A microorganism capable of producing high level of poly-3-hydoxybutyrate (PHB) from xylose was isolated from soil. The isolated strain J-65 was identified as Bacillus megaterium based on the morphological, biochemical and molecular biological characteristics. The optimum temperature and pH for the growth of B. megaterium J-65 were $37^{\circ}C$ and 8.0, respectively. The optimum medium composition for the cell growth was 2% xylose, 0.25% $(NH_4)_2SO_4$, 0.3% $Na_2HPO_4{\cdot}12H_2O$, and 0.1% $KH_2PO_4$. The optimum condition for PHB accumulation was same to the optimum condition for cell growth. Copolymer of ${\beta}$-hydroxybutyric and ${\beta}$-hydroxyvaleric acid was produced when propionic acid was added to shake flasks containing 20 g/l of xylose. Fermenter culture was carried out to produce the high concentration of PHB. In batch culture, cell mass was 9.82 g/l and PHB content was 35% of dry cell weight. PHB produced by B. megaterium J-65 was identified as homopolymer of 3-hydoxybutyric acid by GC and NMR.
Purpose: Proteins are major components of the body and essential nutrients for proper growth and development. However, studies on protein intake in children and adolescents are insufficient. A few previous studies have reported the relationship with growth indicators, but results vary depending on the source of protein. Therefore, the current study investigates the relationship between protein intake and overweight and obesity among children and adolescents in Korea. Methods: Based on the 2014-2019 Korea National Health and Nutrition Examination Survey, 5,567 children and adolescents aged 6-18 years, who participated in a 24-hour dietary recall with information on height and weight, were included in this study. Protein intake was estimated as percentage of total energy (% of energy) and was classified into animal and plant protein according to the food source. Overweight and obesity were defined using the 2017 pediatric and adolescent growth chart. Results: Total protein intake of the subjects was estimated as 14.5% of total energy (animal protein 8.3% and plant protein 6.3%). The group with the highest total protein intake had a higher odds ratio (OR) of overweight/obesity than those with the least protein intake (OR, 1.36, 95% confidence interval (CI), 1.10-1.67, p for trend = 0.003). When classified by food source, the group with the highest animal protein intake had a significantly higher OR of overweight/obesity than subjects with the lowest intake (OR, 1.30, 95% CI, 1.05-1.61, p for trend = 0.016). However, plant protein was not significantly associated with overweight/obesity. Conclusions: These findings suggest that a high intake of animal protein in children and adolescents increases the risk of being overweight and obese. In order to develop normal growth and prevent obesity in the future, it is necessary to determine an appropriate protein intake level through nutrition education programs and prospective studies on balanced protein intake.
Asia-Pacific Journal of Business Venturing and Entrepreneurship
/
v.7
no.4
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pp.113-122
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2012
A success of opening a business should be supported with complete preparations and implementation. While there have been various educational programs for opening a business to support its success, there have not been a lot of studies on the relationships between educational programs and operational performance in small businesses, due to the difficulties of empirical survey. In this point, this study attempts to understand the relationships between preparation of opening a business and operational performance, while dividing samples into two groups (1. a group completing educational programs for opening a business, 2. a group non-completing). For the perspectives of the preparation of opening a business, various success factors have been suggested through various studies, but the perspectives and approaches of balanced business operation have been required. Therefore, using BSC, it developed a framework with the four perspectives of preparation for opening a business, and empirically reviewed the relationships between the four perspectives and operational performances in small businesses. It conducted a survey on the total 252 samples, whose business periods are less than 5 years. As the results, the preparations of financial and customer/marketing perspectives have significant relationships between operational performances in total samples, preparations of financial, customer/marketing, and growth-strategies perspectives in the educational program completing group and only preparation of financial perspectives in the non-completing group have significant relationship with operational performances. The differences between the two groups may imply that educational programs for opening a business may be helpful for preparation of opening a business and have some possibility of direct and indirect effects for the success of opening a business. Also, the developed BSC based-preparation framework for opening a business is meaningful for the view of balanced business operations, and can contribute to develop the educational programs for opening a business.
This study was conducted to investigate the effects of concentrated pig slurry and byproduct liquid fertilizer on the growth and yield of chinese cabbage. The experiment was conducted in a rain-shelter house which was installed in the agriculture farm. Plants were fertilized with concentrated slurry (CS), byproduct fertilizer (BF), mixture of concentrated slurry and byproduct liquid fertilizer (CS+BF), combined organic and chemical fertilizer (CS+BF+BF) and chemical fertilizer (CF) as control. 1. The pH level of byproduct liquid was decreased from the 3rd to the 7th day and increased 9 day to 14th day, but pH of concentrated slurry (CS) was not greatly varied. EC of concentrated slurry (CS) and byproduct liquid was increased gradually during the fermentation. 2. The concentrated slurry (CS) was low in phosphorus, calcium, magnesium, rich in potassium and unbalanced as a low nitrogen and high potassium. But byproduct liquid fertilizer was balanced in nitrogen and potassium ratio. 3. The leaf number, head height, head width of chinese cabbage in treatment with organic and chemical fertilizer (CS+BF+N) showed significant difference compared with control. The plant and head weight of chinese cabbage in treatment of concentrated slurry was severely decreased, but that in treatment organic and chemical fertilizer (CS+BF+N) were increased 8, 10% compared with control chemical fertilizer (CF), respectively. 4. The content of $K_2O$ in plant tissue and in soil was increased after using concentrated slurry. On the other hand, mineral content of except $K_2O$ did not differ significantly between any of the treatments. In conclusion, organic and chemical fertilizer (CS+BF+N) could improve growth and head weight of chinese cabbage.
