• Journal of Veterinary Science
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• v.21 no.5
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• pp.64.1-64.14
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• 2020

Background: Canine distemper virus (CDV) infection results in high morbidity and mortality in dogs. There has been no report about Isolation of Korean CDV since 1980 in Korea. Objectives: To investigate the biological properties and the genetic characterization of Korean CDV. Methods: Vero cells expressing dog signaling lymphocyte activation molecule (dSLAM) gene named as Vero/dSLAM were used to isolate CDV using 17 samples. Diagnostic methods such as cytopathic effects, immunofluorescence assay, peroxidase linked assay, electron microscopy, rapid immunodiagnostic assay, and reverse transcription polymerase chain reaction were used to confirm the Korean CDV isolate as a CDV. The genetic analysis was performed through cloning and sequencing of hemagglutinin gene of CDV isolate. Results: A virus propagated in Vero/dSLAM cell was confirmed as CDV (CD1901 strain) based on the above methods. The CD1901 strain showed the highest viral titer (10^5.5 50% tissue culture infectious dose [TCID₅₀]/mL) in the Vero/dSLAM cells at 4 days post inoculation, but did not form a fork on chorioallantoic membrane of 7-day-old egg. Ribavirin, a nucleotide analogue anti-viral agent, inhibits moderately the Korean CDV propagation in the Vero/dSLAM cells. The nucleotide and amino acid sequences of the H gene of CD1901 strain were compared with those of other CDV strains. The CD1901 strain belonged to Asia 1 group and had the highest similarity (99.9%) with the BA134 strain, which was isolated in China in 2008. Conclusions: We constructed successfully Vero/dSLAM and isolated one Korean CDV isolate (CD1901 strain) from a naturally infected dog. The CD1901 strain belonged to Asia 1 genotype.

• Journal of The Korean Society of Integrative Medicine
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• v.10 no.3
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• pp.199-208
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• 2022

Purpose : The study aimed to examine gender-differences in muscle tone, elasticity, and stiffness of the erector spinae in young and old subjects. This study also aimed to assess the effect of aging on muscle tone, elasticity, and stiffness of the erector spinae in men and women, and compare the trend of the aging effect between the two gender groups. Methods : With the muscle in the relaxed state and subjects in the prone position, a myotonometer was used to quantify muscle tone, elasticity, and stiffness, of the erector spinae in 102 participants[46 males (29 young subjects, aged 22.48±2.23 years and 17 old subjects, aged 76.35±3.71 years), 56 females (40 young subjects, aged 20.38±1.43 years and 16 old subjects, aged 74.56±5.40 years)]. Results : The tone and stiffness of the erector spinae muscles were greater in men than in women for both age groups (p<.001-.01), while elasticity did not show a significant difference between men and women. For the direction of change, both male and female groups showed significantly increased tone and stiffness, and decreased elasticity with increasing age (p<.001). For age-related changes, a different tendency was observed between men and women. Men showed a greater increase in tone than women with aging. in contrast, both men and women exhibited a similar decrease or increase in elasticity and stiffness. Conclusion : Gender-differences in the erector spinae in terms of muscle tone and stiffness were observed. Regardless of the age, men had higher muscle tone and stiffness than women, but not elasticity. The erector spinae muscles showed age-related changes in all aspects of muscle tone, elasticity, and stiffness, in both men and women. Notably, men presented greater variation than women in the amount of increase of muscle tone with aging. These findings have implications for musculoskeletal therapeutic approaches, and gender-customized tuning may be indicated for designing exercise interventions to prevent and manage gender-sensitive muscular injuries or diseases and frailty.

• Korean journal of applied entomology
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• v.61 no.3
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• pp.449-460
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• 2022

The morphology and whole genome sequence of Spodoptera exigua nucleopolyhedrovirus K1 (SeNPV-K1) isolated in Korea were analyzed for the use as an eco-friendly control source against S. exigua. The polyhedra of SeNPV-K1 was amorphous with a size of 0.6-1.8 ㎛, and there was no external difference with the previously reported SeNPV. As a result of analyzing the nucleotide sequence of the whole genome, it was composed of 135,756 bp, which is 145 bp more than that of the previously reported SeNPV. The G+CG+C content was 44% and there were 6 homologous repeated sequences, so there was no significant difference from the previous report. As a result of ORF analysis, SeNPV-K1 had 137, two fewer than those previously reported, and 4 ORFs present only in SeNPV-K1 were confirmed. These 4 ORFs are non-essential genes and were not considered to have a significant influence on the characteristics of the SeNPV. The genome vista analysis showed that the overall sequence similarity between SeNPV-K1 and the previously reported SeNPV was very high. The whole genome of SeNPV-K1 analyzed for the first time in Korea was found to be similar to the previously reported SeNPV, but it was confirmed that it was a novel resource in Korea with different isolate.

