• Asian Pacific Journal of Cancer Prevention
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• v.13 no.11
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• pp.5785-5790
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• 2012

The use of chemotherapeutics induces cardiotoxicity and affects immune functions, therefore development of combinatorial agents against cardiotoxicity and immunosuppression needs to be explored. Previous studies of the hexane insoluble fraction (HIF) of an ethanolic extract of Ficus septica leaves showed anticancer effects singly and in combination with doxorubicin on T47D breast cancer cells. In this present study, it was evaluated for its immunomodulatory activities in doxorubicin-treated rats. Thirty male Sprague Dawley rats were divided into five groups consisting of six rats each as follows: Group 1, receiving oral saline 10 ml/kg BW (control group); Group 2, receiving HIF dose 750 mg/kg BW orally, once daily; Group 3, receiving HIF dose 1.500 mg/kg BW orally, once daily; Group 4, given oral saline 10 ml/kg BW (normal group); Group 5, receiving HIF dose 1.500 mg/kg BW orally, once daily. The rats of group 1-3 were intramuscularly administered with doxorubicin at a dose of 4.67 mg/kg BW at the days 1 and 4 to suppress immune functions. Concomitantly, the rats were treated with saline or HIF for seven consecutive days (1 to 7). Treatment of HIF succeeded in reducing side effects of doxorubicin based on increasing lymphocyte density and phagocytosis activity and capacity of macrophages, as well as increasing the CD8+ blood level and decreasing spleen IL-10 expression. Hexane insoluble fraction of of ethanolic extract of Ficus septica leaves has potential as a protective agent combined with doxorubicin.

• Korean Journal of Applied Biomechanics
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• v.18 no.1
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• pp.31-38
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• 2008

The purpose of this study was to investigate the effects of anterior cruciate ligament (ACL) injury prevention program on the ground reaction force during the rebound in female highschool basketball players. Sixteen highschool female basketball players (ages 16 to 18 years) were participated in this study. Eight of these players participated in a 8-week ACL injury prevention program. Before and After ACL injury prevention program, initial peak value(%BW), maximum peak value(%BW), impulse(%BW sec), and loading rate(N/sec) were measured in the players. The experiment group after ACL injury prevention program showed significant decreased initial peak value, impulse, and loading rate.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.32 no.1
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• pp.16-23
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• 1996

Spatial distribution characteristics, volume backscattering strength and species composition of midwater trawling catch was analyzed biological and acoustical characteristics of anchovy shoal, using a high resolution echo - sounder at the south region of East sea of Korea. 1) In the survey site A of Lat.35$^{\circ}$55'N, Long.129$^{\circ}$45'E, the anchovy shoal of small to middle size with the horizontal range of 10~25m and large size with the horizontal range of 40~50m were distributed together. However in the survey site B of Lat.35$^{\circ}$38'N, Long.129$^{\circ}$40'E, the anchovy shoal was observed to be mainly small size which about 78% of the detected shoal. Another was that the anchovy shoal with the vertical range of 2~8m occupied about 68.6% in the survey site A and that of 6~12m occupied about 42.5% in the survey site B. The mainly the site A and B were found to be 10~50m super (2), 64.5% and 20~80m super (2), 66%, respectively. 2) The volume backscattering strength in the site A and B were observed to be -44.0~ -28.0dB, respectively. In the site A, the backscattering strength of -40.0~ -30.0dB was analyzed about 41.4%. 3) Most of total anchovy shoal was concentrated in the water layer of 50~100m in depth with 15.3~18.5$^{\circ}C$, 34.0~34.3$\textperthousand$ in the survey site A and 14.2~16.4$^{\circ}C$, 34.1~34.2$\textperthousand$ in the survey site B. 4) Mean total length(TL) and body weight(BW) of anchovy in the survey site A were 9.9cm and 4.4g respectively, with TL-BW relationship of BW=0.0007T$L^3.65$super (3.85). In site B, mean total length(TL) and body weight(BW) were 11.2cm and 8.7g, with TL-BW relationship of BW=0.0023T$L^3.36$.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.9
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• pp.1293-1302
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• 2014

The aim of study was to determine the energy requirements for maintenance and growth of forty-one Saanen, intact male kids with initial body weight (BW) of $5.12{\pm}0.19$ kg. The baseline (BL) group consisted of eight kids averaging $5.46{\pm}0.18$ kg BW. An intermediate group consisted of six kids, fed for ad libitum intake, that were slaughtered when they reached an average BW of $12.9{\pm}0.29$ kg. The remaining kids (n = 27) were randomly allocated into nine slaughter groups (blocks) of three animals distributed among three amounts of dry matter intake (DMI; ad libitum and restricted to 70% or 40% of ad libitum intake). Animals in a group were slaughtered when the ad libitum-treatment kid in the group reached 20 kg BW. In a digestibility trial, 21 kids (same animals of the comparative slaughter) were housed in metabolic cages and used in a completely randomized design to evaluate the energetic value of the diet at different feed intake levels. The net energy for maintenance ($NE_m$) was $417kJ/kg^{0.75}$ of empty BW (EBW)/d, while the metabolizable energy for maintenance ($ME_m$) was $657kJ/kg^{0.75}$ of EBW/d. The efficiency of ME use for NE maintenance ($k_m$) was 0.64. Body fat content varied from 59.91 to 92.02 g/kg of EBW while body energy content varied from 6.37 to 7.76 MJ/kg of EBW, respectively, for 5 and 20 kg of EBW. The net energy for growth ($NE_g$) ranged from 7.4 to 9.0 MJ/kg of empty weight gain by day at 5 and 20 kg BW, respectively. This study indicated that the energy requirements in goats were lower than previously published requirements for growing dairy goats.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.2
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• pp.237-244
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• 2018

