• Journal of Ecology and Environment
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• 제29권4호
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• pp.389-398
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• 2006

To examine sediment characteristics and find anthropogenic effects on riverine wetland ecosystems, paleoecological study was carried out at Bam islands in Seoul. Three hundred cm deep sediment cores were retrieved and dated with the lamination analysis method until 36 cm depth (1986). Sediments were divided into three zones based on the depth profiles of physico-chemical variables: below 160 cm depth (before 1968), between 160 and 40cm depths and above 40cm depth (after 1986). Physico-chemical characteristics were very variable between 160 and 40cm depths and this indicates unstable sedimentation environment. Even though heavy metal concentrations were relatively low, Cd and As contents have increased continuously. Dry mass accumulation rates during $1968{\sim}1986\;and\;1987{\sim}2003$ were 140 and $21\;kg\;m^{-2}\;yr^{-1}$, respectively. This was related to flooding intensity and duration. Bulk density, water content, loss on ignition, N, C, C/N ratio were very similar to other river delta but Ca, Na and K contents were 2 to 4 times higher than others. Heavy metal contents except Pb were lower or similar to those in other studied marshes in Korea. Heavy metal and Mg contents were correlated with each other and this suggests that the source of heavy metals be parent rock. From $^{13}C$ dating dates of organic materials in sediment, it is suggested that organic matter originated from the watershed and flooding intensity in the watershed might be responsible for the source of sediments. This study provides reference data for the comparison of sediment characteristics at islands in river and for the management of Bam islands.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.178-178
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• 1996

Hepatitis B virus (HBV) infection is one of the serious problems in Southeast Asia including Korea because it causes chronic hepatitis, which can easily be transformed In fatal conditions such as cirrhosis and hepatoma. Even though lots of informations on structural characteristics and gene expression mechanisms have been accumulated, the mechanism for HBV-induced hepatocellular injury which is believed to be the consequences of the immunological response is not well understood. In order tn perform immunopathological studies for prevention and treatment of HBV infection, we designed transgenic mice as a disease model which can mimic HBV infection, In this study, a promoter-HBV DNA fragment for the preparation of HBV transgenic mice has been constructed. To add a proper enzyme site on 5' end of HBV gene, total HBV (subtype adr) gene was inserted into BamHI site of pBluescript SK vector and reextracted by PstI-SacI treatment A liver-specific promoter, rat ${\alpha}$ 2u globulin gene promoter, was insrted to pBluescript SK vector and reextracted by BamHI-PstI treatment, Promoter-HBV DNA was constructed by ligation of two fragments using identical PstI sites. For large scale production of promoter-HBV DNA, it was inserted to BamHI-SacI site of pBluescript SK vector.

• Food Science and Biotechnology
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• 제18권4호
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• pp.895-899
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• 2009

A new approach for the determination of acrylamide (AM) in foods by solid phase microextraction-gas chromatography (SPME-GC) was established. AM was bromized and transformed to 2-bromoacrylamide (2-BAM). 2-BAM was then extracted by a commercial SPME fiber, $75-{\mu}m$ Car/PDMS fiber, for GC detection. The influence of extraction and desorption parameters such as extraction temperature and time, stirring rate, desorption temperature, and time were studied and optimized. The mass concentration was proportional to the peak area of 2-BPA from 1.0 to 8,000 ${\mu}g/L$. The detection limit of the SPME-GC for 2-BAM was found to be 0.1 ${\mu}g/L$, and the recoveries and relative standard deviations for different food samples were 74.5 to 102.0%, and 4.2 to 9.1%, respectively. The presented method was applied to the determination of AM in fried foods.

• Journal of Electrochemical Science and Technology
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• 제9권2호
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• pp.109-117
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• 2018

A novel carbon paste electrode modified with $Cu-TiO_2$ nanocomposite, 2-(ferrocenylethynyl)fluoren-9-one (2FF) and ionic liquid (IL) (2FF/$Cu-TiO_2$/IL/CPE) was fabricated and employed to study the electrocatalytic oxidation of droxidopa, using cyclic voltammetry (CV), chronoamperometry (CHA) and differential pulse voltammetry (DPV) as diagnostic techniques. It has been found that the oxidation of droxidopa at the surface of modified electrode occurs at a potential of about 295 mV less positive than that of an unmodified CPE. DPV exhibits a linear dynamic range from $5.0{\times}10^{-8}$ to $4.0{\times}10^{-4}M$ and a detection limit of 30.0 nM for droxidopa. Finally this modified electrode was used for simultaneous determination of droxidopa and tryptophan. Also the 2FF/$Cu-TiO_2$/IL/CPE shows excellent ability to determination of droxidopa and tryptophan in real samples.