This article aims to review the recent, especially focusing on the year of 2016, situations and prospects of the Vietnamese politics, economy, and international relations. Politically, Vietnam completed the election of members for the National Assembly and organized new leadership at the 12th National Congress of the Vietnamese Communist Party in 2016. One characteristic of the new leadership is that the politicians, especially the members of politburo, from the North continue to occupy the position of majority. The other one is that the new leadership promised to carry out the restructuring of economy toward industries producing higher value-added commodities even though Vietnam industries admittedly need to accelerate present industrialization and modernization as a developing country under the seemingly contradictory slogan of "the development of market economy for socialism." The declared goals of the new leadership in Vietnam are inevitable in a sense since the development of Vietnamese economy has been heavily dependent upon Foreign Direct Investment(FDI) taking advantage of Vietnamese cheap labor and simultaneously it is evident that its future is unsecure if it maintains status quo. In fact, the Vietnamese economy has impressively showed high growth rate by the help of foreign capitals since 1990s despite the repetitive recessions of global economy but its growth is not likely to be sustainable anymore if it will not reduce foreign dependency and social economic inequality in a long term. In a short run, global economic recession, the financial and monetary policies of global powers, and recent protectionism and uncertainty of trade agreements will be three crucial variables to affect Vietnamese economy. In terms of international relations, Vietnam is continuously expected to practise the policy of checks and balances among the powerful countries. Vietnam has seriously disputed with China on islands sovereignty in the South China Sea and attempted to maintain close relationship with other powerful countries including especially America. However, mainly due to the new protectionism by the regime of American president Donald Trump, the Vietnamese government also need to keep close relationship with China increasingly for both economic and diplomatic security. Under the circumstances, Vietnam is expected to maintain more practical and balanced international relations.
Logistics needs in Asia are surprisingly increasing due to rapid growth of China in Northeast Asia We are endeavoring to make an economic block in opposition to NAFTA and EU by economic revival, political stability, and easing of exclusive parts between countries as increasing of traffic infra such as asian highway, transcontinental railway in the region of Northeast Asia and as considering diversification of transportation mode. Now, Busan is not a final terminal of this business, and it is not a concreted level in considering an undersea tunnel between Korea and Japan as effort to extend to Japan. Therefore, this study performed an effect analysis which be affected to Northeast Asia and Korea in future by analysis and comparison of a cost and time required of marine transportation and railway before and after construction through course analysis and summary of discussed content until now for undersea tunnel between Korea and Japan. This result expects as a accelerative play for study cf undersea tunnel between Korea and Japan in future. In this result, we estimate that it is an effective things in a cost and time required if undersea tunnel between Korea and Japan is constructed, and we simply searched for theirs effects in the aspects of Northeast Asia, national balanced development, regional economic in Busan, and marine logistics.
The purpose of this research is to introduce the fundamental data to develop a program to educate parents properly. To do so, the research investigates the relationship between the father's personal variables and the child's creative personality and emotional intelligence, and searches for what variable shave a significant influence on the balanced growth of a child among a father's personal variables. The participants of study were 200 children of 3~5 years old enrolled in kindergarten or child care centers located in mostly DaeGu city and GyeongBuk provinces. The research applied two tools by measuring the child's creative personality, and observing test paper for a child's emotional intelligence. The data was analyzed by the ANOVA test, Scheff$\acute{e}$ s post-hoc test, Pearson's product moment correlation test and multiple regression analysis. In subordinate variables of a child's creative personality, significance difference was discernable partly with the father's salary and education levels. However, there was no significance difference with the father's age. Especially, the positive influence of the father's education level is apparent on the entire child's creative personality variables, uniqueness and imaginative amusement. Influence Emotional self-awareness shows on entire creative personality variables, uniqueness, diverse interest and imaginative amusement. Emotional awareness in others influences on diverse interest, imaginative amusement. Others emotional control influences positively on entire creative personality. The results of this study suggest that the child's emotional intelligence are significant method to improve the child's creative personality in the education field.
Raj, St.;Fandrejewski, H.;Weremko, D.;Skiba, G.;Buraczewska, L.;Zebrowska, T.;Han, In K.
