. The result of BIOLOGTM test was Streptococcus uberis that simility of 0.5 and 98% identified in MicroLogTM system (Release 4.05). Carbohydrate utility pattern was dextrin, N-acetyl-D-glucosamine, arbutin, maltose, maltotriose, D-cellobiose, D-fructose, D-mannose, α-D-glucose, D-mannitol, β-methyl D-glucoside, salicin, sucrose, D-trehalose, pruvatic acid methyl ester, mono-methyl succinate, glycerol. In addition hemolysis test for S. parauberis and were S. iniae hemolysis in BAP (Blood agar plate). Antibiotic test for S. parauberis were Ampicillin, Amoxicillin and Fluoroquinolone sensitivity. Mutiplex PCR assay were detected S. pauberis (718 bp), S. iniae (870 bp) L. garviae (1,100 bp). Dectected S. parauberis (718 bp) were result of 16S rRNA sequence identified with S. parauberis (Gene bank accession number X89967). All isolated S. parauberis that with bouned by one group. The result were S. pauberis that γ-hemolytic chain form cocci and negative reaction of catalase, Multiplex PCR assay were 718 bp amplicon size.

Performance Evaluation and Forecasting Model for Retail Institutions (유통업체의 부실예측모형 개선에 관한 연구)

  • Kim, Jung-Uk
    • Journal of Distribution Science
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    • v.12 no.11
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    • pp.77-83
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    • 2014
  • Purpose - The National Agricultural Cooperative Federation of Korea and National Fisheries Cooperative Federation of Korea have prosecuted both financial and retail businesses. As cooperatives are public institutions and receive government support, their sound management is required by the Financial Supervisory Service in Korea. This is mainly managed by CAEL, which is changed by CAMEL. However, NFFC's business section, managing the finance and retail businesses, is unified and evaluated; the CAEL model has an insufficient classification to evaluate the retail industry. First, there is discrimination power as regards CAEL. Although the retail business sector union can receive a higher rating on a CAEL model, defaults have often been reported. Therefore, a default prediction model is needed to support a CAEL model. As we have the default prediction model using a subdivision of indexes and statistical methods, it can be useful to have a prevention function through the estimation of the retail sector's default probability. Second, separating the difference between the finance and retail business sectors is necessary. Their businesses have different characteristics. Based on various management indexes that have been systematically managed by the National Fisheries Cooperative Federation of Korea, our model predicts retail default, and is better than the CAEL model in its failure prediction because it has various discriminative financial ratios reflecting the retail industry situation. Research design, data, and methodology - The model to predict retail default was presented using logistic analysis. To develop the predictive model, we use the retail financial statements of the NFCF. We consider 93 unions each year from 2006 to 2012 to select confident management indexes. We also adapted the statistical power analysis that is a t-test, logit analysis, AR (accuracy ratio), and AUROC (Area Under Receiver Operating Characteristic) analysis. Finally, through the multivariate logistic model, we show that it is excellent in its discrimination power and higher in its hit ratio for default prediction. We also evaluate its usefulness. Results - The statistical power analysis using the AR (AUROC) method on the short term model shows that the logistic model has excellent discrimination power, with 84.6%. Further, it is higher in its hit ratio for failure (prediction) of total model, at 94%, indicating that it is temporally stable and useful for evaluating the management status of retail institutions. Conclusions - This model is useful for evaluating the management status of retail union institutions. First, subdividing CAEL evaluation is required. The existing CAEL evaluation is underdeveloped, and discrimination power falls. Second, efforts to develop a varied and rational management index are continuously required. An index reflecting retail industry characteristics needs to be developed. However, extending this study will need the following. First, it will require a complementary default model reflecting size differences. Second, in the case of small and medium retail, it will need non-financial information. Therefore, it will be a hybrid default model reflecting financial and non-financial information.

Commissioning of a micro-MLC (mMLC) for Stereotactic Radiosurgery (방사선수술용 4뱅크 마이크로 다엽콜리메이터의 인수 검사)

  • Jeong, Dong-Hyeok;Shin, Kyo-Chul;Kim, Jeung-Kee;Kim, Soo-Kon;Moon, Sun-Rock;Lee, Kang-Kyoo
    • Progress in Medical Physics
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    • v.20 no.1
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    • pp.43-50
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    • 2009
  • The 4 bank mico-MLC (mMLC; Acculeaf, Direx, Isral) has been commissioned for clinical use of linac based stereotactic radiosurgery. The geometrical parameters to control the leaves were determined and comparisons between measured and calculated by the calculation model were performed in terms of absolute dose (cGy/100 MU). As a result of evaluating calculated dose for various field sizes and depths of 5 and 10 cm in water in the geometric condition of fixed SSD (source to surface distance) and fixed SCD (source to chamber distance), most of differences were within 1% for 6 MV and 15 MV x-rays. The penumbral widths at the isocenter were approximately evaluated to 0.29~0.43 cm depending on the field size for 6 MV and 0.36~0.51 cm for 15 MV x-rays. The average transmission and leakage for 6 MV and 15 MV x-rays were 6.6% and 7.4% respectively in single level of leaves fully closed. In case of dual level of leaves fully closed the measured transmission is approximately 0.5% for both 6 MV and 15 MV x-rays. Through the commissiong procedure we could verify the dose characteristics of mMLC and approximately evaluate the error ranges for treatment planning system.

