• 미생물학회지
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• 제30권5호
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• pp.339-346
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• 1992

수영만에서 분리된 Bacillus cereus group LS-1 의 형태학적, 생화학적 성상 및 지방산 조성 분석을 통하여 세균학적인 특성을 밝혔다. B. cereus group LS-1 은 면양혈구배지에서 비용혈성의 점조성 집략과 convex 하고 가장자리가 규칙적인 둥근형태로 운동성이 없고 glucose, maltose, sucrose 와 gelatin 을 이용하고 trehalose 와 salicin 을 분해하지 않으며 6.5% NaCl 에서 자라지 않는 Gram 양성의 중심성 아포형성간균으로 표준균주 B. cereus group 과 다소 차이를 나타내었다. 지방산 조성 분석에서 chain 의 길이가 $C_{12}$ 에서 $C_{17}$로 iso $C_{15}$와 iso $C_{13}$의 branched chain 이 우점하는 B. cereus group 의 전형적인 특징을 나타내었으며 $nC^{15}$가 검출되지 않는 B.mycoides GC subgroup B 로 0.312 의 similarity index(SI) 를 지칭하여 다른 연구 결과와도 일치하였다. 한편 API system (API 50 CHB & API 20E) 의 ATB computer profile 에서 "Doubful Profile" 99.7% 의 B. firmus 로 나타내어 큰차이를 나타내었다. 67 개의 biochemical character 로 B. mycoids S-12 는 각각 42%, 42% 59% 와 52% 의 similarity matrix 를 나타내었다. B. cereus group 간의 아주 낮은 similarity 를 나타내어 상당한 차이가 있음을 인식하였다. 따라서 key test 와 지방산 조성등을 종합하여 볼때 B. ceresu group 중의 B. mycoides 로 새로운 biotype 인 것으로 사료되며 지방산 조성 분석으로 동정함이 훨씬 용이하였다.

• Journal of Microbiology and Biotechnology
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• 제11권1호
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• pp.65-71
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• 2001

A gene from Paenibacillus sp. KCTC 8848P encoding ${\beta}-amylase$ was cloned and expressed in Escherichia coli. The Paenibacillus ${\beta}-amylase$ gene cosisted of a 2,409-bp open reading frame without a translational stop codon, encoding a protein of 803 amino acids. The presumed ribosime-binding site, GGAGG, was located 10 bp upstream from the TTG initiation codon. The deduced amino acid sequence of the ${\beta}-amylase$ gene had a 95% similarity to the ${\beta}-amylase$ of Bacillus firmus. The ${\beta}-amylase$ gene was introduced into wild-type strains of Saccharomyces cerevisiae using a linearized yeast integrating vector containing a geneticin resistance gene and its product was secreted into the culture medium.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2010년도 정기총회 및 추계학술발표회
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• pp.18-18
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• 2010

Molecular insights on the role of plant growth promoting rhizobacteria (PGPR) in potato tuberization are reported in the present study. The PGPRwere isolated from the soil collected from potato fields of Highland Agricultural Research Centre, Pyeongchang, Korea and they were identified to the genus level based on the 16S rRNA sequence analysis. These PGPR were heat-killed, filtered and the filtrates were addedindividually at a concentration of $10^7\;cfu\;mL^{-1}$ in MS (Murashige and Skoog's) medium supplemented with 7% (w/v) sucrose to study their influence on in vitro potato tuberization. Tuber initiation occurred early in untreated control, while tuber growth was pronounced in case of PGPR treatments. The control explants showed tuber formation as a result of sub-apical swelling of stolons while several sessile tubers formed directly in the axils of nodal cuttings in case of PGPR treatments, which is an indication of strong induction for tuberization. Theexplants cultured on MS medium supplemented with bacterial isolate 6 (Bacillus firmus strain 40) showed highest average tuber yield (Ca. 12.56 g per treatment) after 30 days of culture, which was 3 folds increase over the untreated control. A significant increase in lipoxygenase (LOX1) mRNA expression and activity of LOX enzyme were also detected in the tubers induced on PGPR treatments as compared to untreated control. This LOX expression level correlated with increased tuber growth and tuber yield. Further studies focused on the role of bacteria cell wall components, growth regulators and signal molecules released by PGPR are under investigation to elicit clues for PGPR-mediated signal pathway controlling potato tuberization.