Objective: Piglet pre-weaning mortality is an important variable indicating the efficacy of farrowing management and animal well-being during lactation. The present study determined the association of newborn traits measured soon after birth with piglet pre-weaning mortality and growth. Methods: In total, 805 piglets born from 57 multiparous sows were investigated. Their blood oxygen saturation, blood glucose and rectal temperature at 24 h after birth (RT24h) were monitored. Birth order, sex, skin color, integrity of the umbilical cord, attempts to stand and birth intervention were monitored. Piglets were weighed at day 0, 7, and 21 to evaluate average daily gain (ADG). Results: Piglet pre-weaning mortality for lactation period was 12.6% and cumulative mortality during the first 7 days of age was 8.6%. A higher proportion of piglets with pale skin color died compared to piglets with normal skin color (26.7% vs 7.7%, p<0.001). A higher (p<0.001) proportion of piglets that attempted to stand after 5 min (38.5%) died compared to piglets that attempted to stand within 1 min (6.3%) after birth. Piglet body weight at birth ($BW_B$), blood glucose and the number of piglets born alive (BA) were correlated with ADG (p<0.05). Piglets with $BW_B$ <1.30 kg had higher (p<0.001) mortality rate than piglets with $BW_B{\geq}1.80 kg$ (19.0% vs 3.3%) and piglets with $BW_B$ 1.30 to 1.79 kg (4.0%). Piglet with RT24h <$37.0^{\circ}C$ had higher (p<0.001) mortality rate (86.2%) than piglets with RT24h >$38.5^{\circ}C$ (3.9%). Conclusion: Low $BW_B$ and low RT24h compromise piglet survival during the lactation period in the tropical conditions. Piglets in the litters with a high BA, low $BW_B$ and low blood glucose have reduced ADG.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.5
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• pp.681-687
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• 2015

We investigated the effects of feeding rate on the growth performance of growing and sub-adult olive flounder Paralichthys olivaceus, and the optimum feeding rate at high water temperature (25-31℃). In experiment I, two replicated groups of fish (113 g) were fed a commercial diet (CD) at rates of 0, 0.7, 1.2, 1.7, 2.2, and 2.4% (satiation) body weight (BW) per day for 4 weeks. In experiment II, two replicated groups of fish (313 g) were fed a CD feeding rates of 0, 0.3, 0.5, 0.7, 0.9, and 1.0% (satiation) BW per day for 4 weeks. In experiment I, the weight gain (WG) and specific growth rate (SGR) of fish fed at 0.7 and 0.9% BW per day was significantly higher than that of unfed fish (0%) and fish fed at 0.3, 0.5, and 1.0% (satiation) BW per day. In experiment II, the WG and SGR increased significantly as feeding rate increased from 0 to 2.2% BW per day, but decreased in the satiation group (2.4%). Broken-line regression analysis of WG showed that the optimum feeding rate of growing and sub-adult olive flounder were 1.98% and 0.55% BW per day, respectively, at the high water temperature.

• Fisheries and Aquatic Sciences
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• v.17 no.2
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• pp.229-247
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• 2014

Seven feeding trials were conducted to determine the effects of feeding rate and frequency in the Korean rockfish Sebastes schlegeli at seven different water temperatures. Two feeding-rate experiments for 5-g Korean rockfish at $17^{\circ}C$ and $20^{\circ}C$; three feeding rate experiments for 16-g Korean rockfish at $16^{\circ}C$ and $24^{\circ}C$; and finally, two feeding frequency experiments for 93- and 100-g at $15^{\circ}C$ and $20^{\circ}C$ were conducted. Twenty fish averaging $5.5{\pm}0.2$ g ($mean{\pm}SD$) were randomly distributed into 18 indoor tanks containing 40-L seawater from a semi-recirculation system. Fish were fed a commercial diet for 4 weeks at water temperatures of $17^{\circ}C$ and $20^{\circ}C$. Two feeding trials were conducted to determine the optimum feeding frequency in growing Korean rockfish reared at temperatures of $15^{\circ}C$ and $19^{\circ}C$. Broken line regression analysis of weight gain (WG) indicated that the optimum feeding rates of 5-g growing Korean rockfish were 4.48% (BW/day; BW, body weight) at $17^{\circ}C$ and 4.83% (BW/day) at $20^{\circ}C$. Broken line regression analysis of WG showed optimum feeding rates of 16-g juvenile Korean rockfish of 3.41% (BW/day) at $16^{\circ}C$, 3.75% (BW/day) at $20^{\circ}C$, and 3.34% (BW/day) at $24^{\circ}C$. The biological performance, results, along with morphological indices and serological characteristics, suggest that a feeding frequency of 1 meal/day was optimal to improve WG in growing Korean rockfish grown from 93 to 133 g at a water temperature of $15^{\circ}C$, and 100 to 132 g at $19^{\circ}C$.

