• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.82-87
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• 1994

This study was planned to identify the variety of Italian ryegrass using electrophoresis. Thirty seven varieties of Italian ryegrass were tested by starch gel electrophoresis. The specific electrophoretic zymograms of each variety were observed by Alcohol dehydrogenase(ADH) and Esterase. The results were surnrnerized as follows; 1. AU varieties displayed two band zones by ADH and Rf values were 0.63 and 0.6 (Table 2, Fig. 2). 2. There were five band type for ADH isozyme of 37 varieties classified with isozyme banding pattern. According to the isozyme band type 7, 2, 6, 18 and 4 varieties belong to group, I, II, III, IV, and V, respectively (Table 2). 3. The varieties displayed single band zone for Esterase isozyme and Rf value was 1.00 (Table 2, Fig. 4). 4. According to banding type, Esterase isozyme of 37 varieties classified into 3 groups, 22, 8 and 7 varieties belong to group, I , II, and III, respectively (Table 2).

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.2 s.308
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• pp.40-47
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• 2006

This paper proposes a multitoning method using threshold modulation of MJBNM(Modified Jointly Blue Noise Mask) for banding artifacts reduction. As banding artifacts in multitoning appear as uniform dot distributions around the intermediate output levels, such multitone output results in discontinuity and visually unpleasing patterns in smooth transition regions. Therefore, to reduce these banding artifacts, the proposed method rearranges the dot distribution by introducing pixels in the neighborhood of output levels that occurs banding artifacts. First of all principal cause of banding artifacts are analyzed using mathematical description. Based on this analytical result, a threshold modulation technique of MJBNM which takes account of chrominance error and correlation between channels is applied. The original threshold range of MJBNM is first scaled linearly sot that the minimum and maximum of the scaled range include two pixel more than adjacent two output levels that cover an input value. In an input value is inside the vicinity of any intermediate output levels produce banding artifacts, the output is set to one of neighboring output levels based on the pointwise comparison result according to threshold modulation parameter that determines the dot density and distribution. In this case, adjacent pixels are introduced at the position where the scaled threshold values are located between two output levels and the minimum and maximum threshold values. Otherwise, a conventional multitoning method is applied. As a result, the proposed method effectively decreased the appearance of banding artifacts around the intermediate output levels. To evaluate the quality of the multitone result, HVS-WRMSE according to gray level for gray ramp image and S-CIELAB color difference for color ramp image are compared with other methods.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.44 no.4 s.316
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• pp.17-25
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• 2007

Multilevel inkjet printer employs multiple ink droplets with variable dot size and/or different concentrations intended to preserve high fidelity color reproduction and the appearance of continuous tone. A variety of research efforts on multitoning techniques has progressed toward better image quality. However, banding artifacts appear due to the same dot distributions near the printable output levels. This results in discontinuity and visually unpleasing output, especially at the smooth tone transition region. In this paper, to reduce the banding artifacts, a multitoning method to arrange ink distribution by controlling the blending proportion of adjacent output pixels based on an improved threshold scaling function is proposed. Ink distributions across the banding regions are changed according to two factors of the threshold scaling function because these factors handle the blending point of adjacent output pixel. Therefore, 8 observers, subjectively investigated ink distributions around the printable output levels for a set of the improved threshold scaling function. For a threshold scaling function with the specific factor values, we can achieve smoother visual transition. In the experiment, the proposed method showed a reduction of banding artifacts in both u-ay and color image and represented better Performance of color reproduction.

• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.901-910
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• 2003

Using the G-, C-, and NOR-banding techniques, a karyotyping for Korean Native Pig was performed. Blood samples were collected from 50 male Korean Native Pigs that had been bred at the National Livestock Research Institute and then blood cells were prepared from in vitro cultures followed by karyotyping; G-, C-, and NOR-banding patterns of metaphase chromosomes were analyzed. The karyotype of Korean Native Pig is 38, XX or XY which consists of 5 pairs of submetacentric chromosomes(Group I), 2 pairs of acrocentric chromosomes with short p-arm(Group II), 5 pairs of medium metacentric chromosomes(Group III), 6 pairs of acrocentric chromosomes(Group IV) and metacentric X and Y sex chromosomes. On GTG-banding, the Korean Native Pig exhibited a typical and identical banding pattern in each homologous chromosomes. Overall chromosomal morphology and positions of typical landmarks of the Korean Native Pig were virtually identical to those of Committee for the Standardized Karyotype of the Domestic Pig(CSKDP). However, numbers of G-bands of the Korean Native Pig chromosomes were more than those of CSKDP. In chromosomes 1, 3, 5, 6, 7, 8, 13, 14, 15, 16, 17, 18 and X, the Korean Native Pig exhibited more separated bands as compared with CSKDP. In C-banding patterns, although the quantity of heterochromatin was variable in each chromosome, most of the Korean Native Pig chromosomes had heterochromatic C-bands on centromeres. However, the heterochromatic C-band was constantly observed on the whole Y chromosome. In AgNOR staining, the NORs were located at centromeres on the chromosomes 8 and 10. The number of NORs per metaphase ranged from 2 to 4 giving a mean value of 2.13. The number of NORs were distributed on all chromosome pair 10 but not on chromosome 8. The sizes of NORs were also differed between homologous chromosomes 8. Numbers of NORs of Korean Native Pig were significantly higher than those of Yorkshire. The pattern of pig NORs was polymorphic in breeds, individuals and cells, especially on chromosome 8.

