• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.434-442
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• 2008

We observed that an inclusion body (IB) of recombinant ${\beta}$-galactosidase that was produced by the araBAD promoter system in Escherichia coli (E. coil) showed enzyme activity. In order to improve its activity, the lowering of the transcription rate of the ${\beta}$-galactosidase structural gene was attempted through competition between an inducer (L-arabinose) and an inducer analog (D-fucose). In the deep-well microtiter plate culture and lab-scale fermentor culture, it was demonstrated that the addition of D-fucose caused an improvement in specific ${\beta}$-galactosidase production, although ${\beta}$-galactosidase was produced as an IB. In particular, the addition of D-fucose after induction led to an increase in the specific activity of ${\beta}$-galactosidase IB. Finally, we confirmed that the addition of D-fucose after induction caused changes in the structure of ${\beta}$-galactosidase IB, with higher enzyme activity. Based on these results, we expect that an improved enzyme IB will be used as a biocatalyst of the enzyme bioprocess, because an enzyme IB can be purified easily and has physical durability.

• Journal of Navigation and Port Research
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• v.29 no.7
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• pp.641-646
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• 2005

In cargo-working, it unavoidably happens that the quay crane slip along the rail and the container move from side to side. Especially, they involve a lot of risk in bad weather. The rail clamp is a mooring device to prevent that the quay crane slips along the rail due to bad weather or the wind blast while the quay crane do the cargo-working And it will play a greater role in port container terminal integration and automation To design the wedge type rail clamp, it is very important to determine the wedge angle. In this study, we expect that the design wind speed of the quay crane will change over 16m/s. Assuming that the design wind speed is 40m/s, we determined the proper wedge angle of the wedge type rail clamp for the 50ton class quay crane.

• Journal of the Korea Institute of Building Construction
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• v.5 no.1 s.15
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• pp.63-68
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• 2005

Generally, aggregate may limit the workability, strength and durability of concrete, and good concrete cannot be made with aggregate of bad property including low strength, bad shape and grading. But recently, it has been insufficient in quantity to collect good natural aggregate because of exhaustion of aggregate resources. In case of Korea, the using ratio of crushed stone occupies about 97 percent of total coarse aggregate, and ratio of crushed sand occupies about 18.3 percent of total fine aggregate. This is an experimental study to compare and analyze the properties of crushed sand for concrete in capital region and concrete according to the replacement ratio of crushed sand to improve quality and mix design of concrete using crushed sand. According to test results, it was found that nearly all the properties of crushed sand satisfied with the value recommended by KS. And it is recommended that FM of crushed sand should be lowered by improvement of manufacture system or grading adjustment should be used because FM of crushed sand was a bit higher.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.24 no.1
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• pp.61-69
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• 2012

Recently, tower type apartment has been a main stream at the apartment market, in Korea. Tower type apartment has features that is easy to maintain a proper distance between apartment buildings, and to keep a prospect comparing to flat type apartment However, there is usually a inner curved area at tower type apartment, and it is able to make an unhealthy environment because of shaded area through a day and a bad air flow. In this study, the duration of sunshine and the exterior air flow environments are examined. As results, the duration of sunshine at the inner curved area of tower type apartment was extremely short, and the exterior air flow environment was very bad for the low air velocity of about 0.08m/s. Installing pilotis in the 1st floor which faces to the inner curved area makes the good improvement of exterior air flow environment.

• Journal of International Academy of Physical Therapy Research
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• v.1 no.1
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• pp.26-31
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• 2010

The purpose of this study was to investigate perceived health status, activities of daily living and depression of the elderly in nursing facilities and to identify correlations among them. The collected data is to improve healthy life for the aged people in communities. This study was performed by using of questionnaire which was consisted of perceived health status, activity of daily living(ADL) and depression. The survey was conducted by 180 aged people at nursing homes. The results of perceived health status show that 64.9% of elderly feel very bad or bad, 61.6% of elderly have a degree of independent level of activity of daily living(ADL) and 48.6% of elderly have a degree of depression. There were statically revealed meaningful correlation between ability of activity of daily living(ADL) and perceived health status, ability of activity of daily living(ADL) and depression. This study about connection among perceived health status, activity of daily living(ADL) and depression is necessary for number of the affiliation function of elderly at nursing homes and development of intervention programs concerned about depression are necessary.

