• KSBB Journal
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• v.19 no.4
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• pp.245-249
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• 2004

A combined method of autolysis and enzymatic hydrolysis of baker's yeast was developed for the production of yeast extract, which is widely used as a natural food ingredient. From statistical analysis, NaCl and ethanol addition were found to be significantly effective factors in autolysis of yeast. The optimum dosages of salt and ethanol were 3% and 1%, respectively. Heat treatment and the use of cell lytic enzyme were not significantly effecting on the autolysis. Yeast hydrolysate was prepared by autolysis, followed by enzymatic hydrolysis using proteases, nuclease and deaminase. Additionally, the hydrolysate was processed by downstream process including Maillard reaction and debittering. The total dry matter yield and total nitrogen yield for the process were 76% and 59%, respectively. Compared to a process using brewer's yeast, when baker's yeast was used as a raw material, a higher recovery yield was obtained.

• Honam Mathematical Journal
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• v.45 no.4
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• pp.572-584
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• 2023

In this study, we search for Pell and Pell-Lucas numbers, which are concatenations of two repdigits and find these numbers to be only 12, 29, 70 and 14, 34, 82, respectively. We use Baker's Theory and Baker-Davenport basis reduction method while finding the solutions.

• Honam Mathematical Journal
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• v.42 no.1
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• pp.139-150
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• 2020

Let p be a prime number with p > 3, p ≡ 3 (mod 4) and let n be a positive integer. In this paper, we prove that the Diophantine equation (5pn² - 1)^x + (p(p - 5)n² + 1)^y = (pn)^z has only the positive integer solution (x, y, z) = (1, 1, 2) where pn ≡ ±1 (mod 5). As an another result, we show that the Diophantine equation (35n² - 1)^x + (14n² + 1)^y = (7n)^z has only the positive integer solution (x, y, z) = (1, 1, 2) where n ≡ ±3 (mod 5) or 5 | n. On the proofs, we use the properties of Jacobi symbol and Baker's method.

• KSBB Journal
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• v.15 no.2
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• pp.134-138
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• 2000

Addition of carbon and nitrogen source to an alcohol distillery wastewater was tried to increase the cell concentration of a b baker's yeast cultivated in that wastewater. Carbon was found to be primary limiting nutrient and nitrogen secondary limiting o one. Glucose addition increased the cell concentration 1.3 times higher than no addition, and both glucose and $(NH_4)_2S0_4$ a addition did 5.8 times. A fed-batch cultivation by the automatic addition of glucose and ammonium was executed. Added g glu$\infty$se was automatically controlled to low concentration by a method using DO as control parameter. Ammonium was a automatically added as NH40H used as pH $\infty$ntrol agent after initiating glucose addition. By this simple cultivation method t the cell concentration $\infty$내d be efficiently increased from 2.6g/L to 12.0g/L, and maximum specific growth rate and biomass y yield to glu$\infty$se were $0.18hr^{-1}$ and about 0.54g/g respectively. By increasing cell concentration, COD of the wastewater m media could be additionally reduced by about 22%.

• KSBB Journal
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• v.15 no.2
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• pp.139-144
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• 2000

Automatic addition of glucose and ammonium to an alcohol distille깨 wastewater and the control of them at low $\infty$ncentration were tried to efficiently increase the cell concentration of a baker's yeast c비tivated in that wastewater. Added g glucose was indirectly controlled to less than 116 mg/L by a method which used DO as control parameter. Ammonium was a automatically measured and controlled within the range of 7.0~27.7 mM by a homemade on-line system which adopted FIA a as measurement method. Maximum specific growth rate and biomass yield to glucose were $0.21 hr^{-1}$ and about 0.78 g/g, w which were significantly increased values in contrast to those of an experiment without ammonium control. Biomass yield to a ammonium was 11.3 gIg. Cell cone엉ntration could be increased from 2.6 g/L to 18.5 g/L by the add ion of glucose and a ammonium.

