• Journal of Ginseng Research
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• 제45권1호
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• pp.48-57
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• 2021

Background: Ginsenoside Rh2 is well known for many pharmacological activities, such as anticancer, antidiabetes, antiinflammatory, and antiobesity properties. Glycosyltransferases (GTs) are ubiquitous enzymes present in nature and are widely used for the synthesis of oligosaccharides, polysaccharides, glycoconjugates, and novel derivatives. We aimed to synthesize new ginsenosides from Rh2 using the recombinant GT enzyme and investigate its cytotoxicity with diverse cell lines. Methods: We have used a GT gene with 1,224-bp gene sequence cloned from Lactobacillus rhamnosus (LRGT) and then expressed in Escherichia coli BL21 (DE3). The recombinant GT protein was purified and demonstrated to transform Rh2 into two novel ginsenosides, and they were characterized by nuclear magnetic resonance (NMR) techniques and evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide assay. Results: Two novel ginsenosides with an additional glucopyranosyl (6→1) and two additional glucopyranosyl (6→1) linked with the C-3 position of the substrate Rh2 were synthesized, respectively. Cell viability assay in the lung cancer (A549) cell line showed that glucosyl ginsenoside Rh2 inhibited cell viability more potently than ginsenoside Rg3 and Rh2 at a concentration of 10 μM. Furthermore, glucosyl ginsenoside Rh2 did not exhibit any cytotoxic effect in murine macrophage cells (RAW264.7), mouse embryo fibroblasts cells (3T3-L1), and skin cells (B16BL6) at a concentration of 10 μM compared with ginsenoside Rh2 and Rg3. Conclusion: This is the first report on the synthesis of two novel ginsenosides, namely, glucosyl ginsenoside Rh2 and diglucosyl ginsenoside Rh2 from Rh2 by using recombinant GT isolated from L. rhamnosus. Moreover, diglucosyl ginsenoside Rh2 might be a new candidate for treatment of inflammation, obesity, and skin whiting, and especially for anticancer.

• 수산경영론집
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• 제42권1호
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• pp.85-96
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• 2011

We have done the input-output analysis to see the over all impact of flounder industry of Jeju region on the domestic economy of Korea. To do the input-output analysis, we have constructed the data set for the input-output table by using the existing data set in the "2003 input- output table of Jeju regional area" published by the joint work of Jeju branch of Korea bank and the Jeju Development Institute, together with some raw data provided by Jejudo Marine Fish-Culture Cooperative. We have also produced input coefficient of flounder industry by making flounder industrial sector exogenous, separated from intermediate demand. To summarize our empirical results, the inducement effect of production, value added, and employment of Jeju flounder aquaculture industry are 300 billion won, 116 billion won and 1,800 people respectively. In conclusion, the results of this study suggest flounder industry of Jeju region contributes powerfully to not only Jeju economy but also all over the Korea economy.

• IMMUNE NETWORK
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• 제4권2호
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• pp.88-93
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• 2004

Background: Bone marrow mesenchymal stem cells (MSC) inhibit the immune response of lymphocytes to specific antigens and dendritic cells (DC) are professional antigenpresenting cells whose function is to present antigen to naive T-lymphocytes with high efficiency and play a central role in the regulation of immune response. We studied the effects of MSC on DC to evaluate the relationship between MSC and DC in transplantation immunology. Methods: MSC were expanded from the bone marrow and DC were cultured from peripheral blood mononuclear cells (PBMNC) of 6 myelogenous leukemia after achieving complete response. Responder cells isolated from PBMNC and lysates of autologous leukemic cells are used as tumor antigen. The effect of MSC on the DC was analyzed by immunophenotype properties of DC and by proliferative capacity and the amount of cytokine production with activated PBMNC against the allogeneic lymphocytes. Also, cytotoxicity tests against leukemic cells studied to evaluate the immunologic effect of MSC on the DC. Results: MSC inhibit the CD83 and HLA-class II molecules of antigen-loaded DC. The proliferative capacity and the amount of INF-$\gamma$ production of lymphocytes to allogeneic lymphocytes were decreased in DC co-cultured with MSC. Also the cytotoxic activity of lymphocytes against leukemic cells was decreased in DC co-cultured with MSC. Conclusion: MSC inhibit the activation and immune response of DC induced by allogeneic or tumor antigen.