Asian-Australasian Journal of Animal Sciences
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v.13
no.6
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pp.817-823
/
2000
Four groups of six growing gilts each were fed ad libitum diets composed of cereals and soyabean (SBM) or rapeseed (RSM) meal and containing two levels of crude protein: high - 18.0% (RSM-H) and 16.9% (SBM-H) or low - 15.6% (RSM-L) and 15.1% (SBM-L). The diets were balanced by supplementation with crystalline amino acids and contained apparent ileal digestible lysine, methionine, threonine and tryptophan in proportions (1.00:0.32:0.57:0.18) according to CVB (1995). Voluntary feed intake, weight gain and slaughter and chemical body composition of animals were assessed. Protein and energy balances from 25 to 70 kg body weight were calculated by the comparative slaughter method. Protein source had a significant effect on voluntary feed intake; it was 0.12 kg/d lower in pigs fed the SBM than RSM-diets. Pigs fed on the SBM-L diet consumed the least amount of feed (2.17 kg). Daily gain (average, $900{\pm}12.59g$) and feed conversion ratio ($2.54{\pm}0.04kg/kg$) were not statistically affected by source (SMB and RSM) and protein level (high and low). In empty body similar amounts (g/kg) of protein (163 g), water (635 g) and ash (28 g) were found. However, pigs fed the RSM-L diet were fatter than those fed the SBM-L diet (188 vs. 161 g/kg). No statistical differences were observed in daily protein deposition, which on average amounted to $142{\pm}11g$, or carcass characteristics. An improvement of crude protein utilization by 6.3 percentage units was found by decreasing the protein concentration in the diets. Heat production in the body was not significantly affected by the treatments.
KSCE Journal of Civil and Environmental Engineering Research
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v.33
no.6
/
pp.2529-2539
/
2013
The Korean construction industry has led the miraculous economic boost of Korea by providing solid domestic infrastructures such as highway, roads, and airports. It also played a critical role in global construction market and eaned more than 500 billions dollars in terms of their accumulated international orders. However, domestic construction market has significantly decreased in recent years due to the domestic political environments and global economic crisis. Therefore, the importance of international construction market cannot be more emphasized to the Korean construction market in order for the sustainable growth. There has been, however, little research in the area of identifying required competency elements for the Korean construction industry to stay successful in the global market. The main purpose of this study is to identify elements of core competency to increase global competitiveness for Korean construction industry. Core global construction competency elements were derived from the internal and external environmental analyses along with the extensive literature review, expert interviews and a survey. This study utilized the Importance-Performance Analysis (IPA) and a gap analysis in providing insights on the status competitiveness of the Korean construction industry in terms of required global core competency elements. The analysis shows that project management and financial management are the main areas for improvements required to engineering contractors while construction contractors need to take a more balanced approach among technical, project management, and financial management in order to increase their global competencies.
Giang, Hoang Huong;Viet, Tran Quoc;Ogle, Brian;Lindberg, Jan Erik
Asian-Australasian Journal of Animal Sciences
/
v.24
no.5
/
pp.655-661
/
2011
Two experiments were conducted to investigate the effect of dietary supplementation of Bacillus, Saccharomyces and lactic acid bacteria (LAB) on performance and nutrient digestibility in grower and finisher pigs. In Exp. 1, 80 pigs (32 females and 48 males), $28.7{\pm}0.9\;kg$ body weight (BW), were randomly divided into 4 treatment groups balanced for sex and weight (5 pigs per pen, 4 pens per treatment). They were fed one of four diets: a basal grower (20-50 kg BW) and finisher (>50 kg BW) diet without any addition of probiotic or antibiotic (diet C), the basal diet supplemented with Bacillus subtilis H4 (diet B), diet B supplemented with Saccharomyces boulardi Sb (diet BS) and diet BS supplemented with a LAB complex (diet BSL). The LAB complex consisted of Enterococcus faecium 6H2, Lactobacillus acidophilus C3, Pediococcus pentosaceus D7, and Lactobacillus fermentum NC1. In Exp. 2, 16 male pigs, $29.2{\pm}0.8\;kg$ BW, were kept in individual pens and divided into 4 groups (4 pigs in each group). All 4 groups were given exactly the same growing-period diets (diet C, B, BS and BSL) as in Exp 1. The total faeces and urine were collected during 5 days (day 20-24) to determine nitrogen retention and total tract digestibility. In the growing period, average daily feed intake (ADFI), average daily gain (ADG) and feed conversion ratio (FCR) were not affected by diet B and BS (p>0.05), but ADG increased (+5.9%) (p<0.05) and FCR improved (+5.9%) (p<0.05) on diet BSL compared with the control, although ADFI was not different (p>0.05). Digestibility of crude protein and organic matter was higher (p<0.05) in diet BSL and digestibility of crude fibre was higher (p<0.05) in diet BS and BSL than in diet C. Nitrogen retention was not affected by diet (p>0.05). The faecal LAB counts were increased in grower pigs fed diet BSL (p<0.05) and faecal E. coli counts were decreased in pigs fed diets BS and BSL (p<0.05). In the finishing period, no effects of diet were found in ADFI, ADG, FCR, nutrient digestibility, and nitrogen retention (p>0.05). Faecal LAB and E. coli counts in the finisher pigs were not affected by diet (p>0.05). In conclusion, the current study demonstrates that a mixture of bacteria and yeast has the potential to be used as a probiotic dietary supplement in grower pigs.
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