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Studies on the Environmentally and Ecologically Stable Revegetation Measures on Rock Cut-Slopes - Effect of Hydroseeding Measures with Forest Topsoil - (암절취(岩切取) 훼손(毁損)비탈면에 대한 환경생태적(環境生態的)으로 안정(安定)된 녹화공법(綠化工法)에 관(關)한 연구(硏究) (II) - 산림표층토(山林表層土)를 이용한 종비토(種肥土)뿜어붙이기공법(工法)의 시공효과(施工效果) 분석(分析) -)

  • Woo, Bo-Myeong;Kim, Kyung-Hoon
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.383-390
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    • 1998
  • The purpose of this study was to evaluate the effect of new materials with forest topsoil for hydroseeding measures that is environmentally and ecologically stable revegetation measures on rock cut-slopes. Field hydroseeding experiment was used with a completely randomized design at highway rock cut-slopes in April, 1997. Results obtained in this study were summarized as follows: Particle size distribution of 3 mixed-soil materials that consisted of forest topsoil, decomposed granite soil, compost, sludge, and bottom ash did not show a significant difference. As appending the amount of forest topsoil, soil bulk density was increased. Soil hardness was slightly increased in early period, and then decreased with the flourishing of plants. The number of individuals increased, more than $3,000seedlings/m^2$, after 1 month, and it was decreased as time passes because of competition between the seeded species and the naturally emerged species. In addition to the seeded species, seeding plot has more than 6 species (Rubus crataegifolius, Eleusine indica, Erigeron canadensis, Lycopersicon esculentum var. cerasiforme, etc.) per $m^2$ were naturally appeared in the first year. From the viewpoint of species diversity promotion, the capability of using forest topsoil as seed bank sources was high. In order to apply in the field, the investigation and analysis of topsoil availability (quantity and quality of seed source, soil texture and organic composition) should be carried out before-hand.

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Cloning and Phylogenetic Characterization of Coat Protein Genes of Two Isolates of Apple mosaic virus from ¡?Fuji¡? Apple

  • Lee, Gung-Pyo;Ryu, Ki-Hyun;Kim, Hyun-Ran;Kim, Chung-Sun;Lee, Dong-Woo;Kim, Jeong-Soo;Park, Min-Hye;Noh, Young-Mi;Choi, Sun-Hee;Han, Dong-Hyun;Lee, Chang-Hoo
    • The Plant Pathology Journal
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    • v.18 no.5
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    • pp.259-265
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    • 2002
  • Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was detected and isolated from diseased 'Fuji' apple (Malus domestica) in Korea. The coat protein (CP) genes of two ApMV strains, denoted as ApMV-Kl and ApMV-K2, were amplified by using the reverse transcription and polymerase chain reaction (RT-PCR) and were analyzed thereafter. The objectives were to define the molecular variability of genomic information of ApMV found in Korea and to develop virus-derived resistant gene source for making virus-resistant trans-genic apple. RT-PCR amplicons for the APMVS were cloned and their nucleotide sequences were determined. The CPs of ApMV-Kl and ApMV-K2 consisted of 222 and 232 amino acid residues, respectively. The identities of the CPs of the two Korean APMVS were 93.1% and 85.6% at the nucleotide and amino acid sequences, respectively. The CP of ApMV-Kl showed 46.1-100% and 43.2-100% identities to eight different ApMV strains at the nucleotide and amino acid levels, respectively. When ApMV-PV32 strain was not included in the analysis, ApMV strains shared over 83.0% and 78.6% homologies at the nucleotide and amino acid levels, respectively. ApMV strains showed heterogeneity in CP size and sequence variability. Most of the amino acid residue differences were located at the N-termini of the strains of ApMV, whereas, the middle regions and C-termini were remarkably conserved. The APMVS were 17.(1-54.5% identical with three other species of the genus Ilarviyus. ApMV strains can be classified into three subgroups (subgroups I, II, and III) based on the phylogenetic analysis of CP gene in both nucleotide and amino acid levels. Interestingly, all the strains of subgroup I were isolated from apple plants, while the strains of subgroups II and III were originated from peach, hop, or pear, The results suggest that ApMV strains co-evolved with their host plants, which may have resulted in the CP heterogeneity.

Development and Application of Detection Method for Aphanizomenon flos-aquae not Usable as a Food Materials in Korea (식품 중 사용금지 원료인 Aphanizomenon flos-aquae 검출법 개발 및 응용)

  • Park, Yong-Chjun;Shin, Seung-Jung;Lee, Ho-Yeon;Kim, Yong-Sang;Kim, Mi-Ra;Lee, Sang-Jae;Lee, Hwa-Jung
    • Journal of Food Hygiene and Safety
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    • v.28 no.2
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    • pp.188-193
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    • 2013
  • Anatoxin-a, saxitoxin and neosaxitoxin are produced by Aphanizomenon flos-aquae that is a sort of the cyanobacteria phylum. Therefore, it is not permitted for food materials in Korea. Traditionally, the classification of cyanobacteria has been based on morphological characters such as trichome width, cell size, division planes, shape, and the presence of character such as gas vacuole. But, some diagnostic features, such as gas vacuole or akinetes, can show variation with different environmental or growth conditions and even be lost during cultivation. Therefore, we developed detection method for functional foods containing Aph. flos-aquae by PCR. To design the primer, 16S rRNA region of Aph. flos-aquae, Spirulina laxissima, and Spirulina spp. registered in the GeneBank (www.ncbi.nlm.nih.gov) have been used and for comparative analysis, BioEdit ver. 7.0.9.0. was used. As a result, we was design AFA-F1/AFA-R1 (363 bp) primer for the differentiation Aph. flos-aquae from chlorella, spirulina, green tea, and spinach. Also, it could be distinguished chlorella and spirulina products those are made to contain 1% Aph. flos-aquae.