• The Korean Journal of Physiology and Pharmacology
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• 제23권6호
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• pp.529-537
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• 2019

Lung cancer is the most common cause of cancer deaths worldwide and several molecular signatures have been developed to predict survival in lung cancer. Increasing evidence suggests that proliferation and migration to promote tumor growth are associated with dysregulated ion channel expression. In this study, by analyzing high-throughput gene expression data, we identify the differentially expressed $K^+$ channel genes in lung cancer. In total, we prioritize ten dysregulated $K^+$ channel genes (5 up-regulated and 5 down-regulated genes, which were designated as K-10) in lung tumor tissue compared with normal tissue. A risk scoring system combined with the K-10 signature accurately predicts clinical outcome in lung cancer, which is independent of standard clinical and pathological prognostic factors including patient age, lymph node involvement, tumor size, and tumor grade. We further indicate that the K-10 potentially predicts clinical outcome in breast and colon cancers. Molecular signature discovered through $K^+$ gene expression profiling may serve as a novel biomarker to assess the risk in lung cancer.

• Journal of Veterinary Science
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• 제22권4호
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• pp.56.1-56.10
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• 2021

Background: Fluorescent antibody virus neutralization (FAVN) test is a standard assay for quantifying rabies virus-neutralizing antibody (VNA) in serum. However, a safer rabies virus (RABV) should be used in the FAVN assay. There is a need for a new method that is economical and time-saving by eliminating the immunostaining step. Objectives: We aimed to improve the traditional FAVN method by rescuing and characterizing a new recombinant RABV expressing green fluorescent protein (GFP). Methods: A new recombinant RABV expressing GFP designated as ERAGS-GFP was rescued using a reverse genetic system. Immuno-fluorescence assay, peroxidase-linked assay, electron microscopy and reverse transcription polymerase chain reaction were performed to confirm the recombinant ERAGS-GFP virus as a RABV expressing the GFP gene. The safety of ERAGS-GFP was evaluated in 4-week-old mice. The rabies VNA titers were measured and compared with conventional FAVN and FAVN-GFP tests using VERO cells. Results: The virus propagated in VERO cells was confirmed as RABV expressing GFP. The ERAGS-GFP showed the highest titer (10^8.0 TCID₅₀/mL) in VERO cells at 5 days post-inoculation, and GFP expression persisted until passage 30. The body weight of 4-week-old mice inoculated intracranially with ERAGS-GFP continued to increase and the survival rate was 100%. In 62 dog sera, the FAVN-GFP result was significantly correlated with that of conventional FAVN (r = 0.95). Conclusions: We constructed ERAGS-GFP, which could replace the challenge virus standard-11 strain used in FAVN test.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• 제14권5호
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• pp.413-420
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• 2021

This study is about a machine learning model for predicting insufficient parameters through other parameters when one of the collected parameters is insufficient. A regression model was created to predict time, temperature, humidity, CO2, and light quantity data through the machine learning regression analysis function in Matlab. In addition, the three models with the lowest RMSE values for each parameter were selected and verified. For verification, the predicted values were obtained by applying the test data to the prediction model derived from each parameter, and the correlation coefficient and error average between the measured values and the obtained predicted values were obtained and then compared.

• The Journal of the Korea Contents Association
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• 제21권12호
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• pp.1-12
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• 2021

An objective expert discrimination scheme is needed for finding researchers who have insight and knowledge about a particular field of research. There are two types of expert discrimination schemes such as a citation graph based method and a formula based method. In this paper, we propose an efficient expert discrimination scheme considering various characteristics that have not been considered in the existing formula based methods. In order to discriminate the expertise of researchers, we present six expertise indices such as quality, productivity, contributiveness, recentness, accuracy, and durability. We also consider the number of social citations to apply the characteristics of academic search sites. Finally, we conduct various experiments to prove the validity and feasibility of the proposed scheme.

• Journal of Mycology and Infection
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• 제23권4호
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• pp.99-104
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• 2018

Background: Trichophyton verrucosum (T. verrucosum) is a zoophilic dermatophyte that causes ringworm in cattle and is prevalent worldwide. This dermatophyte may be responsible for various conditions, especially inflammatory skin lesions. Objective: In this study, we aimed to investigate the changes in the clinical and epidemiological characteristics of T. verrucosum infections in southeastern Korea. Methods: A total of 34 patients who visited the Catholic Skin Clinic and the Kyungpook National University Hospital in Daegu, Korea from 2005 to 2017 were diagnosed with T. verrucosum infection. The diagnosis was confirmed using fungal culture. The data were based on a retrospective survey of the medical records. Results: The annual incidence of T. verrucosum infection was very low. There was no difference in the sexual incidence. T. verrucosum infection was most common among subjects in their fifties and sixties. The highest incidence was during the month of September. The arm was most frequently involved, followed by the face. This infection was predominant in patients living in rural areas. Conclusion: These clinicoepidemiological findings provide useful information for understanding the changes in the infection caused by T. verrucosum. In particular, it was interesting to note that the incidence was very low, majority of the affected subjects were in their fifties and sixties, and the most commonly affected site was the arm.

