• 미생물학회지
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• 제37권3호
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• pp.209-213
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• 2001

Bacillus spp. 이용하여 B3공법을 사용하는 하수 종말 처리장의 포기조에서 Bacillus sup. 변화를 FSIH방법과 포자염색 방법으로 관찰하였다. DAPI로 측정한 세균수는 $3.2{\times}10^8 ~ 4.4{\times}10^8 $ cells/ml 범위로 큰 변화가 없었으나, Bacillus sp. 균수는 $0.3{\times}10^8 ~ 2.5{\times}10^8 $ cells/ml 범위로 지점별, 조사시기별로 큰 차이가 있었다. 포자수는 $0.3{\times}10^7 ~ 8.4{\times}10^7 $ cells/ml 범위로 3월에는 세 번째 포기조, 5월에는 두 번째 포기조에서 각각 8.5, $5.5{\times}10^7$cells/ml으로 높은 값을 보였다. 총세균수는 DAPH를 측정한 값과 포자수를 합하여 사용하였고, Bacillus속 세균수는 FISH로 계수된 수와 포자수를 합하여 사용하였다. 충세균수에 대한 Bacillus sp.균의 비율은 약 8~60% 범위로 첫 번째 및 세 번째 포기조에서 각각 50%, 35% 이상의 높은 비율을 보였다. B3 공법에서는 Bacillus가 우점하고 있음이 FISH 방법으로 확인되었으며, 반송 슬러지와 하수가 유입되는 첫 번째포기 조에서 그 비율이 높았고, 다음 포기조로 가면서 그 비율이 줄어들고 있었다. Bacillus의 포자수는 뒤의 포기조로 가면서 그 수와 비율이 높아지고 있었다.

• 한국미생물·생명공학회지
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• 제19권2호
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• pp.122-127
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• 1991

A bacterial strain NO. 32 which produced thermostable ${\alpha}$-amylase was isolated from soil and identified to genus of Bacillus. To enhance ${\alpha}$-amylase productivity, a successive mutation of Bacillus sp. No. 32 was attempted with treatment of N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The resulting mutant, Bacillus sp. No. 32H417, which is risistant to refampicin and deficient in spore formation, produced about 90-fold high level of ${\alpha}$-amylase when compared with parental strain. The properties of the enzyme for thermostability were investigated. The optimal temperature and pH for enzyme reaction were 95$^{\circ}C$ and pH6.5, respectively, in the presence of 0.3mM $Ca^{2+}$ as an effective stabilizer.

• 한국미생물·생명공학회지
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• 제19권1호
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• pp.21-24
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• 1991

알칼리 내성 Bacillus sp. YA-14의 chromosomal DNA로부터 분리된 promoter를 subcloning하여 생화학적 특성을 조사하였다. B.subtilis와 promoter 공여균주, Bacillus sp. YA-14에서 promoter의 활성은 포자형성 초기단계에서 급격히 증가하였으며 1.0(w/v) glucose 첨가로 promoter 활성이 억제되었고 c-GMP에 의해 저해되었던 활성이 증가하였다.

• 한국미생물·생명공학회지
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• 제19권6호
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• pp.543-547
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• 1991

토양으로부터 호알칼리성 미생물을 분리하여 동정 한 결과, Bacillus속의 특성을 나타내어 Bacillus sp. SH-8로 명명하였다. Bacillus sp. SH-8은 초기 pH 9.0이상의 알칼리에서만 생육이 가능하였으며 중성 pH에서는 불가능하였다. Bacillus sp. SH-8로부터 U.V.조사에 의해 중성배지에서도 생육이 가능한 변이주를 분리하여 Bacillus sp. SH-8M으로 명명하였으며, 초기 pH 6.9부터 11.0까지 생육이 가능하였다. 따라서 이들 두 균주는 pH 변화에 따른 생육과 효소생산을 비교, 검토하는데 적합한 균주로 생각된다.

• 한국미생물·생명공학회지
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• 제25권5호
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• pp.527-533
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• 1997

From apple skin, we isolated a bacterial strain which strongly inhibited the growth of apple white rot fungus, Botryosphaeria dothidea. The isolated strain, designated as SS279, was identified to be the genus Bacillus. The antifungal activity of Bacillus sp. SS279 was found in the culture filtrate. The production of antifungal substances occurred during logarithmic phase and was the highest when cultures reached the stationary growth phase. The optimum ranges of temperature and pH for its production were 25-30$\circ$C and 4.5-9.0, respectively. The culture filtrate of Bacillus sp. SS279 exhibited a strong inhibitory effect on the spore germination and germ tube elongation of B. dothidea. Autoclaved culture filtrate of Bacillus sp. SS279 showed only a slight decrease in antifungal activity, indicating that the Bacillus sp. SS279 produce heat-stable antifungal substances. In in vivo bioassay, Bacillus sp. SS279 also showed antagonistic activity against apple white rot caused by B. dothidea.