• The Journal of Fisheries Business Administration
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• v.31 no.1
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• pp.1-12
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• 2000

The experience of many countries strongly suggests that bad governments and institutions have been a serious, if not the most serious, obstacle to economic growth and industry-structural adjustments. All public sectors pursue a mix of both predatory and productive activities-bad governments emphasizing the former, while good governments finding a way of promoting the later. In fishery public policy studies, much confusion exists about the roles of policy illustration and prescription. In general fishery public sectors involve collective actions by numerous individuals under conditions of uncertainty, complexity, bounded rationality, and imperfect information structure. All collective fisheries action organizations consist of a center(e.g., government), which leads fishery group actions, and peripheral participants(e.g., fishermen), which are controlled by the government. A paradigm is developed that gives both theoretical and empirical meaning to the constitutional determination of fisheries political preference function or fishery public sector governance structures. Three relevant spaces are specified: policy instrument, results, and constitutional. The collective-choice rules of the constitutional space structure the tradeoff between public and special fishery interest groups. Fishery public sectors seeking sustainable reductions in wasteful rent-seeking fishing activities should select constitutional principles and institutional structures that tend to promote resource sustainability. In particular, the effects of internal and external events on fisheries may result in a greater or lesser concentration of interest group power. Thus, the structure of the fishereis political power must be assessed in any prescriptive evaluation of alternative fishery governance weights.

• Journal of the Korean Society of Safety
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• v.30 no.4
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• pp.106-112
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• 2015

For the construction safety manager, it is expected that job stress is significantly high due to lack of expertise about new technology, new construction methods, and enlargement of scale as well as due to work intensity and unstable work hours. It is probable that such an increase in job stress not only has bad effects on individual, but also the bad effects spread to construction industry at large, resulting in rise in accident rate, increase in direct and indirect loss cost, and loss of reputation. In this context, methods for controlling construction safety manager's job stress are needed at the levels of both government and company. Therefore, this study investigated and analyzed the effects of construction safety manager's job stress on job satisfaction and job commitment, and proposed methods for relieving job stress. In addition, this study was suggested policies for higher job satisfaction and job commitment at the levels of the government and companies.

• Journal of the Korean Society of Costume
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• v.30
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• pp.139-152
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• 1996

THe purpose of this study is to clarify Kitsch in the modern society and to analyze the modern fahion design adapting Kitsch's materiality. Thus this study explains the concepts and background of kitsch and examines its aes-thetic value in consideration of the relation be-tween Kitsch and plastic art. Through a theoretical examination of histori-cal documents the meaning of the Kitsch is explored from various angles. The result of this study were as follows: Kitsch began in the period of the 19th cen-tury when was society began to form and so-ciety grew to be rich. This is to say Kitsch is the result of intrusion of capitalist modernity in the domain of the aesthetic modernity The concept meant ethical something bad counterfeit and bad taste. The materialities of Kitsch are over-decor-ation unfitness the use of imitation malfunc-tion used fashion design. 1. Over-decoration is due to the material abundnce through various accessories many colors etc. 2. Unfitness is obtained by the error of ar-rangement which means the wrong cohesion of form size of material. 3. The use of imitation makes use of cheap materals such as vinyl imitation leather imi-tation fur etc. 4. Malfunction comes from losing the orig-inal function by the addition of un-neccessary functions.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.6
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• pp.86-95
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• 2003

The object of this paper is to correct check the tree situation and quantity around highway. Also, those data utilize in order to establish plan about how to the long and short term landscape construction and maintain program. The result of this study can be summarized as follows; 1. Tree decrease rates for 8 branch offices were Jongbu(5.62%), Gangwon(4.32%), Chungcheong (3.35%), Honam(5.62%), Gyeongbuk(3.06%), Gyeongnam(5.60%), Seorak training center(0.31%), Headquarter(1.54%). Also decrease causes were traffic accidents(1.8%), air po11ution(4.7%), humid damage(0.9%), insect and disease(1.2%), wind and rainfall(3.4%), dry damage(3.5%), cold damage (1.0%), fire(3.1%), damage of the man and anima1(4.1%), remove bad tree(13.1%), bad rooting(9.5%) and etc.(53.7%). 2. Improve methods of tree death problems were regulation management(ferti1ize, irrigation and pesticide work), improvement of draining system, Pull out the weeds, Plant native plants, utilize organic matter fertilize and plant environment trees.

• Language and Information
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• v.19 no.2
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• pp.127-153
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• 2015

Negative expressions have their semantic function of classical negation as a pure reverser of truth-values. They also have various kin and foes of their pragmatic functions such as association of bad feelings (Russell 1948), emphasis/attenuation by negative polarity items, sarcasm, and metalinguistic negation (MN). This paper explores how MN and descriptive negation (DN) differ and whether the difference creates pragmatic ambiguity (Horn 1987) or reflects merely contextual variations of one logical negation (Carston 1996). To test the debate, this paper treats certain degree modifiers licensed exclusively by MN as in Mia-ka POTHONG/Yekan yeppu-n key an-i-a [external neg] (vs. modifier NPIs like cenhye 'at all', licensed only by DN) and contrasts them with bad utterances of the MN modifiers in [short form neg] sentences (not for MN) such as Mia-ka POTHONG an yeppu-e. The ERP results of the well-formed vs. ill-formed conditions evoked the N400 at Cz in written stimuli and the N400 near the center on both hemispheres in spoken stimuli. The results suggest that the anomalies are meaning-related and tend to support the pragmatic ambiguity.