• Journal of Ginseng Research
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• 제43권1호
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• pp.86-94
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• 2019

Background: Ginseng is believed to have antitumor activity. Autophagy is largely a prosurvival cellular process that is activated in response to cellular stressors, including cytotoxic chemotherapy; therefore, agents that inhibit autophagy can be used as chemosensitizers in cancer treatment. We examined the ability of Korean Red Ginseng extract (RGE) to prevent autophagic flux and to make hepatocellular carcinoma (HCC) cells become more sensitive to doxorubicin. Methods: The cytotoxic effects of total RGE or its saponin fraction (RGS) on HCC cells were examined by the lactate dehydrogenase assay in a dose- or time-dependent manner. The effect of RGE or RGS on autophagy was measured by analyzing microtubule-associated protein 1A/1B-light chain (LC)3-II expression and LC3 puncta formation in HCC cells. Late-stage autophagy suppression was tested using tandem-labeled green fluorescent protein (GFP)-monomeric red fluorescent protein (mRFP)-LC3. Results: RGE markedly increased the amount of LC3-II, but green and red puncta in tandem-labeled GFP-mRFP-LC3 remained colocalized over time, indicating that RGE inhibited autophagy at a late stage. Suppression of autophagy through knockdown of key ATG genes increased doxorubicin-induced cell death, suggesting that autophagy induced by doxorubicin has a protective function in HCC. Finally, RGE and RGS markedly sensitized HCC cells, (but not normal liver cells), to doxorubicin-induced cell death. Conclusion: Our data suggest that inhibition of late-stage autophagic flux by RGE is important for its potentiation of doxorubicin-induced cancer cell death. Therapy combining RGE with doxorubicin could serve as an effective strategy in the treatment of HCC.

• Journal of Korean Society of Industrial and Systems Engineering
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• 제42권1호
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• pp.33-40
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• 2019

This study focuses on a job-shop scheduling problem with the objective of minimizing total tardiness for the job orders that have different due dates and different process flows. We suggest the dispatching rule based scheduling algorithm to generate fast and efficient schedule. First, we show the delay schedule can be optimal for total tardiness measure in some cases. Based on this observation, we expand search space for selecting the job operation to explore the delay schedules. That means, not only all job operations waiting for process but also job operations not arrived at the machine yet are considered to be scheduled when a machine is available and it is need decision for the next operation to be processed. Assuming each job operation is assigned to the available machine, the expected total tardiness is estimated, and the job operation with the minimum expected total tardiness is selected to be processed in the machine. If this job is being processed in the other machine, then machine should wait until the job arrives at the machine. Simulation experiments are carried out to test the suggested algorithm and compare with the results of other well-known dispatching rules such as EDD, ATC and COVERT, etc. Results show that the proposed algorithm, MET, works better in terms of total tardiness of orders than existing rules without increasing the number of tardy jobs.

• The Korean Journal of Physiology and Pharmacology
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• 제23권2호
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• pp.141-150
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• 2019

Despite increased evidence of bio-activity following far-infrared (FIR) radiation, susceptibility of cell signaling to FIR radiation-induced homeostasis is poorly understood. To observe the effects of FIR radiation, FIR-radiated materials-coated fabric was put on experimental rats or applied to L6 cells, and microarray analysis, quantitative real-time polymerase chain reaction, and wound healing assays were performed. Microarray analysis revealed that messenger RNA expressions of rat muscle were stimulated by FIR radiation in a dose-dependent manner in amount of 10% and 30% materials-coated. In 30% group, 1,473 differentially expressed genes were identified (fold change [FC] > 1.5), and 218 genes were significantly regulated (FC > 1.5 and p < 0.05). Microarray analysis showed that extracellular matrix (ECM)-receptor interaction, focal adhesion, and cell migration-related pathways were significantly stimulated in rat muscle. ECM and platelet-derived growth factor (PDGF)-mediated cell migration-related genes were increased. And, results showed that the relative gene expression of actin beta was increased. FIR radiation also stimulated actin subunit and actin-related genes. We observed that wound healing was certainly promoted by FIR radiation over 48 h in L6 cells. Therefore, we suggest that FIR radiation can penetrate the body and stimulate PDGF-mediated cell migration through ECM-integrin signaling in rats.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• 제29권11호
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• pp.834-841
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• 2018

A power amplifier for subgigahertz short-range wireless communication using $0.18-{\mu}m$ CMOS technology is presented. It is designed as a differential structure to form easily a virtual ground node, to increase output power, and to design a cascode structure to prevent breakdown. The transistor gate width was determined to maximize the output power and power-added efficiency(PAE), and the balun was optimized through electromagnetic simulation to minimize the loss caused by the matching network. This power amplifier had a gain of more than 49.5 dB, a saturation power of 26.7 dBm, a peak PAE of 20.7 % in the frequency range of 860 to 960 MHz, and a chip size of $2.14mm^2$.

• Fire Science and Engineering
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• 제32권6호
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• pp.91-99
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• 2018

Recently, the spread of ESS in Korea has increased and a fire accident has also occurred. By July 2018, there were a total of 7 cases. All 7 cases were ESS systems consisting of lithium-ion batteries and were burned down. Both the automatic fire extinguisher and the fire department were not able to digest. In this paper, the characteristics of ESS fire are analyzed based on recent ESS fire situation and field investigation, and the cause of fire is divided into environmental, electrical and thermal factors. As a result, it was found that the ESS fire was correlated with the installation environment of the system. In the domestic and overseas lithium ion battery test standard and ESS facility standard survey, the trends and differences of domestic and overseas facilities standards were identified. Based on the fire status and field investigationy, and domestic and overseas facility standard survey, measures were suggested to prevent and prevent the spread of fire in ESS fire.