• Journal of Microbiology and Biotechnology
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• v.1 no.3
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• pp.212-219
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• 1991

Four species of yeast, Saccharomyces cerevisiae, Candida utilis, Saccharomycopsis flbuligera, and Schwanniomyces castellii were evaluated for their ability to bioconvert potato processing waste water into microbial protein and the resulting single-cell proteins were evaluated as protein sources for rainbow trout, using in vitro analyses. The studies indicated that Schwanniomyces castellii, which utilizes starch dircetly and converts it into cell mass efficiently, was suitable for the bioconversion. In the single-stage continuous bioconversion, the yield S. castellii cell mass, which contained approximately 37% protein, was 77%, at dilution rate 0.25 $h^{-1}$. Reduction of total carbohydrate was 81%. During batch fermentations, cell mass yield was about 72% and total carbohydrate reduction was 81%. Among the yeasts tested, S. castellii possessed the most fragile cell wall and had a favorable amino acid profile for salmonid fish; protein score of 86% (Met). In an in vitro pepsin digestibility test 80% digestibility (23~38% above control) was observed when cells were pre-heated in a steam bath for 30 min. Results presented should be regarded as being preliminary in nature because they were derived from single experiments.

• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1714-1727
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• 2015

This work describes a new strategy for optimal design of Multiplex-PCR primer sequences. The process is based on the Particle Swarm Optimization-Simplex algorithm (Mult-PSOS). Diverging from previous solutions centered on heuristic tools, the Mult-PSOS is selfconfigured because it does not require the definition of the algorithm's initial search parameters. The successful performance of this method was validated in vitro using Multiplex-PCR assays. For this validation, seven gene sequences of the most prevalent bacteria implicated in urinary tract infections were taken as DNA targets. The in vitro tests confirmed the good performance of the Mult-PSOS, with respect to infectious disease diagnosis, in the rapid and efficient selection of the optimal oligonucleotide sequences for Multiplex-PCRs. The predicted sequences allowed the adequate amplification of all amplicons in a single step (with the correct amount of DNA template and primers), reducing significantly the need for trial and error experiments. In addition, owing to its independence from the initial selection of the heuristic constants, the Mult-PSOS can be employed by non-expert users in computational techniques or in primer design problems.

• Korean Journal of Veterinary Research
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• v.24 no.1
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• pp.50-57
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• 1984

Tuberculin(HCSM) 반응유우 60두에 대하여 가열살균처리한 우결핵균에서 얻은 PPD-BS tuberculin을 이용한 추벽피내재검사법과 석탄산살균처리한 우결핵균에서 얻은 SFA tuberculin을 이용한 경측피내재검사법간의 민감성과 특이성을 비교하였다. 또한 SFA tuberculin과 현행 조형 PPD(PPD-A) tuberculin을 이용한 비교검사법의 가치를 판독기준에 따라 분석하였다. 병소우군에서 PPD-BS와 SFA tuberculin간에 민감성은 차이가 없었으나 무병소우와 감염우동거군에서 SFA tuberculin은 PPD-BS tuberculin에 비하여 비특이반응이 현저히 낮았다(P<0.01). SFA와 PPD-A를 이용한 비교검사법은 판독기준에 따라 가양성반응과 가음성반응에 크게 영향을 주었으며, 피내반응차이 4mm를 판독기준으로 할 때 병소우와 무병소우를 감별할 수 없었다. 이 연구에서 SFA tuberculin은 PPD-BS에 비하여 병소우에서 민감성간에는 차이가 없었으나 무병소우에서 특이성이 현저히 높았다는 점으로 보아 앞으로 HCSM tuberculin 반응우에 대한 재검사는 현행 PPD에서 SFA tuberculin으로 대체함으로써 비특이반응우를 더욱 감소시킬 수 있다는 것을 의미한다.

• Journal of Life Science
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• v.23 no.9
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• pp.1133-1139
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• 2013

During the collection of boar semen, bacterial contamination usually occurs. The contamination has deleterious effects both on semen quality and on sow fertility. The majority of contaminants are gram-negative bacteria, especially Serratia marcescens. In this study, we developed a PCR assay for the identification of S. marcescens targeting the luxS gene (GenBank no. EF164926). S. marcescens yielded a specific 306 bp PCR product. However, no amplification was observed in the other strains tested. The detection limit of PCR was $50pg/{\mu}l$ of template DNA of S. marcescens. The antimicrobial susceptibility patterns of S. marcescens isolated from boar semen were tested using the disk diffusion method. Gentamicin, ceftiofur, florfenicol, and neomycin showed high sensitivity in this test. The minimum inhibitory concentration (MIC) was also determined by the broth microdilution method. The $MIC_{90}$ values of ceftiofur, enrofloxacin, gentamicin, and neomycin were 8, 8, 8, and $16{\mu}g/ml$, respectively. These results indicate that PCR amplification of the luxS gene is a reliable and effective method for the identification of S. marcescens and that ceftiofur, enrofloxacin, gentamicin, and neomycin are effective semen extenders for controlling S. marcescens.

• Korean Journal of Veterinary Research
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• v.38 no.2
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• pp.345-352
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• 1998

The diagnosis of brucellosis is currently based on serological and microbiological tests. However, the microbiological isolation and identification have several disadvantages such as time-consuming and laborious, and the serological methods have been reported to cross-react with antigens other than those from Brucella spp. To develop a sensitive and rapid diagnostic method for detection of Brucella species, the genus-specific primers were designed and synthesized from the sequence of gene encoding a 31kDa cell surface protein(BCSP) and a 36kDa outer membrane protein(OMPB) of B abortus. The amplified 711bp and 982bp DNA fragments were only visible in each species of Brucella by PCR method using the BCSP and OMPB primers, respectively. However, PCR product was not obtained with DNA from other Gram-negative bacteria. As little as 1pg of the B abortus genomic DNA could be detected by this PCR method. Using the PCR technique, semen samples from 185 bulls of Brucella-seronegative herds in Cheju island were examined for comparison of this PCR method with conventional methods in 1995. The semen samples from 5 bulls were positive by culture method and PCR, and one was positive and 5 were suspect by semen plasma agglutination test. However, the semen samples obtained from 177 bulls were negative by semen plasma agglutination, culture and PCR methods in 1996. The results of comparison tests suggested that PCR was a better test than agglutination test against semen of bulls. This study indicated that the PCR technique was a valuable for the diagnosis of bovine brucellosis, particulary in bull semens.

• The Journal of the Korean dental association
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• v.19 no.10 s.149
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• pp.835-837
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• 1981

• Proceedings of the Microbiological Society of Korea Conference
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• 2003.05a
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• pp.73-76
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• 2003

• The Journal of the Korean Society for Microbiology
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• v.1 no.1
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• pp.28.2-29
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• 1958

• Proceedings of the Microbiological Society of Korea Conference
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• 2007.05a
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• pp.71-73
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• 2007

• Proceedings of the Microbiological Society of Korea Conference
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• 2007.05a
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• pp.22-23
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• 2007