• The Korean Journal of Malacology
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• v.16 no.1_2
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• pp.11-16
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• 2000

The electrophoretic banding patterns of 6-phosphogluconate dehydrogenase (PGD) in the two different chromosomal ploidy groups of Gyraulus were compared. The monomeric or dimeric banding patterns or allelic variations in a locus of PGD were observed in four diploid populations (Osan, Sohre, Kimpo and Kangwha) of G. convexiusculus occurring in Korea, whereas the isozyme banding patterns encoded by at least 3 different loci were shown in the tetraploid populations of G. (Torquis) groups collected from Michigan, the U.S.A. Of 3 different tetraploid groups, G. (T.) circumstriatus group showed 3 monomorphic isozyme banding patterns, and the other 2 populations showed some allelic variations. Such results provided good evidence to differentiate tetraploid subgenus Torquis group from the diploid Gyraulus populations.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.71-76
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• 1992

Starch gel electrophoresis was used to examine the banding pattern of Esterase isozyme in the leaf, root-nodule and seedling of four wild legume species, Trifolim repense, Glycine soja, Phaseolus nipponensis and Vigna uexillata. The number of band, enzyme activity and migrating rate of esterase isozyme varies depending on the species and tissues of legume plants. The isozyme banding pattern in the cotyledon and radicle of T. repense showed same pattern, however, the number of band were varible among the cotyledon of G. soja, P. nipponensis and V. vexzllata, respectively. Est-1 in the leaf of G. soja, V, vexillata. root-nodule of G. soja and seedling of V. vexillata expreesed the highest enzyme activity. The Est-1 showed the rapidest migrating rate among the isozymes.

• Journal of Plant Biology
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• v.21 no.1_4
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• pp.29-32
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• 1978

The karyotype of Lilium cernum has been analysed by means of C-banding technique. Most of clones observed were 2n=24 chromosomes which consist of two pairs of submetacentric and ten pairs of subtelocentric chromosomes, among which two pairs of chromosomes(B and E) showed secondary constriction in the short arm. In addition to these chromosomes a small supernumerary telocentric chromosome was seen in the eight clones. Sixtyeight bands were observed in the twentytwo chromosomes of complement and one band in the supernumerary chromosome. A pair of chromosome (L) did not show any band. The bnads on the chromosome. A pair of chromosome (L) did not show any band. The bands on the chromosomes were distributed in the centromere, secondary constriction and intercalary regions of arms. Of the twelve pairs of chromosomes ten pairs showed symmetric banding patterns in each, but two pairs (I and K) showed asymmetric banding patterns.

• Korean Journal of Poultry Science
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• v.16 no.1
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• pp.1-8
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• 1989

This experiment was conducted to improve the performance of chickens by the precise separation and analysis of chromosomes which are integrated genetic materials, and by the use of gene manipulation techniques. Following are the main results obtained. 1. When the chromosomes were separated through the leucocyte culture and analyzed by Giemsa banding techniques (especially by the method in which 20 layers of banding patterns could be found in chromosome ＃1), the normal Patterns of chromosomes ＃l-9 and sex chromosomes, and the location of constitutive heterochromatin without any gene activities in all chromosomes were discovered. 2. To utilize the primodial germ cells (PGC) as the genetic vector which is one of the most important gene manipulation techniques, PGC's from triploid were transplanted to normal host embryos. Since the donor PGC's(3n) were found in the gonads of growing host embryos gene manipulation in poultry using PGC's, seemed to be possible.

• The Korean Journal of Zoology
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• v.33 no.2
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• pp.222-230
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• 1990

The karyotypes of Korean Sciunis vulgaris coreas and Tamias sibiricus asiaticus were analyzed by conventional Giemsa staining and C-banding method. The diploid chromosome number (2n) of Sciunis vulgaris coreae 40 consisting of 6 metacentric, 8 submetacentric, 3 subtelocentric and 2 telocentric autosome pairs, submetacentric X and acrocentric or subtelocentric Y chromosome. The arm number (NF) of this species was obtained as 72, excluding the gonosomal arms. Tamias sibiricus asiaticus has a 2n of 38. The karyotype was represented by 3 metacentric, 4 submetacentric, 5 subtelocentric and 6 telocentric autosome paits and 2 sex chromosome. The X chromosome was submetacentric chromosome and the Y was the smallest chromosome with a median. The NF was 60. In S. vulgaris coreae constitutive heterochromatins were observed at the centromeres and telomeres. Constitutive heterochnomatins of T sibiricus asiaticus were primarily observed at the centromeres. These results suggested that non-Robensonian reanagenents and distribution of constitutive heterochromatin played an imporiant role in karyological differentiation of these species.

• Journal of Life Science
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• v.17 no.6 s.86
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• pp.762-765
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• 2007

A Giemsa C-banding method was used for the identification of somatic chromosomes and heterochromatic knob position in Korean indigenous waxy com (Zea mays L.). 5 inbred stocks were examined and their heterochromatic knob numbers ranged from 6 to 12. In comparison of homologous chromosomes of two stocks of YS-1 and MY-1, knob numbers, knob positions, arm ratios and relative length of chromosomes were different between the genotypes. The length of homologous chromosomes in YS-1 were generally larger than those of MY-1. The Giemsa method was proved to be useful for the identification of somatic chromosome and a C-banded diagram showing knob positions, arm ratios and relative length of chromosome could be used as a good tool to compare the characteristics of chromosomes of Korean indigenous waxy corn stocks.