• 한국미생물·생명공학회지
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• 제28권4호
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• pp.219-222
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• 2000

To produce and utilize microbial cyclomaltodextrinase being industrially useful, we isolated an alkalophilic Bacillus strain from soil which was capable of degrading cyclodextrins. The newly isolated strain was aerobic, gram-positive, spore-forming, motile, rod shape(0.2~0.4$\times$1.4~4.4 $\mu\textrm{m}$), and 35.8 mol% of DNA base composition. Based on its morphological, phisiological, and biochemical properties, it was identified as alkalophilic Bacillus sp. KJ-133 and cultivated well in the ranges of $30~40^{\circ}C$ and pH 8.0~9.0 . The cyclomaltodextrinase of the strain showed maximal production after 48h of cultivation at $37^{\circ}C$, and the activity was inhibited by Ag2+, Hg2+, Cu2+, and p-chloromercuribenzoate.

• 환경위생공학
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• 제13권2호
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• pp.34-39
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• 1998

For more convenient and rapidly isolation of Bacillus thuringiensis subsp. israelensis(Bti), 1) heat treatment spore forming bacteria, 2) growth in enrichment culture media for Bacillus sp. and 3) selection of bacteria producing a lecithinase for Bacillus thuringiensis subsp. israelensis, were performed. Spore forming bacteria were counted 4.8 $\times $ 10$^{8}$cells/g soil on NAPGCY media, 9.2 $\times $ 10$^{7}$cells/g soil on NA media, and 3.6 $\times $ 10$^{8}$cells/g soil on NAAC media, respectively. Bacteria producing only a lecithinase were reached at 25.2% on medium contained egg york, bacteria only producing a delta-endotoxin were reached at 23.2% by phase contrast microscope, and bacteria producing a lecithinase & a delta-endotoxin simultaneously were reached at 13.7%. Bacillus thuringiensis which producing a lecithinase and a delta-endotoxin simultaneously among bacteria producing a lecithinase, were reached at 56.5%; A half of Bacillus thuringiensis was produced a delta-endotoxin, but not produced a lecithinase. Among 8 isolates of Bacillus thuringiensis, two strain of Bti which has a mosquito-cidal toxin, were detected by PCR using a specific primer of $\delta $-endotoxin gene from Bti.

• Journal of Microbiology and Biotechnology
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• 제6권1호
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• pp.12-18
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• 1996

A chitosanase-producing bacteria was isolated on chitosan agar plate from soil samples. The strain was spore-forming gram positive bacteria, catalase positive, and rod shape. The strain was identified as Bacillus cereus. The strain did not need an inducer for the synthesis of chitosanase. Chitosanase from Bacillus sp. HW-002 was constitutive enzyme. The optimal medium for the production of the enzyme was composed of 0.5$\%$ sucrose and $1.5\%$ yeast extract-tryptone (1:1 w/w) mixture at pH 6.5. After Bacillus sp. HW-002 was cultivated at $32^{\circ}C$ for 32 h, maximal productivity was gained to be about 27, 200 U/l. Chitosanase from Bacillus sp. HW-002 was a mixed growth-linked metabolite.

• 한국미생물·생명공학회지
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• 제25권5호
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• pp.454-458
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• 1997

To develop a yellow pigment for a food-additive, a strain producing a water-soluble yellow pigment was isolated from phylloplane of tree leaf. The strain PY123 was identified a Bacillus sp. based on morphological and biochemical characterization such as a bacillus form, mortility, spore formation, Gram positive, and catalase production. The pigment production of the strain PY123 was increased about 3 times in patato broth containing 1% sucrose and 0.1 mM CoCl$_{2}$ than in only potato broth after incubation at 30$circ$C, for 2 days. When 0.1 mM CoCl$_{2}$, was added in potato broth containing 1% sucrose at late log phase during incubation of the strain PY123, cell growth was not inhibited, and the period at maximal pigment production of the strain PY123 was about 12hrs faster than in potato broth containing 1% sucrose and 0.1 mM CoCl$_{2}$, simultaniously.

• 생명과학회지
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• 제14권3호
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• pp.467-471
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• 2004

Bacillus sp. SD-10이 생산하는 항균물질을 이용하여 버섯의 갈색무늬병균 및 푸른곰팡이병균에 대한 천연물 버섯병해 방제약재 개발을 위한 기초적 연구를 행하였다. Bacillus sp. SD-10은 35∼4$0^{\circ}C$의 배양온도에서 30∼40 hrs 배양하였을 때 가장 높은 항균활성을 나타내었다. 이 배양액을 이용하여 T. virens의 균사 생육저해 및 포자발아 억제 농도를 검토한 결과, 균사생육은 30 ul 이상 처리 시 뚜렷하게 저해되었으며 포자발아는 평판배지에 5%(v/v) 이상 첨가 시 완전하게 억제되었다. Bacillus sp. SD-10의 배양액으로부터 항균물질을 순수분리하여 UPX-1으로 명명하고, UPX-1을 $^1H-$NMR과 $^{13}C$-NMR 스펙트럼으로 구조를 동정한 결과 탄소 6개로 구성된 당 2개가 2당류 구조로 결합되어 있는 화합물임이 밝혀졌다. UPX-1은 T. virens 뿐 아니라 Pse. tolaassi에도 아주 강한 항균효과를 나타내었다.