• 문화기술의 융합
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• 제8권4호
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• pp.469-476
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• 2022

3D 모델링 기술과 대화 인공지능 알고리즘의 발달로 인해 다양한 분야에서 디지털 휴먼이 활용되고 있고, 신한 은행, 농협 은행 등 주요 은행을 중심으로 은행에서도 가상 은행원이 등장하기 시작했다. 그러나 디지털 휴먼과 관련된 연구는 주로 외형 위주이며, 로봇 의인화 과정에서 고려되어야 하는 퍼소나 설정에 대한 연구는 미비하다. 본 연구에서는 은행 업무라는 구체적인 맥락에서 발화 전략의 의인화 수준과 개인정보 활용 수준이 다른 3가지 시나리오(학생증 수령, 입출금 통장 개설, 전세 자금 대출 상담)에 대한 사용자 경험을 알아보기 위해 실험을 진행하였다. 연구 결과, 사회적 실재감과 유용성은 시나리오와 의인화 수준에 대해 상호작용 효과가 있었다. 친밀감, 신뢰도, 인지된 지능에는 상호작용 효과가 없었지만 경향성을 확인 할 수 있었다.

• 경제분석
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• 제27권4호
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• pp.43-69
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• 2021

한국은 중국과의 교역 규모가 큰 데다 금융부문의 연계성 또한 점차 강화되고 있어 중국 통화정책 변화가 다양한 경로를 통해 한국 경제에 파급영향을 미치고 있다. 이에 본 연구는 중국 통화정책 운영상의 특징과 최근 운영 현황을 살펴보고, VAR 모형을 이용하여 중국 통화정책 변화가 한국 경제에 미치는 영향과 파급경로에 대해 분석하였다. 중국 통화정책 변화의 파급경로를 대중 수출 및 무역수지 등 한국 교역변수(무역경로)와 이자율, 주가, 물가 등 금융변수(금융경로, 원유가격경로)에 미치는 영향으로 나누어 분석한 결과, 중국 통화정책 완화 충격은 무역경로 중 수직적 무역통합 경로를 통해 한국의 대중 중간재 수출을 증가시킨 것으로 나타났다. 반면 지출전환 경로와 소득수요 경로는 유의하지 않은 것으로 나타났다. 금융경로 및 원유가격경로에서는 글로벌 투자자금 흐름 재조정, 금리차 및 원자재가격 변동 등을 통해 중국 통화정책 완화 충격이 한국 금리 하락과 주가 및 물가 상승을 유발하는 것으로 나타났다.

• 무역학회지
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• 제41권4호
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• pp.41-59
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• 2016

한 국가의 기업가정신이 경제성장에 기여하는 영향요인으로 주목받고 있는 상황이다. 본 연구는 기업가정신을 기업가적 행동과 환경으로 세분화하여 이들이 국가의 경제성장에 미치는 영향에 대하여 분석하고자 하였다. 기존 연구에서는 기업가정신이라는 변수를 중심으로 경제성장과의 관계성을 분석하였다면, 본 연구에서는 기업가적 활동과 기업가적 환경이라는 변수를 이용하여 극가들의 경제성장에 미치는 영향을 설명하고자 하였다. 더 나아가, 무역과 혁신이 제시된 독렵변수들과 경제성과와의 관계에서 조절적 역할을 수행하는 지를 검증하고자 하였다. GEM의 Adult Population Survey(APS)와 National Expert Survey(NES), World bank에서 데이터를 수집하여 패널분석을 실시한 결과, 기업가적 행동과 기업가 환경요인은 모두 혹은 부분적으로 국가별 경제성장에 유의한 정의 영향을 미치는 것으로 분석되었다. 특히, 기회추구형 창업활동이 경제성장에 정의 영향을 주는 것으로 나타나 창업활동의 질적인 측면이 경제성장의 성과로 이어지는데 중요한 변수임을 도출하였다. 또한, 정부 창업 지원프로그램과 경제성장의 관계에 대하여 무역과 혁신이 유의하게 조절효과를 갖는 것을 확인하였으며, 본 연구 결과에 따른 시사점에 대하여 논의하였다.

• 한국미생물·생명공학회지
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• 제36권1호
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• pp.21-27
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• 2008

고추탄저병을 생물학적으로 방제할 수 있는 미생물을 얻기 위하여 고추탄저균 C. acutatum ACYSJ001의 성장을 억제하는 점액세균을 탐색하였다. Myxococcus속에 속하는 59균주와 Corallococcus 속에 속하는 34균주 등 총 93균주의 용균성 점액세균에 대해 고추탄저균인 C. acutatum ACYSJ001에 대한 성장 억제 활성을 조사한 결과, Corollococcus속에 속하는 대부분의 점액세균들이 C. acutatum ACYSJ001의 성장을 저해하지 않는 반면, Myxococcus속으로 동정된 10균주들은 C. acutatum ACYSJ001의 성장을 강하게 저해하는 것으로 나타났다. 이러한 결과는 Myxococcus속에 속하는 여러 점액세균들이 고추탄저병을 억제하는 미생물로서 활용될 수 있는 가능성이 높으며, 또한 새로운 항진균물질 탐색을 위한 좋은 생물연구소재임을 보여주었다.