• Proceedings of the Korea Water Resources Association Conference
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• 2022.05a
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• pp.478-478
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• 2022

섬진강은 하굿둑이 없는 열린 하구로서 하구로부터 약 21km까지 조석의 영향을 받아 강물의 염도가 시간에 따라 변하는 환경이다. 오랫동안 섬진강 하구는 다양한 원인으로부터 바다화로 대표되는 염하구 문제가 지역 현안 사항으로 제기되어 왔다. 상류에서의 용수사용 증가로 인한 하천 유하량 감소 또한 그 원인들 중 하나로 판단됨에 따라 실제 하구까지 내려오는 하천유량과 바다로부터 유입되는 해수를 구분하여 정량화하는 연구가 필요한 사안이다. 본 연구의 목적은 섬진강 수계 하구에서의 다양한 생태환경을 보전하기 위한 적정 염분유지가 요구됨에 따라 섬진강하구 염분계측기(섬진강대교)를 설치하여 염분농도를 관측하고 하천유량, 하천취수 및 해양조위에 따른 염분농도 변화를 모의하여 하천유량과 염분과의 관계를 제시하고자 하였다. 본 연구에서는 EFDC(Environmental Fluid Dynamics Code) 수치모델을 이용하여 상류로는 구례군(송정리) 수위관측소부터 하류로는 여수해만 및 문의리까지의 구역을 설정하고 광양조위, 하동수위 및 고정식 염분 계측기 관측염분농도 자료를 이용하여 수치모델링의 재현성을 검증하였다. 검증 결과, 결정계수(R²)는 0.87(하동수위), 0.93(광양조위), 0.56(섬진강대교 염도)를 나타냈다. 모델 검보정 후 하천유량에 따른 염분변화 실험을 실시하여 염분농도 거동을 분석하였다. 모델 결과, 섬진강하구에서의 염분거동은 소조기때 염분체류 현상으로 인해 대조기 거동과는 큰 차이를 나타냈다. 따라서, 모델링 결과를 이용한 유량-염분 조견표는 각각 대조기와 소조기로 구분하여 산정하였다. 대조기때는 송정유량이 10톤/초의 평균갈수량이 흐를 경우 다압에서의 취수량이 2.52톤/초 ~ 4.63/초로 증가할수록 18.8psu ~ 19.9psu로 증가하였다. 소조기의 경우는 25.5psu ~ 25.7psu로 대조기와 비교하여 크게 증가됨을 나타냈다. 본 연구의 결과는 객관적인 생태환경 보전을 위한 적정염분농도 범위가 도출된다면 이를 유지하기 위한 필요유량과 필요유량을 확보하기 위한 장단기적인 대책수립이 가능할 것으로 기대된다.

• Journal of Microbiology and Biotechnology
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• v.33 no.5
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• pp.591-599
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• 2023

Fisetin is a bioactive flavonol molecule and has been shown to have antioxidant potential, but its efficacy has not been fully validated. The aim of the present study was to investigate the protective efficacy of fisetin on C2C12 murine myoblastjdusts under hydrogen peroxide (H₂O₂)-induced oxidative damage. The results revealed that fisetin significantly weakened H₂O₂-induced cell viability inhibition and DNA damage while blocking reactive oxygen species (ROS) generation. Fisetin also significantly alleviated cell cycle arrest by H₂O₂ treatment through by reversing the upregulation of p21WAF1/CIP1 expression and the downregulation of cyclin A and B levels. In addition, fisetin significantly blocked apoptosis induced by H₂O₂ through increasing the Bcl-2/Bax ratio and attenuating mitochondrial damage, which was accompanied by inactivation of caspase-3 and suppression of poly(ADP-ribose) polymerase cleavage. Furthermore, fisetin-induced nuclear translocation and phosphorylation of Nrf2 were related to the increased expression and activation of heme oxygenase-1 (HO-1) in H₂O₂-stimulated C2C12 myoblasts. However, the protective efficacy of fisetin on H₂O₂-mediated cytotoxicity, including cell cycle arrest, apoptosis and mitochondrial dysfunction, were greatly offset when HO-1 activity was artificially inhibited. Therefore, our results indicate that fisetin as an Nrf2 activator effectively abrogated oxidative stress-mediated damage in C2C12 myoblasts.