• Animal cells and systems
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• v.16 no.5
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• pp.391-399
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• 2012

We cloned cDNA-encoding arginine vasotocin (AVT) from the brain of the cinnamon clownfish Amphiprion melanopus, and that was predicted to encode a protein of 153 amino acids. We examined changes in the expression of AVT mRNA in the brain and arginine vasotocin receptor (AVTR) mRNA and osmotic stress transcription factor 1 (OSTF1) mRNA in the gills of the cinnamon clownfish using quantitative real-time PCR in an osmotically changing environment (seawater (35 psu) ${\rightarrow}$ brackish water (BW, 17.5 psu) and BW with prolactin [PRL]). The expression of AVT, AVTR, and OSTF1 mRNA in the brain and gills increased after transfer to BW, and the expression was repressed by PRL treatment. AVT-immunoreactive cells were almost consistently observed in the telencephalon. The plasma $Na^+$ and $Cl^-$ levels decreased in BW, but the level of this parameter increased in BW with PRL treatme during salinity change. These results suggest that AVT, AVTR, and OSTF1 play important roles in hormonal regulation in osmoregulation organs, and that PRL improves the hyperosmoregulatory ability of cinnamon clownfish in BW environment.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.6
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• pp.878-883
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• 2002

Due to rising trend of beef demand in Asia in the next two decades it is necessary to find additional sources of beef supply. In most Southeast Asian countries, male dairy and swamp buffalo have not yet been raised for a primary purpose of quality beef production. This study was aimed to compare growth and feeding performances as well as economic returns from feeding male dairy, beef cattle and swamp buffalo for quality beef. Thirty-six animals, 12 of each breed group, were used in feeding trial to compare the cost of beef production. Two levels of concentrate feeding, 1.75% of body weight (BW) and 1.00% of BW, were used for each breed group in order to compare feeding methods i.e. high and low levels. Within each breed group two animals of similar initial BW were randomly assigned to the two levels of feeding. The animals were fed from about 150 kg BW until reaching the final weight of about 400 kg. The results from this study showed that under the prevailing economic conditions in Thailand the cost of beef production from buffalo was lowest due to very low cost of feeder stocks, followed by dairy and beef. However, the cost of feeding per kg of BW gain was lowest in beef and highest in buffalo i.e. when disregarding the differences in cost of feeder stocks. Beef calves grew faster than dairy and buffalo, with better feed efficiencies. The results indicated that beef cattle could be more suitable for beef production for high-quality beef market, while buffalo could be more suitable for small farms where high roughage feeding is common. Male dairy calves appeared to require higher level of concentrate feeding than 1% BW in order to maintain good body conditions.

• The Journal of the Korean Society for Microbiology
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• v.18 no.1
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• pp.53-58
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• 1983

Enterotoxigenic E. coli is one of causative agents of the infantile diarrhea and traveler's diarrhea. A modified infant mouse assay(IMA) was developed for the detection of heat stable enterotoxin (ST) of E. coli isolated from diarrheal and control infants and assay system was established with using enterotoxin producing reference strains. The supernatant of the 24 hour-shaking culture of E. coli in Casamino Acid Yeast Extract Salt Broth(CYES-2) was ingested orally into the 2-4 day old ICR mice. After the mice were kept at $25^{\circ}C$ for 4 hours, they were sacrificed and the gut weight body weight ratio(GW/BW) was taken as the index of fluid accumulation induced by heat stable enterotoxin of E. coli. The results obtained were as follows; 1. The GW/BW responses of IMA tested with enterotoxin reference strains of E. coli(E. coli O148H28:$ST^+LT^+$, E. coli $O78H^-:ST^+LT^+$, E. coli O15H11:$ST^-LT^+$, E. coli O1H7:$ST^-LT^-$) appeared ta be ST dose-dependent, and not LT-dependent. From the dose-response curve, $25{\mu}l$ of culture supernatant was determined as test amount of the IMA. 2. Frequency distribution of IMA result from 643 strain of E. coli showed normal distribution at low GW/BW ratio and dispersed pattern at high GW/BW ratio. The GW/BW ratios of $0.056{\pm}0.004(mean{\pm}SD)$ of normal distribution which distributed from 0.044 to 0.068(P<0.01) was considered as ST negative. Thus the GW/BW ratio above 0.069 could be regarded as ST positive.