• 동의생리병리학회지
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• 제17권2호
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• pp.443-446
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• 2003

To investigate the protective effect of Bulbus Allii Macrostemi (BAM) on the damage by pulmonary vascular endothelial cells by xanthine oxidase (XO)/hypoxanthine (HX)-induced oxygen free radical, Neutral Red (NR) and protein kinase c (PKC) activity assay were used. The results were obtained as follows ; The viability of vascular endothelial cells treated with XO/HX was decreased. And activation of PKC represented a maximal increase in group treated with XO/HX for 15 mins in vasvular pulmonary endothelial cells. But pretreated groups with BAM extracts were not inhibited the increase of PKC activation by XO/HX in a dose-dependent fashion. These results show that XO/HX elicits toxic effects in cultured pulmonary vascular endothelial cells, and suggest that BAM extract is very effective in the prevention of XO/HX-induced PKC activation.

• 대한바이러스학회지
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• 제29권2호
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• pp.99-105
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• 1999

BamHI restriction pattern and genomic library of Herpes simplex virus type 2 (HSV-2) strain G were constructed, and locations of the glycoproteins gB and gD, and tk genes on the fragments were detected by Southern blot analysis. HSV-2 genomic DNAs were cleaved into twenty-seven fragments by BamHI enzyme in the range of 0.72 to 15.08 (total 150.44 kb), which were cloned into the BamHI site of pBluescript SK(+) to construct genome library of the HSV-2. The library was named by the order of the fragment size from smallest one to largest one. HSV-2 glycoprotein gD gene was located in pHLA2-21 and pHLA2-22 recombinant plasmids, gB gene in pHLA2-24 plasmid, and tk gene in pHLA2-11 clone by Southern blot analysis.

• 한국전자거래학회지
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• 제12권2호
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• pp.219-231
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• 2007

본 논문에서는 복합 이벤트 처리를 적용하여 비즈니스 활동 분석(BAM)을 위한 이벤트 분석 방안을 제안한다. 이는 프로세스 관리자가 프로세스가 종료되기 이전에 발생 가능한 위험을 감지하고 모니터링하기 위하여 실시간에 진행되는 이벤트에 대하여 조기 경보를 제공하기 위하여 개발되었다. 본 연구에서는 의미 있는 위험을 가지는 이벤트를 추출하는, 프로세스 기반의 이벤트 모니터링 과정을 제시하였다. 복합 이벤트 패턴은 과거 누적된 이벤트 로그를 바탕으로 정의되며, 이벤트의 위험도는 그 패턴들에 기반하여 평가된다. 제안된 방법론은 홈쇼핑업체의 서비스 프로세스의 예를 이용하여 설명한다.

• Advances in environmental research
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• 제1권1호
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• pp.69-82
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• 2012

Based on the measurement data of the particulate matter with an aerodynamic diameter of less than or equal to a nominal 10 ${\mu}m$ (PM10) by the ${\beta}$-ray absorption method (BAM) equipped with an inlet heater and the gravimetric method (GMM) at two coastal sites in Korea, the optimal inlet heater temperature was estimated. By using a gas/particle equilibrium model, Simulating Composition of Atmospheric Particles at Equilibrium 2 (SCAPE2), water content in aerosols was estimated with varying temperature to find the optimal temperature increase to make the PM10 concentration by BAM comparable to that by GMM. It was estimated that the heated air temperature inside the BAM should be increased up to $35{\sim}45^{\circ}C$ at both sites. At this temperature range, evaporation of volatile aerosol components was minor. Similar ($30{\sim}50^{\circ}C$) temperature range was also obtained from the calculation based on the absolute humidity which changed with ambient absolute humidity and chemical composition of hygroscopic species.

• 미생물학회지
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• 제21권4호
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• pp.229-237
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• 1983

Aspergillus nidulans의 tRNA 유전자의 구성과 발현기착을 연구하기 위하여 우선 Aspergillus의 총 tRNA 유전자를 cloning 하였다. Aspergillus의 핵 DNA롱 포자로 부터 분리해 내고 본질 형성에서 분리한 BamHI과 T4 DNA ligase를 사용하여 pBR322플라스미드에 재조합시켜서 cloning하였다. 15벤의 transformation을 하여 30,000개 의 transformants 얻었고, 이 중 Aspergillus DNA를 가지고 있는 colony는 5,300켜개였다. In vivo에 서 S2p로 표지 된 total tRNA를 probe로 하여 colony hybridization 실험 결과, 105개의 total tRNA유전자 clone을 얻었다. 위의 결과와 cohybridization 실험 결과를 분석해 보면, Asprgillus의 tRNA 유전자는 yeast의 그것보다는 좀 더 밀집되어 존재한다고 생각된다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2003년도 춘계학술대회 논문집 센서 박막재료 반도체 세라믹
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• pp.103-106
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• 2003

It is well known that the state of existence of molecules on the surface of water changes during compression of the molecules. Electric methods, such as measurement of the surface potential or displacement current are also useful for investigating dynamic changes of molecular state on the water surface during compression. In this paper, We studied on the Bio thin film by Langmuir-Biodgett(LB) method. The Experiment method used displacement current, $\pi-A$ isotherm and BAM (Brewster Angle Microscopy). using the BAM, we can to the molecular orientation of monolayer on the water surface and directly see the morphology of the films on water subphase as well as that of the films.