• KSBB Journal
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• v.18 no.3
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• pp.197-201
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• 2003

Automatic addition of glucose, ammonium and phosphate to alcohol distillery wastewater and their control at low concentrations have been carried increase the cell concentration of a baker's yeast cultivated in the wastewater. Glucose was automatically added using dissolved oxygen as the control parameter, and maintained below 300 mg/L. Ammonium was automatically added by a pH-stat method and maintained in the low range of 12.6~17.4 mM. An automated FIA system, which used an ascorbic acid-based method was developed for the automatic analysis nad addition of phosphate. With this system, the phosphate concentration was succesfully analysed and controlled afrer 19.4 hr in the range 23.3~43.4 mg/L. The cell concentration was increased by 33.0-fold by the addition of these three nutrients. The overall specific growth rate of the yeast was 0.19 $hr^{-1}$.

• KSBB Journal
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• v.26 no.1
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• pp.87-91
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• 2011

Automatic counting of yeast cells in baker's yeast culture was tried using a conventional light microscope equipped with a pc camera. Relatively good binary image was obtained by using white LED as microscope light source, but uneven brightness distribution in original image hindered counting accuracy. A block binarization method using local thresholds proportional to local brightnesses was used to get improved binary images. The brightnesses of the blocks were expressed as the value component in HSV color model. Good quality binary images were obtained by binarization on $8{\times}6$ blocks of original images and connected-component labelling of the binarized images produced reliable counting results in the concentration range $1.4{\times}10^5/mL{\sim}1.4{\times}10^7\;cells/mL$.

• Earthquakes and Structures
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• v.7 no.2
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• pp.141-157
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• 2014

The Conditional Mean Spectrum represents a powerful link between the seismic hazard information and the selection of strong ground motion records at a particular site. The scope of the paper is to apply for the city of Bucharest for the first time the method to obtain the Conditional Mean Spectrum (CMS) presented by Baker (2011) and to select, on the basis of the CMS, a suite of strong ground motions for performing elastic and inelastic dynamic analyses of buildings and structures with fundamental periods of vibration in the vicinity of 1.0 s. The major seismic hazard for Bucharest and for most of Southern and Eastern Romania is dominated by the Vrancea subcrustal seismic source. The ground motion prediction equation developed for subduction-type earthquakes and soil conditions by Youngs et al. (1997) is used for the computation of the Uniform Hazard Spectrum (UHS) and the CMS. The disaggregation of seismic hazard is then performed in order to determine the mean causal values of magnitude and source-to-site distance for a particular spectral ordinate (for a spectral period T = 1.0 s in this study). The spectral period of 1.0 s is considered to be representative for the new stock of residential and office reinforced concrete (RC) buildings in Bucharest. The differences between the Uniform Hazard Spectrum (UHS) and the Conditional Mean Spectrum (CMS) are discussed taking into account the scarcity of ground motions recorded in the region of Bucharest and the frequency content characteristics of the recorded data. Moreover, a record selection based on the criteria proposed by Baker and Cornell (2006) and Baker (2011) is performed using a dataset consisting of strong ground motions recorded during seven Vrancea seismic events.

• Transactions on Control, Automation and Systems Engineering
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• v.4 no.4
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• pp.277-282
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• 2002

This paper proposes a nonlinear adaptive controller based on back-stepping method for tracking reference substrate concentration by manipulating dilution rate in a continuous baker's yeast cultivating process in stirred tank bioreactor. Control law is obtained from Lyapunov control function to ensure asymptotical stability of the system. The Haldane model for the specific growth rate depending on only substrate concentration is used in this paper. Due to the uncertainty of specific growth rate, it has been modified as a function including the unknown parameter with known bounded values. The substrate concentration in the bioreactor and feed line are measured. The deviation from the reference is observed when the external disturbance such as the change of the feed is introduced to the system. The effectiveness of the proposed controller is shown through simulation results in continuous system.

• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.74.3-74
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• 2001

This paper proposes a nonlinear adaptive controller based on back-stepping method for tracking reference substrate concentration by manipulating dilution rate in a continuous baker´s yeast cultivating process in stirred tank bioreactor. Control law is obtained from Lyapunov control function to ensure asymptotical stability of the system. The Haldane model for the specific growth rate depending on only substrate concentration is used in this paper. Due to the uncertainty of specific growth rate, it has been modified as a function including the unknown parameter with known bounded values. The substrate concentration in the bioreactor and feed line are measured. The deviation from the reference is observed when the external disturbance such as the change of the feed is introduced to the system ...