• Applied Biological Chemistry
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• 제35권4호
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• pp.242-247
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• 1992

서울시 중량천 하수처리장의 활성오니로부터 총 20주의 pentachlorophenol(PCP) 내성균주들을 분리하여 B. sphaericus B-2와 B. schlegelii B-13를 포함한 Bacillus spp. 9주, Corynebacterium spp. 4주, Staphylococcus aureus S-1 1주, Aeromonas spp. A-19 1주, Arthrobacter LN-3 1주로 동정하였다. 이 균주들은 $200{\sim}300\;ppm$ PCP와 0.1% glucose가 포함된 mineral salt 배지에서 생육하였으며 PCP를 분해하는 종류 13주와 분해하지 못하고 흡착 혹은 흡수하는 종류 7주로 구분되었다. PCP 분해균주들은 대부분 배양 24시간후 생육이 최고에 도달하여 PCP를 약 $75{\sim}90%$ 분해하였으며 PCP 흡착 혹은 흡수하는 균주들은 흡착한 PCP를 배양 24시간 이후 또는 72시간 이후에 방출하였다. PCP 분해균주 B. sphaericus B-2, B. schlegelii B-13의 분해 산물을 TLC를 이용하여 검출한 결과 B. sphaericus B-2의 배양액에서 2-chlorophenol, B. schlegelii B-13에서는 2,4-dichlorophenol로 추정되는 물질과 4종의 분해산물로 보이는 물질들을 검출하였다.

• 한국수산과학회지
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• 제54권5호
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• pp.617-623
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• 2021

The study was conducted to investigate the dietary supplementation of citrus by-product (CBP) and CBP fermented with Bacillus subtilis (BS) or B. pumilus (BP) on growth performance, feed utilization, innate immune responses and disease resistance of Korean rockfish Sebastes schlegelii. Triplicate groups of juvenile fish (initial weight of 22.9 g) were fed one of five experimental diets which were formulated to replace a synthetic vitamin C (L-ascorbyl-2-polyphosphate, LAPP) with CBP or fermented CBP. A basal diet without LAPP and CBP was used as the control diet (Con). At the end of the 13 weeks feeding trial, growth performance, feed utilization and survival of fish fed the Con were significantly lower than those of fish fed the LAPP, CBP and fermented CBP diets. Fish fed the Con were significantly lower in lysozyme activity than fish fed LAPP, CBP and fermented CBP diets. In a challenge test against Edwardsiella tarda, cumulative mortality of fish fed LAPP, CBP and fermented CBP diets was significantly lower (20-30%) than that of the Con (60%). The findings in this study indicate that the CBP and/or fermented CBP can be a promising vitamin C source for LAPP replacement in diet for Korean rockfish.

• 한국미생물·생명공학회지
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• 제25권6호
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• pp.623-629
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• 1997

The studies of isolation and physiological characteristics of mesophilic or thermophilic bacteria from thermophilic oxic process (TOP) treating Chinese restaurant wastes were conducted. Chinese restaurant wastes were consist of moisture; 75.8%, solids; 24.2% and ash; 0.49%. The volatile solid was about 99% of total dry solids. In wastes used in this experiment, there was content of crude protein; 4.47%, crude lipid; 3.56%, free sugar, 0.4% , crude starch; 10.34% and crude fiber 3.14%, respectively. And then it has about 4,970 kcal/kg-dry solid of Chinese restaurant wastes. From TOP treating the chinese restaurant wastes, 37 strains of mesophilic or thermophilic bacteria were primarily isolated using medium used for the isolation and among them 6 strains of thermophilic and 7 strains of mesophilic bacteria were selected by testing the activities of amylase, cellulase, protease and lipase. TB-1, TB-9 as thermophilic bacteria and MB-15-1, 15-2, MB23 as mesophilic bacteria having strong enzyme activity were selected among isolated strains. All selected strains reduced nitrate to nitrite and they utilized glucose, manose, manitol, and maltose as carbon source. From these MB15-2 was identified as Bacillus cereus, TB1, Bacillus licheniformis and TB9; Bacillus schlegelii.