• Journal of Korean Medicine Rehabilitation
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• v.24 no.2
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• pp.15-29
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• 2014

Objectives We have studied to know effects of Bangkeehwangkee-tang on the monosodium iodoacetate (MIA) induced osteoarthritis in rats. Methods Osteoarthritis was induced by injection of monosodium iodoacetate into knee joint cavity of rats. Rats are devided into 4 groups; Sham, control, Bangkeehwangkee-tang, Shinbaro. Sham group was injected by normal saline into knee joint cavity only. Control group was taken no treatment and Experimental groups were taken extracts of Bangkeehwangkee-tang and Shinbaro by orally once a day for 4 weeks. The content of TNF-$\alpha$, IL- $1{\beta}$ and IL-6 in synovial fluids were analysed. COX-2 in chondrocytes and blood PGE2 concentration were analysed. Histopathological examination was performed by Safranin-O fast green staining and Mankins score checking. Results The content of TNF-$\alpha$, IL-$1{\beta}$ in experimental groups were decreased compared with control group. COX-2 in chondrocytes and blood PGE2 concentration in experimental groups were decreased compared with control group. Histopathologically, osteoarthritic scores of experimental groups were decreased compared with control group. But the content of IL-6 in synovial fluids was not significantly compared with control group. Conclusions According to these results, it can be suggested that Bangkeehwangkee-tang has anti-arthritic effects on the MIA induced osteoarthritis in rats.

• The Journal of Pediatrics of Korean Medicine
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• v.29 no.1
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• pp.44-49
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• 2015

Objectives Hydrocele is a common disease found in pediatrics and is usually treated by hydrocelectomy, and even after the unilateral repair of hydrocele, it has been reported that the contralateral hydrocele still occurs in 5.4-12.5%. Therefore, the purpose of this study is to conduct a case study of contralateral hydrocele treated by Oryeong-san and Bangkeehwangkee-tang (五苓散 合 防己黃芪湯). Methods A patient diagnosed with San Syndrome (Su-San) (水疝) was administered Oryeong-san and Bangkeehwangkee-tang (五苓散 合 防己黃芪湯). Results The sizes of hydrocele and symptoms were reduced. Conclusions In the case of contralateral occurrence after unilateral repair of hydrocele in children, it is better to select a safer treatment than operation considering other possible complications. In this report, we have proven that Oryeong-san and Bangkeehwangkee-tang (五苓散 合 防己黃芪湯) can be very effective in reducing the size of hydrocele, improving symptoms and preventing recurrence.

• The Korea Journal of Herbology
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• v.29 no.6
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• pp.95-102
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• 2014

Objectives : The purpose of this study is to prove the effect of GamiBangkeehwangkee-tang (f$\acute{a}$ngj$\check{i}$hu$\acute{a}$ngq$\acute{i}$-t$\bar{a}$ng, BHT) ethanol extract on the immunity and rheumatoid arthritis related inflammatory cytokines. Methods : We checked viability in RAW 264.7 cell after treat by BHT. Then we measured inflammatory and immunity factors of DBA/1 mice with rheumatoid arthritis induced by collagen after BHT oral administration. Also, we checked micro-CT image, bone volume and bone inflammation ratio in micro-CT and structural parameter test. Results : BHT showed cell viability of 95% or higher in all concentration in RAW 264.7 cells. BHT treated group decreased level in serum of IgM and IgG test by 36% and 25% respectively. And BHT treated group showed significant decrease in WBC, neutrophil, lymphocyte and monocytes immune cell ratio in blood by 47%, 22%, 56% and 85% respectively. Also, BHT treated group decreased level in serum of $IL-1{\beta}$, IL-6, IL-17, $TNF-{\alpha}$ and hs-CRP tests by 29%, 33%, 32%, 24% and 56% respectively. Finally, BHT treated group showed increase ratio of bone volume that decrease ratio of bone inflammation. Conclusions : In this study, the results were observed rheumatoid arthritis factors cytokine decrease in serum. And BHT showed immunoglobulin and immune cells ratio decrease in serum and blood. Also, BHT depending on effects of inflammatory and immunity in hs-CRP test, micro-CT, structural parameter test. Thus, these results can used as a effective drug of BHT for inflammation and immunity.

• The Korea Journal of Herbology
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• v.20 no.2
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• pp.149-157
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• 2005

Objectives : Each 2 types of Bangkeehwangkeetang(BHT) and Bangkeebok-ryeongtang(BBT) was prescribed to examined the therapeutic effects on hyperlipidemia. BHT-1 and BBT-1 were composed of Bunbangkee(粉防己, Stephaniae Radix) for Bangkee with other drugs, but BHT-2 and BBT-2 were composed of Cheongpungdeung(淸風藤, Sinomenii acuti Lignum). Methods : Four decoctions prepared from the prescriptions were respectively administrated to animal models in rats such as hyperlipidemic model induced by high cholesterol diet. Results : On the hyperlipidemic model, BHT-2 and BBT-2 would decrease the levels of total cholesterol(Tc) and triglyceride(TG) in blood, on the other hand, BHT-1 and BBT-1, despite little change of Tc, decrease TG but also HDL-cholesterol(HDLc). Conclusion : BBT, especially composed of Cheongpungdeung(Sinomenii acuti Lignum) for Bangkee, could be used more effectively than the others(Stephaniae Radix) on hyperlipidemia.

• The Journal of Korean Medicine
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• v.43 no.1
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• pp.33-41
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• 2022

Objectives: Drug-induced blockade of the human ether-à-go-go related gene (hERG) potassium ion channel causes acquired long QT syndrome, which is known to cause cardiac arrhythmias and be fatal. To establish safety evidence of herbal formulae, we evaluated the effects of 31 herbal formulae on hERG channel activity. Methods: The current through hERG channel was measured by changing the membrane voltage before and after treatment with 31 herbal formulae in HEK 293 cell overexpressing hERG channel using a whole-cell patch clamp system. The current-voltage curves and the activity curves were fitted, and the hERG activity and 50% inhibitory concentration (IC₅₀) according to each herbal formula were calculated. Results: Chokyungjongok-tang, Oncheong-eum, and Cheongsangbangpung-tang strongly inhibited the hERG activity, with IC₅₀ values of 67.67, 141.2, and 296.3 ㎍/mL, respectively. Yeonkyopaedok-san, Eunkyo-san, Ukgan-san gajinphibanha, Daegunjoong-tang (except Oryzae gluten), Insamyangyoung-tang, Banhahubak-tang, SokyungHwalhyul-tang, Jodeung-san, Hyeonggaeyeongyo-tang, and Bangkeehwangkee-tang weakly inhibited hERG activity, with IC₅₀ values ranging from 400 to 1000 ㎍/mL. The other 18 herbal formulae showed very weak hERG activity inhibition of less than 50% at the highest concentration (1000 ㎍/mL). Conclusion: This study provided safety information on cardiotoxicity by cardiac arrhythmia risk assessment of herbal formulae, and is expected to be a reference data for predicting the safety and risk of herbal formulae.