• 무역상무연구
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• 제58권
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• pp.179-213
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• 2013

While a performance type guarantee is required as a security for non-performance risk by a seller, a payment guarantee is used as a security for non-payment risk by a buyer(or a borrower in a loan agreement). A payment guarantee is a type of independent bank guarantee, bank guarantee, bond, demand guarantee, or standby letter of credit. A guarantor accepts a credit risk of a principal which is normally a buyer in a contract for sale of goods. A payment guarantee is independent of the underlying relationship between the applicant and the beneficiary. The guarantor is only empowered to examine the beneficiary's demand and determine the payment on its face to the terms of the guarantee. A payment guarantee is thus different from a suretyship. The principle of independence carries a significant advantages for a guarantor as well as for a beneficiary. While a documentary credit requires B/L, commercial invoice, packing list, inspection certificate, etc., a typical payment guarantee does not require any evidence for a seller's performance of the underlying contract other than written demand. In this respect payment guarnatee can be a more secured facility than a documentary credit. A payment guarantee normally comes into force from the issuing date and shall remain in effect until all sums guaranteed shall be paid in full by a buyer(or a borrower) or by a guarantor. Although a guarantor shall pay a demand made in accordance with the terms and conditions of the payment guarantee, a payment demand may be denied when it is determined to be abusive or unfair.

• Journal of Ginseng Research
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• 제42권2호
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• pp.123-132
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• 2018

Ginseng has gained its popularity as an adaptogen since ancient days because of its triterpenoid saponins, known as ginsenosides. These triterpenoid saponins are unique and classified as protopanaxatriol and protopanaxadiol saponins based on their glycosylation patterns. They play many protective roles in humans and are under intense research as various groups continue to study their efficacy at the molecular level in various disorders. Ginsenosides Rb1 and Rg1 are the most abundant ginsenosides present in ginseng roots, and they confer the pharmacological properties of the plant, whereas ginsenoside Rg3 is abundantly present in Korean Red Ginseng preparation, which is highly known for its anticancer effects. These ginsenosides have a unique mode of action in modulating various signaling cascades and networks in different tissues. Their effect depends on the bioavailability and the physiological status of the cell. Mostly they amplify the response by stimulating phosphotidylinositol-4,5-bisphosphate 3-kinase/protein kinase B pathway, caspase-3/caspase-9-mediated apoptotic pathway, adenosine monophosphate-activated protein kinase, and nuclear factor kappa-light-chain-enhancer of activated B cells signaling. Furthermore, they trigger receptors such as estrogen receptor, glucocorticoid receptor, and N-methyl-$\text\tiny{D}$-aspartate receptor. This review critically evaluates the signaling pathways attenuated by ginsenosides Rb1, Rg1, and Rg3 in various tissues with emphasis on cancer, diabetes, cardiovascular diseases, and neurodegenerative disorders.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제27권6호
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• pp.523-527
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• 2005

Allograft donations are commonly found to be contaminated. The most of tissue banks has promoted the use of ionizing radiation for the sterilization of biological tissues. The potential for transmission of human infectious diseases and contamination of microorganism has created serious concern for the continued clinical use of hard and soft-tissue allografts. Tissue banks have employed 15-25kGy for sterilization of hard and tendon allografts, which, according to the national standards, approaches the level at which the tissue quality is adversely affected for transplantation. The donations of allogeneic tissues to the Korea Tissue Bank over a 2-year period were reviewed, and the incidence and bacteriology of contamination were detailed. Clinical outcomes were determined for donors who had positive cultures at the time of retrieval and during the processing and they were compared with those of post sterilization. After exposure of the frozen block bone to 25kGy and the processed tissues to 15kGy of gamma irradiation, the authors were able to demonstrate complete inactivation of the bacteria. The aim of this study was to obtain the effects of gamma irradiation and the irradiation dose according to the type of tissue, through conventional microbiologic test without on influence of biocompatibility in allografts. The contamination rate after the final irradiation sterilization is 0% in the processed allografts. This may be due to the fact that the gamma radiation and processing steps are effective to control contamination.