• Journal of Dental Rehabilitation and Applied Science
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• v.38 no.4
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• pp.222-232
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• 2022

Purpose: This study was to investigate the dentists' experience and awareness of foreign body ingestion and aspiration during dental prosthetic treatment. Materials and Methods: A survey of 157 dentists working at 108 dental institutions in Daejeon and Chungcheong provinces was carried out and frequency analysis was conducted. Chi-square test was conducted to find out the difference between the sociodemographic data and experience of foreign body ingestion and aspiration of patients and independent sample t-test was conducted to find out the difference in awareness depending on whether receiving related education. Results: The percentage of dentists experiencing dropping foreign body into patients' oral cavity was 99.4% and the percentage of dentists experiencing foreign body ingestion and aspiration of patients was 53.5%. There were more dentists who experienced foreign body ingestion and aspiration of patients in male than female, longer working career, and general practitioners than specialists (P < .05). 50.3% of the respondents received related education. When they receive related education, they had low degree of concern, high confidence in coping with situations, and low willingness to receive education in the future (P < .05). Conclusion: The percentage of foreign body dropping was 16% higher in prosthetic treatment than general treatment. The percentage of dentists with experience of foreign body ingestion and aspiration of patients was 53.5%. Among them, 92.9% experienced foreign body ingestion of patients and 7.1% of them experienced foreign body aspiration of patients.

• Journal of the Korean Burn Society
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• v.19 no.1
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• pp.12-15
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• 2016

Purpose: Firefighters are vulnerable to burn injury during firefighting. In extensive fires, conducted heat and radiant heat can cause burn injury even though firefighters are not directly exposed to fire. There has been increasing interest in the health problems of firefighters considerably since Hongje-dong fire of 2001, which claimed the lives of six fireman. However, there have been no studies done on the characteristics of firefighter burn injuries in South Korea. Therefore, we investigated the characteristics of firefighter burn injuries in a burn center. Methods: A retrospective, single-center research was performed between Jan 2006 to Dec 2015. 24 firefighters came to the burn center. The electronic medical records of patients were reviewed. Results: Flame burns (87.5%) were the major cause of burn in firefighter. All the patients suffered second-degree or third-degree burns. Mean burn size was 6.1±6.7%. 22 of 24 patients were hospitalized and 2 of 22 hospitalized patients admitted to intensive care unit. Mean length of hospitalization was 29.1±23.7 days and mean length of intensive care unit hospitalization was 6.0±1.4 days. The face was the site most commonly burned, representing 25.8% of injuries. The hand/wrist, upper extremity, and neck were the next largest groups, with 19.4, 12.9, 11.3% of the injuries, respectively. Conclusion: Firefighter burn injuries occur to predictable anatomic sites with common injury patterns. The burn size was small but, admitted patients need about 30 days of hospitalization.

• Journal of Gastric Cancer
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• v.23 no.2
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• pp.365-373
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• 2023

• Journal of Digestive Cancer Research
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• v.2 no.2
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• pp.75-77
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• 2014

Ampulla of Vater (AOV) cancer is rare malignant tumor which arises within the vicinity of the AOV. Metastatic AOV adenocarcinoma has poor prognosis, with an overall survival rate at 2 years ranging from 5 to 10%. The Surveillance, Epidemiology and End Results Program of the National Cancer Institute indicated that lymph node metastasis was present in as many as half of patients which were associated with poor prognosis and liver was the second most common site of distant metastasis in AOV cancer. In this case report, we describe a case of complete resolution of AOV cancer, which was already spread to retroperitoneal lymph node and liver. The patient underwent gemcitabine plus cisplatin chemotherapy for palliative aim. After 12 month of chemotherapy, image study showed partial remission, so intraoperative radiofrequency ablation therapy and pylorus preserving pancreaticoduodenectomy was done. AOV cancer was completely resected and the patient was followed up without recurrence for 7 months.

• Korean Journal of Organic Agriculture
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• v.31 no.4
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• pp.395-412
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• 2023

The morphology and whole genome sequence of Hyphantria cunea nucleopolyhedrovirus W1 (HycuNPV-W1) isolated in Korea were analyzed for the use as an eco-friendly control agent against H. cunea. The HycuNPV-W1 had irregular tetrahedral polyhedra with a size of 1.5-2.2 ㎛ which is similar to that of previously reported HycuNPV isolated in Korea. As a result of whole viral genome analysis, HycuNPV-W1 was composed of 131,353 bp, which is 1,606 bp shorter than that of the previously reported HycuNPV. The G+C content was 45% and six of the homologous repeated regions were found, so there was no significant difference from the previous report. As a result of ORF analysis, HycuNPV-W1 contains total of 145 ORFs which is three ORFs less than the previous report, while two ORFs were exclusively found in HycuNPV-W1. The functions of these ORFs remains unclear and are not considered to have a significant influence on the characteristics of the HycuNPV. The genome vista analysis showed that the overall sequence identity between HycuNPV-W1 and the previously reported HycuNPV was very high. The whole genome of HycuNPV-W1 analyzed was found to be similar to those of the previously reported HycuNPV, however, it is supposed to be a novel resource in Korea with different isolate.